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P06307

- CCKN_HUMAN

UniProt

P06307 - CCKN_HUMAN

Protein

Cholecystokinin

Gene

CCK

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.

    GO - Molecular functioni

    1. hormone activity Source: ProtInc
    2. neuropeptide hormone activity Source: UniProtKB
    3. protein binding Source: IntAct

    GO - Biological processi

    1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    2. axonogenesis Source: UniProtKB
    3. behavioral fear response Source: Ensembl
    4. eating behavior Source: UniProtKB
    5. negative regulation of appetite Source: Ensembl
    6. neuron migration Source: UniProtKB
    7. positive regulation of apoptotic process Source: Ensembl
    8. positive regulation of cell proliferation Source: Ensembl
    9. positive regulation of mitochondrial depolarization Source: Ensembl
    10. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    11. positive regulation of protein oligomerization Source: Ensembl
    12. protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
    13. regulation of sensory perception of pain Source: Ensembl
    14. release of cytochrome c from mitochondria Source: Ensembl
    15. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Hormone

    Enzyme and pathway databases

    ReactomeiREACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cholecystokinin
    Short name:
    CCK
    Cleaved into the following 10 chains:
    Cholecystokinin-58
    Short name:
    CCK58
    Alternative name(s):
    (1-49)-CCK58
    Cholecystokinin-39
    Short name:
    CCK39
    Cholecystokinin-33
    Short name:
    CCK33
    Cholecystokinin-25
    Short name:
    CCK25
    Cholecystokinin-18
    Short name:
    CCK18
    Cholecystokinin-12
    Short name:
    CCK12
    Cholecystokinin-8
    Short name:
    CCK8
    Cholecystokinin-7
    Short name:
    CCK7
    Cholecystokinin-5
    Short name:
    CCK5
    Gene namesi
    Name:CCK
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:1569. CCK.

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: UniProtKB
    2. axon hillock Source: Ensembl
    3. axon initial segment Source: Ensembl
    4. dendrite Source: Ensembl
    5. extracellular region Source: Reactome
    6. extracellular space Source: Ensembl
    7. perikaryon Source: Ensembl
    8. terminal bouton Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi97 – 971Y → F: Reduces the quantity of secreted CCK8 by 50%. 1 Publication

    Organism-specific databases

    PharmGKBiPA26141.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Add
    BLAST
    Chaini21 – 11595CholecystokininPRO_0000010536Add
    BLAST
    Propeptidei21 – 4424PRO_0000010537Add
    BLAST
    Peptidei46 – 10358Cholecystokinin-58PRO_0000010538Add
    BLAST
    Peptidei46 – 9449Cholecystokinin-58 desnonopeptideBy similarityPRO_0000306304Add
    BLAST
    Peptidei65 – 10339Cholecystokinin-39PRO_0000010539Add
    BLAST
    Peptidei71 – 10333Cholecystokinin-33PRO_0000010540Add
    BLAST
    Peptidei79 – 10325Cholecystokinin-25By similarityPRO_0000306305Add
    BLAST
    Peptidei86 – 10318Cholecystokinin-18By similarityPRO_0000306306Add
    BLAST
    Peptidei92 – 10312Cholecystokinin-12PRO_0000010541Add
    BLAST
    Peptidei96 – 1038Cholecystokinin-8PRO_0000010542
    Peptidei97 – 1037Cholecystokinin-7By similarityPRO_0000306307
    Peptidei99 – 1035Cholecystokinin-5By similarityPRO_0000306308
    Propeptidei107 – 1159PRO_0000010543

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei97 – 971Sulfotyrosine1 Publication
    Modified residuei103 – 1031Phenylalanine amide1 Publication
    Modified residuei111 – 1111SulfotyrosineBy similarity
    Modified residuei113 – 1131SulfotyrosineBy similarity

    Post-translational modificationi

    The precursor is cleaved by proteases to produce a number of active cholecystokinins.

    Keywords - PTMi

    Amidation, Cleavage on pair of basic residues, Sulfation

    Proteomic databases

    PaxDbiP06307.
    PRIDEiP06307.

    PTM databases

    PhosphoSiteiP06307.

    Miscellaneous databases

    PMAP-CutDBP06307.

    Expressioni

    Gene expression databases

    ArrayExpressiP06307.
    BgeeiP06307.
    CleanExiHS_CCK.
    GenevestigatoriP06307.

    Organism-specific databases

    HPAiCAB002308.

    Interactioni

    Subunit structurei

    Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain.By similarity

    Protein-protein interaction databases

    BioGridi107327. 3 interactions.
    IntActiP06307. 1 interaction.
    STRINGi9606.ENSP00000335657.

    Structurei

    3D structure databases

    ProteinModelPortaliP06307.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the gastrin/cholecystokinin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40485.
    HOGENOMiHOG000073533.
    HOVERGENiHBG005806.
    InParanoidiP06307.
    KOiK05226.
    OMAiPSGRMSI.
    OrthoDBiEOG7F7WBS.
    PhylomeDBiP06307.
    TreeFamiTF333419.

