cAMP-dependent protein kinase type 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P06244 (KAPA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 137. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
cAMP-dependent protein kinase type 1
Short name=PKA 1
EC=2.7.11.11

Alternative name(s):
CDC25-suppressing protein kinase
PK-25

Gene names

Name:	TPK1
Synonyms:	PKA1, SRA3
Ordered Locus Names:	YJL164C
ORF Names:	J0541

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by cAMP.
Subcellular location	Cytoplasm. Nucleus Ref.7.
Miscellaneous	Present with 4320 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.
Sequence caution	The sequence AAA35088.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding cAMP
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Ras protein signal transduction Inferred from mutant phenotype Ref.3. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cAMP-dependent protein kinase complex Inferred from direct assay. Source: SGD cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cAMP-dependent protein kinase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCY1	P07278	7	EBI-9458,EBI-9475

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 397

397

cAMP-dependent protein kinase type 1

PRO_0000086046

Regions

Domain

87 – 341

255

Protein kinase

Domain

342 – 397

AGC-kinase C-terminal

Nucleotide binding

93 – 101

ATP By similarity

Sites

Active site

210

Proton acceptor

Binding site

116

ATP By similarity

Amino acid modifications

Modified residue

239

Phosphothreonine Ref.11

Modified residue

240

Phosphotyrosine Ref.11

Modified residue

241

Phosphothreonine Ref.9 Ref.10 Ref.11

Experimental info

Sequence conflict

79 – 86

SGKYSLQD → VGSIVYKN in AAA35088. Ref.3

Sequence conflict

79 – 86

SGKYSLQD → VGSIVYKN in CAA89459. Ref.4

Sequence conflict

164 – 171

MDYIEGGE → ILKVER in AAA35088. Ref.3

Sequence conflict

180 – 181

QR → KD in AAA35088. Ref.3

Sequence conflict

188 – 190

KFY → QIF in AAA35088. Ref.3

Secondary structure

397

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P06244 [UniParc].

Last modified September 21, 2011. Version 3.
Checksum: D4E5C30D7F11458B
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FASTA

397

46,076

        10         20         30         40         50         60 
MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE TQEKPKQPHV 

        70         80         90        100        110        120 
TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR VHLIRSRHNG RYYAMKVLKK 

       130        140        150        160        170        180 
EIVVRLKQVE HTNDERLMLS IVTHPFIIRM WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ 

       190        200        210        220        230        240 
RFPNPVAKFY AAEVCLALEY LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY 

       250        260        270        280        290        300 
TLCGTPDYIA PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR 

       310        320        330        340        350        360 
FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL SRNIETPYEP 

       370        380        390 
PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase." Toda T., Cameron S., Sass P., Zoller M., Wigler M. Cell 50:277-287(1987) [PubMed: 3036373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation and nucleotide sequence of a Saccharomyces cerevisiae protein kinase gene suppressing the cell cycle start mutation cdc25." Lisziewicz J., Godany A., Foerster H.-H., Kuentzel H. J. Biol. Chem. 262:2549-2553(1987) [PubMed: 3546292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase." Cannon J.F., Tatchell K. Mol. Cell. Biol. 7:2653-2663(1987) [PubMed: 2823100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 79-86. Strain: ATCC 204508 / S288c.
[6]	"Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE." Zhang Z., Dietrich F.S. Nucleic Acids Res. 33:2838-2851(2005) [PubMed: 15905473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-82. Strain: ATCC 208353 / W303-1A.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-241, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-241, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-239; TYR-240 AND THR-241, MASS SPECTROMETRY.
[12]	"Structure of the unliganded cAMP-dependent protein kinase catalytic subunit from Saccharomyces cerevisiae." Mashhoon N., Carmel G., Pflugrath J.W., Kuret J. Arch. Biochem. Biophys. 387:11-19(2001) [PubMed: 11368172] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 81-397.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17072 Genomic DNA. Translation: AAA35164.1.
J02665 Genomic DNA. Translation: AAA34877.1.
M17224 Genomic DNA. Translation: AAA35088.1. Different initiation.
Z49439 Genomic DNA. Translation: CAA89459.1.
AY899257 mRNA. Translation: AAX83942.1.
BK006943 Genomic DNA. Translation: DAA08639.2.

PIR

OKBYC1. JC1034.

RefSeq

NP_012371.2. NM_001181597.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FOT	X-ray	2.80	A	81-397	[»]

ProteinModelPortal

P06244.

SMR

P06244. Positions 82-397.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-548N.

IntAct

P06244. 23 interactions.

MINT

MINT-569684.

STRING

P06244.

Proteomic databases

PeptideAtlas

P06244.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

853275.

KEGG

sce:YJL164C.

Organism-specific databases

SGD

S000003700. TPK1.

Phylogenomic databases

eggNOG

fuNOG09673.

GeneTree

EFGT00070000008712.

HOGENOM

HBG755340.

OMA

NAATANK.

OrthoDB

EOG4JDMGC.

Enzyme and pathway databases

BRENDA

2.7.11.11. 984.

Gene expression databases

ArrayExpress

P06244.

Genevestigator

P06244.

GermOnline

YJL164C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_kinase-like_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR002290. Ser/Thr_kinase_dom.
[Graphical view]

K04345.

Pfam

PF00069. Pkinase. 1 hit.
[Graphical view]

SMART

SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KAPA_YEAST

Accession

Primary (citable) accession number: P06244
Secondary accession number(s): D6VW23, P11595, Q2VQW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	September 21, 2011
Last modified:	January 25, 2012
This is version 137 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents