P06332 (CD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-cell surface glycoprotein CD4

Alternative name(s):
T-cell differentiation antigen L3T4
T-cell surface antigen T4/Leu-3
CD_antigen=CD4

Gene names

Name:

Cd4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.
Subunit structure	Associates with p56-LCK. Interacts with SPG21 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	T cell selection Inferred from sequence or structural similarity PubMed 9551897. Source: UniProtKB cell adhesion Inferred from electronic annotation. Source: InterPro cell surface receptor signaling pathway Inferred from direct assay PubMed 9208839. Source: MGI cytokine production Inferred from mutant phenotype PubMed 7930593. Source: MGI defense response to Gram-negative bacterium Inferred from mutant phenotype PubMed 14688352. Source: MGI immune response Inferred from electronic annotation. Source: InterPro induction by virus of host cell-cell fusion Inferred from electronic annotation. Source: Compara maintenance of protein location in cell Inferred from sequence orthology PubMed 15128768. Source: MGI positive regulation of T cell activation Inferred from direct assay PubMed 12421911. Source: MGI positive regulation of calcium-mediated signaling Inferred from direct assay PubMed 9208839. Source: MGI positive regulation of peptidyl-tyrosine phosphorylation Inferred from direct assay PubMed 9208839. Source: MGI positive regulation of protein kinase activity Inferred from electronic annotation. Source: Compara protein palmitoleylation Inferred from sequence orthology PubMed 15128768. Source: MGI
Cellular_component	endoplasmic reticulum lumen Inferred from direct assay PubMed 16223484. Source: MGI endoplasmic reticulum membrane Inferred from direct assay PubMed 16223484. Source: MGI external side of plasma membrane Inferred from direct assay PubMed 10072077PubMed 10704459PubMed 10943842PubMed 12421911PubMed 14609575PubMed 15322158PubMed 15368291PubMed 15728238PubMed 15795238PubMed 16287714PubMed 16455951PubMed 16973387PubMed 17082577PubMed 17357106PubMed 18641307PubMed 18776904PubMed 18836449PubMed 19008373PubMed 19349303PubMed 19723499PubMed 19838199PubMed 19934022PubMed 20399120PubMed 20706986PubMed 20709950PubMed 21460847PubMed 21508411PubMed 22072979PubMed 7477352PubMed 7477353PubMed 7589135PubMed 7630421PubMed 7688139PubMed 8524870PubMed 8755570PubMed 8769481PubMed 8788039PubMed 9133426PubMed 9208839. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from direct assay PubMed 15128768. Source: MGI
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P06332-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P06332-2)

Also known as: Brain-specific;

The sequence of this isoform differs from the canonical sequence as follows:
     1-240: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.7
	Chain	27 – 457	431	T-cell surface glycoprotein CD4		PRO_0000014627
Regions
	Topological domain	27 – 394	368	Extracellular Potential
	Transmembrane	395 – 417	23	Helical; Potential
	Topological domain	418 – 457	40	Cytoplasmic Potential
	Domain	27 – 128	102	Ig-like V-type
	Domain	129 – 207	79	Ig-like C2-type 1
	Domain	208 – 317	110	Ig-like C2-type 2
	Domain	318 – 374	57	Ig-like C2-type 3
Amino acid modifications
	Lipidation	418	1	S-palmitoyl cysteine By similarity
	Lipidation	421	1	S-palmitoyl cysteine By similarity
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Glycosylation	392	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 112		Ref.8
	Disulfide bond	159 ↔ 188		Ref.8
	Disulfide bond	328 ↔ 370		Ref.8
Natural variations
	Alternative sequence	1 – 240	240	Missing in isoform 2.		VSP_002489

