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P06340 (DOA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HLA class II histocompatibility antigen, DO alpha chain
Alternative name(s):
MHC DN-alpha
MHC DZ alpha
MHC class II antigen DOA
Gene names
Name:HLA-DOA
Synonyms:HLA-DNA, HLA-DZA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.

Subunit structure

Heterodimer of an alpha chain (DOA) and a beta chain (DOB). Forms a heterotetrameric complex with an HLA-DM molecule during intracellular transport in endosomal/lysosomal compartments in B-cells.

Subcellular location

Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER.

Polymorphism

The following alleles of DOA are known: DOA*01:01, DOA*01:02 and DOA*01:03. The sequence shown is that of DOA*01:01.

Sequence similarities

Belongs to the MHC class II family.

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 250225HLA class II histocompatibility antigen, DO alpha chain
PRO_0000018962

Regions

Topological domain26 – 217192Extracellular Potential
Transmembrane218 – 24023Helical; Potential
Topological domain241 – 25010Cytoplasmic Potential
Domain113 – 20593Ig-like C1-type
Region26 – 11085Alpha-1
Region111 – 20494Alpha-2
Region205 – 21713Connecting peptide

Amino acid modifications

Glycosylation1041N-linked (GlcNAc...) Potential
Glycosylation1441N-linked (GlcNAc...) Potential
Disulfide bond133 ↔ 189 By similarity

Natural variations

Natural variant991L → V in allele DOA*01:03.
Corresponds to variant rs41542323 [ dbSNP | Ensembl ].
VAR_058126
Natural variant1051R → C in allele DOA*01:02.
Corresponds to variant rs11575906 [ dbSNP | Ensembl ].
VAR_058127

Secondary structure

................................. 250
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06340 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: FA9482197A02AC85

FASTA25027,599
        10         20         30         40         50         60 
MALRAGLVLG FHTLMTLLSP QEAGATKADH MGSYGPAFYQ SYGASGQFTH EFDEEQLFSV 

        70         80         90        100        110        120 
DLKKSEAVWR LPEFGDFARF DPQGGLAGIA AIKAHLDILV ERSNRSRAIN VPPRVTVLPK 

       130        140        150        160        170        180 
SRVELGQPNI LICIVDNIFP PVINITWLRN GQTVTEGVAQ TSFYSQPDHL FRKFHYLPFV 

       190        200        210        220        230        240 
PSAEDVYDCQ VEHWGLDAPL LRHWELQVPI PPPDAMETLV CALGLAIGLV GFLVGTVLII 

