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P06332

- CD4_MOUSE

UniProt

P06332 - CD4_MOUSE

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Protein
T-cell surface glycoprotein CD4
Gene
Cd4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. zinc ion binding Source: Ensembl

GO - Biological processi

  1. T cell differentiation Source: UniProtKB
  2. T cell selection Source: UniProtKB
  3. cell adhesion Source: InterPro
  4. cell surface receptor signaling pathway Source: MGI
  5. cytokine production Source: MGI
  6. defense response to Gram-negative bacterium Source: MGI
  7. immune response Source: InterPro
  8. induction by virus of host cell-cell fusion Source: Ensembl
  9. maintenance of protein location in cell Source: MGI
  10. positive regulation of T cell activation Source: MGI
  11. positive regulation of calcium-mediated signaling Source: MGI
  12. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  13. positive regulation of protein kinase activity Source: Ensembl
  14. protein palmitoleylation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiREACT_215414. Translocation of ZAP-70 to Immunological synapse.
REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
REACT_225145. Downstream TCR signaling.
REACT_225768. Generation of second messenger molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD4
Alternative name(s):
T-cell differentiation antigen L3T4
T-cell surface antigen T4/Leu-3
CD_antigen: CD4
Gene namesi
Name:Cd4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:88335. Cd4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 394368Extracellular Reviewed prediction
Add
BLAST
Transmembranei395 – 41723Helical; Reviewed prediction
Add
BLAST
Topological domaini418 – 45740Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. endoplasmic reticulum lumen Source: MGI
  3. endoplasmic reticulum membrane Source: MGI
  4. external side of plasma membrane Source: MGI
  5. integral component of membrane Source: UniProtKB-KW
  6. membrane raft Source: MGI
  7. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 Publication
Add
BLAST
Chaini27 – 457431T-cell surface glycoprotein CD4
PRO_0000014627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 1121 Publication
Disulfide bondi159 ↔ 1881 Publication
Glycosylationi187 – 1871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi298 – 2981N-linked (GlcNAc...) Reviewed prediction
Glycosylationi323 – 3231N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi328 ↔ 3701 Publication
Glycosylationi392 – 3921N-linked (GlcNAc...) Reviewed prediction
Lipidationi418 – 4181S-palmitoyl cysteine By similarity
Lipidationi421 – 4211S-palmitoyl cysteine By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP06332.
PRIDEiP06332.

PTM databases

PhosphoSiteiP06332.

Expressioni

Gene expression databases

ArrayExpressiP06332.
BgeeiP06332.
CleanExiMM_CD4.
GenevestigatoriP06332.

Interactioni

Subunit structurei

Associates with p56-LCK. Interacts with SPG21 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
LckP062403EBI-1404,EBI-1401

Protein-protein interaction databases

BioGridi198599. 1 interaction.
IntActiP06332. 3 interactions.
MINTiMINT-5024918.

Structurei

3D structure databases

ProteinModelPortaliP06332.
SMRiP06332. Positions 27-387, 420-453.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 128102Ig-like V-type
Add
BLAST
Domaini129 – 20779Ig-like C2-type 1
Add
BLAST
Domaini208 – 317110Ig-like C2-type 2
Add
BLAST
Domaini318 – 37457Ig-like C2-type 3
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47205.
HOGENOMiHOG000008696.
HOVERGENiHBG005281.
InParanoidiP06332.
KOiK06454.
OMAiPEAGMWQ.
OrthoDBiEOG7HXCQS.
PhylomeDBiP06332.
TreeFamiTF335974.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR000973. Ag_CD4.
IPR015274. CD4-extracel.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
IPR003596. Ig_V-set_subgr.
IPR013151. Immunoglobulin.
IPR021963. Tcell_CD4_Cterm.
[Graphical view]
PfamiPF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF00047. ig. 1 hit.
PF12104. Tcell_CD4_Cterm. 1 hit.
[Graphical view]
PRINTSiPR00692. CD4TCANTIGEN.
SMARTiSM00409. IG. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P06332-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MCRAISLRRL LLLLLQLSQL LAVTQGKTLV LGKEGESAEL PCESSQKKIT    50
VFTWKFSDQR KILGQHGKGV LIRGGSPSQF DRFDSKKGAW EKGSFPLIIN 100
KLKMEDSQTY ICELENRKEE VELWVFKVTF SPGTSLLQGQ SLTLTLDSNS 150
KVSNPLTECK HKKGKVVSGS KVLSMSNLRV QDSDFWNCTV TLDQKKNWFG 200
MTLSVLGFQS TAITAYKSEG ESAEFSFPLN FAEENGWGEL MWKAEKDSFF 250
QPWISFSIKN KEVSVQKSTK DLKLQLKETL PLTLKIPQVS LQFAGSGNLT 300
LTLDKGTLHQ EVNLVVMKVA QLNNTLTCEV MGPTSPKMRL TLKQENQEAR 350
VSEEQKVVQV VAPETGLWQC LLSEGDKVKM DSRIQVLSRG VNQTVFLACV 400
LGGSFGFLGF LGLCILCCVR CRHQQRQAAR MSQIKRLLSE KKTCQCPHRM 450
QKSHNLI 457
Length:457
Mass (Da):51,297
Last modified:January 1, 1988 - v1
Checksum:i1B1DA7527CB00F33
GO
Isoform 2 (identifier: P06332-2) [UniParc]FASTAAdd to Basket

Also known as: Brain-specific

The sequence of this isoform differs from the canonical sequence as follows:
     1-240: Missing.

