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Protein

RNA polymerase sigma factor SigA

Gene

sigA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.UniRule annotation

Miscellaneous

A conformational change of sigma-A occurs during its association with the core enzyme.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi332 – 351H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Sigma factor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciBSUB:BSU25200-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor SigAUniRule annotation
Alternative name(s):
Sigma-43
Sigma-A
Gene namesi
Name:sigAUniRule annotation
Synonyms:rpoD
Ordered Locus Names:BSU25200
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938741 – 371RNA polymerase sigma factor SigAAdd BLAST371

Proteomic databases

PaxDbiP06224
PRIDEiP06224

Interactioni

Subunit structurei

Interacts transiently with the RNA polymerase catalytic core.UniRule annotation

Protein-protein interaction databases

IntActiP06224, 2 interactors
STRINGi224308.Bsubs1_010100013786

Structurei

Secondary structure

1371
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 27Combined sources15
Beta strandi28 – 31Combined sources4
Helixi32 – 38Combined sources7
Helixi39 – 41Combined sources3
Helixi46 – 59Combined sources14
Beta strandi62 – 64Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MWWNMR-A2-72[»]
ProteinModelPortaliP06224
SMRiP06224
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni138 – 208Sigma-70 factor domain-2UniRule annotationAdd BLAST71
Regioni217 – 293Sigma-70 factor domain-3UniRule annotationAdd BLAST77
Regioni306 – 359Sigma-70 factor domain-4UniRule annotationAdd BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi162 – 165Interaction with polymerase core subunit RpoC4

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoD/SigA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DG1 Bacteria
COG0568 LUCA
HOGENOMiHOG000270273
InParanoidiP06224
KOiK03086
OMAiYQRWLAE
PhylomeDBiP06224

Family and domain databases

Gene3Di1.10.10.10, 2 hits
HAMAPiMF_00963 Sigma70_RpoD_SigA, 1 hit
InterProiView protein in InterPro
IPR014284 RNA_pol_sigma-70_dom
IPR000943 RNA_pol_sigma70
IPR009042 RNA_pol_sigma70_r1_2
IPR007627 RNA_pol_sigma70_r2
IPR007624 RNA_pol_sigma70_r3
IPR007630 RNA_pol_sigma70_r4
IPR007127 RNA_pol_sigma_70_r1_1
IPR013325 RNA_pol_sigma_r2
IPR013324 RNA_pol_sigma_r3/r4-like
IPR012760 RNA_pol_sigma_RpoD_C
IPR028630 Sigma70_RpoD
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF03979 Sigma70_r1_1, 1 hit
PF00140 Sigma70_r1_2, 1 hit
PF04542 Sigma70_r2, 1 hit
PF04539 Sigma70_r3, 1 hit
PF04545 Sigma70_r4, 1 hit
PRINTSiPR00046 SIGMA70FCT
SUPFAMiSSF88659 SSF88659, 2 hits
SSF88946 SSF88946, 1 hit
TIGRFAMsiTIGR02393 RpoD_Cterm, 1 hit
TIGR02937 sigma70-ECF, 1 hit
PROSITEiView protein in PROSITE
PS00715 SIGMA70_1, 1 hit
PS00716 SIGMA70_2, 1 hit

Sequencei

Sequence statusi: Complete.

P06224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKQTHETE LTFDQVKEQL TESGKKRGVL TYEEIAERMS SFEIESDQMD
60 70 80 90 100
EYYEFLGEQG VELISENEET EDPNIQQLAK AEEEFDLNDL SVPPGVKIND
110 120 130 140 150
PVRMYLKEIG RVNLLSAKEE IAYAQKIEEG DEESKRRLAE ANLRLVVSIA
160 170 180 190 200
KRYVGRGMLF LDLIQEGNMG LMKAVEKFDY RKGYKFSTYA TWWIRQAITR
210 220 230 240 250
AIADQARTIR IPVHMVETIN KLIRVQRQLL QDLGREPTPE EIAEDMDLTP
260 270 280 290 300
EKVREILKIA QEPVSLETPI GEEDDSHLGD FIEDQEATSP SDHAAYELLK
310 320 330 340 350
EQLEDVLDTL TDREENVLRL RFGLDDGRTR TLEEVGKVFG VTRERIRQIE
360 370
AKALRKLRHP SRSKRLKDFL E
Length:371
Mass (Da):42,948
Last modified:July 7, 2009 - v2
Checksum:i71EF04508FBF6471
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165Q → H in AAA22709 (PubMed:2987246).Curated1
Sequence conflicti165Q → H in CAA27538 (PubMed:3086839).Curated1
Sequence conflicti165Q → H in BAA12489 (PubMed:8969508).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10089 Genomic DNA Translation: AAA22709.1
X03897 Genomic DNA Translation: CAA27538.1
D84432 Genomic DNA Translation: BAA12489.1
AL009126 Genomic DNA Translation: CAB14450.2
J05569 Genomic DNA No translation available.
PIRiA22626 RNBS43
RefSeqiNP_390399.2, NC_000964.3
WP_003226225.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14450; CAB14450; BSU25200
GeneIDi937897
KEGGibsu:BSU25200
PATRICifig|224308.179.peg.2740

Entry informationi

Entry nameiSIGA_BACSU
AccessioniPrimary (citable) accession number: P06224
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: July 7, 2009
Last modified: April 25, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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