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Protein

HLA class II histocompatibility antigen, DO alpha chain

Gene

HLA-DOA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.

GO - Molecular functioni

  • MHC class II protein complex binding Source: UniProtKB
  • MHC class II receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity

Enzyme and pathway databases

BioCyciZFISH:ENSG00000112095-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.

Names & Taxonomyi

Protein namesi
Recommended name:
HLA class II histocompatibility antigen, DO alpha chain
Alternative name(s):
MHC DN-alpha
MHC DZ alpha
MHC class II antigen DOA
Gene namesi
Name:HLA-DOA
Synonyms:HLA-DNA, HLA-DZA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:4936. HLA-DOA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 217ExtracellularSequence analysisAdd BLAST192
Transmembranei218 – 240HelicalSequence analysisAdd BLAST23
Topological domaini241 – 250CytoplasmicSequence analysis10

GO - Cellular componenti

  • endosome membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • lysosomal membrane Source: Reactome
  • MHC class II protein complex Source: UniProtKB
  • plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane, MHC II

Pathology & Biotechi

Organism-specific databases

DisGeNETi3111.
OpenTargetsiENSG00000204252.
ENSG00000206292.
ENSG00000230141.
ENSG00000231558.
ENSG00000232957.
ENSG00000232962.
ENSG00000235744.
PharmGKBiPA35060.

Polymorphism and mutation databases

BioMutaiHLA-DOA.
DMDMi122210.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001896226 – 250HLA class II histocompatibility antigen, DO alpha chainAdd BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi104N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi133 ↔ 189PROSITE-ProRule annotation
Glycosylationi144N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP06340.
PaxDbiP06340.
PeptideAtlasiP06340.
PRIDEiP06340.

PTM databases

iPTMnetiP06340.
PhosphoSitePlusiP06340.

Expressioni

Gene expression databases

BgeeiENSG00000204252.
ExpressionAtlasiP06340. baseline and differential.
GenevisibleiP06340. HS.

Organism-specific databases

HPAiHPA045038.

Interactioni

Subunit structurei

Heterodimer of an alpha chain (DOA) and a beta chain (DOB). Forms a heterotetrameric complex with an HLA-DM molecule during intracellular transport in endosomal/lysosomal compartments in B-cells.

Binary interactionsi

WithEntry#Exp.IntActNotes
RELQ048643EBI-10194851,EBI-307352

GO - Molecular functioni

  • MHC class II protein complex binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109356. 3 interactors.
DIPiDIP-60116N.
IntActiP06340. 1 interactor.
MINTiMINT-6630142.
STRINGi9606.ENSP00000229829.

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 40Combined sources12
Helixi42 – 44Combined sources3
Beta strandi45 – 52Combined sources8
Beta strandi55 – 61Combined sources7
Turni62 – 65Combined sources4
Beta strandi66 – 71Combined sources6
Helixi72 – 74Combined sources3
Beta strandi77 – 80Combined sources4
Helixi87 – 94Combined sources8
Helixi96 – 102Combined sources7
Beta strandi114 – 121Combined sources8
Beta strandi129 – 141Combined sources13
Beta strandi144 – 149Combined sources6
Beta strandi152 – 154Combined sources3
Beta strandi171 – 179Combined sources9
Beta strandi187 – 192Combined sources6
Beta strandi196 – 198Combined sources3
Beta strandi200 – 204Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I0PX-ray3.20C/G27-207[»]
ProteinModelPortaliP06340.
SMRiP06340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini113 – 205Ig-like C1-typeAdd BLAST93

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 110Alpha-1Add BLAST85
Regioni111 – 204Alpha-2Add BLAST94
Regioni205 – 217Connecting peptideAdd BLAST13

Sequence similaritiesi

Belongs to the MHC class II family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IM01. Eukaryota.
ENOG4111C4S. LUCA.
GeneTreeiENSGT00760000118970.
HOVERGENiHBG006862.
InParanoidiP06340.
KOiK06752.
OMAiKADHMGS.
OrthoDBiEOG091G0IC9.
PhylomeDBiP06340.
TreeFamiTF333797.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR032431. C1-set_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR001003. MHC_II_a_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF16196. C1-set_C. 1 hit.
PF00993. MHC_II_alpha. 1 hit.
[Graphical view]
SMARTiSM00407. IGc1. 1 hit.
SM00920. MHC_II_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRAGLVLG FHTLMTLLSP QEAGATKADH MGSYGPAFYQ SYGASGQFTH
60 70 80 90 100
EFDEEQLFSV DLKKSEAVWR LPEFGDFARF DPQGGLAGIA AIKAHLDILV
110 120 130 140 150
ERSNRSRAIN VPPRVTVLPK SRVELGQPNI LICIVDNIFP PVINITWLRN
160 170 180 190 200
GQTVTEGVAQ TSFYSQPDHL FRKFHYLPFV PSAEDVYDCQ VEHWGLDAPL
210 220 230 240 250
LRHWELQVPI PPPDAMETLV CALGLAIGLV GFLVGTVLII MGTYVSSVPR
Length:250
Mass (Da):27,599
Last modified:January 1, 1988 - v1
Checksum:iFA9482197A02AC85
GO

Polymorphismi

The following alleles of DOA are known: DOA*01:01, DOA*01:02 and DOA*01:03. The sequence shown is that of DOA*01:01.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05812699L → V in allele DOA*01:03. Corresponds to variant rs41542323dbSNPEnsembl.1
Natural variantiVAR_058127105R → C in allele DOA*01:02. Corresponds to variant rs11575906dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02882 Genomic DNA. Translation: CAA26635.1.
M26039 mRNA. Translation: AAA59716.1.
M31525 mRNA. Translation: AAA60075.1.
AY947479 mRNA. Translation: AAX49509.1.
AY947480 mRNA. Translation: AAX49510.1.
AY947481 mRNA. Translation: AAX49511.1.
AK290714 mRNA. Translation: BAF83403.1.
AK313551 mRNA. Translation: BAG36327.1.
Z81310 Genomic DNA. Translation: CAB03594.1.
AL662845 Genomic DNA. Translation: CAI17494.1.
AL645931 Genomic DNA. Translation: CAI18036.1.
AL805913 Genomic DNA. Translation: CAI18546.1.
BX005422 Genomic DNA. Translation: CAM26122.1.
CR759829 Genomic DNA. Translation: CAP58470.1.
CR936909 Genomic DNA. Translation: CAQ07150.1.
CR759795 Genomic DNA. Translation: CAQ07430.1.
CH471081 Genomic DNA. Translation: EAX03668.1.
BC013183 mRNA. Translation: AAH13183.1.
AB005991 Genomic DNA. Translation: BAA81787.1.
AB005992 Genomic DNA. Translation: BAA81788.1.
AB005993 Genomic DNA. Translation: BAA81789.1.
AB005994 Genomic DNA. Translation: BAA81790.1.
AB005995 Genomic DNA. Translation: BAA81791.1.
AB005996 Genomic DNA. Translation: BAA81792.1.
AB005997 Genomic DNA. Translation: BAA81793.1.
AB005998 Genomic DNA. Translation: BAA81794.1.
CCDSiCCDS4763.1.
PIRiA02216. HLHUDZ.
RefSeqiNP_002110.1. NM_002119.3.
UniGeneiHs.631991.

