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P06332 (CD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-cell surface glycoprotein CD4
Alternative name(s):
T-cell differentiation antigen L3T4
T-cell surface antigen T4/Leu-3
CD_antigen=CD4
Gene names
Name:Cd4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Subunit structure

Associates with p56-LCK. Interacts with SPG21 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processAdaptive immunity
Immunity
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processT cell differentiation

Inferred from sequence or structural similarity PubMed 1533274. Source: UniProtKB

T cell selection

Inferred from sequence or structural similarity PubMed 9551897. Source: UniProtKB

cell adhesion

Inferred from electronic annotation. Source: InterPro

cell surface receptor signaling pathway

Inferred from direct assay PubMed 9208839. Source: MGI

cytokine production

Inferred from mutant phenotype PubMed 7930593. Source: MGI

defense response to Gram-negative bacterium

Inferred from mutant phenotype PubMed 14688352. Source: MGI

immune response

Inferred from electronic annotation. Source: InterPro

induction by virus of host cell-cell fusion

Inferred from electronic annotation. Source: Ensembl

maintenance of protein location in cell

Inferred from sequence orthology PubMed 15128768. Source: MGI

positive regulation of T cell activation

Inferred from direct assay PubMed 12421911. Source: MGI

positive regulation of calcium-mediated signaling

Inferred from direct assay PubMed 9208839. Source: MGI

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 9208839. Source: MGI

positive regulation of protein kinase activity

Inferred from electronic annotation. Source: Ensembl

protein palmitoleylation

Inferred from sequence orthology PubMed 15128768. Source: MGI

   Cellular_componentcell surface

Inferred from direct assay PubMed 8612131. Source: MGI

endoplasmic reticulum lumen

Inferred from direct assay PubMed 16223484. Source: MGI

endoplasmic reticulum membrane

Inferred from direct assay PubMed 16223484. Source: MGI

external side of plasma membrane

Inferred from direct assay PubMed 10072077PubMed 10704459PubMed 10943842PubMed 12421911PubMed 14609575PubMed 15322158PubMed 15368291PubMed 15728238PubMed 15795238PubMed 16287714PubMed 16455951PubMed 16973387PubMed 17082577PubMed 17357106PubMed 18641307PubMed 18776904PubMed 18836449PubMed 19008373PubMed 19349303PubMed 19723499PubMed 19838199PubMed 19934022PubMed 20399120PubMed 20706986PubMed 20709950PubMed 21460847PubMed 21508411PubMed 22072979PubMed 7477352PubMed 7477353PubMed 7589135PubMed 7630421PubMed 7688139PubMed 8524870PubMed 8755570PubMed 8769481PubMed 8788039PubMed 9133426PubMed 9208839. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane raft

Inferred from direct assay PubMed 15128768. Source: MGI

plasma membrane

Inferred from direct assay PubMed 14517216. Source: MGI

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 24034254PubMed 7486703PubMed 7664803. Source: IntAct

zinc ion binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LckP062403EBI-1404,EBI-1401

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P06332-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P06332-2)

Also known as: Brain-specific;

The sequence of this isoform differs from the canonical sequence as follows:
     1-240: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Ref.7
Chain27 – 457431T-cell surface glycoprotein CD4
PRO_0000014627

Regions

Topological domain27 – 394368Extracellular Potential
Transmembrane395 – 41723Helical; Potential
Topological domain418 – 45740Cytoplasmic Potential
Domain27 – 128102Ig-like V-type
Domain129 – 20779Ig-like C2-type 1
Domain208 – 317110Ig-like C2-type 2
Domain318 – 37457Ig-like C2-type 3

Amino acid modifications

Lipidation4181S-palmitoyl cysteine By similarity
Lipidation4211S-palmitoyl cysteine By similarity
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation2981N-linked (GlcNAc...) Potential
Glycosylation3231N-linked (GlcNAc...) Potential
Glycosylation3921N-linked (GlcNAc...) Potential
Disulfide bond42 ↔ 112 Ref.8
Disulfide bond159 ↔ 188 Ref.8
Disulfide bond328 ↔ 370 Ref.8

Natural variations

Alternative sequence1 – 240240Missing in isoform 2.
VSP_002489

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 1B1DA7527CB00F33

FASTA45751,297
        10         20         30         40         50         60 
MCRAISLRRL LLLLLQLSQL LAVTQGKTLV LGKEGESAEL PCESSQKKIT VFTWKFSDQR 

        70         80         90        100        110        120 
KILGQHGKGV LIRGGSPSQF DRFDSKKGAW EKGSFPLIIN KLKMEDSQTY ICELENRKEE 

       130        140        150        160        170        180 
VELWVFKVTF SPGTSLLQGQ SLTLTLDSNS KVSNPLTECK HKKGKVVSGS KVLSMSNLRV 

