P06245 (KAPB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cAMP-dependent protein kinase type 2
Short name=PKA 2
EC=2.7.11.11

Gene names

Name:	TPK2
Synonyms:	PKA2, YKR1
Ordered Locus Names:	YPL203W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by cAMP.
Miscellaneous	Present with 2220 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding cAMP
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	invasive growth in response to glucose limitation Inferred from mutant phenotype. Source: SGD
Cellular component	cAMP-dependent protein kinase complex Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cAMP-dependent protein kinase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCY1	P07278	10	EBI-9465,EBI-9475

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	cAMP-dependent protein kinase type 2		PRO_0000086048
Regions
	Domain	70 – 324	255	Protein kinase
	Domain	325 – 380	56	AGC-kinase C-terminal
	Nucleotide binding	76 – 84	9	ATP By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	99	1	ATP By similarity
Amino acid modifications
	Modified residue	224	1	Phosphothreonine Ref.6 Ref.7
Experimental info
	Sequence conflict	214 – 220	7	FAKEVQT → LRQRGTN in CAA68689. Ref.2
	Sequence conflict	260	1	A → V in CAA68689. Ref.2
	Sequence conflict	289	1	H → Q in AAA35165. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P06245 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 1BC255C6D0F33137	FASTA	380	44,220
	10 20 30 40 50 60 MEFVAERAQP VGQTIQQQNV NTYGQGVLQP HHDLQQRQQQ QQQRQHQQLL TSQLPQKSLV 70 80 90 100 110 120 SKGKYTLHDF QIMRTLGTGS FGRVHLVRSV HNGRYYAIKV LKKQQVVKMK QVEHTNDERR 130 140 150 160 170 180 MLKLVEHPFL IRMWGTFQDA RNIFMVMDYI EGGELFSLLR KSQRFPNPVA KFYAAEVILA 190 200 210 220 230 240 LEYLHAHNII YRDLKPENIL LDRNGHIKIT DFGFAKEVQT VTWTLCGTPD YIAPEVITTK 250 260 270 280 290 300 PYNKSVDWWS LGVLIYEMLA GYTPFYDTTP MKTYEKILQG KVVYPPYFHP DVVDLLSKLI 310 320 330 340 350 360 TADLTRRIGN LQSGSRDIKA HPWFSEVVWE RLLAKDIETP YEPPITSGIG DTSLFDQYPE 370 380 EQLDYGIQGD DPYAEYFQDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase." Toda T., Cameron S., Sass P., Zoller M., Wigler M. Cell 50:277-287(1987) [PubMed: 3036373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A yeast gene coding for a putative protein kinase homologous to cdc25 suppressing protein kinase." Ohno S., Aoshima M., Matsumoto S., Yahara I., Suzuki K. FEBS Lett. 222:279-285(1987) [PubMed: 3308514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26786 / X2180-1A.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-224, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-224, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M17073 Genomic DNA. Translation: AAA35165.1. Y00694 Genomic DNA. Translation: CAA68689.1. Z73559 Genomic DNA. Translation: CAA97917.1. BK006949 Genomic DNA. Translation: DAA11232.1.
PIR	OKBYC2. S65222.
RefSeq	NP_015121.1. NM_001184017.1.
3D structure databases
ProteinModelPortal	P06245.
SMR	P06245. Positions 65-380.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-549N.
IntAct	P06245. 40 interactions.
MINT	MINT-504980.
STRING	P06245.
Proteomic databases
PeptideAtlas	P06245.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL203W; YPL203W; YPL203W.
GeneID	855898.
KEGG	sce:YPL203W.
NMPDR	fig|4932.3.peg.6249.
Organism-specific databases
CYGD	YPL203w.
SGD	S000006124. TPK2.
Phylogenomic databases
eggNOG	fuNOG04187.
GeneTree	EFGT00070000008712.
HOGENOM	HBG755340.
OMA	VTWTLCG.
OrthoDB	EOG4JDMGC.
Enzyme and pathway databases
BRENDA	2.7.11.11. 984.
Gene expression databases
ArrayExpress	P06245.
Genevestigator	P06245.
GermOnline	YPL203W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K04345.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980583.

Entry information

Entry name

KAPB_YEAST

Accession

Primary (citable) accession number: P06245
Secondary accession number(s): D6W3G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program