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P06399

- FIBA_RAT

UniProt

P06399 - FIBA_RAT

Protein

Fibrinogen alpha chain

Gene

Fga

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei36 – 372Cleavage; by thrombin; to release fibrinopeptide A

    GO - Biological processi

    1. blood coagulation Source: RGD
    2. platelet activation Source: InterPro
    3. protein polymerization Source: InterPro
    4. signal transduction Source: InterPro
    5. tissue regeneration Source: RGD

    Keywords - Biological processi

    Blood coagulation, Hemostasis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fibrinogen alpha chain
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Fga
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2603. Fga.

    Subcellular locationi

    GO - Cellular componenti

    1. blood microparticle Source: RGD
    2. cytoplasm Source: RGD
    3. fibrinogen complex Source: InterPro

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19191 PublicationAdd
    BLAST
    Peptidei20 – 3617Fibrinopeptide APRO_0000009039Add
    BLAST
    Chaini37 – 782746Fibrinogen alpha chainPRO_0000009038Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi48 – 48InterchainPROSITE-ProRule annotation
    Disulfide bondi56 – 56Interchain (with beta chain)PROSITE-ProRule annotation
    Disulfide bondi65 – 65Interchain (with C-49 in gamma chain)PROSITE-ProRule annotation
    Disulfide bondi69 – 69Interchain (with beta chain)PROSITE-ProRule annotation
    Disulfide bondi181 – 181Interchain (with C-165 in gamma chain)PROSITE-ProRule annotation
    Disulfide bondi185 – 185Interchain (with beta chain)PROSITE-ProRule annotation
    Disulfide bondi404 ↔ 434PROSITE-ProRule annotation
    Glycosylationi602 – 6021N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The alpha chain is not glycosylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    Phosphorylation sites are present in the extracellular medium.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP06399.
    PRIDEiP06399.

    Miscellaneous databases

    PMAP-CutDBP06399.

    Expressioni

    Gene expression databases

    GenevestigatoriP06399.

    Interactioni

    Subunit structurei

    Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP06399.
    SMRiP06399. Positions 46-93, 131-215, 586-782.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini539 – 780242Fibrinogen C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili68 – 547480By similarityAdd
    BLAST

    Domaini

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

    Sequence similaritiesi

    Contains 1 fibrinogen C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Signal

    Phylogenomic databases

    eggNOGiNOG114889.
    HOGENOMiHOG000285947.
    HOVERGENiHBG005668.
    InParanoidiP06399.
    PhylomeDBiP06399.

    Family and domain databases

    Gene3Di3.90.215.10. 1 hit.
    4.10.530.10. 1 hit.
    InterProiIPR014716. Fibrinogen_a/b/g_C_1.
    IPR014715. Fibrinogen_a/b/g_C_2.
    IPR002181. Fibrinogen_a/b/g_C_dom.
    IPR012290. Fibrinogen_a/b/g_coil_dom.
    IPR021996. Fibrinogen_aC.
    IPR020837. Fibrinogen_CS.
    [Graphical view]
    PfamiPF08702. Fib_alpha. 1 hit.
    PF12160. Fibrinogen_aC. 1 hit.
    PF00147. Fibrinogen_C. 1 hit.
    [Graphical view]
    SMARTiSM00186. FBG. 1 hit.
    [Graphical view]
    SUPFAMiSSF56496. SSF56496. 1 hit.
    PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
    PS51406. FIBRINOGEN_C_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P06399-1) [UniParc]FASTAAdd to Basket

    Also known as: Alpha-E

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLSLRVACLI LSLASTVWTA DTGTTSEFIE AGGDIRGPRI VERQPSQCKE    50
    TDWPFCSDED WNHKCPSGCR MKGLIDEANQ DFTNRINKLK NSLFDFQKNN 100
    KDSNSLTRNI MEYLRGDFAN ANNFDNTFGQ VSEDLRRRIQ ILKRKVIEKA 150
    QQIQVLQKDV RDQLIDMKRL EVDIDIKIRS CKGSCSRSVS REINLKDYEG 200
    QQKQLEQVIA KDLLPAKDRQ YLPAIKMSPV PDLVPGSFKS QLQEGPPEWK 250
    ALTEMRQMRM ELERPGKDGA SRGDLPGDSR GDSATRGPGS KIENPMTPGH 300
    GGSGYWRPGS SGSGSDGNWG SGTTGSDDTG TWGAGSSRPS SGSGNLKPSN 350
    PDWGEFSEFG GSSSPATRKE YHTGKLVTSK GDKELLIGNE KVTSTGTSTT 400
    RRSCSKTITK TVLGNDGHRE VVKEVVTSDD GSDCGDGMDL GLTHSFSGRL 450
    DELSRMHPEL GSFYDSRFGS LTSNFKEFGS KTSDSDIFTD IENPSSHVPE 500
    FSSSSKTSTV RKQVTKSYKM ADEAASEAHQ EGDTRTTKRG RARTMRDCDD 550
    VLQTHPSGAQ NGIFSIKLPG SSKIFSVYCD QETSLGGWLL IQQRMDGSLN 600
    FNRTWQDYKR GFGSLNDKGE GEFWLGNDYL HLLTLRGSVL RVELEDWAGK 650
    EAYAEYHFRV GSEAEGYALQ VSSYQGTAGD ALMEGSVEEG TEYTSHSNMQ 700
    FSTFDRDADQ WEENCAEVYG GGWWYNSCQA ANLNGIYYPG GTYDPRNNSP 750
    YEIENGVLWV PFRGADYSLW AVRMKIRPLV GQ 782
    Length:782
    Mass (Da):86,686
    Last modified:October 1, 1996 - v3
    Checksum:i744834DAE76D34C2
    GO
    Isoform 2 (identifier: P06399-2) [UniParc]FASTAAdd to Basket

