Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P06238 (A2MG_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-2-macroglobulin

Short name=Alpha-2-M
Gene names
Name:A2m
Synonyms:A2m1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1472 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Subunit structure

Homotetramer; disulfide-linked.

Subcellular location

Secreted.

Tissue specificity

Highest constitutive expression in ovary. Low level in testis, uterus and non-acute phase liver. Protein found in plasma. Ref.5

Induction

By inflammatory stimulus in liver. The level of this protein increases during acute phase, then decreases again. Ref.1

Sequence similarities

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727
Chain28 – 14721445Alpha-2-macroglobulin
PRO_0000000058

Regions

Region620 – 750131Bait region

Amino acid modifications

Glycosylation591N-linked (GlcNAc...) Potential
Glycosylation741N-linked (GlcNAc...) Potential
Glycosylation2501N-linked (GlcNAc...) Potential
Glycosylation3991N-linked (GlcNAc...) Potential
Glycosylation6511N-linked (GlcNAc...) Potential
Glycosylation7721N-linked (GlcNAc...) Potential
Glycosylation8671N-linked (GlcNAc...) Potential
Glycosylation9891N-linked (GlcNAc...) Potential
Glycosylation13641N-linked (GlcNAc...) Potential
Glycosylation14221N-linked (GlcNAc...) Potential
Glycosylation14261N-linked (GlcNAc...) Potential
Disulfide bond52 ↔ 90 By similarity
Disulfide bond254 ↔ 302 By similarity
Disulfide bond272 ↔ 290 By similarity
Disulfide bond281Interchain (with C-434) By similarity
Disulfide bond434Interchain (with C-281) By similarity
Disulfide bond473 ↔ 566 By similarity
Disulfide bond598 ↔ 769 By similarity
Disulfide bond647 ↔ 694 By similarity
Disulfide bond819 ↔ 847 By similarity
Disulfide bond845 ↔ 881 By similarity
Disulfide bond919 ↔ 1319 By similarity
Disulfide bond1077 ↔ 1125 By similarity
Disulfide bond1350 ↔ 1465 By similarity
Cross-link970 ↔ 973Isoglutamyl cysteine thioester (Cys-Gln) By similarity

Experimental info

Sequence conflict231L → V in CAA32164. Ref.3
Sequence conflict721R → H in AAA40636. Ref.1
Sequence conflict721R → H in CAA32164. Ref.3
Sequence conflict1031V → L in AAA40636. Ref.1
Sequence conflict1031V → L in CAA32164. Ref.3
Sequence conflict1201R → Q in AAA40636. Ref.1
Sequence conflict1201R → Q in CAA32164. Ref.3
Sequence conflict4901M → L in AAA40638. Ref.4
Sequence conflict10251T → A in AAA40636. Ref.1
Sequence conflict11921A → G in AAA40636. Ref.1
Sequence conflict12001P → T in AAA40636. Ref.1
Sequence conflict12791H → R in AAA40636. Ref.1
Sequence conflict13401T → A in AAA40636. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P06238 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: BFD63B8A4F6A2632

FASTA1,472163,785
        10         20         30         40         50         60 
MGKHRLRSLA LLPLLLRLLL LLLPTDASAP QKPIYMVMVP SLLHAGTPEK ACFLFSHLNE 

        70         80         90        100        110        120 
TVAVRVSLES VRGNQSLFTD LVVDKDLFHC TSFTVPQSSS DEVMFFTVQV KGATHEFRRR 

       130        140        150        160        170        180 
STVLVKKKES LVFAQTDKPI YKPGQTVRFR VVSLDESFHP LNELIPLLYI QDPKNNRIAQ 

       190        200        210        220        230        240 
WQNFNLEGGL KQLSFPLSSE PTQGSYKVVI RTESGRTVEH PFSVEEFVLP KFEVRVTVPE 

       250        260        270        280        290        300 
TITILEEEMN VSVCGIYTYG KPVPGRVTVN ICRKYSNPSN CFGEESVAFC EKLSQQLDGR 

       310        320        330        340        350        360 
GCFSQLVKTK SFQLKRQEYE MQLDVHAKIQ EEGTGVEETG KGLTKITRTI TKLSFVNVDS 

       370        380        390        400        410        420 
HFRQGIPFVG QVLLVDGRGT PIPYETIFIG ADEANLYINT TTDKHGLARF SINTDDIMGT 

       430        440        450        460        470        480 
SLTVRAKYKD SNACYGFRWL TEENVEAWHT AYAVFSPSRS FLHLESLPDK LRCDQTLEVQ 

       490        500        510        520        530        540 
AHYILNGEAM QELKELVFYY LMMAKGGIVR AGTHVLPLKQ GQMRGHFSIL ISMETDLAPV 

       550        560        570        580        590        600 
ARLVLYAILP NGEVVGDTAK YEIENCLANK VDLVFRPNSG LPATRALLSV MASPQSLCGL 

       610        620        630        640        650        660 
RAVDQSVLLM KPETELSASL IYDLLPVKDL TGFPQGADQR EEDTNGCVKQ NDTYINGILY 

       670        680        690        700        710        720 
SPVQNTNEED MYGFLKDMGL KVFTNSNIRK PKVCERLRDN KGIPAAYHLV SQSHMDAFLE 