Show »
Length:217
Mass (Da):24,473
Checksum:i53589C44281646C5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 240240Missing in isoform 2.
VSP_002489Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36850 mRNA. Translation: AAA39401.1.
M13816 mRNA. Translation: AAA37267.1.
X04836 mRNA. Translation: CAA28539.1.
M36851 Genomic DNA. Translation: AAA39402.1.
M17080, M17078, M17079 Genomic DNA. Translation: AAA37403.1.
AC002397 Genomic DNA. Translation: AAC36010.1.
BC039137 mRNA. Translation: AAH39137.1.
CCDSiCCDS20535.1. [P06332-1]
PIRiA02110. RWMST4.
RefSeqiNP_038516.1. NM_013488.2. [P06332-1]
UniGeneiMm.2209.

Genome annotation databases

EnsembliENSMUST00000024044; ENSMUSP00000024044; ENSMUSG00000023274. [P06332-1]
GeneIDi12504.
KEGGimmu:12504.
UCSCiuc009dsi.1. mouse. [P06332-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36850 mRNA. Translation: AAA39401.1 .
M13816 mRNA. Translation: AAA37267.1 .
X04836 mRNA. Translation: CAA28539.1 .
M36851 Genomic DNA. Translation: AAA39402.1 .
M17080 , M17078 , M17079 Genomic DNA. Translation: AAA37403.1 .
AC002397 Genomic DNA. Translation: AAC36010.1 .
BC039137 mRNA. Translation: AAH39137.1 .
CCDSi CCDS20535.1. [P06332-1 ]
PIRi A02110. RWMST4.
RefSeqi NP_038516.1. NM_013488.2. [P06332-1 ]
UniGenei Mm.2209.

3D structure databases

ProteinModelPortali P06332.
SMRi P06332. Positions 27-387, 420-453.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198599. 1 interaction.
IntActi P06332. 3 interactions.
MINTi MINT-5024918.

PTM databases

PhosphoSitei P06332.

Proteomic databases

MaxQBi P06332.
PRIDEi P06332.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024044 ; ENSMUSP00000024044 ; ENSMUSG00000023274 . [P06332-1 ]
GeneIDi 12504.
KEGGi mmu:12504.
UCSCi uc009dsi.1. mouse. [P06332-1 ]

Organism-specific databases

CTDi 920.
MGIi MGI:88335. Cd4.

Phylogenomic databases

eggNOGi NOG47205.
HOGENOMi HOG000008696.
HOVERGENi HBG005281.
InParanoidi P06332.
KOi K06454.
OMAi PEAGMWQ.
OrthoDBi EOG7HXCQS.
PhylomeDBi P06332.
TreeFami TF335974.

Enzyme and pathway databases

Reactomei REACT_215414. Translocation of ZAP-70 to Immunological synapse.
REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
REACT_225145. Downstream TCR signaling.
REACT_225768. Generation of second messenger molecules.

Miscellaneous databases

NextBioi 281462.
PROi P06332.
SOURCEi Search...

Gene expression databases

ArrayExpressi P06332.
Bgeei P06332.
CleanExi MM_CD4.
Genevestigatori P06332.

Family and domain databases

Gene3Di 2.60.40.10. 4 hits.
InterProi IPR000973. Ag_CD4.
IPR015274. CD4-extracel.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
IPR003596. Ig_V-set_subgr.
IPR013151. Immunoglobulin.
IPR021963. Tcell_CD4_Cterm.
[Graphical view ]
Pfami PF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF00047. ig. 1 hit.
PF12104. Tcell_CD4_Cterm. 1 hit.
[Graphical view ]
PRINTSi PR00692. CD4TCANTIGEN.
SMARTi SM00409. IG. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequence of L3T4 complementary DNA clones: expression in T cells and brain."
    Tourvieille B., Gorman S.D., Field E.H., Hunkapiller T., Parnes J.R.
    Science 234:610-614(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Unusual intron in the immunoglobulin domain of the newly isolated murine CD4 (L3T4) gene."
    Littman D.R., Gettner S.N.
    Nature 325:453-455(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "L3T4 and the immunoglobulin gene superfamily: new relationships between the immune system and the nervous system."
    Parnes J.R., Hunkapiller T.
    Immunol. Rev. 100:109-127(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Brain.
  4. "Structure of the mouse gene encoding CD4 and an unusual transcript in brain."
    Gorman S.D., Tourvieille B., Parnes J.R.
    Proc. Natl. Acad. Sci. U.S.A. 84:7644-7648(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
    Tissue: Brain.
  5. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
    Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
    Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  7. "The L3T4 antigen in mouse and the sheep equivalent are immunoglobulin-like."
    Classon B.J., Tsagaratos J., Kirszbaum L., Maddox J., McKay C.R., Brandon M., McKenzie I.F.C., Walker I.D.
    Immunogenetics 23:129-132(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-43.
  8. "Partial primary structure of the T4 antigens of mouse and sheep: assignment of intrachain disulfide bonds."
    Classon B.J., Tsagaratos J., McKenzie I.F.C., Walker I.D.
    Proc. Natl. Acad. Sci. U.S.A. 83:4499-4503(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.

Entry informationi

Entry nameiCD4_MOUSE
AccessioniPrimary (citable) accession number: P06332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 3, 2014
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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