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Protein

Histone H1t

Gene

Hist1h1t

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Testis-specific histone H1 that forms less compacted chromatin compared to other H1 histone subtypes. Formation of more relaxed chromatin may be required to promote chromatin architecture required for proper chromosome regulation during meiosis, such as homologous recombination. Histones H1 act as linkers that bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei54Important for nucleosome binding properties1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H1t
Gene namesi
Name:Hist1h1t
Synonyms:H1ft, H1t
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi2778. Hist1h1t.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54Q → K: Increased affinity for DNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001959252 – 208Histone H1tAdd BLAST207

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9Phosphoserine1 Publication1
Modified residuei56CitrullineBy similarity1
Modified residuei141Phosphoserine1 Publication1
Modified residuei156Phosphothreonine1 Publication1
Modified residuei163PhosphoserineBy similarity1
Modified residuei178Phosphoserine1 Publication1
Modified residuei187Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated in early spermatids.1 Publication
Citrullination at Arg-56 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.By similarity

Keywords - PTMi

Citrullination, Phosphoprotein

Proteomic databases

PRIDEiP06349.

PTM databases

iPTMnetiP06349.
PhosphoSitePlusiP06349.

Expressioni

Tissue specificityi

Testis-specific (PubMed:2423516, PubMed:1706721). Expressed in pachytene spermatocytes during meiotic prophase I (PubMed:1706721).2 Publications

Developmental stagei

This histone is a testis-specific H1 variant that appears during meiosis in spermatogenesis.1 Publication

Gene expression databases

GenevisibleiP06349. RN.

Interactioni

Protein-protein interaction databases

BioGridi246603. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP06349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 111H15PROSITE-ProRule annotationAdd BLAST74

Domaini

The Gln-54 residue in the globular domain forms a hydrogen bond with the main chain carbonyl of Met-52 and is spatially oriented away from the nucleosome dyad axis, leading to lower affinity towards Four-way junction DNA and reconstituted 5S mononucleosomes.1 Publication

Sequence similaritiesi

Belongs to the histone H1/H5 family.PROSITE-ProRule annotation
Contains 1 H15 (linker histone H1/H5 globular) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00670000097781.
HOGENOMiHOG000251627.
HOVERGENiHBG009035.
InParanoidiP06349.
KOiK11275.
OMAiMQKTDLR.
OrthoDBiEOG091G0XGD.
PhylomeDBiP06349.
TreeFamiTF313664.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005818. Histone_H1/H5_H15.
IPR005819. Histone_H5.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00538. Linker_histone. 1 hit.
[Graphical view]
PRINTSiPR00624. HISTONEH5.
SMARTiSM00526. H15. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS51504. H15. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06349-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETAPAASS TLVPAPVEKP ATKRRGKKPG MATARKPRGF SVSKLIPEAL
60 70 80 90 100
SMSQERAGMS LAALKKALAA AGYDVEKNNS RIKLALKRLV NKGVLVQTKG
110 120 130 140 150
TGASGSFKLS KKAASGNDKG KGKKSASAKA KKLGLSRASR SPKSSKTKVV
160 170 180 190 200
KKPKATPTKG SGSRRKTKGA KGLQQRKSPA KARATNSNSG KSKMVMQKTD

LRKAAGRK
Length:208
Mass (Da):21,726
Last modified:January 23, 2007 - v2
Checksum:iFAB7DAA110D850CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13170 Genomic DNA. Translation: AAA41328.1.
M28409 Genomic DNA. Translation: AAA41305.1.
PIRiA27779. HSRT1T.
RefSeqiNP_036711.1. NM_012579.1.
UniGeneiRn.138104.

Genome annotation databases

EnsembliENSRNOT00000081345; ENSRNOP00000070024; ENSRNOG00000061863.
GeneIDi24438.
KEGGirno:24438.
UCSCiRGD:2778. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13170 Genomic DNA. Translation: AAA41328.1.
M28409 Genomic DNA. Translation: AAA41305.1.
PIRiA27779. HSRT1T.
RefSeqiNP_036711.1. NM_012579.1.
UniGeneiRn.138104.

3D structure databases

ProteinModelPortaliP06349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246603. 2 interactors.

PTM databases

iPTMnetiP06349.
PhosphoSitePlusiP06349.

Proteomic databases

PRIDEiP06349.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000081345; ENSRNOP00000070024; ENSRNOG00000061863.
GeneIDi24438.
KEGGirno:24438.
UCSCiRGD:2778. rat.

Organism-specific databases

CTDi3010.
RGDi2778. Hist1h1t.

Phylogenomic databases

GeneTreeiENSGT00670000097781.
HOGENOMiHOG000251627.
HOVERGENiHBG009035.
InParanoidiP06349.
KOiK11275.
OMAiMQKTDLR.
OrthoDBiEOG091G0XGD.
PhylomeDBiP06349.
TreeFamiTF313664.

Miscellaneous databases

PROiP06349.

Gene expression databases

GenevisibleiP06349. RN.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005818. Histone_H1/H5_H15.
IPR005819. Histone_H5.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00538. Linker_histone. 1 hit.
[Graphical view]
PRINTSiPR00624. HISTONEH5.
SMARTiSM00526. H15. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS51504. H15. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiH1T_RAT
AccessioniPrimary (citable) accession number: P06349
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.