40S ribosomal protein S14-A - Saccharomyces cerevisiae (Baker's yeast)

Names and origin

Protein names

Recommended name:
40S ribosomal protein S14-A
Alternative name(s):
RP59A

Gene names

Name:	RPS14A
Synonyms:	CRY1, RPL59
Ordered Locus Names:	YCR031C
ORF Names:	YCR31C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Ref.8
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.8 Ref.5
Subcellular location	Cytoplasm By similarity. Nucleus › nucleolus.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	Present with 29600 molecules/cell in log phase SD medium. Ref.7 There are 2 genes for S14 in yeast.
Sequence similarities	Belongs to the ribosomal protein S11P family.

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Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm Nucleus
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD ribosomal small subunit assembly Inferred from mutant phenotype. Source: SGD translation Ref.5 Non-traceable author statement. Source: SGD
Cellular component	90S preribosome Inferred from direct assay. Source: SGD cytosolic small ribosomal subunit Ref.5 Non-traceable author statement. Source: SGD nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell small-subunit processome Ref.8 Inferred from direct assay. Source: SGD
Molecular function	mRNA binding Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct structural constituent of ribosome Ref.5 Non-traceable author statement. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
	P43582	1	EBI-14460,EBI-22766
ESS1	P22696	1	EBI-14460,EBI-6679
PRP40	P33203	1	EBI-14460,EBI-701
SET2	P46995	1	EBI-14460,EBI-16985
URN1	Q06525	1	EBI-14460,EBI-35138

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed Ref.6

Chain

2 – 137

136

40S ribosomal protein S14-A

PRO_0000123359

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.6 Ref.4 Ref.9

Modified residue

2

1

Phosphoserine Ref.9

Modified residue

11

1

Phosphoserine Ref.10 Ref.11

Modified residue

125

1

Phosphoserine Ref.11

Experimental info

Sequence conflict

72

1

K → R in AAA34530. Ref.1

Sequence conflict

123

1

S → C in AAA34530. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P06367-1 [UniParc].

Last modified January 23, 2007. Version 5.
Checksum: 65A9212E10340A95
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FASTA

137

14,537

        10         20         30         40         50         60 
MSNVVQARDN SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA 

        70         80         90        100        110        120 
MLAAQDVAAK CKEVGITAVH VKIRATGGTR TKTPGPGGQA ALRALARSGL RIGRIEDVTP 

       130 
VPSDSTRKKG GRRGRRL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene." Larkin J.C., Thompson J.R., Woolford J.L. Jr. Mol. Cell. Biol. 7:1764-1775(1987) [PubMed: 3037334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Valles G., Volckaerts G. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 72 AND 123.
[4]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed: 1544921] [Abstract] Cited for: ACETYLATION AT SER-2 BY NATA.
[5]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[6]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The small-subunit processome is a ribosome assembly intermediate." Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J. Eukaryot. Cell 3:1619-1626(2004) [PubMed: 15590835] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION AT SER-2, MASS SPECTROMETRY.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 AND SER-125, MASS SPECTROMETRY.
[12]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 9-133, ELECTRON MICROSCOPY.
[13]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M16126 Genomic DNA. Translation: AAA34530.1.
X59720 Genomic DNA. Translation: CAC42981.1.

PIR

R5BY59. A02726.

RefSeq

NP_009960.2.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	K	9-133	[»]
1S1H	electron microscopy	11.70	K	2-137	[»]

SMR

P06367. Positions 9-133.

ModBase

Search...

Protein-protein interaction databases

IntAct

P06367. 36 interactions.

Proteomic databases

PRIDE

P06367.

Genome annotation databases

Ensembl

YCR031C. Saccharomyces cerevisiae. [Contig view]

GeneID

850397.

GenomeReviews

Gene locus YCR031C in contig X59720_GR.

KEGG

sce:YCR031C.

NMPDR

fig|4932.3.peg.685.

Organism-specific databases

CYGD

YCR031c.

SGD

S000000627. RPS14A.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P06367.

OMA

P06367. IKVRAPG.

Gene expression databases

GermOnline

YCR031C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001971. Ribosomal_S11.
IPR018102. Ribosomal_S11_CS.
[Graphical view]

Gene3D

G3DSA:3.30.420.80. Ribosomal_S11. 1 hit.

PANTHER

PTHR11759. Ribosomal_S11. 1 hit.

Pfam

PF00411. Ribosomal_S11. 1 hit.
[Graphical view]

PIRSF

PIRSF002131. Ribosomal_S11. 1 hit.

ProDom

PD001010. Ribosomal_S11. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00054. RIBOSOMAL_S11. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

965926.

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Entry information

Entry name

RS14A_YEAST

Accession

Primary (citable) accession number: P06367
Secondary accession number(s): Q96VG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 98 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents