Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P06332

- CD4_MOUSE

UniProt

P06332 - CD4_MOUSE

Protein

T-cell surface glycoprotein CD4

Gene

Cd4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. zinc ion binding Source: Ensembl

    GO - Biological processi

    1. cell adhesion Source: InterPro
    2. cell surface receptor signaling pathway Source: MGI
    3. cytokine production Source: MGI
    4. defense response to Gram-negative bacterium Source: MGI
    5. immune response Source: InterPro
    6. induction by virus of host cell-cell fusion Source: Ensembl
    7. maintenance of protein location in cell Source: MGI
    8. positive regulation of calcium-mediated signaling Source: MGI
    9. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
    10. positive regulation of protein kinase activity Source: Ensembl
    11. positive regulation of T cell activation Source: MGI
    12. protein palmitoleylation Source: MGI
    13. T cell differentiation Source: UniProtKB
    14. T cell selection Source: UniProtKB

    Keywords - Biological processi

    Adaptive immunity, Immunity

    Enzyme and pathway databases

    ReactomeiREACT_215414. Translocation of ZAP-70 to Immunological synapse.
    REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
    REACT_225145. Downstream TCR signaling.
    REACT_225768. Generation of second messenger molecules.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-cell surface glycoprotein CD4
    Alternative name(s):
    T-cell differentiation antigen L3T4
    T-cell surface antigen T4/Leu-3
    CD_antigen: CD4
    Gene namesi
    Name:Cd4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:88335. Cd4.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: MGI
    2. endoplasmic reticulum lumen Source: MGI
    3. endoplasmic reticulum membrane Source: MGI
    4. external side of plasma membrane Source: MGI
    5. integral component of membrane Source: UniProtKB-KW
    6. membrane raft Source: MGI
    7. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 PublicationAdd
    BLAST
    Chaini27 – 457431T-cell surface glycoprotein CD4PRO_0000014627Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi42 ↔ 1121 PublicationPROSITE-ProRule annotation
    Disulfide bondi159 ↔ 1881 PublicationPROSITE-ProRule annotation
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi328 ↔ 3701 PublicationPROSITE-ProRule annotation
    Glycosylationi392 – 3921N-linked (GlcNAc...)Sequence Analysis
    Lipidationi418 – 4181S-palmitoyl cysteineBy similarity
    Lipidationi421 – 4211S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    MaxQBiP06332.
    PRIDEiP06332.

    PTM databases

    PhosphoSiteiP06332.

    Expressioni

    Gene expression databases

    ArrayExpressiP06332.
    BgeeiP06332.
    CleanExiMM_CD4.
    GenevestigatoriP06332.

    Interactioni

    Subunit structurei

    Associates with p56-LCK. Interacts with SPG21 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LckP062403EBI-1404,EBI-1401

    Protein-protein interaction databases

    BioGridi198599. 1 interaction.
    IntActiP06332. 3 interactions.
    MINTiMINT-5024918.

    Structurei

    3D structure databases

    ProteinModelPortaliP06332.
    SMRiP06332. Positions 27-387, 420-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 394368ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini418 – 45740CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei395 – 41723HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 128102Ig-like V-typeAdd
    BLAST
    Domaini129 – 20779Ig-like C2-type 1Add
    BLAST
    Domaini208 – 317110Ig-like C2-type 2Add
    BLAST
    Domaini318 – 37457Ig-like C2-type 3Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG47205.
    HOGENOMiHOG000008696.
    HOVERGENiHBG005281.
    InParanoidiP06332.
    KOiK06454.
    OMAiPEAGMWQ.
    OrthoDBiEOG7HXCQS.
    PhylomeDBiP06332.
    TreeFamiTF335974.

    Family and domain databases

    Gene3Di2.60.40.10. 4 hits.
    InterProiIPR000973. Ag_CD4.
    IPR015274. CD4-extracel.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR008424. Ig_C2-set.
    IPR003599. Ig_sub.
    IPR003596. Ig_V-set_subgr.
    IPR013151. Immunoglobulin.
    IPR021963. Tcell_CD4_Cterm.
    [Graphical view]
    PfamiPF05790. C2-set. 2 hits.
    PF09191. CD4-extracel. 1 hit.
    PF00047. ig. 1 hit.
    PF12104. Tcell_CD4_Cterm. 1 hit.
    [Graphical view]
    PRINTSiPR00692. CD4TCANTIGEN.
    SMARTiSM00409. IG. 2 hits.
    SM00406. IGv. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P06332-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCRAISLRRL LLLLLQLSQL LAVTQGKTLV LGKEGESAEL PCESSQKKIT    50
    VFTWKFSDQR KILGQHGKGV LIRGGSPSQF DRFDSKKGAW EKGSFPLIIN 100
    KLKMEDSQTY ICELENRKEE VELWVFKVTF SPGTSLLQGQ SLTLTLDSNS 150
    KVSNPLTECK HKKGKVVSGS KVLSMSNLRV QDSDFWNCTV TLDQKKNWFG 200
    MTLSVLGFQS TAITAYKSEG ESAEFSFPLN FAEENGWGEL MWKAEKDSFF 250
    QPWISFSIKN KEVSVQKSTK DLKLQLKETL PLTLKIPQVS LQFAGSGNLT 300
    LTLDKGTLHQ EVNLVVMKVA QLNNTLTCEV MGPTSPKMRL TLKQENQEAR 350
    VSEEQKVVQV VAPETGLWQC LLSEGDKVKM DSRIQVLSRG VNQTVFLACV 400
    LGGSFGFLGF LGLCILCCVR CRHQQRQAAR MSQIKRLLSE KKTCQCPHRM 450
    QKSHNLI 457
    Length:457
    Mass (Da):51,297
    Last modified:January 1, 1988 - v1
    Checksum:i1B1DA7527CB00F33
    GO
    Isoform 2 (identifier: P06332-2) [UniParc]FASTAAdd to Basket

    Also known as: Brain-specific

    The sequence of this isoform differs from the canonical sequence as follows:
         1-240: Missing.

    Show »
    Length:217
    Mass (Da):24,473
    Checksum:i53589C44281646C5
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 240240Missing in isoform 2. CuratedVSP_002489Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36850 mRNA. Translation: AAA39401.1.
    M13816 mRNA. Translation: AAA37267.1.
    X04836 mRNA. Translation: CAA28539.1.
    M36851 Genomic DNA. Translation: AAA39402.1.
    M17080, M17078, M17079 Genomic DNA. Translation: AAA37403.1.
    AC002397 Genomic DNA. Translation: AAC36010.1.
    BC039137 mRNA. Translation: AAH39137.1.
    CCDSiCCDS20535.1. [P06332-1]
    PIRiA02110. RWMST4.
    RefSeqiNP_038516.1. NM_013488.2. [P06332-1]
    UniGeneiMm.2209.

    Genome annotation databases

    EnsembliENSMUST00000024044; ENSMUSP00000024044; ENSMUSG00000023274. [P06332-1]
    GeneIDi12504.
    KEGGimmu:12504.
    UCSCiuc009dsi.1. mouse. [P06332-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36850 mRNA. Translation: AAA39401.1 .
    M13816 mRNA. Translation: AAA37267.1 .
    X04836 mRNA. Translation: CAA28539.1 .
    M36851 Genomic DNA. Translation: AAA39402.1 .
    M17080 , M17078 , M17079 Genomic DNA. Translation: AAA37403.1 .
    AC002397 Genomic DNA. Translation: AAC36010.1 .
    BC039137 mRNA. Translation: AAH39137.1 .
    CCDSi CCDS20535.1. [P06332-1 ]
    PIRi A02110. RWMST4.
    RefSeqi NP_038516.1. NM_013488.2. [P06332-1 ]
    UniGenei Mm.2209.

