P06340 (DOA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HLA class II histocompatibility antigen, DO alpha chain

Alternative name(s):
MHC DN-alpha
MHC DZ alpha
MHC class II antigen DOA

Gene names

Name:	HLA-DOA
Synonyms:	HLA-DNA, HLA-DZA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.
Subunit structure	Heterodimer of an alpha chain (DOA) and a beta chain (DOB). Forms a heterotetrameric complex with an HLA-DM molecule during intracellular transport in endosomal/lysosomal compartments in B-cells.
Subcellular location	Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER.
Polymorphism	The following alleles of DOA are known: DOA*01:01, DOA*01:02 and DOA*01:03. The sequence shown is that of DOA*01:01.
Sequence similarities	Belongs to the MHC class II family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Immunity
Cellular component	Endosome Lysosome MHC II Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II Traceable author statement. Source: Reactome immune response Inferred from electronic annotation. Source: InterPro regulation of T cell differentiation Inferred from electronic annotation. Source: Compara
Cellular_component	MHC class II protein complex Inferred from electronic annotation. Source: UniProtKB-KW endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal membrane Traceable author statement. Source: Reactome plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	MHC class II receptor activity Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Potential

Chain

26 – 250

225

HLA class II histocompatibility antigen, DO alpha chain

PRO_0000018962

Regions

Topological domain

26 – 217

192

Extracellular Potential

Transmembrane

218 – 240

23

Helical; Potential

Topological domain

241 – 250

10

Cytoplasmic Potential

Domain

113 – 205

93

Ig-like C1-type

Region

26 – 110

85

Alpha-1

Region

111 – 204

94

Alpha-2

Region

205 – 217

13

Connecting peptide

Amino acid modifications

Glycosylation

104

1

N-linked (GlcNAc...) Potential

Glycosylation

144

1

N-linked (GlcNAc...) Potential

Disulfide bond

133 ↔ 189

By similarity

Natural variations

Natural variant

99

1

L → V in allele DOA*01:03.
Corresponds to variant rs41542323 [ dbSNP | Ensembl ].

VAR_058126

Natural variant

105

1

R → C in allele DOA*01:02.
Corresponds to variant rs11575906 [ dbSNP | Ensembl ].

VAR_058127

Secondary structure

1

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250

Helix Strand Turn

Details...

