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Protein

Cell division control protein 7

Gene

CDC7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase. Needed for the initiation of DNA synthesis during mitosis as well as for synaptonemal complex formation and commitment to recombination during meiosis. Required for HTA1-HTB1 locus transcription repression.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761ATPPROSITE-ProRule annotation
Active sitei163 – 1631Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 479ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • DNA replication initiation Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of exit from mitosis Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
  • positive regulation of meiosis I Source: SGD
  • positive regulation of meiotic DNA double-strand break formation Source: SGD
  • premeiotic DNA replication Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of chromatin silencing at telomere Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29446-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 7 (EC:2.7.11.1)
Gene namesi
Name:CDC7
Synonyms:OAF2
Ordered Locus Names:YDL017W
ORF Names:D2855
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDL017w.
EuPathDBiFungiDB:YDL017W.
SGDiS000002175. CDC7.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: SGD
  • Dbf4-dependent protein kinase complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507Cell division control protein 7PRO_0000085766Add
BLAST

Proteomic databases

MaxQBiP06243.
PaxDbiP06243.

Expressioni

Gene expression databases

GenevestigatoriP06243.

Interactioni

Subunit structurei

Associates with DBF4 and ORC2.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-4451,EBI-4451
CDC5P3256211EBI-4451,EBI-4440
DBF4P323257EBI-4451,EBI-5575
LRS4Q040875EBI-4451,EBI-32189

Protein-protein interaction databases

BioGridi32038. 204 interactions.
DIPiDIP-1235N.
IntActiP06243. 41 interactions.
MINTiMINT-385761.
STRINGi4932.YDL017W.

Structurei

3D structure databases

ProteinModelPortaliP06243.
SMRiP06243. Positions 16-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 469437Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000075011.
HOGENOMiHOG000193450.
InParanoidiP06243.
KOiK02214.
OMAiYVTSSPN.
OrthoDBiEOG7CVQ6R.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 3 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKTKNIDD IPPEIKEEMI QLYHDLPGIE NEYKLIDKIG EGTFSSVYKA
60 70 80 90 100
KDITGKITKK FASHFWNYGS NYVALKKIYV TSSPQRIYNE LNLLYIMTGS
110 120 130 140 150
SRVAPLCDAK RVRDQVIAVL PYYPHEEFRT FYRDLPIKGI KKYIWELLRA
160 170 180 190 200
LKFVHSKGII HRDIKPTNFL FNLELGRGVL VDFGLAEAQM DYKSMISSQN
210 220 230 240 250
DYDNYANTNH DGGYSMRNHE QFCPCIMRNQ YSPNSHNQTP PMVTIQNGKV
260 270 280 290 300
VHLNNVNGVD LTKGYPKNET RRIKRANRAG TRGFRAPEVL MKCGAQSTKI
310 320 330 340 350
DIWSVGVILL SLLGRRFPMF QSLDDADSLL ELCTIFGWKE LRKCAALHGL
360 370 380 390 400
GFEASGLIWD KPNGYSNGLK EFVYDLLNKE CTIGTFPEYS VAFETFGFLQ
410 420 430 440 450
QELHDRMSIE PQLPDPKTNM DAVDAYELKK YQEEIWSDHY WCFQVLEQCF
460 470 480 490 500
EMDPQKRSSA EDLLKTPFFN ELNENTYLLD GESTDEDDVV SSSEADLLDK

DVLLISE
Length:507
Mass (Da):58,320
Last modified:November 1, 1995 - v2
Checksum:iB74BA9144D0EC39F
GO

Sequence cautioni

The sequence CAA32370.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12624 Genomic DNA. Translation: AAA34485.1.
Z48432 Genomic DNA. Translation: CAA88342.1.
Z74065 Genomic DNA. Translation: CAA98574.1.
X14164 Genomic DNA. Translation: CAA32369.1.
X14164 Genomic DNA. Translation: CAA32370.1. Different initiation.
X15362 Genomic DNA. Translation: CAA33420.1.
BK006938 Genomic DNA. Translation: DAA11833.1.
PIRiA25228.
RefSeqiNP_010267.3. NM_001180076.3.

Genome annotation databases

EnsemblFungiiYDL017W; YDL017W; YDL017W.
GeneIDi851545.
KEGGisce:YDL017W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12624 Genomic DNA. Translation: AAA34485.1.
Z48432 Genomic DNA. Translation: CAA88342.1.
Z74065 Genomic DNA. Translation: CAA98574.1.
X14164 Genomic DNA. Translation: CAA32369.1.
X14164 Genomic DNA. Translation: CAA32370.1. Different initiation.
X15362 Genomic DNA. Translation: CAA33420.1.
BK006938 Genomic DNA. Translation: DAA11833.1.
PIRiA25228.
RefSeqiNP_010267.3. NM_001180076.3.

3D structure databases

ProteinModelPortaliP06243.
SMRiP06243. Positions 16-349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32038. 204 interactions.
DIPiDIP-1235N.
IntActiP06243. 41 interactions.
MINTiMINT-385761.
STRINGi4932.YDL017W.

Proteomic databases

MaxQBiP06243.
PaxDbiP06243.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL017W; YDL017W; YDL017W.
GeneIDi851545.
KEGGisce:YDL017W.

Organism-specific databases

CYGDiYDL017w.
EuPathDBiFungiDB:YDL017W.
SGDiS000002175. CDC7.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000075011.
HOGENOMiHOG000193450.
InParanoidiP06243.
KOiK02214.
OMAiYVTSSPN.
OrthoDBiEOG7CVQ6R.

Enzyme and pathway databases

BioCyciYEAST:G3O-29446-MONOMER.
BRENDAi2.7.11.1. 984.

Miscellaneous databases

NextBioi968958.
PROiP06243.

Gene expression databases

GenevestigatoriP06243.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 3 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of cell cycle gene CDC7 from Saccharomyces cerevisiae."
    Patterson M., Sclafani R.A., Fangman W.L., Rosamond J.
    Mol. Cell. Biol. 6:1590-1598(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Characterisation of the CDC7 gene product of Saccharomyces cerevisiae as a protein kinase needed for the initiation of mitotic DNA synthesis."
    Bahman M., Buck V., White A., Rosamond J.
    Biochim. Biophys. Acta 951:335-343(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-138.
  5. "Transcriptional analysis of the CDC7 protein kinase gene of Saccharomyces cerevisiae."
    Ham J., Moore D., Rosamond J., Johnston I.R.
    Nucleic Acids Res. 17:5781-5792(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
    Strain: ATCC 204508 / S288c.
  6. "CDC7 protein kinase activity is required for mitosis and meiosis in Saccharomyces cerevisiae."
    Buck V., Rosamond J.
    Mol. Gen. Genet. 227:452-457(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDC7_YEAST
AccessioniPrimary (citable) accession number: P06243
Secondary accession number(s): D6VRX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1995
Last modified: April 29, 2015
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.