    3D structure databases

    ProteinModelPortali P06332.
    SMRi P06332. Positions 27-387, 420-453.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198599. 1 interaction.
    IntActi P06332. 3 interactions.
    MINTi MINT-5024918.

    PTM databases

    PhosphoSitei P06332.

    Proteomic databases

    MaxQBi P06332.
    PRIDEi P06332.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024044 ; ENSMUSP00000024044 ; ENSMUSG00000023274 . [P06332-1 ]
    GeneIDi 12504.
    KEGGi mmu:12504.
    UCSCi uc009dsi.1. mouse. [P06332-1 ]

    Organism-specific databases

    CTDi 920.
    MGIi MGI:88335. Cd4.

    Phylogenomic databases

    eggNOGi NOG47205.
    HOGENOMi HOG000008696.
    HOVERGENi HBG005281.
    InParanoidi P06332.
    KOi K06454.
    OMAi PEAGMWQ.
    OrthoDBi EOG7HXCQS.
    PhylomeDBi P06332.
    TreeFami TF335974.

    Enzyme and pathway databases

    Reactomei REACT_215414. Translocation of ZAP-70 to Immunological synapse.
    REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
    REACT_225145. Downstream TCR signaling.
    REACT_225768. Generation of second messenger molecules.

    Miscellaneous databases

    NextBioi 281462.
    PROi P06332.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P06332.
    Bgeei P06332.
    CleanExi MM_CD4.
    Genevestigatori P06332.

    Family and domain databases

    Gene3Di 2.60.40.10. 4 hits.
    InterProi IPR000973. Ag_CD4.
    IPR015274. CD4-extracel.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR008424. Ig_C2-set.
    IPR003599. Ig_sub.
    IPR003596. Ig_V-set_subgr.
    IPR013151. Immunoglobulin.
    IPR021963. Tcell_CD4_Cterm.
    [Graphical view ]
    Pfami PF05790. C2-set. 2 hits.
    PF09191. CD4-extracel. 1 hit.
    PF00047. ig. 1 hit.
    PF12104. Tcell_CD4_Cterm. 1 hit.
    [Graphical view ]
    PRINTSi PR00692. CD4TCANTIGEN.
    SMARTi SM00409. IG. 2 hits.
    SM00406. IGv. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and sequence of L3T4 complementary DNA clones: expression in T cells and brain."
      Tourvieille B., Gorman S.D., Field E.H., Hunkapiller T., Parnes J.R.
      Science 234:610-614(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Unusual intron in the immunoglobulin domain of the newly isolated murine CD4 (L3T4) gene."
      Littman D.R., Gettner S.N.
      Nature 325:453-455(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "L3T4 and the immunoglobulin gene superfamily: new relationships between the immune system and the nervous system."
      Parnes J.R., Hunkapiller T.
      Immunol. Rev. 100:109-127(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Brain.
    4. "Structure of the mouse gene encoding CD4 and an unusual transcript in brain."
      Gorman S.D., Tourvieille B., Parnes J.R.
      Proc. Natl. Acad. Sci. U.S.A. 84:7644-7648(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
      Tissue: Brain.
    5. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
      Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
      Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    7. "The L3T4 antigen in mouse and the sheep equivalent are immunoglobulin-like."
      Classon B.J., Tsagaratos J., Kirszbaum L., Maddox J., McKay C.R., Brandon M., McKenzie I.F.C., Walker I.D.
      Immunogenetics 23:129-132(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 27-43.
    8. "Partial primary structure of the T4 antigens of mouse and sheep: assignment of intrachain disulfide bonds."
      Classon B.J., Tsagaratos J., McKenzie I.F.C., Walker I.D.
      Proc. Natl. Acad. Sci. U.S.A. 83:4499-4503(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.

    Entry informationi

    Entry nameiCD4_MOUSE
    AccessioniPrimary (citable) accession number: P06332
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 147 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3