Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P06281

- RENI1_MOUSE

UniProt

P06281 - RENI1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Renin-1

Gene

Ren1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Catalytic activityi

Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Enzyme regulationi

Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021PROSITE-ProRule annotation
Active sitei287 – 2871PROSITE-ProRule annotation

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. endopeptidase activity Source: MGI

GO - Biological processi

  1. angiotensin maturation Source: MGI
  2. beta-amyloid metabolic process Source: Ensembl
  3. cell maturation Source: MGI
  4. cellular response to drug Source: Ensembl
  5. drinking behavior Source: MGI
  6. hormone-mediated signaling pathway Source: MGI
  7. male gonad development Source: UniProtKB
  8. mesonephros development Source: UniProtKB
  9. regulation of blood volume by renin-angiotensin Source: MGI
  10. regulation of MAPK cascade Source: Ensembl
  11. renin-angiotensin regulation of aldosterone production Source: MGI
  12. response to cAMP Source: Ensembl
  13. response to cGMP Source: Ensembl
  14. response to lipopolysaccharide Source: Ensembl
  15. response to organic substance Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiREACT_196539. Metabolism of Angiotensinogen to Angiotensins.

Protein family/group databases

MEROPSiA01.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Renin-1 (EC:3.4.23.15)
Alternative name(s):
Angiotensinogenase
Kidney renin
Gene namesi
Name:Ren1
Synonyms:Ren, Ren-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:97898. Ren1.

Subcellular locationi

Secreted By similarity. Membrane By similarity
Note: Associated to membranes via binding to ATP6AP2.By similarity

GO - Cellular componenti

  1. extracellular space Source: MGI
  2. intracellular Source: MGI
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Propeptidei22 – 7150Activation peptide1 PublicationPRO_0000026089Add
BLAST
Chaini72 – 402331Renin-1PRO_0000026090Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi115 ↔ 122By similarity
Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi278 ↔ 282By similarity
Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP06281.
PaxDbiP06281.
PRIDEiP06281.

PTM databases

PhosphoSiteiP06281.

Expressioni

Tissue specificityi

Kidney.

Inductioni

Renal renin is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration.

Gene expression databases

BgeeiP06281.
CleanExiMM_REN1.
ExpressionAtlasiP06281. baseline and differential.
GenevestigatoriP06281.

Interactioni

Subunit structurei

Interacts with ATP6AP2.By similarity

Protein-protein interaction databases

IntActiP06281. 1 interaction.
MINTiMINT-4132153.

Structurei

3D structure databases

ProteinModelPortaliP06281.
SMRiP06281. Positions 28-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG248684.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP06281.
KOiK01380.
OMAiVVCEEGC.
OrthoDBiEOG7HQN88.
PhylomeDBiP06281.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06281-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDRRRMPLWA LLLLWSPCTF SLPTRTATFE RIPLKKMPSV REILEERGVD
60 70 80 90 100
MTRLSAEWGV FTKRPSLTNL TSPVVLTNYL NTQYYGEIGI GTPPQTFKVI
110 120 130 140 150
FDTGSANLWV PSTKCSRLYL ACGIHSLYES SDSSSYMENG SDFTIHYGSG
160 170 180 190 200
RVKGFLSQDS VTVGGITVTQ TFGEVTELPL IPFMLAKFDG VLGMGFPAQA
210 220 230 240 250
VGGVTPVFDH ILSQGVLKEE VFSVYYNRGS HLLGGEVVLG GSDPQHYQGN
260 270 280 290 300
FHYVSISKTD SWQITMKGVS VGSSTLLCEE GCAVVVDTGS SFISAPTSSL
310 320 330 340 350
KLIMQALGAK EKRIEEYVVN CSQVPTLPDI SFDLGGRAYT LSSTDYVLQY
360 370 380 390 400
PNRRDKLCTL ALHAMDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL

AR
Length:402
Mass (Da):44,343
Last modified:January 1, 1988 - v1
Checksum:iD42920B555E97A38
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 2318Missing(PubMed:6370686)CuratedAdd
BLAST
Sequence conflicti24 – 241T → I(PubMed:6370686)Curated
Sequence conflicti163 – 1631V → VSRV(PubMed:6370686)Curated

Polymorphismi

In inbred mouse strains, there are at least two alleles which can occur at the Ren1 locus: Ren-1D and Ren-1C. The sequence shown is that of Ren-1C.
Present as a single-copy gene in strains such as BALB/c and C57BL/6 while some strains such as Swiss and Akr contain two copies.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti58 – 581W → R in allele Ren-1D.
Natural varianti68 – 681T → I in allele Ren-1D.
Natural varianti160 – 1601S → V in allele Ren-1D.
Natural varianti315 – 3151E → D in allele Ren-1D. 1 Publication
Natural varianti352 – 3521N → Y in allele Ren-1D.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00810
, X00811, X00812, X00813, X00814, X00815, X00816, X00850, X00851 Genomic DNA. Translation: CAA25391.1.
X16642 mRNA. Translation: CAA34636.1.
K02596 Genomic DNA. Translation: AAA40045.1.
M32352 Genomic DNA. Translation: AAA40043.1.
AK052685 mRNA. Translation: BAC35094.1.
AK085309 mRNA. Translation: BAC39418.1.
BC061053 mRNA. Translation: AAH61053.1.
K02800 Genomic DNA. Translation: AAA40044.1.
M34190 Genomic DNA. Translation: AAA40042.1.
CCDSiCCDS15295.1.
PIRiA00989. REMSK.
RefSeqiNP_112469.1. NM_031192.3.
UniGeneiMm.220955.