Genome annotation databases

EnsembliENST00000229829; ENSP00000229829; ENSG00000204252.
ENST00000383226; ENSP00000372713; ENSG00000206292.
ENST00000426070; ENSP00000401504; ENSG00000231558.
ENST00000426685; ENSP00000397945; ENSG00000232957.
ENST00000434335; ENSP00000416448; ENSG00000232962.
ENST00000438987; ENSP00000395737; ENSG00000230141.
ENST00000452598; ENSP00000398819; ENSG00000235744.
GeneIDi3111.
KEGGihsa:3111.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02882 Genomic DNA. Translation: CAA26635.1.
M26039 mRNA. Translation: AAA59716.1.
M31525 mRNA. Translation: AAA60075.1.
AY947479 mRNA. Translation: AAX49509.1.
AY947480 mRNA. Translation: AAX49510.1.
AY947481 mRNA. Translation: AAX49511.1.
AK290714 mRNA. Translation: BAF83403.1.
AK313551 mRNA. Translation: BAG36327.1.
Z81310 Genomic DNA. Translation: CAB03594.1.
AL662845 Genomic DNA. Translation: CAI17494.1.
AL645931 Genomic DNA. Translation: CAI18036.1.
AL805913 Genomic DNA. Translation: CAI18546.1.
BX005422 Genomic DNA. Translation: CAM26122.1.
CR759829 Genomic DNA. Translation: CAP58470.1.
CR936909 Genomic DNA. Translation: CAQ07150.1.
CR759795 Genomic DNA. Translation: CAQ07430.1.
CH471081 Genomic DNA. Translation: EAX03668.1.
BC013183 mRNA. Translation: AAH13183.1.
AB005991 Genomic DNA. Translation: BAA81787.1.
AB005992 Genomic DNA. Translation: BAA81788.1.
AB005993 Genomic DNA. Translation: BAA81789.1.
AB005994 Genomic DNA. Translation: BAA81790.1.
AB005995 Genomic DNA. Translation: BAA81791.1.
AB005996 Genomic DNA. Translation: BAA81792.1.
AB005997 Genomic DNA. Translation: BAA81793.1.
AB005998 Genomic DNA. Translation: BAA81794.1.
CCDSiCCDS4763.1.
PIRiA02216. HLHUDZ.
RefSeqiNP_002110.1. NM_002119.3.
UniGeneiHs.631991.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I0PX-ray3.20C/G27-207[»]
ProteinModelPortaliP06340.
SMRiP06340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109356. 3 interactors.
DIPiDIP-60116N.
IntActiP06340. 1 interactor.
MINTiMINT-6630142.
STRINGi9606.ENSP00000229829.

PTM databases

iPTMnetiP06340.
PhosphoSitePlusiP06340.

Polymorphism and mutation databases

BioMutaiHLA-DOA.
DMDMi122210.

Proteomic databases

EPDiP06340.
PaxDbiP06340.
PeptideAtlasiP06340.
PRIDEiP06340.

Protocols and materials databases

DNASUi3111.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000229829; ENSP00000229829; ENSG00000204252.
ENST00000383226; ENSP00000372713; ENSG00000206292.
ENST00000426070; ENSP00000401504; ENSG00000231558.
ENST00000426685; ENSP00000397945; ENSG00000232957.
ENST00000434335; ENSP00000416448; ENSG00000232962.
ENST00000438987; ENSP00000395737; ENSG00000230141.
ENST00000452598; ENSP00000398819; ENSG00000235744.
GeneIDi3111.
KEGGihsa:3111.

Organism-specific databases

CTDi3111.
DisGeNETi3111.
GeneCardsiHLA-DOA.
HGNCiHGNC:4936. HLA-DOA.
HPAiHPA045038.
MIMi142930. gene.
neXtProtiNX_P06340.
OpenTargetsiENSG00000204252.
ENSG00000206292.
ENSG00000230141.
ENSG00000231558.
ENSG00000232957.
ENSG00000232962.
ENSG00000235744.
PharmGKBiPA35060.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IM01. Eukaryota.
ENOG4111C4S. LUCA.
GeneTreeiENSGT00760000118970.
HOVERGENiHBG006862.
InParanoidiP06340.
KOiK06752.
OMAiKADHMGS.
OrthoDBiEOG091G0IC9.
PhylomeDBiP06340.
TreeFamiTF333797.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000112095-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.

Miscellaneous databases

ChiTaRSiHLA-DOA. human.
GeneWikiiHLA-DOA.
GenomeRNAii3111.
PROiP06340.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204252.
ExpressionAtlasiP06340. baseline and differential.
GenevisibleiP06340. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR032431. C1-set_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR001003. MHC_II_a_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF16196. C1-set_C. 1 hit.
PF00993. MHC_II_alpha. 1 hit.
[Graphical view]
SMARTiSM00407. IGc1. 1 hit.
SM00920. MHC_II_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOA_HUMAN
AccessioniPrimary (citable) accession number: P06340
Secondary accession number(s): Q58HU0
, Q58HU1, Q5STC7, Q9TQC6, Q9TQC7, Q9TQC8, Q9TQC9, Q9TQD0, Q9TQD1, Q9TQD2, Q9TQD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.