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Reviewed, UniProtKB/Swiss-Prot P06367 (RS14A_YEAST)

Last modified June 16, 2009. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    40S ribosomal protein S14-A
Alternative name(s):
    RP59A
Gene names
Name: RPS14A
Synonyms: CRY1, RPL59
Ordered Locus Names: YCR031C
ORF Names: YCR31C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length137 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Ref.8

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.8 Ref.5

Subcellular location

Cytoplasm By similarity. Nucleusnucleolus.

Post-translational modification

N-terminally acetylated by acetyltransferase NatA.

Miscellaneous

Present with 29600 molecules/cell in log phase SD medium. Ref.7

There are 2 genes for S14 in yeast.

Sequence similarities

Belongs to the ribosomal protein S11P family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 13713640S ribosomal protein S14-A
PRO_0000123359

Amino acid modifications

Modified residue21N-acetylserine Ref.6 Ref.4 Ref.9
Modified residue21Phosphoserine Ref.9
Modified residue111Phosphoserine Ref.10 Ref.11
Modified residue1251Phosphoserine Ref.11

Experimental info

Sequence conflict721K → R in AAA34530. Ref.1
Sequence conflict1231S → C in AAA34530. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P06367-1 [UniParc].

Last modified January 23, 2007. Version 5.
Checksum: 65A9212E10340A95

FASTA13714,537
        10         20         30         40         50         60 
MSNVVQARDN SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA 

        70         80         90        100        110        120 
MLAAQDVAAK CKEVGITAVH VKIRATGGTR TKTPGPGGQA ALRALARSGL RIGRIEDVTP 

       130 
VPSDSTRKKG GRRGRRL 

« Hide

References

« Hide 'large scale' references
[1]"Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene."
Larkin J.C., Thompson J.R., Woolford J.L. Jr.
Mol. Cell. Biol. 7:1764-1775(1987) [PubMed: 3037334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed: 1574125] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 72 AND 123.
[4]"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
J. Biol. Chem. 267:5442-5445(1992) [PubMed: 1544921] [Abstract]
Cited for: ACETYLATION AT SER-2 BY NATA.
[5]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[6]"The action of N-terminal acetyltransferases on yeast ribosomal proteins."
Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract]
Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The small-subunit processome is a ribosome assembly intermediate."
Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J.
Eukaryot. Cell 3:1619-1626(2004) [PubMed: 15590835] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
[9]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION AT SER-2, MASS SPECTROMETRY.
[10]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, MASS SPECTROMETRY.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 AND SER-125, MASS SPECTROMETRY.
[12]"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
Cell 107:373-386(2001) [PubMed: 11701127] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 9-133, ELECTRON MICROSCOPY.
[13]"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract]
Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+Additional computationally mapped references.

Cross-references

Sequence databases

M16126 Genomic DNA. Translation: AAA34530.1.
X59720 Genomic DNA. Translation: CAC42981.1.
PIRR5BY59. A02726.
RefSeqNP_009960.2.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-K9-133[»]
1S1Helectron microscopy11.70K2-137[»]
SMRP06367. Positions 9-133.
ModBaseSearch...

Protein-protein interaction databases

IntActP06367. 36 interactions.

Proteomic databases

PRIDEP06367.

Genome annotation databases

EnsemblYCR031C. Saccharomyces cerevisiae. [Contig view]
GeneID850397.
GenomeReviewsGene locus YCR031C in contig X59720_GR.
KEGGsce:YCR031C.
NMPDRfig|4932.3.peg.685.

Organism-specific databases

CYGDYCR031c.
SGDS000000627. RPS14A.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP06367.
OMAP06367. IKVRAPG.

Gene expression databases

GermOnlineYCR031C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001971. Ribosomal_S11.
IPR018102. Ribosomal_S11_CS.
[Graphical view]
Gene3DG3DSA:3.30.420.80. Ribosomal_S11. 1 hit.
PANTHERPTHR11759. Ribosomal_S11. 1 hit.
PfamPF00411. Ribosomal_S11. 1 hit.
[Graphical view]
PIRSFPIRSF002131. Ribosomal_S11. 1 hit.
ProDomPD001010. Ribosomal_S11. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00054. RIBOSOMAL_S11. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio965926.

Entry information

Entry nameRS14A_YEAST
AccessionPrimary (citable) accession number: P06367
Secondary accession number(s): Q96VG9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 98 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents