SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P06399

- FIBA_RAT

UniProt

P06399 - FIBA_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Fibrinogen alpha chain
Gene
Fga
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei36 – 372Cleavage; by thrombin; to release fibrinopeptide A

GO - Biological processi

  1. blood coagulation Source: RGD
  2. platelet activation Source: InterPro
  3. protein polymerization Source: InterPro
  4. signal transduction Source: InterPro
  5. tissue regeneration Source: RGD
Complete GO annotation...

Keywords - Biological processi

Blood coagulation, Hemostasis

Names & Taxonomyi

Protein namesi
Recommended name:
Fibrinogen alpha chain
Cleaved into the following 2 chains:
Gene namesi
Name:Fga
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2603. Fga.

Subcellular locationi

GO - Cellular componenti

  1. blood microparticle Source: RGD
  2. cytoplasm Source: RGD
  3. fibrinogen complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 Publication
Add
BLAST
Peptidei20 – 3617Fibrinopeptide A1 Publication
PRO_0000009039Add
BLAST
Chaini37 – 782746Fibrinogen alpha chain
PRO_0000009038Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi48 – 48Interchain By similarity
Disulfide bondi56 – 56Interchain (with beta chain) By similarity
Disulfide bondi65 – 65Interchain (with C-49 in gamma chain) By similarity
Disulfide bondi69 – 69Interchain (with beta chain) By similarity
Disulfide bondi181 – 181Interchain (with C-165 in gamma chain) By similarity
Disulfide bondi185 – 185Interchain (with beta chain) By similarity
Disulfide bondi404 ↔ 434 By similarity
Glycosylationi602 – 6021N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The alpha chain is not glycosylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
Phosphorylation sites are present in the extracellular medium By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP06399.
PRIDEiP06399.

Miscellaneous databases

PMAP-CutDBP06399.

Expressioni

Gene expression databases

GenevestigatoriP06399.

Interactioni

Subunit structurei

Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain By similarity.

Structurei

3D structure databases

ProteinModelPortaliP06399.
SMRiP06399. Positions 46-93, 131-215, 586-782.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini539 – 780242Fibrinogen C-terminal
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili68 – 547480 By similarity
Add
BLAST

Domaini

A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiNOG114889.
HOGENOMiHOG000285947.
HOVERGENiHBG005668.
InParanoidiP06399.
PhylomeDBiP06399.

Family and domain databases

Gene3Di3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProiIPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR012290. Fibrinogen_a/b/g_coil_dom.
IPR021996. Fibrinogen_aC.
IPR020837. Fibrinogen_CS.
[Graphical view]
PfamiPF08702. Fib_alpha. 1 hit.
PF12160. Fibrinogen_aC. 1 hit.
PF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P06399-1) [UniParc]FASTAAdd to Basket

Also known as: Alpha-E

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLSLRVACLI LSLASTVWTA DTGTTSEFIE AGGDIRGPRI VERQPSQCKE    50
TDWPFCSDED WNHKCPSGCR MKGLIDEANQ DFTNRINKLK NSLFDFQKNN 100
KDSNSLTRNI MEYLRGDFAN ANNFDNTFGQ VSEDLRRRIQ ILKRKVIEKA 150
QQIQVLQKDV RDQLIDMKRL EVDIDIKIRS CKGSCSRSVS REINLKDYEG 200
QQKQLEQVIA KDLLPAKDRQ YLPAIKMSPV PDLVPGSFKS QLQEGPPEWK 250
ALTEMRQMRM ELERPGKDGA SRGDLPGDSR GDSATRGPGS KIENPMTPGH 300
GGSGYWRPGS SGSGSDGNWG SGTTGSDDTG TWGAGSSRPS SGSGNLKPSN 350
PDWGEFSEFG GSSSPATRKE YHTGKLVTSK GDKELLIGNE KVTSTGTSTT 400
RRSCSKTITK TVLGNDGHRE VVKEVVTSDD GSDCGDGMDL GLTHSFSGRL 450
DELSRMHPEL GSFYDSRFGS LTSNFKEFGS KTSDSDIFTD IENPSSHVPE 500
FSSSSKTSTV RKQVTKSYKM ADEAASEAHQ EGDTRTTKRG RARTMRDCDD 550
VLQTHPSGAQ NGIFSIKLPG SSKIFSVYCD QETSLGGWLL IQQRMDGSLN 600
FNRTWQDYKR GFGSLNDKGE GEFWLGNDYL HLLTLRGSVL RVELEDWAGK 650
EAYAEYHFRV GSEAEGYALQ VSSYQGTAGD ALMEGSVEEG TEYTSHSNMQ 700
FSTFDRDADQ WEENCAEVYG GGWWYNSCQA ANLNGIYYPG GTYDPRNNSP 750
YEIENGVLWV PFRGADYSLW AVRMKIRPLV GQ 782
Length:782
Mass (Da):86,686
Last modified:October 1, 1996 - v3
Checksum:i744834DAE76D34C2
GO
Isoform 2 (identifier: P06399-2) [UniParc]FASTAAdd to Basket

