P06307 (CCKN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholecystokinin
Short name=CCK

Cleaved into the following 10 chains:

1. Cholecystokinin-58
   Short name=CCK58
2. Cholecystokinin-58 desnonopeptide
   Alternative name(s):
   (1-49)-CCK58
3. Cholecystokinin-39
   Short name=CCK39
4. Cholecystokinin-33
   Short name=CCK33
5. Cholecystokinin-25
   Short name=CCK25
6. Cholecystokinin-18
   Short name=CCK18
7. Cholecystokinin-12
   Short name=CCK12
8. Cholecystokinin-8
   Short name=CCK8
9. Cholecystokinin-7
   Short name=CCK7
10. Cholecystokinin-5
    Short name=CCK5

Gene names

Name:

CCK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
Subunit structure	Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain By similarity.
Subcellular location	Secreted.
Post-translational modification	The precursor is cleaved by proteases to produce a number of active cholecystokinins.
Sequence similarities	Belongs to the gastrin/cholecystokinin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Sulfation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	axonogenesis Inferred from sequence or structural similarity. Source: UniProtKB eating behavior Inferred from sequence or structural similarity. Source: UniProtKB neuron migration Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	neuropeptide hormone activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 115	95	Cholecystokinin		PRO_0000010536
	Propeptide	21 – 44	24			PRO_0000010537
	Peptide	46 – 103	58	Cholecystokinin-58		PRO_0000010538
	Peptide	46 – 94	49	Cholecystokinin-58 desnonopeptide By similarity		PRO_0000306304
	Peptide	65 – 103	39	Cholecystokinin-39		PRO_0000010539
	Peptide	71 – 103	33	Cholecystokinin-33		PRO_0000010540
	Peptide	79 – 103	25	Cholecystokinin-25 By similarity		PRO_0000306305
	Peptide	86 – 103	18	Cholecystokinin-18 By similarity		PRO_0000306306
	Peptide	92 – 103	12	Cholecystokinin-12		PRO_0000010541
	Peptide	96 – 103	8	Cholecystokinin-8		PRO_0000010542
	Peptide	97 – 103	7	Cholecystokinin-7 By similarity		PRO_0000306307
	Peptide	99 – 103	5	Cholecystokinin-5 By similarity		PRO_0000306308
	Propeptide	107 – 115	9			PRO_0000010543
Amino acid modifications
	Modified residue	97	1	Sulfotyrosine
	Modified residue	103	1	Phenylalanine amide
	Modified residue	111	1	Sulfotyrosine By similarity
	Modified residue	113	1	Sulfotyrosine By similarity
Natural variations
	Natural variant	32	1	G → E. Ref.4 Corresponds to variant rs11571848 [ dbSNP | Ensembl ].		VAR_018818
	Natural variant	95	1	R → W. Corresponds to variant rs3774395 [ dbSNP | Ensembl ].		VAR_024452
Experimental info
	Mutagenesis	97	1	Y → F: Reduces the quantity of secreted CCK8 by 50%. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P06307 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 8A3EE6442FAEAF4B	FASTA	115	12,669
	10 20 30 40 50 60 MNSGVCLCVL MAVLAAGALT QPVPPADPAG SGLQRAEEAP RRQLRVSQRT DGESRAHLGA 70 80 90 100 110 LLARYIQQAR KAPSGRMSIV KNLQNLDPSH RISDRDYMGW MDFGRRSAEE YEYPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the human cholecystokinin gene." Kato K., Takahashi Y., Matsubara K. Ann. N. Y. Acad. Sci. 448:613-615(1985) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine." Takahashi Y., Kato K., Hayashizaki Y., Wakabayashi T., Ohtsuka E., Matsuki S., Ikehara M., Matsubara K. Proc. Natl. Acad. Sci. U.S.A. 82:1931-1935(1985) [PubMed: 3856870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	NIEHS SNPs program Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-32.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[6]	"Role of tyrosine sulfation and serine phosphorylation in the processing of procholecystokinin to amidated cholecystokinin and its secretion in transfected AtT-20 cells." Vishnuvardhan D., Beinfeld M.C. Biochemistry 39:13825-13830(2000) [PubMed: 11076522] [Abstract] Cited for: MUTAGENESIS OF TYR-97.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Wikipedia

Cholecystokinin entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	L00354 Genomic DNA. Translation: AAA53094.1. BT006991 mRNA. Translation: AAP35637.1. AY514491 Genomic DNA. Translation: AAR89908.1. BC008283 mRNA. Translation: AAH08283.1.
IPI	IPI00026174.
PIR	GMHUCP. A01623.
RefSeq	NP_000720.1. NM_000729.4. NP_001167609.1. NM_001174138.1.
UniGene	Hs.458426.
3D structure databases
ProteinModelPortal	P06307.
ModBase	Search...
Protein-protein interaction databases
STRING	P06307.
Polymorphism databases
DMDM	115945.
Proteomic databases
PRIDE	P06307.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334681; ENSP00000335657; ENSG00000187094. ENST00000396169; ENSP00000379472; ENSG00000187094. ENST00000434608; ENSP00000409124; ENSG00000187094.
GeneID	885.
KEGG	hsa:885.
UCSC	uc003clc.1. human.
Organism-specific databases
CTD	885.
GeneCards	GC03M042274.
H-InvDB	HIX0003200.
HGNC	HGNC:1569. CCK.
HPA	CAB002308.
MIM	118440. gene.
neXtProt	NX_P06307.
PharmGKB	PA26141.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20670.
HOGENOM	HBG444941.
HOVERGEN	HBG005806.
InParanoid	P06307.
OMA	QRTDGES.
OrthoDB	EOG4Z8XXW.
PhylomeDB	P06307.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P06307.
Bgee	P06307.
CleanEx	HS_CCK.
Genevestigator	P06307.
GermOnline	ENSG00000187094. Homo sapiens.
Family and domain databases
InterPro	IPR015499. CCK. IPR001651. Gastrin. IPR013152. Gastrin/cholecystokinin_CS. [Graphical view]
KO	K05226.
PANTHER	PTHR10786. CCK. 1 hit.
Pfam	PF00918. Gastrin. 1 hit. [Graphical view]
SMART	SM00029. GASTRIN. 1 hit. [Graphical view]
PROSITE	PS00259. GASTRIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	3658.
PMAP-CutDB	P06307.
SOURCE	Search...

Entry information

Entry name

CCKN_HUMAN

Accession

Primary (citable) accession number: P06307

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	December 14, 2011
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.