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Protein

T-cell surface glycoprotein CD4

Gene

Cd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

GO - Molecular functioni

GO - Biological processi

  • cell surface receptor signaling pathway Source: MGI
  • cytokine production Source: MGI
  • defense response to Gram-negative bacterium Source: MGI
  • immune response Source: InterPro
  • induction by virus of host cell-cell fusion Source: MGI
  • maintenance of protein location in cell Source: MGI
  • positive regulation of calcium-mediated signaling Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of protein kinase activity Source: MGI
  • positive regulation of T cell activation Source: MGI
  • protein palmitoleylation Source: MGI
  • regulation of T cell activation Source: MGI
  • T cell differentiation Source: UniProtKB
  • T cell selection Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiREACT_280649. Downstream TCR signaling.
REACT_309115. PD-1 signaling.
REACT_319579. Alpha-defensins.
REACT_334692. Generation of second messenger molecules.
REACT_345130. Translocation of ZAP-70 to Immunological synapse.
REACT_345471. Phosphorylation of CD3 and TCR zeta chains.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD4
Alternative name(s):
T-cell differentiation antigen L3T4
T-cell surface antigen T4/Leu-3
CD_antigen: CD4
Gene namesi
Name:Cd4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88335. Cd4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 394368ExtracellularSequence AnalysisAdd
BLAST
Transmembranei395 – 41723HelicalSequence AnalysisAdd
BLAST
Topological domaini418 – 45740CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 457431T-cell surface glycoprotein CD4PRO_0000014627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 112PROSITE-ProRule annotation1 Publication
Disulfide bondi159 ↔ 188PROSITE-ProRule annotation1 Publication
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi328 ↔ 370PROSITE-ProRule annotation1 Publication
Glycosylationi392 – 3921N-linked (GlcNAc...)Sequence Analysis
Lipidationi418 – 4181S-palmitoyl cysteineBy similarity
Lipidationi421 – 4211S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP06332.
PRIDEiP06332.

PTM databases

PhosphoSiteiP06332.

Expressioni

Gene expression databases

BgeeiP06332.
CleanExiMM_CD4.
ExpressionAtlasiP06332. baseline and differential.
GenevisibleiP06332. MM.

Interactioni

Subunit structurei

Associates with p56-LCK. Interacts with SPG21 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
LckP062403EBI-1404,EBI-1401

Protein-protein interaction databases

BioGridi198599. 1 interaction.
IntActiP06332. 3 interactions.
MINTiMINT-5024918.
STRINGi10090.ENSMUSP00000024044.

Structurei

3D structure databases

ProteinModelPortaliP06332.
SMRiP06332. Positions 27-387, 420-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 128102Ig-like V-typeAdd
BLAST
Domaini129 – 20779Ig-like C2-type 1Add
BLAST
Domaini208 – 317110Ig-like C2-type 2Add
BLAST
Domaini318 – 37457Ig-like C2-type 3Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47205.
HOGENOMiHOG000008696.
HOVERGENiHBG005281.
InParanoidiP06332.
KOiK06454.
OMAiPEAGMWQ.
OrthoDBiEOG7HXCQS.
PhylomeDBiP06332.
TreeFamiTF335974.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR000973. CD4.
IPR015274. CD4-extracel.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
IPR021963. Tcell_CD4_Cterm.
[Graphical view]
PfamiPF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF00047. ig. 1 hit.
PF12104. Tcell_CD4_Cterm. 1 hit.
[Graphical view]
PRINTSiPR00692. CD4TCANTIGEN.
SMARTiSM00409. IG. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P06332-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCRAISLRRL LLLLLQLSQL LAVTQGKTLV LGKEGESAEL PCESSQKKIT
60 70 80 90 100
VFTWKFSDQR KILGQHGKGV LIRGGSPSQF DRFDSKKGAW EKGSFPLIIN
110 120 130 140 150
KLKMEDSQTY ICELENRKEE VELWVFKVTF SPGTSLLQGQ SLTLTLDSNS
160 170 180 190 200
KVSNPLTECK HKKGKVVSGS KVLSMSNLRV QDSDFWNCTV TLDQKKNWFG
210 220 230 240 250
MTLSVLGFQS TAITAYKSEG ESAEFSFPLN FAEENGWGEL MWKAEKDSFF
260 270 280 290 300
QPWISFSIKN KEVSVQKSTK DLKLQLKETL PLTLKIPQVS LQFAGSGNLT
310 320 330 340 350
LTLDKGTLHQ EVNLVVMKVA QLNNTLTCEV MGPTSPKMRL TLKQENQEAR
360 370 380 390 400
VSEEQKVVQV VAPETGLWQC LLSEGDKVKM DSRIQVLSRG VNQTVFLACV
410 420 430 440 450
LGGSFGFLGF LGLCILCCVR CRHQQRQAAR MSQIKRLLSE KKTCQCPHRM

QKSHNLI
Length:457
Mass (Da):51,297
Last modified:January 1, 1988 - v1
Checksum:i1B1DA7527CB00F33
GO
Isoform 2 (identifier: P06332-2) [UniParc]FASTAAdd to basket

Also known as: Brain-specific

The sequence of this isoform differs from the canonical sequence as follows:
     1-240: Missing.

Show »
Length:217
Mass (Da):24,473
Checksum:i53589C44281646C5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 240240Missing in isoform 2. CuratedVSP_002489Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36850 mRNA. Translation: AAA39401.1.
M13816 mRNA. Translation: AAA37267.1.
X04836 mRNA. Translation: CAA28539.1.
M36851 Genomic DNA. Translation: AAA39402.1.
M17080, M17078, M17079 Genomic DNA. Translation: AAA37403.1.
AC002397 Genomic DNA. Translation: AAC36010.1.
BC039137 mRNA. Translation: AAH39137.1.
CCDSiCCDS20535.1. [P06332-1]
PIRiA02110. RWMST4.
RefSeqiNP_038516.1. NM_013488.2. [P06332-1]
UniGeneiMm.2209.

Genome annotation databases

EnsembliENSMUST00000024044; ENSMUSP00000024044; ENSMUSG00000023274. [P06332-1]
GeneIDi12504.
KEGGimmu:12504.
UCSCiuc009dsi.1. mouse. [P06332-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36850 mRNA. Translation: AAA39401.1.
M13816 mRNA. Translation: AAA37267.1.
X04836 mRNA. Translation: CAA28539.1.
M36851 Genomic DNA. Translation: AAA39402.1.
M17080, M17078, M17079 Genomic DNA. Translation: AAA37403.1.
AC002397 Genomic DNA. Translation: AAC36010.1.
BC039137 mRNA. Translation: AAH39137.1.
CCDSiCCDS20535.1. [P06332-1]
PIRiA02110. RWMST4.
RefSeqiNP_038516.1. NM_013488.2. [P06332-1]
UniGeneiMm.2209.

3D structure databases

ProteinModelPortaliP06332.
SMRiP06332. Positions 27-387, 420-453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198599. 1 interaction.
IntActiP06332. 3 interactions.
MINTiMINT-5024918.
STRINGi10090.ENSMUSP00000024044.

PTM databases

PhosphoSiteiP06332.

Proteomic databases

MaxQBiP06332.
PRIDEiP06332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024044; ENSMUSP00000024044; ENSMUSG00000023274. [P06332-1]
GeneIDi12504.
KEGGimmu:12504.
UCSCiuc009dsi.1. mouse. [P06332-1]

Organism-specific databases

CTDi920.
MGIiMGI:88335. Cd4.

Phylogenomic databases

eggNOGiNOG47205.
HOGENOMiHOG000008696.
HOVERGENiHBG005281.
InParanoidiP06332.
KOiK06454.
OMAiPEAGMWQ.
OrthoDBiEOG7HXCQS.
PhylomeDBiP06332.
TreeFamiTF335974.

Enzyme and pathway databases

ReactomeiREACT_280649. Downstream TCR signaling.
REACT_309115. PD-1 signaling.
REACT_319579. Alpha-defensins.
REACT_334692. Generation of second messenger molecules.
REACT_345130. Translocation of ZAP-70 to Immunological synapse.
REACT_345471. Phosphorylation of CD3 and TCR zeta chains.

Miscellaneous databases

NextBioi281462.
PROiP06332.
SOURCEiSearch...

Gene expression databases

BgeeiP06332.
CleanExiMM_CD4.
ExpressionAtlasiP06332. baseline and differential.
GenevisibleiP06332. MM.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR000973. CD4.
IPR015274. CD4-extracel.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
IPR021963. Tcell_CD4_Cterm.
[Graphical view]
PfamiPF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF00047. ig. 1 hit.
PF12104. Tcell_CD4_Cterm. 1 hit.
[Graphical view]
PRINTSiPR00692. CD4TCANTIGEN.
SMARTiSM00409. IG. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequence of L3T4 complementary DNA clones: expression in T cells and brain."
    Tourvieille B., Gorman S.D., Field E.H., Hunkapiller T., Parnes J.R.
    Science 234:610-614(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Unusual intron in the immunoglobulin domain of the newly isolated murine CD4 (L3T4) gene."
    Littman D.R., Gettner S.N.
    Nature 325:453-455(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "L3T4 and the immunoglobulin gene superfamily: new relationships between the immune system and the nervous system."
    Parnes J.R., Hunkapiller T.
    Immunol. Rev. 100:109-127(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Brain.
  4. "Structure of the mouse gene encoding CD4 and an unusual transcript in brain."
    Gorman S.D., Tourvieille B., Parnes J.R.
    Proc. Natl. Acad. Sci. U.S.A. 84:7644-7648(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
    Tissue: Brain.
  5. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
    Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
    Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  7. "The L3T4 antigen in mouse and the sheep equivalent are immunoglobulin-like."
    Classon B.J., Tsagaratos J., Kirszbaum L., Maddox J., McKay C.R., Brandon M., McKenzie I.F.C., Walker I.D.
    Immunogenetics 23:129-132(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-43.
  8. "Partial primary structure of the T4 antigens of mouse and sheep: assignment of intrachain disulfide bonds."
    Classon B.J., Tsagaratos J., McKenzie I.F.C., Walker I.D.
    Proc. Natl. Acad. Sci. U.S.A. 83:4499-4503(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.

Entry informationi

Entry nameiCD4_MOUSE
AccessioniPrimary (citable) accession number: P06332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 24, 2015
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.