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P06332

- CD4_MOUSE

UniProt

P06332 - CD4_MOUSE

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Protein

T-cell surface glycoprotein CD4

Gene

Cd4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

GO - Molecular functioni

  1. coreceptor activity Source: InterPro
  2. zinc ion binding Source: Ensembl

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. cell surface receptor signaling pathway Source: MGI
  3. cytokine production Source: MGI
  4. defense response to Gram-negative bacterium Source: MGI
  5. immune response Source: InterPro
  6. induction by virus of host cell-cell fusion Source: Ensembl
  7. maintenance of protein location in cell Source: MGI
  8. positive regulation of calcium-mediated signaling Source: MGI
  9. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  10. positive regulation of protein kinase activity Source: Ensembl
  11. positive regulation of T cell activation Source: MGI
  12. protein palmitoleylation Source: MGI
  13. T cell differentiation Source: UniProtKB
  14. T cell selection Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiREACT_215414. Translocation of ZAP-70 to Immunological synapse.
REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
REACT_225145. Downstream TCR signaling.
REACT_225768. Generation of second messenger molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD4
Alternative name(s):
T-cell differentiation antigen L3T4
T-cell surface antigen T4/Leu-3
CD_antigen: CD4
Gene namesi
Name:Cd4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:88335. Cd4.

Subcellular locationi

GO - Cellular componenti

  1. cell surface Source: MGI
  2. endoplasmic reticulum lumen Source: MGI
  3. endoplasmic reticulum membrane Source: MGI
  4. external side of plasma membrane Source: MGI
  5. integral component of membrane Source: UniProtKB-KW
  6. membrane raft Source: MGI
  7. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 457431T-cell surface glycoprotein CD4PRO_0000014627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 1121 PublicationPROSITE-ProRule annotation
Disulfide bondi159 ↔ 1881 PublicationPROSITE-ProRule annotation
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi328 ↔ 3701 PublicationPROSITE-ProRule annotation
Glycosylationi392 – 3921N-linked (GlcNAc...)Sequence Analysis
Lipidationi418 – 4181S-palmitoyl cysteineBy similarity
Lipidationi421 – 4211S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP06332.
PRIDEiP06332.

PTM databases

PhosphoSiteiP06332.

Expressioni

Gene expression databases

BgeeiP06332.
CleanExiMM_CD4.
ExpressionAtlasiP06332. baseline and differential.
GenevestigatoriP06332.

Interactioni

Subunit structurei

Associates with p56-LCK. Interacts with SPG21 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
LckP062403EBI-1404,EBI-1401

Protein-protein interaction databases

BioGridi198599. 1 interaction.
IntActiP06332. 3 interactions.
MINTiMINT-5024918.

Structurei

3D structure databases

ProteinModelPortaliP06332.
SMRiP06332. Positions 27-387, 420-453.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 394368ExtracellularSequence AnalysisAdd
BLAST
Topological domaini418 – 45740CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei395 – 41723HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 128102Ig-like V-typeAdd
BLAST
Domaini129 – 20779Ig-like C2-type 1Add
BLAST
Domaini208 – 317110Ig-like C2-type 2Add
BLAST
Domaini318 – 37457Ig-like C2-type 3Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47205.
HOGENOMiHOG000008696.
HOVERGENiHBG005281.
InParanoidiP06332.
KOiK06454.
OMAiPEAGMWQ.
OrthoDBiEOG7HXCQS.
PhylomeDBiP06332.
TreeFamiTF335974.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR000973. CD4.
IPR015274. CD4-extracel.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
IPR003596. Ig_V-set_subgr.
IPR013151. Immunoglobulin.
IPR021963. Tcell_CD4_Cterm.
[Graphical view]
PfamiPF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF00047. ig. 1 hit.
PF12104. Tcell_CD4_Cterm. 1 hit.
[Graphical view]
PRINTSiPR00692. CD4TCANTIGEN.
SMARTiSM00409. IG. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P06332-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCRAISLRRL LLLLLQLSQL LAVTQGKTLV LGKEGESAEL PCESSQKKIT
60 70 80 90 100
VFTWKFSDQR KILGQHGKGV LIRGGSPSQF DRFDSKKGAW EKGSFPLIIN
110 120 130 140 150
KLKMEDSQTY ICELENRKEE VELWVFKVTF SPGTSLLQGQ SLTLTLDSNS
160 170 180 190 200
KVSNPLTECK HKKGKVVSGS KVLSMSNLRV QDSDFWNCTV TLDQKKNWFG
210 220 230 240 250
MTLSVLGFQS TAITAYKSEG ESAEFSFPLN FAEENGWGEL MWKAEKDSFF
260 270 280 290 300
QPWISFSIKN KEVSVQKSTK DLKLQLKETL PLTLKIPQVS LQFAGSGNLT
310 320 330 340 350
LTLDKGTLHQ EVNLVVMKVA QLNNTLTCEV MGPTSPKMRL TLKQENQEAR
360 370 380 390 400
VSEEQKVVQV VAPETGLWQC LLSEGDKVKM DSRIQVLSRG VNQTVFLACV
410 420 430 440 450
LGGSFGFLGF LGLCILCCVR CRHQQRQAAR MSQIKRLLSE KKTCQCPHRM

QKSHNLI
Length:457
Mass (Da):51,297
Last modified:January 1, 1988 - v1
Checksum:i1B1DA7527CB00F33
GO
Isoform 2 (identifier: P06332-2) [UniParc]FASTAAdd to Basket

Also known as: Brain-specific

The sequence of this isoform differs from the canonical sequence as follows:
     1-240: Missing.