    Also known as: Alpha

    The sequence of this isoform differs from the canonical sequence as follows:
         547-550: DCDD → GIHA
         551-782: Missing.

    Note: Major isoform.

    Show »
    Length:550
    Mass (Da):60,490
    Checksum:i24C2E1470DD2C7BF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 345EAGGD → DEGAG AA sequence 1 PublicationCurated
    Sequence conflicti140 – 1401Q → E no nucleotide entry (PubMed:4046033)Curated
    Sequence conflicti212 – 2121D → E no nucleotide entry (PubMed:4046033)Curated
    Sequence conflicti270 – 2767ASRGDLP → LREEIYQ(PubMed:4046033)Curated
    Sequence conflicti473 – 4731S → K in AAA41158. (PubMed:3817019)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei547 – 5504DCDD → GIHA in isoform 2. 1 PublicationVSP_001533
    Alternative sequencei551 – 782232Missing in isoform 2. 1 PublicationVSP_001534Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86561 Genomic DNA. Translation: CAA60264.1.
    X86561 Genomic DNA. Translation: CAA60263.1.
    M35601 mRNA. Translation: AAA41158.1.
    PIRiI53408.
    UniGeneiRn.98846.

    Genome annotation databases

    UCSCiRGD:2603. rat. [P06399-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86561 Genomic DNA. Translation: CAA60264.1 .
    X86561 Genomic DNA. Translation: CAA60263.1 .
    M35601 mRNA. Translation: AAA41158.1 .
    PIRi I53408.
    UniGenei Rn.98846.

    3D structure databases

    ProteinModelPortali P06399.
    SMRi P06399. Positions 46-93, 131-215, 586-782.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P06399.
    PRIDEi P06399.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2603. rat. [P06399-1 ]

    Organism-specific databases

    RGDi 2603. Fga.

    Phylogenomic databases

    eggNOGi NOG114889.
    HOGENOMi HOG000285947.
    HOVERGENi HBG005668.
    InParanoidi P06399.
    PhylomeDBi P06399.

    Miscellaneous databases

    PMAP-CutDB P06399.
    PROi P06399.

    Gene expression databases

    Genevestigatori P06399.

    Family and domain databases

    Gene3Di 3.90.215.10. 1 hit.
    4.10.530.10. 1 hit.
    InterProi IPR014716. Fibrinogen_a/b/g_C_1.
    IPR014715. Fibrinogen_a/b/g_C_2.
    IPR002181. Fibrinogen_a/b/g_C_dom.
    IPR012290. Fibrinogen_a/b/g_coil_dom.
    IPR021996. Fibrinogen_aC.
    IPR020837. Fibrinogen_CS.
    [Graphical view ]
    Pfami PF08702. Fib_alpha. 1 hit.
    PF12160. Fibrinogen_aC. 1 hit.
    PF00147. Fibrinogen_C. 1 hit.
    [Graphical view ]
    SMARTi SM00186. FBG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56496. SSF56496. 1 hit.
    PROSITEi PS00514. FIBRINOGEN_C_1. 1 hit.
    PS51406. FIBRINOGEN_C_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Fibrinogen alpha genes: conservation of bipartite transcripts and carboxy-terminal-extended alpha subunits in vertebrates."
      Fu Y., Cao Y., Hertzberg K.M., Grieninger G.
      Genomics 30:71-76(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Evolution and structure of the fibrinogen genes. Random insertion of introns or selective loss?"
      Crabtree G.R., Comeau C.M., Fowlkes D.M., Fornace A.J. Jr., Malley J.D., Kant J.A.
      J. Mol. Biol. 185:1-19(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
    3. "Studies on fibrinopeptides from mammals."
      Blombaeck B., Blombaeck M., Grondahl N.J.
      Acta Chem. Scand. 19:1789-1791(1965)
      Cited for: PROTEIN SEQUENCE OF 20-36.
    4. "Molecular cloning of mRNA sequences transiently induced during rat liver regeneration."
      Sobczak J., Lotti A.-M., Taroux P., Duguet M.
      Exp. Cell Res. 169:47-56(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 458-550 (ISOFORM 2).
      Strain: Wistar.
      Tissue: Liver.

    Entry informationi

    Entry nameiFIBA_RAT
    AccessioniPrimary (citable) accession number: P06399
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3