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 1B1DA7527CB00F33	FASTA	457	51,297
	10 20 30 40 50 60 MCRAISLRRL LLLLLQLSQL LAVTQGKTLV LGKEGESAEL PCESSQKKIT VFTWKFSDQR 70 80 90 100 110 120 KILGQHGKGV LIRGGSPSQF DRFDSKKGAW EKGSFPLIIN KLKMEDSQTY ICELENRKEE 130 140 150 160 170 180 VELWVFKVTF SPGTSLLQGQ SLTLTLDSNS KVSNPLTECK HKKGKVVSGS KVLSMSNLRV 190 200 210 220 230 240 QDSDFWNCTV TLDQKKNWFG MTLSVLGFQS TAITAYKSEG ESAEFSFPLN FAEENGWGEL 250 260 270 280 290 300 MWKAEKDSFF QPWISFSIKN KEVSVQKSTK DLKLQLKETL PLTLKIPQVS LQFAGSGNLT 310 320 330 340 350 360 LTLDKGTLHQ EVNLVVMKVA QLNNTLTCEV MGPTSPKMRL TLKQENQEAR VSEEQKVVQV 370 380 390 400 410 420 VAPETGLWQC LLSEGDKVKM DSRIQVLSRG VNQTVFLACV LGGSFGFLGF LGLCILCCVR 430 440 450 CRHQQRQAAR MSQIKRLLSE KKTCQCPHRM QKSHNLI
	Isoform 2 (Brain-specific) [UniParc]. Checksum: 53589C44281646C5	FASTA	217	24,473
	10 20 30 40 50 60 MWKAEKDSFF QPWISFSIKN KEVSVQKSTK DLKLQLKETL PLTLKIPQVS LQFAGSGNLT 70 80 90 100 110 120 LTLDKGTLHQ EVNLVVMKVA QLNNTLTCEV MGPTSPKMRL TLKQENQEAR VSEEQKVVQV 130 140 150 160 170 180 VAPETGLWQC LLSEGDKVKM DSRIQVLSRG VNQTVFLACV LGGSFGFLGF LGLCILCCVR 190 200 210 CRHQQRQAAR MSQIKRLLSE KKTCQCPHRM QKSHNLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and sequence of L3T4 complementary DNA clones: expression in T cells and brain." Tourvieille B., Gorman S.D., Field E.H., Hunkapiller T., Parnes J.R. Science 234:610-614(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Unusual intron in the immunoglobulin domain of the newly isolated murine CD4 (L3T4) gene." Littman D.R., Gettner S.N. Nature 325:453-455(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"L3T4 and the immunoglobulin gene superfamily: new relationships between the immune system and the nervous system." Parnes J.R., Hunkapiller T. Immunol. Rev. 100:109-127(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Brain.
[4]	"Structure of the mouse gene encoding CD4 and an unusual transcript in brain." Gorman S.D., Tourvieille B., Parnes J.R. Proc. Natl. Acad. Sci. U.S.A. 84:7644-7648(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2). Tissue: Brain.
[5]	"Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6." Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A. Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Mammary gland.
[7]	"The L3T4 antigen in mouse and the sheep equivalent are immunoglobulin-like." Classon B.J., Tsagaratos J., Kirszbaum L., Maddox J., McKay C.R., Brandon M., McKenzie I.F.C., Walker I.D. Immunogenetics 23:129-132(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-43.
[8]	"Partial primary structure of the T4 antigens of mouse and sheep: assignment of intrachain disulfide bonds." Classon B.J., Tsagaratos J., McKenzie I.F.C., Walker I.D. Proc. Natl. Acad. Sci. U.S.A. 83:4499-4503(1986) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36850 mRNA. Translation: AAA39401.1. M13816 mRNA. Translation: AAA37267.1. X04836 mRNA. Translation: CAA28539.1. M36851 Genomic DNA. Translation: AAA39402.1. M17080, M17078, M17079 Genomic DNA. Translation: AAA37403.1. AC002397 Genomic DNA. Translation: AAC36010.1. BC039137 mRNA. Translation: AAH39137.1.
IPI	IPI00122806. IPI00229336.
PIR	RWMST4. A02110.
RefSeq	NP_038516.1. NM_013488.2.
UniGene	Mm.2209.
3D structure databases
ProteinModelPortal	P06332.
SMR	P06332. Positions 27-387, 420-453.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06332. 2 interactions.
PTM databases
PhosphoSite	P06332.
Proteomic databases
PRIDE	P06332.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000024044; ENSMUSP00000024044; ENSMUSG00000023274.
GeneID	12504.
KEGG	mmu:12504.
UCSC	uc012esr.1. mouse.
Organism-specific databases
CTD	920.
MGI	MGI:88335. Cd4.
Phylogenomic databases
eggNOG	NOG47205.
HOGENOM	HOG000008696.
HOVERGEN	HBG005281.
InParanoid	P06332.
KO	K06454.
OMA	CEVWGPT.
OrthoDB	EOG4NVZM4.
Gene expression databases
ArrayExpress	P06332.
Bgee	P06332.
CleanEx	MM_CD4.
Genevestigator	P06332.
GermOnline	ENSMUSG00000023274. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 4 hits.
InterPro	IPR000973. Ag_CD4. IPR015274. CD4-extracel. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR008424. Ig_C2-set. IPR003599. Ig_sub. IPR003596. Ig_V-set_subgr. IPR013151. Immunoglobulin. IPR021963. Tcell_CD4_Cterm. [Graphical view]
Pfam	PF05790. C2-set. 2 hits. PF09191. CD4-extracel. 1 hit. PF00047. ig. 1 hit. PF12104. Tcell_CD4_Cterm. 1 hit. [Graphical view]
PRINTS	PR00692. CD4TCANTIGEN.
SMART	SM00409. IG. 2 hits. SM00406. IGv. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281462.
SOURCE	Search...

Entry information

Entry name

CD4_MOUSE

Accession

Primary (citable) accession number: P06332

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	May 1, 2013
This is version 134 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program