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Reviewed, UniProtKB/Swiss-Prot P06244 (KAPA_YEAST)

Last modified October 13, 2009. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    cAMP-dependent protein kinase type 1
      Short name=PKA 1
    EC=2.7.11.11
Alternative name(s):
    CDC25-suppressing protein kinase
    PK-25
Gene names
Name: TPK1
Synonyms: PKA1, SRA3
Ordered Locus Names: YJL164C
ORF Names: J0541
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by cAMP.

Miscellaneous

Present with 4320 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 397397cAMP-dependent protein kinase type 1
PRO_0000086046

Regions

Domain87 – 341255Protein kinase
Domain342 – 39756AGC-kinase C-terminal
Nucleotide binding93 – 1019ATP By similarity

Sites

Active site2101Proton acceptor
Binding site1161ATP By similarity

Amino acid modifications

Modified residue2391Phosphothreonine Ref.9
Modified residue2401Phosphotyrosine Ref.9
Modified residue2411Phosphothreonine Ref.9 Ref.7 Ref.8

Experimental info

Sequence conflict79 – 868SGKYSLQD → VGSIVYKN in AAA35088. Ref.3
Sequence conflict79 – 868SGKYSLQD → VGSIVYKN in CAA89459. Ref.4
Sequence conflict79 – 824SGKY → VGSI in AAX83942. Ref.5
Sequence conflict164 – 1718MDYIEGGE → ILKVER in AAA35088. Ref.3
Sequence conflict180 – 1812QR → KD in AAA35088. Ref.3
Sequence conflict188 – 1903KFY → QIF in AAA35088. Ref.3

Secondary structure

............................................... 397
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06244-1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: D4E5C30D7F11458B

FASTA39746,076
        10         20         30         40         50         60 
MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE TQEKPKQPHV 

        70         80         90        100        110        120 
TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR VHLIRSRHNG RYYAMKVLKK 

       130        140        150        160        170        180 
EIVVRLKQVE HTNDERLMLS IVTHPFIIRM WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ 

       190        200        210        220        230        240 
RFPNPVAKFY AAEVCLALEY LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY 

       250        260        270        280        290        300 
TLCGTPDYIA PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR 

       310        320        330        340        350        360 
FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL SRNIETPYEP 

       370        380        390 
PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF 

« Hide

References

« Hide 'large scale' references
[1]"Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase."
Toda T., Cameron S., Sass P., Zoller M., Wigler M.
Cell 50:277-287(1987) [PubMed: 3036373] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Isolation and nucleotide sequence of a Saccharomyces cerevisiae protein kinase gene suppressing the cell cycle start mutation cdc25."
Lisziewicz J., Godany A., Foerster H.-H., Kuentzel H.
J. Biol. Chem. 262:2549-2553(1987) [PubMed: 3546292] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase."
Cannon J.F., Tatchell K.
Mol. Cell. Biol. 7:2653-2663(1987) [PubMed: 2823100] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
Zhang Z., Dietrich F.S.
Nucleic Acids Res. 33:2838-2851(2005) [PubMed: 15905473] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-82.
Strain: ATCC 208353 / W303-1A.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-241, MASS SPECTROMETRY.
[8]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-241, MASS SPECTROMETRY.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-239; TYR-240 AND THR-241, MASS SPECTROMETRY.
[10]"Structure of the unliganded cAMP-dependent protein kinase catalytic subunit from Saccharomyces cerevisiae."
Mashhoon N., Carmel G., Pflugrath J.W., Kuret J.
Arch. Biochem. Biophys. 387:11-19(2001) [PubMed: 11368172] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 81-397.
+Additional computationally mapped references.

Cross-references

Sequence databases

J02665 Genomic DNA. Translation: AAA34877.1.
M17072 Genomic DNA. Translation: AAA35164.1.
M17224 Genomic DNA. Translation: AAA35088.1. Different initiation.
Z49439 Genomic DNA. Translation: CAA89459.1.
AY899257 mRNA. Translation: AAX83942.1.
PIROKBYC1. JC1034.
RefSeqNP_012371.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1FOTX-ray2.80A81-397[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:548N.
IntActP06244. 11 interactions.
STRINGP06244.

Proteomic databases

PeptideAtlasP06244.
PRIDEP06244.

Genome annotation databases

EnsemblYJL164C; YJL164C; YJL164C; Saccharomyces cerevisiae. [Genome view]
GeneID853275.
GenomeReviewsGene locus YJL164C in contig Y13136_GR.
KEGGsce:YJL164C.

Organism-specific databases

CYGDYJL164c.
SGDS000003700. TPK1.

Phylogenomic databases

HOGENOMP06244.

Enzyme and pathway databases

BRENDA2.7.11.11. 250.

Gene expression databases

ArrayExpressP06244.
GenevestigatorP06244.
GermOnlineYJL164C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAPA_YEAST
AccessionPrimary (citable) accession number: P06244
Secondary accession number(s): P11595, Q2VQW1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 13, 2009
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents