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Protein

Cholecystokinin

Gene

CCK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.

GO - Molecular functioni

  1. hormone activity Source: ProtInc
  2. neuropeptide hormone activity Source: UniProtKB

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  2. axonogenesis Source: UniProtKB
  3. behavioral fear response Source: Ensembl
  4. eating behavior Source: UniProtKB
  5. negative regulation of appetite Source: Ensembl
  6. neuron migration Source: UniProtKB
  7. positive regulation of cell proliferation Source: Ensembl
  8. positive regulation of mitochondrial depolarization Source: Ensembl
  9. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  10. positive regulation of protein oligomerization Source: Ensembl
  11. protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  12. regulation of sensory perception of pain Source: Ensembl
  13. release of cytochrome c from mitochondria Source: Ensembl
  14. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiREACT_14819. Peptide ligand-binding receptors.
REACT_18283. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholecystokinin
Short name:
CCK
Cleaved into the following 10 chains:
Cholecystokinin-58
Short name:
CCK58
Alternative name(s):
(1-49)-CCK58
Cholecystokinin-39
Short name:
CCK39
Cholecystokinin-33
Short name:
CCK33
Cholecystokinin-25
Short name:
CCK25
Cholecystokinin-18
Short name:
CCK18
Cholecystokinin-12
Short name:
CCK12
Cholecystokinin-8
Short name:
CCK8
Cholecystokinin-7
Short name:
CCK7
Cholecystokinin-5
Short name:
CCK5
Gene namesi
Name:CCK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:1569. CCK.

Subcellular locationi

GO - Cellular componenti

  1. axon Source: UniProtKB
  2. axon hillock Source: Ensembl
  3. axon initial segment Source: Ensembl
  4. dendrite Source: Ensembl
  5. extracellular region Source: Reactome
  6. extracellular space Source: Ensembl
  7. perikaryon Source: Ensembl
  8. terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi97 – 971Y → F: Reduces the quantity of secreted CCK8 by 50%. 1 Publication

Organism-specific databases

PharmGKBiPA26141.

Polymorphism and mutation databases

BioMutaiCCK.
DMDMi115945.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 11595CholecystokininPRO_0000010536Add
BLAST
Propeptidei21 – 4424PRO_0000010537Add
BLAST
Peptidei46 – 10358Cholecystokinin-58PRO_0000010538Add
BLAST
Peptidei46 – 9449Cholecystokinin-58 desnonopeptideBy similarityPRO_0000306304Add
BLAST
Peptidei65 – 10339Cholecystokinin-39PRO_0000010539Add
BLAST
Peptidei71 – 10333Cholecystokinin-33PRO_0000010540Add
BLAST
Peptidei79 – 10325Cholecystokinin-25By similarityPRO_0000306305Add
BLAST
Peptidei86 – 10318Cholecystokinin-18By similarityPRO_0000306306Add
BLAST
Peptidei92 – 10312Cholecystokinin-12PRO_0000010541Add
BLAST
Peptidei96 – 1038Cholecystokinin-8PRO_0000010542
Peptidei97 – 1037Cholecystokinin-7By similarityPRO_0000306307
Peptidei99 – 1035Cholecystokinin-5By similarityPRO_0000306308
Propeptidei107 – 1159PRO_0000010543

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei97 – 971Sulfotyrosine1 Publication
Modified residuei103 – 1031Phenylalanine amide1 Publication
Modified residuei111 – 1111SulfotyrosineBy similarity
Modified residuei113 – 1131SulfotyrosineBy similarity

Post-translational modificationi

The precursor is cleaved by proteases to produce a number of active cholecystokinins.

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Sulfation

Proteomic databases

PaxDbiP06307.
PRIDEiP06307.

PTM databases

PhosphoSiteiP06307.

Miscellaneous databases

PMAP-CutDBP06307.

Expressioni

Gene expression databases

BgeeiP06307.
CleanExiHS_CCK.
ExpressionAtlasiP06307. baseline and differential.
GenevestigatoriP06307.

Organism-specific databases

HPAiCAB002308.

Interactioni

Subunit structurei

Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain.By similarity

Protein-protein interaction databases

BioGridi107327. 3 interactions.
IntActiP06307. 1 interaction.
STRINGi9606.ENSP00000335657.

Structurei

3D structure databases

ProteinModelPortaliP06307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gastrin/cholecystokinin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40485.
HOGENOMiHOG000073533.
HOVERGENiHBG005806.
InParanoidiP06307.
KOiK05226.
OMAiGRMSIVK.
OrthoDBiEOG7F7WBS.
PhylomeDBiP06307.
TreeFamiTF333419.

Family and domain databases

InterProiIPR015499. CCK_like.
IPR001651. Gastrin.
IPR013152. Gastrin/cholecystokinin_CS.
[Graphical view]
PANTHERiPTHR10786. PTHR10786. 1 hit.
PfamiPF00918. Gastrin. 1 hit.
[Graphical view]
SMARTiSM00029. GASTRIN. 1 hit.
[Graphical view]
PROSITEiPS00259. GASTRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSGVCLCVL MAVLAAGALT QPVPPADPAG SGLQRAEEAP RRQLRVSQRT
60 70 80 90 100
DGESRAHLGA LLARYIQQAR KAPSGRMSIV KNLQNLDPSH RISDRDYMGW
110
MDFGRRSAEE YEYPS
Length:115
Mass (Da):12,669
Last modified:January 1, 1988 - v1
Checksum:i8A3EE6442FAEAF4B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti32 – 321G → E.1 Publication
Corresponds to variant rs11571848 [ dbSNP | Ensembl ].
VAR_018818
Natural varianti95 – 951R → W.
Corresponds to variant rs3774395 [ dbSNP | Ensembl ].
VAR_024452

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00354 Genomic DNA. Translation: AAA53094.1.
BT006991 mRNA. Translation: AAP35637.1.
AY514491 Genomic DNA. Translation: AAR89908.1.
BC008283 mRNA. Translation: AAH08283.1.
CCDSiCCDS2696.1.
PIRiA01623. GMHUCP.
RefSeqiNP_000720.1. NM_000729.4.
NP_001167609.1. NM_001174138.1.
UniGeneiHs.458426.

Genome annotation databases

EnsembliENST00000334681; ENSP00000335657; ENSG00000187094.
ENST00000396169; ENSP00000379472; ENSG00000187094.
ENST00000434608; ENSP00000409124; ENSG00000187094.
GeneIDi885.
KEGGihsa:885.
UCSCiuc003cld.1. human.

Polymorphism and mutation databases

BioMutaiCCK.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs
Wikipedia

Cholecystokinin entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00354 Genomic DNA. Translation: AAA53094.1.
BT006991 mRNA. Translation: AAP35637.1.
AY514491 Genomic DNA. Translation: AAR89908.1.
BC008283 mRNA. Translation: AAH08283.1.
CCDSiCCDS2696.1.
PIRiA01623. GMHUCP.
RefSeqiNP_000720.1. NM_000729.4.
NP_001167609.1. NM_001174138.1.
UniGeneiHs.458426.

3D structure databases

ProteinModelPortaliP06307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107327. 3 interactions.
IntActiP06307. 1 interaction.
STRINGi9606.ENSP00000335657.

Chemistry

BindingDBiP06307.
ChEMBLiCHEMBL1649050.

PTM databases

PhosphoSiteiP06307.

Polymorphism and mutation databases

BioMutaiCCK.
DMDMi115945.

Proteomic databases

PaxDbiP06307.
PRIDEiP06307.

Protocols and materials databases

DNASUi885.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334681; ENSP00000335657; ENSG00000187094.
ENST00000396169; ENSP00000379472; ENSG00000187094.
ENST00000434608; ENSP00000409124; ENSG00000187094.
GeneIDi885.
KEGGihsa:885.
UCSCiuc003cld.1. human.

Organism-specific databases

CTDi885.
GeneCardsiGC03M042274.
HGNCiHGNC:1569. CCK.
HPAiCAB002308.
MIMi118440. gene.
neXtProtiNX_P06307.
PharmGKBiPA26141.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG40485.
HOGENOMiHOG000073533.
HOVERGENiHBG005806.
InParanoidiP06307.
KOiK05226.
OMAiGRMSIVK.
OrthoDBiEOG7F7WBS.
PhylomeDBiP06307.
TreeFamiTF333419.

Enzyme and pathway databases

ReactomeiREACT_14819. Peptide ligand-binding receptors.
REACT_18283. G alpha (q) signalling events.

Miscellaneous databases

ChiTaRSiCCK. human.
GeneWikiiCholecystokinin.
GenomeRNAii885.
NextBioi3658.
PMAP-CutDBP06307.
PROiP06307.
SOURCEiSearch...

Gene expression databases

BgeeiP06307.
CleanExiHS_CCK.
ExpressionAtlasiP06307. baseline and differential.
GenevestigatoriP06307.

Family and domain databases

InterProiIPR015499. CCK_like.
IPR001651. Gastrin.
IPR013152. Gastrin/cholecystokinin_CS.
[Graphical view]
PANTHERiPTHR10786. PTHR10786. 1 hit.
PfamiPF00918. Gastrin. 1 hit.
[Graphical view]
SMARTiSM00029. GASTRIN. 1 hit.
[Graphical view]
PROSITEiPS00259. GASTRIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the human cholecystokinin gene."
    Kato K., Takahashi Y., Matsubara K.
    Ann. N. Y. Acad. Sci. 448:613-615(1985)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine."
    Takahashi Y., Kato K., Hayashizaki Y., Wakabayashi T., Ohtsuka E., Matsuki S., Ikehara M., Matsubara K.
    Proc. Natl. Acad. Sci. U.S.A. 82:1931-1935(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], AMIDATION AT PHE-103.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. NIEHS SNPs program
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-32.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  6. "Role of tyrosine sulfation and serine phosphorylation in the processing of procholecystokinin to amidated cholecystokinin and its secretion in transfected AtT-20 cells."
    Vishnuvardhan D., Beinfeld M.C.
    Biochemistry 39:13825-13830(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF TYR-97, SULFATION AT TYR-97.

Entry informationi

Entry nameiCCKN_HUMAN
AccessioniPrimary (citable) accession number: P06307
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 29, 2015
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.