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P06244

- KAPA_YEAST

UniProt

P06244 - KAPA_YEAST

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Protein

cAMP-dependent protein kinase type 1

Gene

TPK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by cAMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161ATPPROSITE-ProRule annotation
Active sitei210 – 2101Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi93 – 1019ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cAMP-dependent protein kinase activity Source: SGD

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. protein kinase A signaling Source: SGD
  4. protein phosphorylation Source: SGD
  5. Ras protein signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

ATP-binding, cAMP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31604-MONOMER.
BRENDAi2.7.11.11. 984.
ReactomeiREACT_229657. PKA activation.
REACT_235985. Gluconeogenesis.
REACT_237720. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_237834. DARPP-32 events.
REACT_239269. PKA-mediated phosphorylation of key metabolic factors.
REACT_243583. PKA activation in glucagon signalling.
REACT_257022. PKA-mediated phosphorylation of CREB.
REACT_270118. Hedgehog 'off' state.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type 1 (EC:2.7.11.11)
Short name:
PKA 1
Alternative name(s):
CDC25-suppressing protein kinase
PK-25
Gene namesi
Name:TPK1
Synonyms:PKA1, SRA3
Ordered Locus Names:YJL164C
ORF Names:J0541
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

SGDiS000003700. TPK1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cAMP-dependent protein kinase complex Source: SGD
  2. cytoplasm Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 397396cAMP-dependent protein kinase type 1PRO_0000086046Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP06244.
PaxDbiP06244.
PeptideAtlasiP06244.

Expressioni

Gene expression databases

GenevestigatoriP06244.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BCY1P072785EBI-9458,EBI-9475

Protein-protein interaction databases

BioGridi33595. 378 interactions.
DIPiDIP-548N.
IntActiP06244. 15 interactions.
MINTiMINT-569684.

Structurei

Secondary structure

1
397
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi84 – 863Combined sources
Beta strandi87 – 959Combined sources
Beta strandi100 – 1067Combined sources
Turni107 – 1093Combined sources
Beta strandi112 – 1198Combined sources
Helixi120 – 1256Combined sources
Helixi129 – 14012Combined sources
Beta strandi150 – 1556Combined sources
Beta strandi157 – 1648Combined sources
Helixi172 – 1787Combined sources
Helixi184 – 20219Combined sources
Turni203 – 2053Combined sources
Helixi213 – 2153Combined sources
Beta strandi216 – 2183Combined sources
Beta strandi224 – 2263Combined sources
Turni246 – 2483Combined sources
Helixi251 – 2544Combined sources
Helixi263 – 27715Combined sources
Helixi287 – 29610Combined sources
Helixi307 – 31610Combined sources
Turni321 – 3233Combined sources
Turni329 – 3324Combined sources
Helixi333 – 3364Combined sources
Helixi339 – 3413Combined sources
Helixi346 – 3505Combined sources
Helixi391 – 3933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FOTX-ray2.80A81-397[»]
ProteinModelPortaliP06244.
SMRiP06244. Positions 51-397.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06244.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 341255Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini342 – 39756AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120454.
HOGENOMiHOG000233033.
InParanoidiP06244.
KOiK04345.
OMAiFHEPDIT.
OrthoDBiEOG7R2BT5.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06244-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE
60 70 80 90 100
TQEKPKQPHV TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR
110 120 130 140 150
VHLIRSRHNG RYYAMKVLKK EIVVRLKQVE HTNDERLMLS IVTHPFIIRM
160 170 180 190 200
WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ RFPNPVAKFY AAEVCLALEY
210 220 230 240 250
LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY TLCGTPDYIA
260 270 280 290 300
PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR
310 320 330 340 350
FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL
360 370 380 390
SRNIETPYEP PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF
Length:397
Mass (Da):46,076
Last modified:September 21, 2011 - v3
Checksum:iD4E5C30D7F11458B
GO

Sequence cautioni

The sequence AAA35088.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 868SGKYSLQD → VGSIVYKN in AAA35088. (PubMed:2823100)Curated
Sequence conflicti79 – 868SGKYSLQD → VGSIVYKN in CAA89459. (PubMed:8641269)Curated
Sequence conflicti164 – 1718MDYIEGGE → ILKVER in AAA35088. (PubMed:2823100)Curated
Sequence conflicti180 – 1812QR → KD in AAA35088. (PubMed:2823100)Curated
Sequence conflicti188 – 1903KFY → QIF in AAA35088. (PubMed:2823100)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17072 Genomic DNA. Translation: AAA35164.1.
J02665 Genomic DNA. Translation: AAA34877.1.
M17224 Genomic DNA. Translation: AAA35088.1. Different initiation.
Z49439 Genomic DNA. Translation: CAA89459.1.
AY899257 mRNA. Translation: AAX83942.1.
BK006943 Genomic DNA. Translation: DAA08639.2.
PIRiJC1034. OKBYC1.
RefSeqiNP_012371.2. NM_001181597.2.

Genome annotation databases

EnsemblFungiiYJL164C; YJL164C; YJL164C.
GeneIDi853275.
KEGGisce:YJL164C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17072 Genomic DNA. Translation: AAA35164.1 .
J02665 Genomic DNA. Translation: AAA34877.1 .
M17224 Genomic DNA. Translation: AAA35088.1 . Different initiation.
Z49439 Genomic DNA. Translation: CAA89459.1 .
AY899257 mRNA. Translation: AAX83942.1 .
BK006943 Genomic DNA. Translation: DAA08639.2 .
PIRi JC1034. OKBYC1.
RefSeqi NP_012371.2. NM_001181597.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FOT X-ray 2.80 A 81-397 [» ]
ProteinModelPortali P06244.
SMRi P06244. Positions 51-397.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33595. 378 interactions.
DIPi DIP-548N.
IntActi P06244. 15 interactions.
MINTi MINT-569684.

Proteomic databases

MaxQBi P06244.
PaxDbi P06244.
PeptideAtlasi P06244.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL164C ; YJL164C ; YJL164C .
GeneIDi 853275.
KEGGi sce:YJL164C.

Organism-specific databases

SGDi S000003700. TPK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00770000120454.
HOGENOMi HOG000233033.
InParanoidi P06244.
KOi K04345.
OMAi FHEPDIT.
OrthoDBi EOG7R2BT5.

Enzyme and pathway databases

BioCyci YEAST:G3O-31604-MONOMER.
BRENDAi 2.7.11.11. 984.
Reactomei REACT_229657. PKA activation.
REACT_235985. Gluconeogenesis.
REACT_237720. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_237834. DARPP-32 events.
REACT_239269. PKA-mediated phosphorylation of key metabolic factors.
REACT_243583. PKA activation in glucagon signalling.
REACT_257022. PKA-mediated phosphorylation of CREB.
REACT_270118. Hedgehog 'off' state.

Miscellaneous databases

EvolutionaryTracei P06244.
NextBioi 973556.
PROi P06244.

Gene expression databases

Genevestigatori P06244.

Family and domain databases

InterProi IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase."
    Toda T., Cameron S., Sass P., Zoller M., Wigler M.
    Cell 50:277-287(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Isolation and nucleotide sequence of a Saccharomyces cerevisiae protein kinase gene suppressing the cell cycle start mutation cdc25."
    Lisziewicz J., Godany A., Foerster H.-H., Kuentzel H.
    J. Biol. Chem. 262:2549-2553(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase."
    Cannon J.F., Tatchell K.
    Mol. Cell. Biol. 7:2653-2663(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 79-86.
    Strain: ATCC 204508 / S288c.
  6. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
    Zhang Z., Dietrich F.S.
    Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-82.
    Strain: ATCC 208353 / W303-1A.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  10. "Structure of the unliganded cAMP-dependent protein kinase catalytic subunit from Saccharomyces cerevisiae."
    Mashhoon N., Carmel G., Pflugrath J.W., Kuret J.
    Arch. Biochem. Biophys. 387:11-19(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 81-397.

Entry informationi

Entry nameiKAPA_YEAST
AccessioniPrimary (citable) accession number: P06244
Secondary accession number(s): D6VW23, P11595, Q2VQW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: September 21, 2011
Last modified: November 26, 2014
This is version 162 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4320 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3