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P06307 (CCKN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholecystokinin

Short name=CCK

Cleaved into the following 10 chains:

  1. Cholecystokinin-58
    Short name=CCK58
  2. Cholecystokinin-58 desnonopeptide
    Alternative name(s):
    (1-49)-CCK58
  3. Cholecystokinin-39
    Short name=CCK39
  4. Cholecystokinin-33
    Short name=CCK33
  5. Cholecystokinin-25
    Short name=CCK25
  6. Cholecystokinin-18
    Short name=CCK18
  7. Cholecystokinin-12
    Short name=CCK12
  8. Cholecystokinin-8
    Short name=CCK8
  9. Cholecystokinin-7
    Short name=CCK7
  10. Cholecystokinin-5
    Short name=CCK5
Gene names
Name:CCK
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length115 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.

Subunit structure

Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain By similarity.

Subcellular location

Secreted.

Post-translational modification

The precursor is cleaved by proteases to produce a number of active cholecystokinins.

Sequence similarities

Belongs to the gastrin/cholecystokinin family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionHormone
   PTMAmidation
Cleavage on pair of basic residues
Sulfation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from electronic annotation. Source: Ensembl

axonogenesis

Inferred from sequence or structural similarity PubMed 15152034. Source: UniProtKB

behavioral fear response

Inferred from electronic annotation. Source: Ensembl

eating behavior

Inferred from sequence or structural similarity PubMed 15374756. Source: UniProtKB

negative regulation of appetite

Inferred from electronic annotation. Source: Ensembl

neuron migration

Inferred from sequence or structural similarity PubMed 15152034. Source: UniProtKB

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of mitochondrial depolarization

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein oligomerization

Inferred from electronic annotation. Source: Ensembl

protein kinase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

regulation of sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

release of cytochrome c from mitochondria

Inferred from electronic annotation. Source: Ensembl

signal transduction

Traceable author statement PubMed 10320330. Source: ProtInc

   Cellular_componentaxon

Inferred from sequence or structural similarity PubMed 15152034. Source: UniProtKB

axon hillock

Inferred from electronic annotation. Source: Ensembl

axon initial segment

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from electronic annotation. Source: Ensembl

extracellular region

Traceable author statement. Source: Reactome

extracellular space

Inferred from electronic annotation. Source: Ensembl

perikaryon

Inferred from electronic annotation. Source: Ensembl

terminal bouton

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionhormone activity

Traceable author statement PubMed 10320330. Source: ProtInc

neuropeptide hormone activity

Inferred from sequence or structural similarity PubMed 15374756. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 11595Cholecystokinin
PRO_0000010536
Propeptide21 – 4424
PRO_0000010537
Peptide46 – 10358Cholecystokinin-58
PRO_0000010538
Peptide46 – 9449Cholecystokinin-58 desnonopeptide By similarity
PRO_0000306304
Peptide65 – 10339Cholecystokinin-39
PRO_0000010539
Peptide71 – 10333Cholecystokinin-33
PRO_0000010540
Peptide79 – 10325Cholecystokinin-25 By similarity
PRO_0000306305
Peptide86 – 10318Cholecystokinin-18 By similarity
PRO_0000306306
Peptide92 – 10312Cholecystokinin-12
PRO_0000010541
Peptide96 – 1038Cholecystokinin-8
PRO_0000010542
Peptide97 – 1037Cholecystokinin-7 By similarity
PRO_0000306307
Peptide99 – 1035Cholecystokinin-5 By similarity
PRO_0000306308
Propeptide107 – 1159
PRO_0000010543

Amino acid modifications

Modified residue971Sulfotyrosine
Modified residue1031Phenylalanine amide
Modified residue1111Sulfotyrosine By similarity
Modified residue1131Sulfotyrosine By similarity

Natural variations

Natural variant321G → E. Ref.4
Corresponds to variant rs11571848 [ dbSNP | Ensembl ].
VAR_018818
Natural variant951R → W.
Corresponds to variant rs3774395 [ dbSNP | Ensembl ].
VAR_024452

Experimental info

Mutagenesis971Y → F: Reduces the quantity of secreted CCK8 by 50%. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P06307 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 8A3EE6442FAEAF4B

FASTA11512,669
        10         20         30         40         50         60 
MNSGVCLCVL MAVLAAGALT QPVPPADPAG SGLQRAEEAP RRQLRVSQRT DGESRAHLGA 

        70         80         90        100        110 
LLARYIQQAR KAPSGRMSIV KNLQNLDPSH RISDRDYMGW MDFGRRSAEE YEYPS 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of the human cholecystokinin gene."
Kato K., Takahashi Y., Matsubara K.
Ann. N. Y. Acad. Sci. 448:613-615(1985)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine."
Takahashi Y., Kato K., Hayashizaki Y., Wakabayashi T., Ohtsuka E., Matsuki S., Ikehara M., Matsubara K.
Proc. Natl. Acad. Sci. U.S.A. 82:1931-1935(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]NIEHS SNPs program
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-32.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[6]"Role of tyrosine sulfation and serine phosphorylation in the processing of procholecystokinin to amidated cholecystokinin and its secretion in transfected AtT-20 cells."
Vishnuvardhan D., Beinfeld M.C.
Biochemistry 39:13825-13830(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF TYR-97.
+Additional computationally mapped references.

Web resources

NIEHS-SNPs
Wikipedia

Cholecystokinin entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L00354 Genomic DNA. Translation: AAA53094.1.
BT006991 mRNA. Translation: AAP35637.1.
AY514491 Genomic DNA. Translation: AAR89908.1.
BC008283 mRNA. Translation: AAH08283.1.
PIRGMHUCP. A01623.
RefSeqNP_000720.1. NM_000729.4.
NP_001167609.1. NM_001174138.1.
UniGeneHs.458426.

3D structure databases

ProteinModelPortalP06307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107327. 3 interactions.
IntActP06307. 1 interaction.
STRING9606.ENSP00000335657.

Chemistry

BindingDBP06307.
ChEMBLCHEMBL1649050.

PTM databases

PhosphoSiteP06307.

Polymorphism databases

DMDM115945.

Proteomic databases

PaxDbP06307.
PRIDEP06307.

Protocols and materials databases

DNASU885.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000334681; ENSP00000335657; ENSG00000187094.
ENST00000396169; ENSP00000379472; ENSG00000187094.
ENST00000434608; ENSP00000409124; ENSG00000187094.
GeneID885.
KEGGhsa:885.
UCSCuc003cld.1. human.

Organism-specific databases

CTD885.
GeneCardsGC03M042274.
HGNCHGNC:1569. CCK.
HPACAB002308.
MIM118440. gene.
neXtProtNX_P06307.
PharmGKBPA26141.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40485.
HOGENOMHOG000073533.
HOVERGENHBG005806.
InParanoidP06307.
KOK05226.
OMAPSGRMSI.
OrthoDBEOG7F7WBS.
PhylomeDBP06307.
TreeFamTF333419.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP06307.
BgeeP06307.
CleanExHS_CCK.
GenevestigatorP06307.

Family and domain databases

InterProIPR015499. CCK.
IPR001651. Gastrin.
IPR013152. Gastrin/cholecystokinin_CS.
[Graphical view]
PANTHERPTHR10786. PTHR10786. 1 hit.
PfamPF00918. Gastrin. 1 hit.
[Graphical view]
SMARTSM00029. GASTRIN. 1 hit.
[Graphical view]
PROSITEPS00259. GASTRIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCCK. human.
GeneWikiCholecystokinin.
GenomeRNAi885.
NextBio3658.
PMAP-CutDBP06307.
PROP06307.
SOURCESearch...

Entry information

Entry nameCCKN_HUMAN
AccessionPrimary (citable) accession number: P06307
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM