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P06244

- KAPA_YEAST

UniProt

P06244 - KAPA_YEAST

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Protein
cAMP-dependent protein kinase type 1
Gene
TPK1, PKA1, SRA3, YJL164C, J0541
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by cAMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161ATP By similarity
Active sitei210 – 2101Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi93 – 1019ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cAMP-dependent protein kinase activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. Ras protein signal transduction Source: SGD
  2. cell cycle Source: UniProtKB-KW
  3. cell division Source: UniProtKB-KW
  4. protein kinase A signaling Source: SGD
  5. protein phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

ATP-binding, cAMP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31604-MONOMER.
BRENDAi2.7.11.11. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type 1 (EC:2.7.11.11)
Short name:
PKA 1
Alternative name(s):
CDC25-suppressing protein kinase
PK-25
Gene namesi
Name:TPK1
Synonyms:PKA1, SRA3
Ordered Locus Names:YJL164C
ORF Names:J0541
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

SGDiS000003700. TPK1.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cAMP-dependent protein kinase complex Source: SGD
  2. cytoplasm Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 397396cAMP-dependent protein kinase type 1
PRO_0000086046Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP06244.
PaxDbiP06244.
PeptideAtlasiP06244.

Expressioni

Gene expression databases

GenevestigatoriP06244.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BCY1P072785EBI-9458,EBI-9475

Protein-protein interaction databases

BioGridi33595. 378 interactions.
DIPiDIP-548N.
IntActiP06244. 15 interactions.
MINTiMINT-569684.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi84 – 863
Beta strandi87 – 959
Beta strandi100 – 1067
Turni107 – 1093
Beta strandi112 – 1198
Helixi120 – 1256
Helixi129 – 14012
Beta strandi150 – 1556
Beta strandi157 – 1648
Helixi172 – 1787
Helixi184 – 20219
Turni203 – 2053
Helixi213 – 2153
Beta strandi216 – 2183
Beta strandi224 – 2263
Turni246 – 2483
Helixi251 – 2544
Helixi263 – 27715
Helixi287 – 29610
Helixi307 – 31610
Turni321 – 3233
Turni329 – 3324
Helixi333 – 3364
Helixi339 – 3413
Helixi346 – 3505
Helixi391 – 3933

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FOTX-ray2.80A81-397[»]
ProteinModelPortaliP06244.
SMRiP06244. Positions 51-397.

Miscellaneous databases

EvolutionaryTraceiP06244.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 341255Protein kinase
Add
BLAST
Domaini342 – 39756AGC-kinase C-terminal
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074358.
HOGENOMiHOG000233033.
KOiK04345.
OMAiFHEPDIT.
OrthoDBiEOG7R2BT5.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06244-1 [UniParc]FASTAAdd to Basket

« Hide

MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE    50
TQEKPKQPHV TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR 100
VHLIRSRHNG RYYAMKVLKK EIVVRLKQVE HTNDERLMLS IVTHPFIIRM 150
WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ RFPNPVAKFY AAEVCLALEY 200
LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY TLCGTPDYIA 250
PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR 300
FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL 350
SRNIETPYEP PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF 397
Length:397
Mass (Da):46,076
Last modified:September 21, 2011 - v3
Checksum:iD4E5C30D7F11458B
GO

Sequence cautioni

The sequence AAA35088.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 868SGKYSLQD → VGSIVYKN in AAA35088. 1 Publication
Sequence conflicti79 – 868SGKYSLQD → VGSIVYKN in CAA89459. 1 Publication
Sequence conflicti164 – 1718MDYIEGGE → ILKVER in AAA35088. 1 Publication
Sequence conflicti180 – 1812QR → KD in AAA35088. 1 Publication
Sequence conflicti188 – 1903KFY → QIF in AAA35088. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17072 Genomic DNA. Translation: AAA35164.1.
J02665 Genomic DNA. Translation: AAA34877.1.
M17224 Genomic DNA. Translation: AAA35088.1. Different initiation.
Z49439 Genomic DNA. Translation: CAA89459.1.
AY899257 mRNA. Translation: AAX83942.1.
BK006943 Genomic DNA. Translation: DAA08639.2.
PIRiJC1034. OKBYC1.
RefSeqiNP_012371.2. NM_001181597.2.

Genome annotation databases

EnsemblFungiiYJL164C; YJL164C; YJL164C.
GeneIDi853275.
KEGGisce:YJL164C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17072 Genomic DNA. Translation: AAA35164.1 .
J02665 Genomic DNA. Translation: AAA34877.1 .
M17224 Genomic DNA. Translation: AAA35088.1 . Different initiation.
Z49439 Genomic DNA. Translation: CAA89459.1 .
AY899257 mRNA. Translation: AAX83942.1 .
BK006943 Genomic DNA. Translation: DAA08639.2 .
PIRi JC1034. OKBYC1.
RefSeqi NP_012371.2. NM_001181597.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FOT X-ray 2.80 A 81-397 [» ]
ProteinModelPortali P06244.
SMRi P06244. Positions 51-397.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33595. 378 interactions.
DIPi DIP-548N.
IntActi P06244. 15 interactions.
MINTi MINT-569684.

Proteomic databases

MaxQBi P06244.
PaxDbi P06244.
PeptideAtlasi P06244.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL164C ; YJL164C ; YJL164C .
GeneIDi 853275.
KEGGi sce:YJL164C.

Organism-specific databases

SGDi S000003700. TPK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074358.
HOGENOMi HOG000233033.
KOi K04345.
OMAi FHEPDIT.
OrthoDBi EOG7R2BT5.

Enzyme and pathway databases

BioCyci YEAST:G3O-31604-MONOMER.
BRENDAi 2.7.11.11. 984.

Miscellaneous databases

EvolutionaryTracei P06244.
NextBioi 973556.
PROi P06244.

Gene expression databases

Genevestigatori P06244.

Family and domain databases

InterProi IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase."
    Toda T., Cameron S., Sass P., Zoller M., Wigler M.
    Cell 50:277-287(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Isolation and nucleotide sequence of a Saccharomyces cerevisiae protein kinase gene suppressing the cell cycle start mutation cdc25."
    Lisziewicz J., Godany A., Foerster H.-H., Kuentzel H.
    J. Biol. Chem. 262:2549-2553(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase."
    Cannon J.F., Tatchell K.
    Mol. Cell. Biol. 7:2653-2663(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 79-86.
    Strain: ATCC 204508 / S288c.
  6. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
    Zhang Z., Dietrich F.S.
    Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-82.
    Strain: ATCC 208353 / W303-1A.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  10. "Structure of the unliganded cAMP-dependent protein kinase catalytic subunit from Saccharomyces cerevisiae."
    Mashhoon N., Carmel G., Pflugrath J.W., Kuret J.
    Arch. Biochem. Biophys. 387:11-19(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 81-397.

Entry informationi

Entry nameiKAPA_YEAST
AccessioniPrimary (citable) accession number: P06244
Secondary accession number(s): D6VW23, P11595, Q2VQW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 159 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4320 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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