Show »
Length:217
Mass (Da):24,473
Checksum:i53589C44281646C5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 240240Missing in isoform 2. CuratedVSP_002489Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36850 mRNA. Translation: AAA39401.1.
M13816 mRNA. Translation: AAA37267.1.
X04836 mRNA. Translation: CAA28539.1.
M36851 Genomic DNA. Translation: AAA39402.1.
M17080, M17078, M17079 Genomic DNA. Translation: AAA37403.1.
AC002397 Genomic DNA. Translation: AAC36010.1.
BC039137 mRNA. Translation: AAH39137.1.
CCDSiCCDS20535.1. [P06332-1]
PIRiA02110. RWMST4.
RefSeqiNP_038516.1. NM_013488.2. [P06332-1]
UniGeneiMm.2209.

Genome annotation databases

EnsembliENSMUST00000024044; ENSMUSP00000024044; ENSMUSG00000023274. [P06332-1]
GeneIDi12504.
KEGGimmu:12504.
UCSCiuc009dsi.1. mouse. [P06332-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36850 mRNA. Translation: AAA39401.1 .
M13816 mRNA. Translation: AAA37267.1 .
X04836 mRNA. Translation: CAA28539.1 .
M36851 Genomic DNA. Translation: AAA39402.1 .
M17080 , M17078 , M17079 Genomic DNA. Translation: AAA37403.1 .
AC002397 Genomic DNA. Translation: AAC36010.1 .
BC039137 mRNA. Translation: AAH39137.1 .
CCDSi CCDS20535.1. [P06332-1 ]
PIRi A02110. RWMST4.
RefSeqi NP_038516.1. NM_013488.2. [P06332-1 ]
UniGenei Mm.2209.

3D structure databases

ProteinModelPortali P06332.
SMRi P06332. Positions 27-387, 420-453.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198599. 1 interaction.
IntActi P06332. 3 interactions.
MINTi MINT-5024918.

PTM databases

PhosphoSitei P06332.

Proteomic databases

MaxQBi P06332.
PRIDEi P06332.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024044 ; ENSMUSP00000024044 ; ENSMUSG00000023274 . [P06332-1 ]
GeneIDi 12504.
KEGGi mmu:12504.
UCSCi uc009dsi.1. mouse. [P06332-1 ]

Organism-specific databases

CTDi 920.
MGIi MGI:88335. Cd4.

Phylogenomic databases

eggNOGi NOG47205.
HOGENOMi HOG000008696.
HOVERGENi HBG005281.
InParanoidi P06332.
KOi K06454.
OMAi PEAGMWQ.
OrthoDBi EOG7HXCQS.
PhylomeDBi P06332.
TreeFami TF335974.

Enzyme and pathway databases

Reactomei REACT_215414. Translocation of ZAP-70 to Immunological synapse.
REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
REACT_225145. Downstream TCR signaling.
REACT_225768. Generation of second messenger molecules.

Miscellaneous databases

NextBioi 281462.
PROi P06332.
SOURCEi Search...

Gene expression databases

Bgeei P06332.
CleanExi MM_CD4.
ExpressionAtlasi P06332. baseline and differential.
Genevestigatori P06332.

Family and domain databases

Gene3Di 2.60.40.10. 4 hits.
InterProi IPR000973. CD4.
IPR015274. CD4-extracel.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
IPR003596. Ig_V-set_subgr.
IPR013151. Immunoglobulin.
IPR021963. Tcell_CD4_Cterm.
[Graphical view ]
Pfami PF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF00047. ig. 1 hit.
PF12104. Tcell_CD4_Cterm. 1 hit.
[Graphical view ]
PRINTSi PR00692. CD4TCANTIGEN.
SMARTi SM00409. IG. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequence of L3T4 complementary DNA clones: expression in T cells and brain."
    Tourvieille B., Gorman S.D., Field E.H., Hunkapiller T., Parnes J.R.
    Science 234:610-614(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Unusual intron in the immunoglobulin domain of the newly isolated murine CD4 (L3T4) gene."
    Littman D.R., Gettner S.N.
    Nature 325:453-455(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "L3T4 and the immunoglobulin gene superfamily: new relationships between the immune system and the nervous system."
    Parnes J.R., Hunkapiller T.
    Immunol. Rev. 100:109-127(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Brain.
  4. "Structure of the mouse gene encoding CD4 and an unusual transcript in brain."
    Gorman S.D., Tourvieille B., Parnes J.R.
    Proc. Natl. Acad. Sci. U.S.A. 84:7644-7648(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
    Tissue: Brain.
  5. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
    Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
    Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  7. "The L3T4 antigen in mouse and the sheep equivalent are immunoglobulin-like."
    Classon B.J., Tsagaratos J., Kirszbaum L., Maddox J., McKay C.R., Brandon M., McKenzie I.F.C., Walker I.D.
    Immunogenetics 23:129-132(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-43.
  8. "Partial primary structure of the T4 antigens of mouse and sheep: assignment of intrachain disulfide bonds."
    Classon B.J., Tsagaratos J., McKenzie I.F.C., Walker I.D.
    Proc. Natl. Acad. Sci. U.S.A. 83:4499-4503(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.

Entry informationi

Entry nameiCD4_MOUSE
AccessioniPrimary (citable) accession number: P06332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 29, 2014
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3