       730        740        750        760        770        780 
SSESPTETRR SYFPETWIWD LVVVDSAGVA EVEVTVPDTI TEWKAGAFCL SNDTGLGLSP 

       790        800        810        820        830        840 
VVQFQAFQPF FVELTMPYSV IRGEAFTLKA TVLNYLPTCI RVAVQLEASP DFLAAPEEKE 

       850        860        870        880        890        900 
QRSHCICMNQ RHTASWAVIP KSLGNVNFTV SAEALNSKEL CGNEVPVVPE QGKKDTIIKS 

       910        920        930        940        950        960 
LLVEPEGLEN EVTFNSLLCP MGAEVSELIA LKLPSDVVEE SARASVTVLG DILGSAMQNT 

       970        980        990       1000       1010       1020 
QDLLKMPYGC GEQNMVLFAP NIYVLDYLNE TQQLTQEIKT KAIAYLNTGY QRQLNYKHRD 

      1030       1040       1050       1060       1070       1080 
GSYSTFGDKP GRNHANTWLT AFVLKSFAQA RKYIFIDEVH ITQALLWLSQ QQKDNGCFRS 

      1090       1100       1110       1120       1130       1140 
SGSLLNNAMK GGVEDEVTLS AYITIALLEM SLPVTHPVVR NALFCLDTAW KSARGGAGGS 

      1150       1160       1170       1180       1190       1200 
HVYTKALLAY AFALAGNQDT KKEILKSLDE EAVKEEDSVH WTRPQKPSVS VALWYQPQAP 

      1210       1220       1230       1240       1250       1260 
SAEVEMTAYV LLAYLTTEPA PTQEDLTAAM LIVKWLTKQQ NSHGGFSSTQ DTVVALHALS 

      1270       1280       1290       1300       1310       1320 
KYGSATFTRA KKAAQVTIHS SGTFSTKFQV NNNNQLLLQR VTLPTVPGDY TVKVTGEGCV 

      1330       1340       1350       1360       1370       1380 
YLQTSLKYSV LPREEEFPFT VVVQTLPGTC EDPKAHTSFQ ISLNISYTGS RSESNMAIAD 

      1390       1400       1410       1420       1430       1440 
VKMVSGFIPL KPTVKMLERS VHVSRTEVSN NHVLIYLDKV SNQTVNLSFT VQQDIPIRDL 

      1450       1460       1470 
KPAVVKVYDY YEKDEFAVAK YSAPCSTDYG NA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of rat liver alpha 2-macroglobulin and acute phase control of its messenger RNA."
Gehring M.R., Shiels B.R., Northemann W., de Bruijn M.H.L., Kan C.-C., Chain A.C., Noonan D.J., Fey G.H.
J. Biol. Chem. 262:446-454(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[3]"Identification of the promoter sequences involved in the interleukin-6 dependent expression of the rat alpha 2-macroglobulin gene."
Kunz D., Zimmermann R., Heisig M., Heinrich P.C.
Nucleic Acids Res. 17:1121-1138(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-164.
Strain: Wistar.
Tissue: Liver.
[4]"Molecular cloning of DNA complementary to rat alpha 2-macroglobulin mRNA."
Hayashida K., Okubo H., Noguchi M., Yoshida H., Kangawa K., Matsuo H., Sakaki Y.
J. Biol. Chem. 260:14224-14229(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 178-227 AND 420-526.
[5]"Sequence of rat alpha 1-macroglobulin, a broad-range proteinase inhibitor from the alpha-macroglobulin-complement family."
Eggertsen G., Hudson G., Shiels B., Reed D., Fey G.H.
Mol. Biol. Med. 8:287-302(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
Strain: Fischer 344.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02635 mRNA. Translation: AAA40636.1.
BC098922 mRNA. Translation: AAH98922.1.
X13983, X13984, X13985 Genomic DNA. Translation: CAA32164.1.
M11792 mRNA. Translation: AAA40637.1.
M11793 mRNA. Translation: AAA40638.1.
PIRA26122.
RefSeqNP_036620.2. NM_012488.2.
XP_003749876.1. XM_003749828.2.
UniGeneRn.225884.

3D structure databases

ProteinModelPortalP06238.
SMRP06238. Positions 129-230, 1336-1472.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI39.001.

Proteomic databases

PaxDbP06238.
PRIDEP06238.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000019346; ENSRNOP00000019346; ENSRNOG00000028896.
GeneID100911545.
24153.
KEGGrno:100911545.
rno:24153.
UCSCRGD:2004. rat.

Organism-specific databases

CTD2.
RGD2004. A2m.

Phylogenomic databases

eggNOGCOG2373.
GeneTreeENSGT00740000115177.
HOGENOMHOG000220939.
HOVERGENHBG000039.
KOK03910.
OMAQTVQAHY.
OrthoDBEOG7DJSKB.
PhylomeDBP06238.
TreeFamTF313285.

Gene expression databases

GenevestigatorP06238.

Family and domain databases

Gene3D1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602439.

Entry information

Entry nameA2MG_RAT
AccessionPrimary (citable) accession number: P06238
Secondary accession number(s): Q4FZY3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families