P06479 (KITH_HHV1S) Reviewed, UniProtKB/Swiss-Prot
Last modified October 16, 2013. Version 56. History...
Names and origin
|Protein names||Recommended name:|
|Organism||Human herpesvirus 1 (strain SC16) (HHV-1) (Human herpes simplex virus 1)|
|Taxonomic identifier||10309 [NCBI]|
|Taxonomic lineage||Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus ›|
|Virus host||Homo sapiens (Human) [TaxID: 9606]|
|Sequence length||376 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome By similarity.
ATP + thymidine = ADP + thymidine 5'-phosphate.
Homodimer By similarity.
Used in molecular biology as a selectable marker to identify transfected eukaryotic cells. Used in cancer suicide gene therapy to selectively kill transformed cells.
Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valaciclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HSV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions.
Belongs to the herpesviridae thymidine kinase family.
|Biological process||DNA synthesis|
|Gene Ontology (GO)|
Inferred from electronic annotation. Source: UniProtKB-KWTMP biosynthetic process
Inferred from electronic annotation. Source: InterPro
Inferred from electronic annotation. Source: UniProtKB-KWthymidine kinase activity
Inferred from electronic annotation. Source: UniProtKB-EC
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 376||376||Thymidine kinase||PRO_0000175073|
|Nucleotide binding||56 – 63||8||ATP By similarity|
|Active site||83||1||Proton acceptor Potential|
|Binding site||101||1||Substrate By similarity|
|Binding site||125||1||Substrate By similarity|
|Binding site||172||1||Substrate By similarity|
|Binding site||216||1||ATP By similarity|
|||"Evidence that the 'active centre' of the herpes simplex virus thymidine kinase involves an interaction between three distinct regions of the polypeptide."|
Darby G., Larder B.A., Inglis M.M.
J. Gen. Virol. 67:753-758(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
|X03764 Genomic DNA. Translation: CAA27395.1.|
|PIR||KIBE16. A27240. |
3D structure databases
|SMR||P06479. Positions 46-376. |
Protocols and materials databases
Family and domain databases
|InterPro||IPR001889. Herpes_TK. |
|Pfam||PF00693. Herpes_TK. 1 hit. |
|SUPFAM||SSF52540. SSF52540. 1 hit. |
|Accession||Primary (citable) accession number: P06479|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families