Beta strand

29 – 40

12

Helix

42 – 44

3

Beta strand

45 – 52

8

Beta strand

55 – 61

7

Turn

62 – 65

4

Beta strand

66 – 71

6

Helix

72 – 74

3

Beta strand

77 – 80

4

Helix

87 – 94

8

Helix

96 – 102

7

Beta strand

114 – 121

8

Beta strand

129 – 141

13

Beta strand

144 – 149

6

Beta strand

152 – 154

3

Beta strand

171 – 179

9

Beta strand

187 – 192

6

Beta strand

196 – 198

3

Beta strand

200 – 204

5

Sequences

	Sequence		Length	Mass (Da)
	P06340 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: FA9482197A02AC85	FASTA	250	27,599
	10 20 30 40 50 60 MALRAGLVLG FHTLMTLLSP QEAGATKADH MGSYGPAFYQ SYGASGQFTH EFDEEQLFSV 70 80 90 100 110 120 DLKKSEAVWR LPEFGDFARF DPQGGLAGIA AIKAHLDILV ERSNRSRAIN VPPRVTVLPK 130 140 150 160 170 180 SRVELGQPNI LICIVDNIFP PVINITWLRN GQTVTEGVAQ TSFYSQPDHL FRKFHYLPFV 190 200 210 220 230 240 PSAEDVYDCQ VEHWGLDAPL LRHWELQVPI PPPDAMETLV CALGLAIGLV GFLVGTVLII 250 MGTYVSSVPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human HLA class II alpha chain gene DZ alpha is distinct from genes in the DP, DQ and DR subregions." Trowsdale J., Kelly A. EMBO J. 4:2231-2237(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE DOA*01:01).
[2]	"Human class II DNA and DOB genes display low sequence variability." Jonsson A.-K., Rask L. Immunogenetics 29:411-413(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE DOA*01:01).
[3]	"The HLA-DNA (DZA) gene is correctly expressed as a 1.1 kb mature mRNA transcript." Young J.A., Trowsdale J. Immunogenetics 31:386-388(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE DOA*01:01).
[4]	"Identification of four novel HLA-DOA alleles, DOA*010106, DOA*0102, DOA*0103, and DOA*0104N, by sequence-based typing." Moon S.-M., Gu H., Ryu H.-J., Kim J.-J., Kim H.-T., Han B.G., Kimm K., Lee J.-K., Oh B. Tissue Antigens 66:242-245(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES DOA*01:01; DOA*01:02 AND DOA*01:03).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE DOA*01:01). Tissue: Lung.
[6]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELE DOA*01:01).
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELE DOA*01:01).
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE DOA*01:01). Tissue: Lymph.
[9]	"Limited polymorphism in the HLA-DOA gene." Naruse T.K., Kawata H., Anzai T., Takashige N., Kagiya M., Nose Y., Nabeya N., Isshiki G., Tatsumi N., Inoko H. Tissue Antigens 53:359-365(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 29-250 (ALLELE DOA*01:01).
[10]	"Nonclassical MHC class II molecules." Alfonso C., Karlsson L. Annu. Rev. Immunol. 18:113-142(2000) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X02882 Genomic DNA. Translation: CAA26635.1.
M26039 mRNA. Translation: AAA59716.1.
M31525 mRNA. Translation: AAA60075.1.
AY947479 mRNA. Translation: AAX49509.1.
AY947480 mRNA. Translation: AAX49510.1.
AY947481 mRNA. Translation: AAX49511.1.
AK290714 mRNA. Translation: BAF83403.1.
AK313551 mRNA. Translation: BAG36327.1.
Z81310 Genomic DNA. Translation: CAB03594.1.
AL662845 Genomic DNA. Translation: CAI17494.1.
AL645931 Genomic DNA. Translation: CAI18036.1.
AL805913 Genomic DNA. Translation: CAI18546.1.
BX005422 Genomic DNA. Translation: CAM26122.1.
CR759829 Genomic DNA. Translation: CAP58470.1.
CR936909 Genomic DNA. Translation: CAQ07150.1.
CR759795 Genomic DNA. Translation: CAQ07430.1.
CH471081 Genomic DNA. Translation: EAX03668.1.
BC013183 mRNA. Translation: AAH13183.1.
AB005991 Genomic DNA. Translation: BAA81787.1.
AB005992 Genomic DNA. Translation: BAA81788.1.
AB005993 Genomic DNA. Translation: BAA81789.1.
AB005994 Genomic DNA. Translation: BAA81790.1.
AB005995 Genomic DNA. Translation: BAA81791.1.
AB005996 Genomic DNA. Translation: BAA81792.1.
AB005997 Genomic DNA. Translation: BAA81793.1.
AB005998 Genomic DNA. Translation: BAA81794.1.

IPI

IPI00026244.

PIR

HLHUDZ. A02216.

RefSeq

NP_002110.1. NM_002119.3.

UniGene

Hs.631991.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4I0P	X-ray	3.20	C/G	27-207	[»]

ProteinModelPortal

P06340.

ModBase

Search...

Polymorphism databases

DMDM

122210.

Proteomic databases

PaxDb

P06340.

PRIDE

P06340.

Protocols and materials databases

DNASU

3111.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000229829; ENSP00000229829; ENSG00000204252.
ENST00000383226; ENSP00000372713; ENSG00000206292.
ENST00000426070; ENSP00000401504; ENSG00000231558.
ENST00000426685; ENSP00000397945; ENSG00000232957.
ENST00000434335; ENSP00000416448; ENSG00000232962.
ENST00000438987; ENSP00000395737; ENSG00000230141.
ENST00000452598; ENSP00000398819; ENSG00000235744.

GeneID

3111.

KEGG

hsa:3111.

UCSC

uc003ocr.3. human.

Organism-specific databases

CTD

3111.

GeneCards

GC06M032971.

HGNC

HGNC:4936. HLA-DOA.

HPA

HPA045038.

MIM

142930. gene.

neXtProt

NX_P06340.

PharmGKB

PA35060.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG45645.

HOVERGEN

HBG006862.

InParanoid

P06340.

KO

K06752.

OMA

KADHMGS.

OrthoDB

EOG4KWJTP.

PhylomeDB

P06340.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

ArrayExpress

P06340.

Bgee

P06340.

Genevestigator

P06340.

GermOnline

ENSG00000204252. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.
3.10.320.10. 1 hit.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR001003. MHC_II_a_N.
[Graphical view]

Pfam

PF07654. C1-set. 1 hit.
PF00993. MHC_II_alpha. 1 hit.
[Graphical view]

SMART

SM00407. IGc1. 1 hit.
SM00920. MHC_II_alpha. 1 hit.
[Graphical view]

SUPFAM

SSF54452. MHC_I/II-like_Ag-recog. 1 hit.

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

3111.

NextBio

12345.

SOURCE

Search...

Entry information

Entry name

DOA_HUMAN

Accession

Primary (citable) accession number: P06340
Secondary accession number(s): Q58HU0 , Q58HU1, Q5STC7, Q9TQC6, Q9TQC7, Q9TQC8, Q9TQC9, Q9TQD0, Q9TQD1, Q9TQD2, Q9TQD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.