Genome annotation databases

EnsembliENSMUST00000094556; ENSMUSP00000092135; ENSMUSG00000070645.
GeneIDi19701.
KEGGimmu:19701.
UCSCiuc007cqf.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00810
, X00811 , X00812 , X00813 , X00814 , X00815 , X00816 , X00850 , X00851 Genomic DNA. Translation: CAA25391.1 .
X16642 mRNA. Translation: CAA34636.1 .
K02596 Genomic DNA. Translation: AAA40045.1 .
M32352 Genomic DNA. Translation: AAA40043.1 .
AK052685 mRNA. Translation: BAC35094.1 .
AK085309 mRNA. Translation: BAC39418.1 .
BC061053 mRNA. Translation: AAH61053.1 .
K02800 Genomic DNA. Translation: AAA40044.1 .
M34190 Genomic DNA. Translation: AAA40042.1 .
CCDSi CCDS15295.1.
PIRi A00989. REMSK.
RefSeqi NP_112469.1. NM_031192.3.
UniGenei Mm.220955.

3D structure databases

ProteinModelPortali P06281.
SMRi P06281. Positions 28-402.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P06281. 1 interaction.
MINTi MINT-4132153.

Chemistry

BindingDBi P06281.
ChEMBLi CHEMBL2615.

Protein family/group databases

MEROPSi A01.007.

PTM databases

PhosphoSitei P06281.

Proteomic databases

MaxQBi P06281.
PaxDbi P06281.
PRIDEi P06281.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000094556 ; ENSMUSP00000092135 ; ENSMUSG00000070645 .
GeneIDi 19701.
KEGGi mmu:19701.
UCSCi uc007cqf.1. mouse.

Organism-specific databases

CTDi 19701.
MGIi MGI:97898. Ren1.

Phylogenomic databases

eggNOGi NOG248684.
GeneTreei ENSGT00760000118929.
HOGENOMi HOG000197681.
HOVERGENi HBG000482.
InParanoidi P06281.
KOi K01380.
OMAi VVCEEGC.
OrthoDBi EOG7HQN88.
PhylomeDBi P06281.
TreeFami TF314990.

Enzyme and pathway databases

Reactomei REACT_196539. Metabolism of Angiotensinogen to Angiotensins.

Miscellaneous databases

NextBioi 297064.
PROi P06281.
SOURCEi Search...

Gene expression databases

Bgeei P06281.
CleanExi MM_REN1.
ExpressionAtlasi P06281. baseline and differential.
Genevestigatori P06281.

Family and domain databases

Gene3Di 2.40.70.10. 2 hits.
InterProi IPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view ]
PANTHERi PTHR13683. PTHR13683. 1 hit.
Pfami PF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view ]
PRINTSi PR00792. PEPSIN.
SUPFAMi SSF50630. SSF50630. 1 hit.
PROSITEi PS00141. ASP_PROTEASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Evolution of aspartyl proteases by gene duplication: the mouse renin gene is organized in two homologous clusters of four exons."
    Holm I., Ollo R., Panthier J.-J., Rougeon F.
    EMBO J. 3:557-562(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
  2. "Nucleotide sequence of a cDNA coding for mouse Ren1 preprorenin."
    Kim W.S., Murakami K., Nakayama K.
    Nucleic Acids Res. 17:9480-9480(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Kidney.
  3. "The nucleotide sequence of a mouse renin-encoding gene, Ren-1d, and its upstream region."
    Burt D.W., Mullins L.J., George H., Smith G., Brooks J., Pioli D., Brammar W.J.
    Gene 84:91-104(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/10 and DBA/2.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  6. "Mouse kidney and submaxillary gland renin genes differ in their 5' putative regulatory sequences."
    Panthier J.-J., Dreyfus M., Roux D.T.L., Rougeon F.
    Proc. Natl. Acad. Sci. U.S.A. 81:5489-5493(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
    Tissue: Kidney.
  7. "Expression of tissue-specific Ren-1 and Ren-2 genes of mice: comparative analysis of 5'-proximal flanking regions."
    Field L.J., Philbrick W.M., Howles P.N., Dickinson D.P., McGowan R.A., Gross K.W.
    Mol. Cell. Biol. 4:2321-2331(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
  8. Cited for: PROTEIN SEQUENCE OF 22-37 AND 72-80.
    Strain: C57BL/10ROS X C3H/HEROS.
    Tissue: Kidney.
  9. "Molecular cloning of two distinct renin genes from the DBA/2 mouse."
    Mullins J.J., Burt D.W., Windass J.D., McTurk P., George H., Brammar W.J.
    EMBO J. 1:1461-1466(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 269-316, VARIANT ASP-315.
    Strain: DBA/2.
    Tissue: Submandibular gland.

Entry informationi

Entry nameiRENI1_MOUSE
AccessioniPrimary (citable) accession number: P06281
Secondary accession number(s): P97911
, Q543E5, Q62153, Q62154
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 29, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3