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P06244 (KAPA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 157. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cAMP-dependent protein kinase type 1

Short name=PKA 1
EC=2.7.11.11
Alternative name(s):
CDC25-suppressing protein kinase
PK-25
Gene names
Name:TPK1
Synonyms:PKA1, SRA3
Ordered Locus Names:YJL164C
ORF Names:J0541
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by cAMP.

Subcellular location

Cytoplasm. Nucleus Ref.7.

Miscellaneous

Present with 4320 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAA35088.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BCY1P072785EBI-9458,EBI-9475

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 397396cAMP-dependent protein kinase type 1
PRO_0000086046

Regions

Domain87 – 341255Protein kinase
Domain342 – 39756AGC-kinase C-terminal
Nucleotide binding93 – 1019ATP By similarity

Sites

Active site2101Proton acceptor
Binding site1161ATP By similarity

Amino acid modifications

Modified residue21N-acetylserine Ref.9

Experimental info

Sequence conflict79 – 868SGKYSLQD → VGSIVYKN in AAA35088. Ref.3
Sequence conflict79 – 868SGKYSLQD → VGSIVYKN in CAA89459. Ref.4
Sequence conflict164 – 1718MDYIEGGE → ILKVER in AAA35088. Ref.3
Sequence conflict180 – 1812QR → KD in AAA35088. Ref.3
Sequence conflict188 – 1903KFY → QIF in AAA35088. Ref.3

Secondary structure

............................................... 397
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06244 [UniParc].

Last modified September 21, 2011. Version 3.
Checksum: D4E5C30D7F11458B

FASTA39746,076
        10         20         30         40         50         60 
MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE TQEKPKQPHV 

        70         80         90        100        110        120 
TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR VHLIRSRHNG RYYAMKVLKK 

       130        140        150        160        170        180 
EIVVRLKQVE HTNDERLMLS IVTHPFIIRM WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ 

       190        200        210        220        230        240 
RFPNPVAKFY AAEVCLALEY LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY 

       250        260        270        280        290        300 
TLCGTPDYIA PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR 

       310        320        330        340        350        360 
FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL SRNIETPYEP 

       370        380        390 
PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF 

« Hide

References

« Hide 'large scale' references
[1]"Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase."
Toda T., Cameron S., Sass P., Zoller M., Wigler M.
Cell 50:277-287(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Isolation and nucleotide sequence of a Saccharomyces cerevisiae protein kinase gene suppressing the cell cycle start mutation cdc25."
Lisziewicz J., Godany A., Foerster H.-H., Kuentzel H.
J. Biol. Chem. 262:2549-2553(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase."
Cannon J.F., Tatchell K.
Mol. Cell. Biol. 7:2653-2663(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 79-86.
Strain: ATCC 204508 / S288c.
[6]"Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
Zhang Z., Dietrich F.S.
Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-82.
Strain: ATCC 208353 / W303-1A.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[10]"Structure of the unliganded cAMP-dependent protein kinase catalytic subunit from Saccharomyces cerevisiae."
Mashhoon N., Carmel G., Pflugrath J.W., Kuret J.
Arch. Biochem. Biophys. 387:11-19(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 81-397.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M17072 Genomic DNA. Translation: AAA35164.1.
J02665 Genomic DNA. Translation: AAA34877.1.
M17224 Genomic DNA. Translation: AAA35088.1. Different initiation.
Z49439 Genomic DNA. Translation: CAA89459.1.
AY899257 mRNA. Translation: AAX83942.1.
BK006943 Genomic DNA. Translation: DAA08639.2.
PIROKBYC1. JC1034.
RefSeqNP_012371.2. NM_001181597.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FOTX-ray2.80A81-397[»]
ProteinModelPortalP06244.
SMRP06244. Positions 51-397.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33595. 378 interactions.
DIPDIP-548N.
IntActP06244. 15 interactions.
MINTMINT-569684.

Proteomic databases

PaxDbP06244.
PeptideAtlasP06244.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL164C; YJL164C; YJL164C.
GeneID853275.
KEGGsce:YJL164C.

Organism-specific databases

SGDS000003700. TPK1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074358.
HOGENOMHOG000233033.
KOK04345.
OMAHISNTEK.
OrthoDBEOG7R2BT5.

Enzyme and pathway databases

BioCycYEAST:G3O-31604-MONOMER.
BRENDA2.7.11.11. 984.

Gene expression databases

GenevestigatorP06244.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP06244.
NextBio973556.
PROP06244.

Entry information

Entry nameKAPA_YEAST
AccessionPrimary (citable) accession number: P06244
Secondary accession number(s): D6VW23, P11595, Q2VQW1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: September 21, 2011
Last modified: April 16, 2014
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references