P06481 (US9_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Envelope protein US9

Alternative name(s):
10 kDa protein

Gene names

ORF Names:

US9

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the anterograde spread of the infection throughout the host nervous system. Together with the gE/gI heterodimer, US9 is involved in the sorting and transport of viral structural components toward axon tips. Ref.5 Ref.6
Subcellular location	Virion membrane; Single-pass type II membrane protein. Host Golgi apparatus membrane; Single-pass type II membrane protein. Host smooth endoplasmic reticulum membrane; Single-pass type II membrane protein Probable. Host cell membrane; Single-pass type II membrane protein Potential. Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN), maybe through an interaction with PACS-1 sorting protein Potential. Ref.5 Ref.7 Ref.8
Post-translational modification	Phosphorylated on serines within the acidic cluster, possibly by host CK2 Potential. Phosphorylation determines whether endocytosed viral US9 traffics to the trans-Golgi network or recycles to the cell membrane Potential.
Sequence similarities	Belongs to the alphaherpesvirinae envelope protein US9 family.

Ontologies

Keywords
Cellular component	Host Golgi apparatus Host cell membrane Host endoplasmic reticulum Host membrane Membrane Viral envelope protein Virion
Domain	Signal-anchor Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	host cell Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell smooth endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW viral tegument Inferred from electronic annotation. Source: InterPro virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 90	90	Envelope protein US9		PRO_0000116132
Regions
	Topological domain	1 – 67	67	Intravirion By similarity
	Transmembrane	68 – 88	21	Helical; Signal-anchor for type II membrane protein; By similarity
	Topological domain	89 – 90	2	Virion surface By similarity
	Region	30 – 39	10	Acidic
	Motif	21 – 24	4	Internalization motif Potential
	Compositional bias	56 – 63	8	Arg-rich (basic)
Amino acid modifications
	Modified residue	34	1	Phosphoserine; by host CK2 Potential
	Modified residue	36	1	Phosphoserine; by host CK2 Potential
Natural variations
	Natural variant	29	1	V → A in strain: Nonneuroinvasive mutant HF10.

Sequences

	Sequence		Length	Mass (Da)
	P06481 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: F4D4DC2608BB38F7	FASTA	90	10,027
	10 20 30 40 50 60 MTSRLSDPNS SARSDMSVPL YPTASPVSVE AYYSESEDEA ANDFLVRMGR QQSVLRRRRR 70 80 90 RTRCVGMVIA CLLVAVLSGG FGALLMWLLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dolan A., Donald S., Rixon F.J. J. Mol. Biol. 181:1-13(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus." Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y. Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nonneuroinvasive mutant HF10.
[4]	"Herpes simplex virus type 1 bacterial artificial chromosome." Cunningham C., Davison A.J. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 17 syn+.
[5]	"Viral regulation of the long distance axonal transport of herpes simplex virus nucleocapsid." LaVail J.H., Tauscher A.N., Sucher A., Harrabi O., Brandimarti R. Neuroscience 146:974-985(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. Strain: F.
[6]	"Herpes simplex virus gE/gI and US9 proteins promote transport of both capsids and virion glycoproteins in neuronal axons." Snyder A., Polcicova K., Johnson D.C. J. Virol. 82:10613-10624(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: F.
[7]	"Comprehensive characterization of extracellular herpes simplex virus type 1 virions." Loret S., Guay G., Lippe R. J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: F.
[8]	"Comparison of the pseudorabies virus Us9 protein with homologs from other veterinary and human alphaherpesviruses." Lyman M.G., Kemp C.D., Taylor M.P., Enquist L.W. J. Virol. 83:6978-6986(2009) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L00036 Genomic DNA. Translation: AAA96679.1. X14112 Genomic DNA. Translation: CAA32274.1. X02138 Genomic DNA. Translation: CAA26063.1. DQ889502 Genomic DNA. Translation: ABI63528.1. FJ593289 Genomic DNA. Translation: ACM62299.1.
PIR	QQBE79. A05241.
RefSeq	NP_044672.1. NC_001806.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703452.
Phylogenomic databases
ProtClustDB	CLSP2510183.
Family and domain databases
InterPro	IPR009278. Herpes_US9. [Graphical view]
Pfam	PF06072. Herpes_US9. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

US9_HHV11

Accession

Primary (citable) accession number: P06481
Secondary accession number(s): B9VQK4, Q09I67

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program