       190        200        210        220        230        240 
QDSDFWNCTV TLDQKKNWFG MTLSVLGFQS TAITAYKSEG ESAEFSFPLN FAEENGWGEL 

       250        260        270        280        290        300 
MWKAEKDSFF QPWISFSIKN KEVSVQKSTK DLKLQLKETL PLTLKIPQVS LQFAGSGNLT 

       310        320        330        340        350        360 
LTLDKGTLHQ EVNLVVMKVA QLNNTLTCEV MGPTSPKMRL TLKQENQEAR VSEEQKVVQV 

       370        380        390        400        410        420 
VAPETGLWQC LLSEGDKVKM DSRIQVLSRG VNQTVFLACV LGGSFGFLGF LGLCILCCVR 

       430        440        450 
CRHQQRQAAR MSQIKRLLSE KKTCQCPHRM QKSHNLI 

« Hide

Isoform 2 (Brain-specific) [UniParc].

Checksum: 53589C44281646C5
Show »

FASTA21724,473

References

« Hide 'large scale' references
[1]"Isolation and sequence of L3T4 complementary DNA clones: expression in T cells and brain."
Tourvieille B., Gorman S.D., Field E.H., Hunkapiller T., Parnes J.R.
Science 234:610-614(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Unusual intron in the immunoglobulin domain of the newly isolated murine CD4 (L3T4) gene."
Littman D.R., Gettner S.N.
Nature 325:453-455(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"L3T4 and the immunoglobulin gene superfamily: new relationships between the immune system and the nervous system."
Parnes J.R., Hunkapiller T.
Immunol. Rev. 100:109-127(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Brain.
[4]"Structure of the mouse gene encoding CD4 and an unusual transcript in brain."
Gorman S.D., Tourvieille B., Parnes J.R.
Proc. Natl. Acad. Sci. U.S.A. 84:7644-7648(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
Tissue: Brain.
[5]"Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Mammary gland.
[7]"The L3T4 antigen in mouse and the sheep equivalent are immunoglobulin-like."
Classon B.J., Tsagaratos J., Kirszbaum L., Maddox J., McKay C.R., Brandon M., McKenzie I.F.C., Walker I.D.
Immunogenetics 23:129-132(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-43.
[8]"Partial primary structure of the T4 antigens of mouse and sheep: assignment of intrachain disulfide bonds."
Classon B.J., Tsagaratos J., McKenzie I.F.C., Walker I.D.
Proc. Natl. Acad. Sci. U.S.A. 83:4499-4503(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M36850 mRNA. Translation: AAA39401.1.
M13816 mRNA. Translation: AAA37267.1.
X04836 mRNA. Translation: CAA28539.1.
M36851 Genomic DNA. Translation: AAA39402.1.
M17080, M17078, M17079 Genomic DNA. Translation: AAA37403.1.
AC002397 Genomic DNA. Translation: AAC36010.1.
BC039137 mRNA. Translation: AAH39137.1.
CCDSCCDS20535.1. [P06332-1]
PIRRWMST4. A02110.
RefSeqNP_038516.1. NM_013488.2. [P06332-1]
UniGeneMm.2209.

3D structure databases

ProteinModelPortalP06332.
SMRP06332. Positions 27-387, 420-453.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198599. 1 interaction.
IntActP06332. 3 interactions.
MINTMINT-5024918.

PTM databases

PhosphoSiteP06332.

Proteomic databases

MaxQBP06332.
PRIDEP06332.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000024044; ENSMUSP00000024044; ENSMUSG00000023274. [P06332-1]
GeneID12504.
KEGGmmu:12504.
UCSCuc009dsi.1. mouse. [P06332-1]

Organism-specific databases

CTD920.
MGIMGI:88335. Cd4.

Phylogenomic databases

eggNOGNOG47205.
HOGENOMHOG000008696.
HOVERGENHBG005281.
InParanoidP06332.
KOK06454.
OMAPEAGMWQ.
OrthoDBEOG7HXCQS.
PhylomeDBP06332.
TreeFamTF335974.

Gene expression databases

ArrayExpressP06332.
BgeeP06332.
CleanExMM_CD4.
GenevestigatorP06332.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR000973. Ag_CD4.
IPR015274. CD4-extracel.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
IPR003596. Ig_V-set_subgr.
IPR013151. Immunoglobulin.
IPR021963. Tcell_CD4_Cterm.
[Graphical view]
PfamPF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF00047. ig. 1 hit.
PF12104. Tcell_CD4_Cterm. 1 hit.
[Graphical view]
PRINTSPR00692. CD4TCANTIGEN.
SMARTSM00409. IG. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281462.
PROP06332.
SOURCESearch...

Entry information

Entry nameCD4_MOUSE
AccessionPrimary (citable) accession number: P06332
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: July 9, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot