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Protein

40S ribosomal protein S14-A

Gene

RPS14A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:22096102). uS11 is involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (PubMed:15590835).1 Publication1 Publication

Miscellaneous

Present with 29600 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for uS11 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit assembly Source: SGD
  • rRNA methylation Source: Reactome
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29345-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S14-A1 Publication
Alternative name(s):
RP59A
Small ribosomal subunit protein uS11-A1 Publication
Gene namesi
Name:RPS14A1 Publication
Synonyms:CRY1, RPL59
Ordered Locus Names:YCR031C
ORF Names:YCR31C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR031C.
SGDiS000000627. RPS14A.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001233592 – 13740S ribosomal protein S14-AAdd BLAST136

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources2 Publications1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP06367.
PRIDEiP06367.
TopDownProteomicsiP06367.

PTM databases

iPTMnetiP06367.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uS11 interacts with eS1 forming part of the mRNA exit tunnel (PubMed:9559554, PubMed:22096102). uS11 interacts with snoRNA U3. uS11 interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3 (PubMed:15590835).1 Publication2 Publications

Protein-protein interaction databases

BioGridi31014. 215 interactors.
IntActiP06367. 27 interactors.
MINTiMINT-4083975.
STRINGi4932.YCR031C.

Structurei

Secondary structure

1137
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 16Combined sources3
Beta strandi19 – 22Combined sources4
Beta strandi25 – 28Combined sources4
Beta strandi30 – 32Combined sources3
Beta strandi35 – 39Combined sources5
Turni44 – 46Combined sources3
Beta strandi50 – 52Combined sources3
Beta strandi54 – 56Combined sources3
Helixi57 – 74Combined sources18
Beta strandi78 – 80Combined sources3
Beta strandi82 – 84Combined sources3
Beta strandi88 – 90Combined sources3
Helixi96 – 98Combined sources3
Helixi99 – 106Combined sources8
Turni107 – 109Combined sources3
Beta strandi111 – 117Combined sources7
Turni131 – 133Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-K9-133[»]
3J6Xelectron microscopy6.10141-137[»]
3J6Yelectron microscopy6.10141-137[»]
3J77electron microscopy6.20141-137[»]
3J78electron microscopy6.30141-137[»]
4U3MX-ray3.00C4/c42-137[»]
4U3NX-ray3.20C4/c42-137[»]
4U3UX-ray2.90C4/c42-137[»]
4U4NX-ray3.10C4/c42-137[»]
4U4OX-ray3.60C4/c42-137[»]
4U4QX-ray3.00C4/c42-137[»]
4U4RX-ray2.80C4/c42-137[»]
4U4UX-ray3.00C4/c42-137[»]
4U4YX-ray3.20C4/c42-137[»]
4U4ZX-ray3.10C4/c42-137[»]
4U50X-ray3.20C4/c42-137[»]
4U51X-ray3.20C4/c42-137[»]
4U52X-ray3.00C4/c42-137[»]
4U53X-ray3.30C4/c42-137[»]
4U55X-ray3.20C4/c42-137[»]
4U56X-ray3.45C4/c42-137[»]
4U6FX-ray3.10C4/c42-137[»]
4V4Belectron microscopy11.70AK2-137[»]
4V6Ielectron microscopy8.80AK1-137[»]
4V7RX-ray4.00AH/CH1-137[»]
4V88X-ray3.00AO/CO1-137[»]
4V8Yelectron microscopy4.30AO1-137[»]
4V8Zelectron microscopy6.60AO1-137[»]
4V92electron microscopy3.70O11-137[»]
5DATX-ray3.15C4/c42-137[»]
5DC3X-ray3.25C4/c42-137[»]
5DGEX-ray3.45C4/c42-137[»]
5DGFX-ray3.30C4/c42-137[»]
5DGVX-ray3.10C4/c42-137[»]
5FCIX-ray3.40C4/c42-137[»]
5FCJX-ray3.10C4/c42-137[»]
5JPQelectron microscopy7.30w1-137[»]
5JUOelectron microscopy4.00LB1-137[»]
5JUPelectron microscopy3.50LB1-137[»]
5JUSelectron microscopy4.20LB1-137[»]
5JUTelectron microscopy4.00LB1-137[»]
5JUUelectron microscopy4.00LB1-137[»]
5LL6electron microscopy3.90Z1-137[»]
5LYBX-ray3.25C4/c410-137[»]
5M1Jelectron microscopy3.30O211-137[»]
5MC6electron microscopy3.80Z1-137[»]
5TGMX-ray3.50C4/c410-126[»]
5WLCelectron microscopy3.80NG1-137[»]
5WYJelectron microscopy8.70SP1-137[»]
5WYKelectron microscopy4.50SP1-137[»]
ProteinModelPortaliP06367.
SMRiP06367.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06367.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000000703.
HOGENOMiHOG000111598.
InParanoidiP06367.
KOiK02955.
OMAiVRRASHK.
OrthoDBiEOG092C5QK1.

Family and domain databases

Gene3Di3.30.420.80. 1 hit.
HAMAPiMF_01310. Ribosomal_S11. 1 hit.
InterProiView protein in InterPro
IPR001971. Ribosomal_S11.
IPR018102. Ribosomal_S11_CS.
IPR036967. Ribosomal_S11_sf.
PANTHERiPTHR11759. PTHR11759. 1 hit.
PfamiView protein in Pfam
PF00411. Ribosomal_S11. 1 hit.
PIRSFiPIRSF002131. Ribosomal_S11. 1 hit.
PROSITEiView protein in PROSITE
PS00054. RIBOSOMAL_S11. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVVQARDN SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA
60 70 80 90 100
DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGTR TKTPGPGGQA
110 120 130
ALRALARSGL RIGRIEDVTP VPSDSTRKKG GRRGRRL
Length:137
Mass (Da):14,537
Last modified:January 23, 2007 - v5
Checksum:i65A9212E10340A95
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72K → R in AAA34530 (PubMed:3037334).Curated1
Sequence conflicti123S → C in AAA34530 (PubMed:3037334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16126 Genomic DNA. Translation: AAA34530.1.
X59720 Genomic DNA. Translation: CAC42981.1.
BK006937 Genomic DNA. Translation: DAA07510.1.
PIRiA02726. R5BY59.
RefSeqiNP_009960.2. NM_001178745.1.

Genome annotation databases

EnsemblFungiiCAC42981; CAC42981; CAC42981.
YCR031C; YCR031C; YCR031C.
GeneIDi850397.
KEGGisce:YCR031C.

Similar proteinsi

Entry informationi

Entry nameiRS14A_YEAST
AccessioniPrimary (citable) accession number: P06367
Secondary accession number(s): D6VR41, Q96VG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 175 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names