       250 
MGTYVSSVPR 

« Hide

References

« Hide 'large scale' references
[1]"The human HLA class II alpha chain gene DZ alpha is distinct from genes in the DP, DQ and DR subregions."
Trowsdale J., Kelly A.
EMBO J. 4:2231-2237(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE DOA*01:01).
[2]"Human class II DNA and DOB genes display low sequence variability."
Jonsson A.-K., Rask L.
Immunogenetics 29:411-413(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE DOA*01:01).
[3]"The HLA-DNA (DZA) gene is correctly expressed as a 1.1 kb mature mRNA transcript."
Young J.A., Trowsdale J.
Immunogenetics 31:386-388(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE DOA*01:01).
[4]"Identification of four novel HLA-DOA alleles, DOA*010106, DOA*0102, DOA*0103, and DOA*0104N, by sequence-based typing."
Moon S.-M., Gu H., Ryu H.-J., Kim J.-J., Kim H.-T., Han B.G., Kimm K., Lee J.-K., Oh B.
Tissue Antigens 66:242-245(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES DOA*01:01; DOA*01:02 AND DOA*01:03).
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE DOA*01:01).
Tissue: Lung.
[6]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELE DOA*01:01).
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELE DOA*01:01).
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE DOA*01:01).
Tissue: Lymph.
[9]"Limited polymorphism in the HLA-DOA gene."
Naruse T.K., Kawata H., Anzai T., Takashige N., Kagiya M., Nose Y., Nabeya N., Isshiki G., Tatsumi N., Inoko H.
Tissue Antigens 53:359-365(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 29-250 (ALLELE DOA*01:01).
[10]"Nonclassical MHC class II molecules."
Alfonso C., Karlsson L.
Annu. Rev. Immunol. 18:113-142(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02882 Genomic DNA. Translation: CAA26635.1.
M26039 mRNA. Translation: AAA59716.1.
M31525 mRNA. Translation: AAA60075.1.
AY947479 mRNA. Translation: AAX49509.1.
AY947480 mRNA. Translation: AAX49510.1.
AY947481 mRNA. Translation: AAX49511.1.
AK290714 mRNA. Translation: BAF83403.1.
AK313551 mRNA. Translation: BAG36327.1.
Z81310 Genomic DNA. Translation: CAB03594.1.
AL662845 Genomic DNA. Translation: CAI17494.1.
AL645931 Genomic DNA. Translation: CAI18036.1.
AL805913 Genomic DNA. Translation: CAI18546.1.
BX005422 Genomic DNA. Translation: CAM26122.1.
CR759829 Genomic DNA. Translation: CAP58470.1.
CR936909 Genomic DNA. Translation: CAQ07150.1.
CR759795 Genomic DNA. Translation: CAQ07430.1.
CH471081 Genomic DNA. Translation: EAX03668.1.
BC013183 mRNA. Translation: AAH13183.1.
AB005991 Genomic DNA. Translation: BAA81787.1.
AB005992 Genomic DNA. Translation: BAA81788.1.
AB005993 Genomic DNA. Translation: BAA81789.1.
AB005994 Genomic DNA. Translation: BAA81790.1.
AB005995 Genomic DNA. Translation: BAA81791.1.
AB005996 Genomic DNA. Translation: BAA81792.1.
AB005997 Genomic DNA. Translation: BAA81793.1.
AB005998 Genomic DNA. Translation: BAA81794.1.
PIRHLHUDZ. A02216.
RefSeqNP_002110.1. NM_002119.3.
UniGeneHs.631991.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4I0PX-ray3.20C/G27-207[»]
ProteinModelPortalP06340.
SMRP06340. Positions 27-207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109356. 1 interaction.
DIPDIP-60116N.
MINTMINT-6630142.

Polymorphism databases

DMDM122210.

Proteomic databases

PaxDbP06340.
PRIDEP06340.

Protocols and materials databases

DNASU3111.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000229829; ENSP00000229829; ENSG00000204252.
ENST00000383226; ENSP00000372713; ENSG00000206292.
ENST00000426070; ENSP00000401504; ENSG00000231558.
ENST00000426685; ENSP00000397945; ENSG00000232957.
ENST00000434335; ENSP00000416448; ENSG00000232962.
ENST00000438987; ENSP00000395737; ENSG00000230141.
ENST00000452598; ENSP00000398819; ENSG00000235744.
GeneID3111.
KEGGhsa:3111.
UCSCuc003ocr.3. human.

Organism-specific databases

CTD3111.
GeneCardsGC06M032971.
GC06Mj32894.
GC06Mk32949.
GC06Ml33125.
GC06Mm33005.
GC06Mn32900.
GC06Mo33111.
HGNCHGNC:4936. HLA-DOA.
HPAHPA045038.
MIM142930. gene.
neXtProtNX_P06340.
PharmGKBPA35060.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45645.
HOVERGENHBG006862.
InParanoidP06340.
KOK06752.
OMAKADHMGS.
PhylomeDBP06340.
TreeFamTF333797.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressP06340.
BgeeP06340.
GenevestigatorP06340.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR001003. MHC_II_a_N.
[Graphical view]
PfamPF07654. C1-set. 1 hit.
PF00993. MHC_II_alpha. 1 hit.
[Graphical view]
SMARTSM00407. IGc1. 1 hit.
SM00920. MHC_II_alpha. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiHLA-DOA.
GenomeRNAi3111.
NextBio12345.
PROP06340.
SOURCESearch...

Entry information

Entry nameDOA_HUMAN
AccessionPrimary (citable) accession number: P06340
Secondary accession number(s): Q58HU0 expand/collapse secondary AC list , Q58HU1, Q5STC7, Q9TQC6, Q9TQC7, Q9TQC8, Q9TQC9, Q9TQD0, Q9TQD1, Q9TQD2, Q9TQD3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM