Reviewed,
UniProtKB/Swiss-Prot P06281 (RENI1_MOUSE)
Last modified
June 16, 2009.
Version 101.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Renin-1 EC=3.4.23.15 Alternative name(s): Angiotensinogenase Kidney renin | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 402 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. |
| Catalytic activity | Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I. |
| Enzyme regulation | Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing By similarity. |
| Subunit structure | Interacts with ATP6AP2 By similarity. |
| Subcellular location | Secreted By similarity. Membrane By similarity. Note: Associated to membranes via binding to ATP6AP2 By similarity. |
| Tissue specificity | Kidney. |
| Induction | Renal renin is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. |
| Polymorphism | In inbred mouse strains, there are at least two alleles which can occur at the Ren1 locus: Ren-1D and Ren-1C. The sequence shown is that of Ren-1C. |
| Sequence similarities | Belongs to the peptidase A1 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane Secreted |
| Coding sequence diversity | Polymorphism |
| Domain | Signal |
| Molecular function | Aspartyl protease Hydrolase Protease |
| PTM | Disulfide bond Glycoprotein Zymogen |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | angiotensin maturation Inferred from mutant phenotype. Source: MGI cell maturationInferred from mutant phenotype. Source: MGI drinking behaviorInferred from mutant phenotype. Source: MGI hormone-mediated signalingInferred from direct assay. Source: MGI proteolysisInferred from electronic annotation. Source: InterPro regulation of blood volume by renin-angiotensinInferred from mutant phenotype. Source: MGI |
| Cellular component | extracellular space Inferred from direct assay. Source: MGI membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 21 | 21 | Ref.8 | ||||||||
| Propeptide | 22 – 71 | 50 | Activation peptide | PRO_0000026089 | |||||||
| Chain | 72 – 402 | 331 | Renin-1 | PRO_0000026090 | |||||||
Sites | |||||||||||
| Active site | 102 | 1 | By similarity | ||||||||
| Active site | 287 | 1 | By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 69 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 139 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 320 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 115 ↔ 122 | By similarity | |||||||||
| Disulfide bond | 278 ↔ 282 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 58 | 1 | W → R in allele Ren-1D. | ||||||||
| Natural variant | 68 | 1 | T → I in allele Ren-1D. | ||||||||
| Natural variant | 160 | 1 | S → V in allele Ren-1D. | ||||||||
| Natural variant | 315 | 1 | E → D in allele Ren-1D. Ref.9 | ||||||||
| Natural variant | 352 | 1 | N → Y in allele Ren-1D. | ||||||||
Experimental info | |||||||||||
| Sequence conflict | 6 – 23 | 18 | Missing Ref.1 | ||||||||
| Sequence conflict | 24 | 1 | T → I Ref.1 | ||||||||
| Sequence conflict | 163 | 1 | V → VSRV Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Evolution of aspartyl proteases by gene duplication: the mouse renin gene is organized in two homologous clusters of four exons." Holm I., Ollo R., Panthier J.-J., Rougeon F. EMBO J. 3:557-562(1984) [PubMed: 6370686] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. |
| [2] | "Nucleotide sequence of a cDNA coding for mouse Ren1 preprorenin." Kim W.S., Murakami K., Nakayama K. Nucleic Acids Res. 17:9480-9480(1989) [PubMed: 2685761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Kidney. |
| [3] | "The nucleotide sequence of a mouse renin-encoding gene, Ren-1d, and its upstream region." Burt D.W., Mullins L.J., George H., Smith G., Brooks J., Pioli D., Brammar W.J. Gene 84:91-104(1989) [PubMed: 2691339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: C57BL/10 and DBA/2. |
| [4] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
| [6] | "Mouse kidney and submaxillary gland renin genes differ in their 5' putative regulatory sequences." Panthier J.-J., Dreyfus M., Roux D.T.L., Rougeon F. Proc. Natl. Acad. Sci. U.S.A. 81:5489-5493(1984) [PubMed: 6089205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30. Tissue: Kidney. |
| [7] | "Expression of tissue-specific Ren-1 and Ren-2 genes of mice: comparative analysis of 5'-proximal flanking regions." Field L.J., Philbrick W.M., Howles P.N., Dickinson D.P., McGowan R.A., Gross K.W. Mol. Cell. Biol. 4:2321-2331(1984) [PubMed: 6392850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31. |
| [8] | "Biosynthesis of renin in mouse kidney tumor As4.1 cells." Jones C.A., Petrovic N., Novak E.K., Swank R.T., Sigmund C.D., Gross K.W. Eur. J. Biochem. 243:181-190(1997) [PubMed: 9030738] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-37 AND 72-80. Strain: C57BL/10ROS X C3H/HEROS. Tissue: Kidney. |
| [9] | "Molecular cloning of two distinct renin genes from the DBA/2 mouse." Mullins J.J., Burt D.W., Windass J.D., McTurk P., George H., Brammar W.J. EMBO J. 1:1461-1466(1982) [PubMed: 6327270] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 269-316, VARIANT ASP-315. Strain: DBA/2. Tissue: Submandibular gland. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
X00810 X00851 Genomic DNA. Translation: CAA25391.1. X16642 mRNA. Translation: CAA34636.1. K02596 Genomic DNA. Translation: AAA40045.1. M32352 Genomic DNA. Translation: AAA40043.1. AK052685 mRNA. Translation: BAC35094.1. AK085309 mRNA. Translation: BAC39418.1. BC061053 mRNA. Translation: AAH61053.1. K02800 Genomic DNA. Translation: AAA40044.1. M34190 Genomic DNA. Translation: AAA40042.1. | |
| IPI | IPI00121828. |
| PIR | REMSK. A00989. |
| RefSeq | NP_112469.1. |
| UniGene | Mm.220955 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1SMR based on UniProtKB P00796. |
| SMR | P06281. Positions 69-402. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | A01.007. |
Proteomic databases | |
| PRIDE | P06281. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000070645. Mus musculus. [Contig view] |
| GeneID | 19701. |
| KEGG | mmu:19701. |
Organism-specific databases | |
| MGI | MGI:97898. Ren1. |
Phylogenomic databases | |
| HOGENOM | P06281. |
| HOVERGEN | P06281. |
Enzyme and pathway databases | |
| BRENDA | 3.4.23.15. 244. |
Gene expression databases | |
| ArrayExpress | P06281. |
| Bgee | P06281. |
| CleanEx | MM_REN1. |
| GermOnline | ENSMUSG00000070645. Mus musculus. |
Family and domain databases | |
| InterPro | IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view] |
| Gene3D | G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits. |
| PANTHER | PTHR13683. Peptidase_A1. 1 hit. |
| Pfam | PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view] |
| PRINTS | PR00792. PEPSIN. |
| PROSITE | PS00141. ASP_PROTEASE. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 297064. |
| SOURCE | Search... |
Entry information
| Entry name | RENI1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P06281 Secondary accession number(s): P97911 Q62154 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


