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Protein

cAMP-dependent protein kinase type 1

Gene

TPK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by cAMP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116ATPPROSITE-ProRule annotation1
Active sitei210Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi93 – 101ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cAMP-dependent protein kinase activity Source: SGD

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • protein kinase A signaling Source: SGD
  • protein phosphorylation Source: SGD
  • Ras protein signal transduction Source: SGD
  • regulation of macroautophagy Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

ATP-binding, cAMP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31604-MONOMER.
BRENDAi2.7.11.11. 984.
ReactomeiR-SCE-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-SCE-163615. PKA activation.
R-SCE-164378. PKA activation in glucagon signalling.
R-SCE-180024. DARPP-32 events.
R-SCE-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-SCE-5610787. Hedgehog 'off' state.
R-SCE-5687128. MAPK6/MAPK4 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type 1 (EC:2.7.11.11)
Short name:
PKA 1
Alternative name(s):
CDC25-suppressing protein kinase
PK-25
Gene namesi
Name:TPK1
Synonyms:PKA1, SRA3
Ordered Locus Names:YJL164C
ORF Names:J0541
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL164C.
SGDiS000003700. TPK1.

Subcellular locationi

GO - Cellular componenti

  • cAMP-dependent protein kinase complex Source: SGD
  • cytoplasm Source: SGD
  • nuclear chromatin Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000860462 – 397cAMP-dependent protein kinase type 1Add BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP06244.
PRIDEiP06244.

PTM databases

iPTMnetiP06244.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BCY1P072785EBI-9458,EBI-9475

Protein-protein interaction databases

BioGridi33595. 378 interactors.
DIPiDIP-548N.
IntActiP06244. 15 interactors.
MINTiMINT-569684.

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi84 – 86Combined sources3
Beta strandi87 – 95Combined sources9
Beta strandi100 – 106Combined sources7
Turni107 – 109Combined sources3
Beta strandi112 – 119Combined sources8
Helixi120 – 125Combined sources6
Helixi129 – 140Combined sources12
Beta strandi150 – 155Combined sources6
Beta strandi157 – 164Combined sources8
Helixi172 – 178Combined sources7
Helixi184 – 202Combined sources19
Turni203 – 205Combined sources3
Helixi213 – 215Combined sources3
Beta strandi216 – 218Combined sources3
Beta strandi224 – 226Combined sources3
Turni246 – 248Combined sources3
Helixi251 – 254Combined sources4
Helixi263 – 277Combined sources15
Helixi287 – 296Combined sources10
Helixi307 – 316Combined sources10
Turni321 – 323Combined sources3
Turni329 – 332Combined sources4
Helixi333 – 336Combined sources4
Helixi339 – 341Combined sources3
Helixi346 – 350Combined sources5
Helixi391 – 393Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FOTX-ray2.80A81-397[»]
ProteinModelPortaliP06244.
SMRiP06244.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06244.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 341Protein kinasePROSITE-ProRule annotationAdd BLAST255
Domaini342 – 397AGC-kinase C-terminalAdd BLAST56

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00810000125385.
HOGENOMiHOG000233033.
InParanoidiP06244.
KOiK04345.
OMAiAKKDTEQ.
OrthoDBiEOG092C0HH3.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE
60 70 80 90 100
TQEKPKQPHV TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR
110 120 130 140 150
VHLIRSRHNG RYYAMKVLKK EIVVRLKQVE HTNDERLMLS IVTHPFIIRM
160 170 180 190 200
WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ RFPNPVAKFY AAEVCLALEY
210 220 230 240 250
LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY TLCGTPDYIA
260 270 280 290 300
PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR
310 320 330 340 350
FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL
360 370 380 390
SRNIETPYEP PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF
Length:397
Mass (Da):46,076
Last modified:September 21, 2011 - v3
Checksum:iD4E5C30D7F11458B
GO

Sequence cautioni

The sequence AAA35088 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79 – 86SGKYSLQD → VGSIVYKN in AAA35088 (PubMed:2823100).Curated8
Sequence conflicti79 – 86SGKYSLQD → VGSIVYKN in CAA89459 (PubMed:8641269).Curated8
Sequence conflicti164 – 171MDYIEGGE → ILKVER in AAA35088 (PubMed:2823100).Curated8
Sequence conflicti180 – 181QR → KD in AAA35088 (PubMed:2823100).Curated2
Sequence conflicti188 – 190KFY → QIF in AAA35088 (PubMed:2823100).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17072 Genomic DNA. Translation: AAA35164.1.
J02665 Genomic DNA. Translation: AAA34877.1.
M17224 Genomic DNA. Translation: AAA35088.1. Different initiation.
Z49439 Genomic DNA. Translation: CAA89459.1.
AY899257 mRNA. Translation: AAX83942.1.
BK006943 Genomic DNA. Translation: DAA08639.2.
PIRiJC1034. OKBYC1.
RefSeqiNP_012371.2. NM_001181597.2.

Genome annotation databases

EnsemblFungiiYJL164C; YJL164C; YJL164C.
GeneIDi853275.
KEGGisce:YJL164C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17072 Genomic DNA. Translation: AAA35164.1.
J02665 Genomic DNA. Translation: AAA34877.1.
M17224 Genomic DNA. Translation: AAA35088.1. Different initiation.
Z49439 Genomic DNA. Translation: CAA89459.1.
AY899257 mRNA. Translation: AAX83942.1.
BK006943 Genomic DNA. Translation: DAA08639.2.
PIRiJC1034. OKBYC1.
RefSeqiNP_012371.2. NM_001181597.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FOTX-ray2.80A81-397[»]
ProteinModelPortaliP06244.
SMRiP06244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33595. 378 interactors.
DIPiDIP-548N.
IntActiP06244. 15 interactors.
MINTiMINT-569684.

PTM databases

iPTMnetiP06244.

Proteomic databases

MaxQBiP06244.
PRIDEiP06244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL164C; YJL164C; YJL164C.
GeneIDi853275.
KEGGisce:YJL164C.

Organism-specific databases

EuPathDBiFungiDB:YJL164C.
SGDiS000003700. TPK1.

Phylogenomic databases

GeneTreeiENSGT00810000125385.
HOGENOMiHOG000233033.
InParanoidiP06244.
KOiK04345.
OMAiAKKDTEQ.
OrthoDBiEOG092C0HH3.

Enzyme and pathway databases

BioCyciYEAST:G3O-31604-MONOMER.
BRENDAi2.7.11.11. 984.
ReactomeiR-SCE-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-SCE-163615. PKA activation.
R-SCE-164378. PKA activation in glucagon signalling.
R-SCE-180024. DARPP-32 events.
R-SCE-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-SCE-5610787. Hedgehog 'off' state.
R-SCE-5687128. MAPK6/MAPK4 signaling.

Miscellaneous databases

EvolutionaryTraceiP06244.
PROiP06244.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAPA_YEAST
AccessioniPrimary (citable) accession number: P06244
Secondary accession number(s): D6VW23, P11595, Q2VQW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: September 21, 2011
Last modified: November 2, 2016
This is version 181 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4320 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.