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P06244

- KAPA_YEAST

UniProt

P06244 - KAPA_YEAST

Protein

cAMP-dependent protein kinase type 1

Gene

TPK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 160 (01 Oct 2014)
      Sequence version 3 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by cAMP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161ATPPROSITE-ProRule annotation
    Active sitei210 – 2101Proton acceptor

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi93 – 1019ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cAMP-dependent protein kinase activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. protein kinase A signaling Source: SGD
    4. protein phosphorylation Source: SGD
    5. Ras protein signal transduction Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    ATP-binding, cAMP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31604-MONOMER.
    BRENDAi2.7.11.11. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-dependent protein kinase type 1 (EC:2.7.11.11)
    Short name:
    PKA 1
    Alternative name(s):
    CDC25-suppressing protein kinase
    PK-25
    Gene namesi
    Name:TPK1
    Synonyms:PKA1, SRA3
    Ordered Locus Names:YJL164C
    ORF Names:J0541
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    SGDiS000003700. TPK1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cAMP-dependent protein kinase complex Source: SGD
    2. cytoplasm Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 397396cAMP-dependent protein kinase type 1PRO_0000086046Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP06244.
    PaxDbiP06244.
    PeptideAtlasiP06244.

    Expressioni

    Gene expression databases

    GenevestigatoriP06244.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BCY1P072785EBI-9458,EBI-9475

    Protein-protein interaction databases

    BioGridi33595. 378 interactions.
    DIPiDIP-548N.
    IntActiP06244. 15 interactions.
    MINTiMINT-569684.

    Structurei

    Secondary structure

    1
    397
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi84 – 863
    Beta strandi87 – 959
    Beta strandi100 – 1067
    Turni107 – 1093
    Beta strandi112 – 1198
    Helixi120 – 1256
    Helixi129 – 14012
    Beta strandi150 – 1556
    Beta strandi157 – 1648
    Helixi172 – 1787
    Helixi184 – 20219
    Turni203 – 2053
    Helixi213 – 2153
    Beta strandi216 – 2183
    Beta strandi224 – 2263
    Turni246 – 2483
    Helixi251 – 2544
    Helixi263 – 27715
    Helixi287 – 29610
    Helixi307 – 31610
    Turni321 – 3233
    Turni329 – 3324
    Helixi333 – 3364
    Helixi339 – 3413
    Helixi346 – 3505
    Helixi391 – 3933

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FOTX-ray2.80A81-397[»]
    ProteinModelPortaliP06244.
    SMRiP06244. Positions 51-397.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP06244.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini87 – 341255Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini342 – 39756AGC-kinase C-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074358.
    HOGENOMiHOG000233033.
    KOiK04345.
    OMAiFHEPDIT.
    OrthoDBiEOG7R2BT5.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06244-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE    50
    TQEKPKQPHV TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR 100
    VHLIRSRHNG RYYAMKVLKK EIVVRLKQVE HTNDERLMLS IVTHPFIIRM 150
    WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ RFPNPVAKFY AAEVCLALEY 200
    LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY TLCGTPDYIA 250
    PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR 300
    FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL 350
    SRNIETPYEP PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF 397
    Length:397
    Mass (Da):46,076
    Last modified:September 21, 2011 - v3
    Checksum:iD4E5C30D7F11458B
    GO

    Sequence cautioni

    The sequence AAA35088.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti79 – 868SGKYSLQD → VGSIVYKN in AAA35088. (PubMed:2823100)Curated
    Sequence conflicti79 – 868SGKYSLQD → VGSIVYKN in CAA89459. (PubMed:8641269)Curated
    Sequence conflicti164 – 1718MDYIEGGE → ILKVER in AAA35088. (PubMed:2823100)Curated
    Sequence conflicti180 – 1812QR → KD in AAA35088. (PubMed:2823100)Curated
    Sequence conflicti188 – 1903KFY → QIF in AAA35088. (PubMed:2823100)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17072 Genomic DNA. Translation: AAA35164.1.
    J02665 Genomic DNA. Translation: AAA34877.1.
    M17224 Genomic DNA. Translation: AAA35088.1. Different initiation.
    Z49439 Genomic DNA. Translation: CAA89459.1.
    AY899257 mRNA. Translation: AAX83942.1.
    BK006943 Genomic DNA. Translation: DAA08639.2.
    PIRiJC1034. OKBYC1.
    RefSeqiNP_012371.2. NM_001181597.2.

    Genome annotation databases

    EnsemblFungiiYJL164C; YJL164C; YJL164C.
    GeneIDi853275.
    KEGGisce:YJL164C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17072 Genomic DNA. Translation: AAA35164.1 .
    J02665 Genomic DNA. Translation: AAA34877.1 .
    M17224 Genomic DNA. Translation: AAA35088.1 . Different initiation.
    Z49439 Genomic DNA. Translation: CAA89459.1 .
    AY899257 mRNA. Translation: AAX83942.1 .
    BK006943 Genomic DNA. Translation: DAA08639.2 .
    PIRi JC1034. OKBYC1.
    RefSeqi NP_012371.2. NM_001181597.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FOT X-ray 2.80 A 81-397 [» ]
    ProteinModelPortali P06244.
    SMRi P06244. Positions 51-397.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33595. 378 interactions.
    DIPi DIP-548N.
    IntActi P06244. 15 interactions.
    MINTi MINT-569684.

    Proteomic databases

    MaxQBi P06244.
    PaxDbi P06244.
    PeptideAtlasi P06244.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL164C ; YJL164C ; YJL164C .
    GeneIDi 853275.
    KEGGi sce:YJL164C.

    Organism-specific databases

    SGDi S000003700. TPK1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074358.
    HOGENOMi HOG000233033.
    KOi K04345.
    OMAi FHEPDIT.
    OrthoDBi EOG7R2BT5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31604-MONOMER.
    BRENDAi 2.7.11.11. 984.

    Miscellaneous databases

    EvolutionaryTracei P06244.
    NextBioi 973556.
    PROi P06244.

    Gene expression databases

    Genevestigatori P06244.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase."
      Toda T., Cameron S., Sass P., Zoller M., Wigler M.
      Cell 50:277-287(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Isolation and nucleotide sequence of a Saccharomyces cerevisiae protein kinase gene suppressing the cell cycle start mutation cdc25."
      Lisziewicz J., Godany A., Foerster H.-H., Kuentzel H.
      J. Biol. Chem. 262:2549-2553(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase."
      Cannon J.F., Tatchell K.
      Mol. Cell. Biol. 7:2653-2663(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 79-86.
      Strain: ATCC 204508 / S288c.
    6. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
      Zhang Z., Dietrich F.S.
      Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-82.
      Strain: ATCC 208353 / W303-1A.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    10. "Structure of the unliganded cAMP-dependent protein kinase catalytic subunit from Saccharomyces cerevisiae."
      Mashhoon N., Carmel G., Pflugrath J.W., Kuret J.
      Arch. Biochem. Biophys. 387:11-19(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 81-397.

    Entry informationi

    Entry nameiKAPA_YEAST
    AccessioniPrimary (citable) accession number: P06244
    Secondary accession number(s): D6VW23, P11595, Q2VQW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 160 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4320 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3