    Family and domain databases

    InterProiIPR015499. CCK_like.
    IPR001651. Gastrin.
    IPR013152. Gastrin/cholecystokinin_CS.
    [Graphical view]
    PANTHERiPTHR10786. PTHR10786. 1 hit.
    PfamiPF00918. Gastrin. 1 hit.
    [Graphical view]
    SMARTiSM00029. GASTRIN. 1 hit.
    [Graphical view]
    PROSITEiPS00259. GASTRIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06307-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSGVCLCVL MAVLAAGALT QPVPPADPAG SGLQRAEEAP RRQLRVSQRT    50
    DGESRAHLGA LLARYIQQAR KAPSGRMSIV KNLQNLDPSH RISDRDYMGW 100
    MDFGRRSAEE YEYPS 115
    Length:115
    Mass (Da):12,669
    Last modified:January 1, 1988 - v1
    Checksum:i8A3EE6442FAEAF4B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti32 – 321G → E.1 Publication
    Corresponds to variant rs11571848 [ dbSNP | Ensembl ].
    VAR_018818
    Natural varianti95 – 951R → W.
    Corresponds to variant rs3774395 [ dbSNP | Ensembl ].
    VAR_024452

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00354 Genomic DNA. Translation: AAA53094.1.
    BT006991 mRNA. Translation: AAP35637.1.
    AY514491 Genomic DNA. Translation: AAR89908.1.
    BC008283 mRNA. Translation: AAH08283.1.
    CCDSiCCDS2696.1.
    PIRiA01623. GMHUCP.
    RefSeqiNP_000720.1. NM_000729.4.
    NP_001167609.1. NM_001174138.1.
    UniGeneiHs.458426.

    Genome annotation databases

    EnsembliENST00000334681; ENSP00000335657; ENSG00000187094.
    ENST00000396169; ENSP00000379472; ENSG00000187094.
    ENST00000434608; ENSP00000409124; ENSG00000187094.
    GeneIDi885.
    KEGGihsa:885.
    UCSCiuc003cld.1. human.

    Polymorphism databases

    DMDMi115945.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs
    Wikipedia

    Cholecystokinin entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00354 Genomic DNA. Translation: AAA53094.1 .
    BT006991 mRNA. Translation: AAP35637.1 .
    AY514491 Genomic DNA. Translation: AAR89908.1 .
    BC008283 mRNA. Translation: AAH08283.1 .
    CCDSi CCDS2696.1.
    PIRi A01623. GMHUCP.
    RefSeqi NP_000720.1. NM_000729.4.
    NP_001167609.1. NM_001174138.1.
    UniGenei Hs.458426.

    3D structure databases

    ProteinModelPortali P06307.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107327. 3 interactions.
    IntActi P06307. 1 interaction.
    STRINGi 9606.ENSP00000335657.

    Chemistry

    BindingDBi P06307.
    ChEMBLi CHEMBL1649050.

    PTM databases

    PhosphoSitei P06307.

    Polymorphism databases

    DMDMi 115945.

    Proteomic databases

    PaxDbi P06307.
    PRIDEi P06307.

    Protocols and materials databases

    DNASUi 885.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000334681 ; ENSP00000335657 ; ENSG00000187094 .
    ENST00000396169 ; ENSP00000379472 ; ENSG00000187094 .
    ENST00000434608 ; ENSP00000409124 ; ENSG00000187094 .
    GeneIDi 885.
    KEGGi hsa:885.
    UCSCi uc003cld.1. human.

    Organism-specific databases

    CTDi 885.
    GeneCardsi GC03M042274.
    HGNCi HGNC:1569. CCK.
    HPAi CAB002308.
    MIMi 118440. gene.
    neXtProti NX_P06307.
    PharmGKBi PA26141.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG40485.
    HOGENOMi HOG000073533.
    HOVERGENi HBG005806.
    InParanoidi P06307.
    KOi K05226.
    OMAi PSGRMSI.
    OrthoDBi EOG7F7WBS.
    PhylomeDBi P06307.
    TreeFami TF333419.

    Enzyme and pathway databases

    Reactomei REACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.

    Miscellaneous databases

    ChiTaRSi CCK. human.
    GeneWikii Cholecystokinin.
    GenomeRNAii 885.
    NextBioi 3658.
    PMAP-CutDB P06307.
    PROi P06307.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P06307.
    Bgeei P06307.
    CleanExi HS_CCK.
    Genevestigatori P06307.

    Family and domain databases

    InterProi IPR015499. CCK_like.
    IPR001651. Gastrin.
    IPR013152. Gastrin/cholecystokinin_CS.
    [Graphical view ]
    PANTHERi PTHR10786. PTHR10786. 1 hit.
    Pfami PF00918. Gastrin. 1 hit.
    [Graphical view ]
    SMARTi SM00029. GASTRIN. 1 hit.
    [Graphical view ]
    PROSITEi PS00259. GASTRIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the human cholecystokinin gene."
      Kato K., Takahashi Y., Matsubara K.
      Ann. N. Y. Acad. Sci. 448:613-615(1985)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine."
      Takahashi Y., Kato K., Hayashizaki Y., Wakabayashi T., Ohtsuka E., Matsuki S., Ikehara M., Matsubara K.
      Proc. Natl. Acad. Sci. U.S.A. 82:1931-1935(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], AMIDATION AT PHE-103.
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. NIEHS SNPs program
      Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-32.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas.
    6. "Role of tyrosine sulfation and serine phosphorylation in the processing of procholecystokinin to amidated cholecystokinin and its secretion in transfected AtT-20 cells."
      Vishnuvardhan D., Beinfeld M.C.
      Biochemistry 39:13825-13830(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF TYR-97, SULFATION AT TYR-97.

    Entry informationi

    Entry nameiCCKN_HUMAN
    AccessioniPrimary (citable) accession number: P06307
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3