Also known as: Alpha

The sequence of this isoform differs from the canonical sequence as follows:
     547-550: DCDD → GIHA
     551-782: Missing.

Note: Major isoform.

Show »
Length:550
Mass (Da):60,490
Checksum:i24C2E1470DD2C7BF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei547 – 5504DCDD → GIHA in isoform 2.
VSP_001533
Alternative sequencei551 – 782232Missing in isoform 2.
VSP_001534Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 345EAGGD → DEGAG AA sequence 1 Publication
Sequence conflicti140 – 1401Q → E no nucleotide entry 1 Publication
Sequence conflicti212 – 2121D → E no nucleotide entry 1 Publication
Sequence conflicti270 – 2767ASRGDLP → LREEIYQ1 Publication
Sequence conflicti473 – 4731S → K in AAA41158. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X86561 Genomic DNA. Translation: CAA60264.1.
X86561 Genomic DNA. Translation: CAA60263.1.
M35601 mRNA. Translation: AAA41158.1.
PIRiI53408.
UniGeneiRn.98846.

Genome annotation databases

UCSCiRGD:2603. rat. [P06399-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X86561 Genomic DNA. Translation: CAA60264.1 .
X86561 Genomic DNA. Translation: CAA60263.1 .
M35601 mRNA. Translation: AAA41158.1 .
PIRi I53408.
UniGenei Rn.98846.

3D structure databases

ProteinModelPortali P06399.
SMRi P06399. Positions 46-93, 131-215, 586-782.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P06399.
PRIDEi P06399.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2603. rat. [P06399-1 ]

Organism-specific databases

RGDi 2603. Fga.

Phylogenomic databases

eggNOGi NOG114889.
HOGENOMi HOG000285947.
HOVERGENi HBG005668.
InParanoidi P06399.
PhylomeDBi P06399.

Miscellaneous databases

PMAP-CutDB P06399.
PROi P06399.

Gene expression databases

Genevestigatori P06399.

Family and domain databases

Gene3Di 3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProi IPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR012290. Fibrinogen_a/b/g_coil_dom.
IPR021996. Fibrinogen_aC.
IPR020837. Fibrinogen_CS.
[Graphical view ]
Pfami PF08702. Fib_alpha. 1 hit.
PF12160. Fibrinogen_aC. 1 hit.
PF00147. Fibrinogen_C. 1 hit.
[Graphical view ]
SMARTi SM00186. FBG. 1 hit.
[Graphical view ]
SUPFAMi SSF56496. SSF56496. 1 hit.
PROSITEi PS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Fibrinogen alpha genes: conservation of bipartite transcripts and carboxy-terminal-extended alpha subunits in vertebrates."
    Fu Y., Cao Y., Hertzberg K.M., Grieninger G.
    Genomics 30:71-76(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Evolution and structure of the fibrinogen genes. Random insertion of introns or selective loss?"
    Crabtree G.R., Comeau C.M., Fowlkes D.M., Fornace A.J. Jr., Malley J.D., Kant J.A.
    J. Mol. Biol. 185:1-19(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
  3. "Studies on fibrinopeptides from mammals."
    Blombaeck B., Blombaeck M., Grondahl N.J.
    Acta Chem. Scand. 19:1789-1791(1965)
    Cited for: PROTEIN SEQUENCE OF 20-36.
  4. "Molecular cloning of mRNA sequences transiently induced during rat liver regeneration."
    Sobczak J., Lotti A.-M., Taroux P., Duguet M.
    Exp. Cell Res. 169:47-56(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 458-550 (ISOFORM 2).
    Strain: Wistar.
    Tissue: Liver.

Entry informationi

Entry nameiFIBA_RAT
AccessioniPrimary (citable) accession number: P06399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi