Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P06243 (CDC7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell division control protein 7

EC=2.7.11.1
Gene names
Name:CDC7
Synonyms:OAF2
Ordered Locus Names:YDL017W
ORF Names:D2855
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine-protein kinase. Needed for the initiation of DNA synthesis during mitosis as well as for synaptonemal complex formation and commitment to recombination during meiosis. Required for HTA1-HTB1 locus transcription repression. Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Subunit structure

Associates with DBF4 and ORC2.

Miscellaneous

Present with 1600 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence CAA32370.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Meiosis
Mitosis
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from genetic interaction PubMed 23549044. Source: SGD

DNA replication initiation

Inferred from mutant phenotype PubMed 9472018. Source: SGD

double-strand break repair via break-induced replication

Inferred from mutant phenotype PubMed 20516198. Source: SGD

meiotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of exit from mitosis

Inferred from physical interaction PubMed 19478884. Source: SGD

protein phosphorylation

Inferred from mutant phenotype Ref.6PubMed 2023904PubMed 2166954. Source: SGD

regulation of chromatin silencing at telomere

Inferred from mutant phenotype PubMed 16980387. Source: SGD

   Cellular_componentDbf4-dependent protein kinase complex

Inferred from genetic interaction PubMed 8474449. Source: SGD

nucleus

Inferred from direct assay PubMed 2023904. Source: SGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

identical protein binding

Inferred from physical interaction PubMed 9790600. Source: IntAct

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine kinase activity

Inferred from mutant phenotype Ref.6PubMed 2023904PubMed 2166954. Source: SGD

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 507507Cell division control protein 7
PRO_0000085766

Regions

Domain33 – 469437Protein kinase
Nucleotide binding39 – 479ATP By similarity

Sites

Active site1631Proton acceptor By similarity
Binding site761ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P06243 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: B74BA9144D0EC39F

FASTA50758,320
        10         20         30         40         50         60 
MTSKTKNIDD IPPEIKEEMI QLYHDLPGIE NEYKLIDKIG EGTFSSVYKA KDITGKITKK 

        70         80         90        100        110        120 
FASHFWNYGS NYVALKKIYV TSSPQRIYNE LNLLYIMTGS SRVAPLCDAK RVRDQVIAVL 

       130        140        150        160        170        180 
PYYPHEEFRT FYRDLPIKGI KKYIWELLRA LKFVHSKGII HRDIKPTNFL FNLELGRGVL 

       190        200        210        220        230        240 
VDFGLAEAQM DYKSMISSQN DYDNYANTNH DGGYSMRNHE QFCPCIMRNQ YSPNSHNQTP 

       250        260        270        280        290        300 
PMVTIQNGKV VHLNNVNGVD LTKGYPKNET RRIKRANRAG TRGFRAPEVL MKCGAQSTKI 

       310        320        330        340        350        360 
DIWSVGVILL SLLGRRFPMF QSLDDADSLL ELCTIFGWKE LRKCAALHGL GFEASGLIWD 

       370        380        390        400        410        420 
KPNGYSNGLK EFVYDLLNKE CTIGTFPEYS VAFETFGFLQ QELHDRMSIE PQLPDPKTNM 

       430        440        450        460        470        480 
DAVDAYELKK YQEEIWSDHY WCFQVLEQCF EMDPQKRSSA EDLLKTPFFN ELNENTYLLD 

       490        500 
GESTDEDDVV SSSEADLLDK DVLLISE 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of cell cycle gene CDC7 from Saccharomyces cerevisiae."
Patterson M., Sclafani R.A., Fangman W.L., Rosamond J.
Mol. Cell. Biol. 6:1590-1598(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Characterisation of the CDC7 gene product of Saccharomyces cerevisiae as a protein kinase needed for the initiation of mitotic DNA synthesis."
Bahman M., Buck V., White A., Rosamond J.
Biochim. Biophys. Acta 951:335-343(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-138.
[5]"Transcriptional analysis of the CDC7 protein kinase gene of Saccharomyces cerevisiae."
Ham J., Moore D., Rosamond J., Johnston I.R.
Nucleic Acids Res. 17:5781-5792(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
Strain: ATCC 204508 / S288c.
[6]"CDC7 protein kinase activity is required for mitosis and meiosis in Saccharomyces cerevisiae."
Buck V., Rosamond J.
Mol. Gen. Genet. 227:452-457(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M12624 Genomic DNA. Translation: AAA34485.1.
Z48432 Genomic DNA. Translation: CAA88342.1.
Z74065 Genomic DNA. Translation: CAA98574.1.
X14164 Genomic DNA. Translation: CAA32369.1.
X14164 Genomic DNA. Translation: CAA32370.1. Different initiation.
X15362 Genomic DNA. Translation: CAA33420.1.
BK006938 Genomic DNA. Translation: DAA11833.1.
PIRA25228.
RefSeqNP_010267.3. NM_001180076.3.

3D structure databases

ProteinModelPortalP06243.
SMRP06243. Positions 13-472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32038. 202 interactions.
DIPDIP-1235N.
IntActP06243. 41 interactions.
MINTMINT-385761.
STRING4932.YDL017W.

Proteomic databases

PaxDbP06243.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL017W; YDL017W; YDL017W.
GeneID851545.
KEGGsce:YDL017W.

Organism-specific databases

CYGDYDL017w.
SGDS000002175. CDC7.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000075011.
HOGENOMHOG000193450.
KOK02214.
OMAYVTSSPN.
OrthoDBEOG7CVQ6R.

Enzyme and pathway databases

BioCycYEAST:G3O-29446-MONOMER.
BRENDA2.7.11.1. 984.

Gene expression databases

GenevestigatorP06243.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 2 hits.
[Graphical view]
SUPFAMSSF56112. SSF56112. 3 hits.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968958.
PROP06243.

Entry information

Entry nameCDC7_YEAST
AccessionPrimary (citable) accession number: P06243
Secondary accession number(s): D6VRX3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families