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P06281

- RENI1_MOUSE

UniProt

P06281 - RENI1_MOUSE

Protein

Renin-1

Gene

Ren1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

    Catalytic activityi

    Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

    Enzyme regulationi

    Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei102 – 1021PROSITE-ProRule annotation
    Active sitei287 – 2871PROSITE-ProRule annotation

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW
    2. endopeptidase activity Source: MGI

    GO - Biological processi

    1. angiotensin maturation Source: MGI
    2. beta-amyloid metabolic process Source: Ensembl
    3. cell maturation Source: MGI
    4. cellular response to drug Source: Ensembl
    5. drinking behavior Source: MGI
    6. hormone-mediated signaling pathway Source: MGI
    7. male gonad development Source: UniProtKB
    8. mesonephros development Source: UniProtKB
    9. regulation of blood volume by renin-angiotensin Source: MGI
    10. regulation of MAPK cascade Source: Ensembl
    11. renin-angiotensin regulation of aldosterone production Source: MGI
    12. response to cAMP Source: Ensembl
    13. response to cGMP Source: Ensembl
    14. response to lipopolysaccharide Source: Ensembl
    15. response to organic substance Source: MGI

    Keywords - Molecular functioni

    Aspartyl protease, Hydrolase, Protease

    Enzyme and pathway databases

    ReactomeiREACT_196539. Metabolism of Angiotensinogen to Angiotensins.

    Protein family/group databases

    MEROPSiA01.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Renin-1 (EC:3.4.23.15)
    Alternative name(s):
    Angiotensinogenase
    Kidney renin
    Gene namesi
    Name:Ren1
    Synonyms:Ren, Ren-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:97898. Ren1.

    Subcellular locationi

    Secreted By similarity. Membrane By similarity
    Note: Associated to membranes via binding to ATP6AP2.By similarity

    GO - Cellular componenti

    1. extracellular space Source: MGI
    2. intracellular Source: MGI
    3. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21211 PublicationAdd
    BLAST
    Propeptidei22 – 7150Activation peptide1 PublicationPRO_0000026089Add
    BLAST
    Chaini72 – 402331Renin-1PRO_0000026090Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi115 ↔ 122By similarity
    Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi278 ↔ 282By similarity
    Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP06281.
    PaxDbiP06281.
    PRIDEiP06281.

    PTM databases

    PhosphoSiteiP06281.

    Expressioni

    Tissue specificityi

    Kidney.

    Inductioni

    Renal renin is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration.

    Gene expression databases

    ArrayExpressiP06281.
    BgeeiP06281.
    CleanExiMM_REN1.
    GenevestigatoriP06281.

    Interactioni

    Subunit structurei

    Interacts with ATP6AP2.By similarity

    Protein-protein interaction databases

    IntActiP06281. 1 interaction.
    MINTiMINT-4132153.

    Structurei

    3D structure databases

    ProteinModelPortaliP06281.
    SMRiP06281. Positions 28-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase A1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG248684.
    GeneTreeiENSGT00710000106265.
    HOGENOMiHOG000197681.
    HOVERGENiHBG000482.
    InParanoidiP06281.
    KOiK01380.
    OMAiVVCEEGC.
    OrthoDBiEOG7HQN88.
    PhylomeDBiP06281.
    TreeFamiTF314990.

    Family and domain databases

    Gene3Di2.40.70.10. 2 hits.
    InterProiIPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR012848. Aspartic_peptidase_N.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view]
    PANTHERiPTHR13683. PTHR13683. 1 hit.
    PfamiPF07966. A1_Propeptide. 1 hit.
    PF00026. Asp. 1 hit.
    [Graphical view]
    PRINTSiPR00792. PEPSIN.
    SUPFAMiSSF50630. SSF50630. 1 hit.
    PROSITEiPS00141. ASP_PROTEASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06281-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRRRMPLWA LLLLWSPCTF SLPTRTATFE RIPLKKMPSV REILEERGVD    50
    MTRLSAEWGV FTKRPSLTNL TSPVVLTNYL NTQYYGEIGI GTPPQTFKVI 100
    FDTGSANLWV PSTKCSRLYL ACGIHSLYES SDSSSYMENG SDFTIHYGSG 150
    RVKGFLSQDS VTVGGITVTQ TFGEVTELPL IPFMLAKFDG VLGMGFPAQA 200
    VGGVTPVFDH ILSQGVLKEE VFSVYYNRGS HLLGGEVVLG GSDPQHYQGN 250
    FHYVSISKTD SWQITMKGVS VGSSTLLCEE GCAVVVDTGS SFISAPTSSL 300
    KLIMQALGAK EKRIEEYVVN CSQVPTLPDI SFDLGGRAYT LSSTDYVLQY 350
    PNRRDKLCTL ALHAMDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL 400
    AR 402
    Length:402
    Mass (Da):44,343
    Last modified:January 1, 1988 - v1
    Checksum:iD42920B555E97A38
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 2318Missing(PubMed:6370686)CuratedAdd
    BLAST
    Sequence conflicti24 – 241T → I(PubMed:6370686)Curated
    Sequence conflicti163 – 1631V → VSRV(PubMed:6370686)Curated

    Polymorphismi

    In inbred mouse strains, there are at least two alleles which can occur at the Ren1 locus: Ren-1D and Ren-1C. The sequence shown is that of Ren-1C.
    Present as a single-copy gene in strains such as BALB/c and C57BL/6 while some strains such as Swiss and Akr contain two copies.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti58 – 581W → R in allele Ren-1D.
    Natural varianti68 – 681T → I in allele Ren-1D.
    Natural varianti160 – 1601S → V in allele Ren-1D.
    Natural varianti315 – 3151E → D in allele Ren-1D. 1 Publication
    Natural varianti352 – 3521N → Y in allele Ren-1D.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00810
    , X00811, X00812, X00813, X00814, X00815, X00816, X00850, X00851 Genomic DNA. Translation: CAA25391.1.
    X16642 mRNA. Translation: CAA34636.1.
    K02596 Genomic DNA. Translation: AAA40045.1.
    M32352 Genomic DNA. Translation: AAA40043.1.
    AK052685 mRNA. Translation: BAC35094.1.
    AK085309 mRNA. Translation: BAC39418.1.
    BC061053 mRNA. Translation: AAH61053.1.
    K02800 Genomic DNA. Translation: AAA40044.1.
    M34190 Genomic DNA. Translation: AAA40042.1.
    CCDSiCCDS15295.1.
    PIRiA00989. REMSK.
    RefSeqiNP_112469.1. NM_031192.3.
    UniGeneiMm.220955.

    Genome annotation databases

    EnsembliENSMUST00000094556; ENSMUSP00000092135; ENSMUSG00000070645.
    GeneIDi19701.
    KEGGimmu:19701.
    UCSCiuc007cqf.1. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00810
    , X00811 , X00812 , X00813 , X00814 , X00815 , X00816 , X00850 , X00851 Genomic DNA. Translation: CAA25391.1 .
    X16642 mRNA. Translation: CAA34636.1 .
    K02596 Genomic DNA. Translation: AAA40045.1 .
    M32352 Genomic DNA. Translation: AAA40043.1 .
    AK052685 mRNA. Translation: BAC35094.1 .
    AK085309 mRNA. Translation: BAC39418.1 .
    BC061053 mRNA. Translation: AAH61053.1 .
    K02800 Genomic DNA. Translation: AAA40044.1 .
    M34190 Genomic DNA. Translation: AAA40042.1 .
    CCDSi CCDS15295.1.
    PIRi A00989. REMSK.
    RefSeqi NP_112469.1. NM_031192.3.
    UniGenei Mm.220955.

    3D structure databases

    ProteinModelPortali P06281.
    SMRi P06281. Positions 28-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P06281. 1 interaction.
    MINTi MINT-4132153.

    Chemistry

    BindingDBi P06281.
    ChEMBLi CHEMBL2615.

    Protein family/group databases

    MEROPSi A01.007.

    PTM databases

    PhosphoSitei P06281.

    Proteomic databases

    MaxQBi P06281.
    PaxDbi P06281.
    PRIDEi P06281.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000094556 ; ENSMUSP00000092135 ; ENSMUSG00000070645 .
    GeneIDi 19701.
    KEGGi mmu:19701.
    UCSCi uc007cqf.1. mouse.

    Organism-specific databases

    CTDi 19701.
    MGIi MGI:97898. Ren1.

    Phylogenomic databases

    eggNOGi NOG248684.
    GeneTreei ENSGT00710000106265.
    HOGENOMi HOG000197681.
    HOVERGENi HBG000482.
    InParanoidi P06281.
    KOi K01380.
    OMAi VVCEEGC.
    OrthoDBi EOG7HQN88.
    PhylomeDBi P06281.
    TreeFami TF314990.

    Enzyme and pathway databases

    Reactomei REACT_196539. Metabolism of Angiotensinogen to Angiotensins.

    Miscellaneous databases

    NextBioi 297064.
    PROi P06281.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P06281.
    Bgeei P06281.
    CleanExi MM_REN1.
    Genevestigatori P06281.

    Family and domain databases

    Gene3Di 2.40.70.10. 2 hits.
    InterProi IPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR012848. Aspartic_peptidase_N.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view ]
    PANTHERi PTHR13683. PTHR13683. 1 hit.
    Pfami PF07966. A1_Propeptide. 1 hit.
    PF00026. Asp. 1 hit.
    [Graphical view ]
    PRINTSi PR00792. PEPSIN.
    SUPFAMi SSF50630. SSF50630. 1 hit.
    PROSITEi PS00141. ASP_PROTEASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of aspartyl proteases by gene duplication: the mouse renin gene is organized in two homologous clusters of four exons."
      Holm I., Ollo R., Panthier J.-J., Rougeon F.
      EMBO J. 3:557-562(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
    2. "Nucleotide sequence of a cDNA coding for mouse Ren1 preprorenin."
      Kim W.S., Murakami K., Nakayama K.
      Nucleic Acids Res. 17:9480-9480(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Kidney.
    3. "The nucleotide sequence of a mouse renin-encoding gene, Ren-1d, and its upstream region."
      Burt D.W., Mullins L.J., George H., Smith G., Brooks J., Pioli D., Brammar W.J.
      Gene 84:91-104(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/10 and DBA/2.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    6. "Mouse kidney and submaxillary gland renin genes differ in their 5' putative regulatory sequences."
      Panthier J.-J., Dreyfus M., Roux D.T.L., Rougeon F.
      Proc. Natl. Acad. Sci. U.S.A. 81:5489-5493(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
      Tissue: Kidney.
    7. "Expression of tissue-specific Ren-1 and Ren-2 genes of mice: comparative analysis of 5'-proximal flanking regions."
      Field L.J., Philbrick W.M., Howles P.N., Dickinson D.P., McGowan R.A., Gross K.W.
      Mol. Cell. Biol. 4:2321-2331(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
    8. Cited for: PROTEIN SEQUENCE OF 22-37 AND 72-80.
      Strain: C57BL/10ROS X C3H/HEROS.
      Tissue: Kidney.
    9. "Molecular cloning of two distinct renin genes from the DBA/2 mouse."
      Mullins J.J., Burt D.W., Windass J.D., McTurk P., George H., Brammar W.J.
      EMBO J. 1:1461-1466(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 269-316, VARIANT ASP-315.
      Strain: DBA/2.
      Tissue: Submandibular gland.

    Entry informationi

    Entry nameiRENI1_MOUSE
    AccessioniPrimary (citable) accession number: P06281
    Secondary accession number(s): P97911
    , Q543E5, Q62153, Q62154
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 144 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3