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Protein

RNA polymerase sigma factor SigA

Gene

sigA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi332 – 35120H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Sigma factor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU25200-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor SigAUniRule annotation
Alternative name(s):
Sigma-43
Sigma-A
Gene namesi
Name:sigAUniRule annotation
Synonyms:rpoD
Ordered Locus Names:BSU25200
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 371371RNA polymerase sigma factor SigAPRO_0000093874Add
BLAST

Proteomic databases

PaxDbiP06224.

Interactioni

Subunit structurei

Interacts transiently with the RNA polymerase catalytic core.UniRule annotation

Protein-protein interaction databases

IntActiP06224. 2 interactions.
STRINGi224308.Bsubs1_010100013786.

Structurei

3D structure databases

ProteinModelPortaliP06224.
SMRiP06224. Positions 8-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni138 – 20871Sigma-70 factor domain-2UniRule annotationAdd
BLAST
Regioni217 – 29377Sigma-70 factor domain-3UniRule annotationAdd
BLAST
Regioni306 – 35954Sigma-70 factor domain-4UniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi162 – 1654Interaction with polymerase core subunit RpoC

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoD/SigA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DG1. Bacteria.
COG0568. LUCA.
HOGENOMiHOG000270273.
InParanoidiP06224.
KOiK03086.
OMAiDTMKISG.
PhylomeDBiP06224.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_00963. Sigma70_RpoD_SigA. 1 hit.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR007127. RNA_pol_sigma_70_r1_1.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012760. RNA_pol_sigma_RpoD_C.
IPR028630. Sigma70_RpoD.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03979. Sigma70_r1_1. 1 hit.
PF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02393. RpoD_Cterm. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKQTHETE LTFDQVKEQL TESGKKRGVL TYEEIAERMS SFEIESDQMD
60 70 80 90 100
EYYEFLGEQG VELISENEET EDPNIQQLAK AEEEFDLNDL SVPPGVKIND
110 120 130 140 150
PVRMYLKEIG RVNLLSAKEE IAYAQKIEEG DEESKRRLAE ANLRLVVSIA
160 170 180 190 200
KRYVGRGMLF LDLIQEGNMG LMKAVEKFDY RKGYKFSTYA TWWIRQAITR
210 220 230 240 250
AIADQARTIR IPVHMVETIN KLIRVQRQLL QDLGREPTPE EIAEDMDLTP
260 270 280 290 300
EKVREILKIA QEPVSLETPI GEEDDSHLGD FIEDQEATSP SDHAAYELLK
310 320 330 340 350
EQLEDVLDTL TDREENVLRL RFGLDDGRTR TLEEVGKVFG VTRERIRQIE
360 370
AKALRKLRHP SRSKRLKDFL E
Length:371
Mass (Da):42,948
Last modified:July 7, 2009 - v2
Checksum:i71EF04508FBF6471
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti165 – 1651Q → H in AAA22709 (PubMed:2987246).Curated
Sequence conflicti165 – 1651Q → H in CAA27538 (PubMed:3086839).Curated
Sequence conflicti165 – 1651Q → H in BAA12489 (PubMed:8969508).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10089 Genomic DNA. Translation: AAA22709.1.
X03897 Genomic DNA. Translation: CAA27538.1.
D84432 Genomic DNA. Translation: BAA12489.1.
AL009126 Genomic DNA. Translation: CAB14450.2.
J05569 Genomic DNA. No translation available.
PIRiA22626. RNBS43.
RefSeqiNP_390399.2. NC_000964.3.
WP_003226225.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14450; CAB14450; BSU25200.
GeneIDi11240188.
937897.
KEGGibsu:BSU25200.
PATRICi18976878. VBIBacSub10457_2628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10089 Genomic DNA. Translation: AAA22709.1.
X03897 Genomic DNA. Translation: CAA27538.1.
D84432 Genomic DNA. Translation: BAA12489.1.
AL009126 Genomic DNA. Translation: CAB14450.2.
J05569 Genomic DNA. No translation available.
PIRiA22626. RNBS43.
RefSeqiNP_390399.2. NC_000964.3.
WP_003226225.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP06224.
SMRiP06224. Positions 8-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP06224. 2 interactions.
STRINGi224308.Bsubs1_010100013786.

Proteomic databases

PaxDbiP06224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14450; CAB14450; BSU25200.
GeneIDi11240188.
937897.
KEGGibsu:BSU25200.
PATRICi18976878. VBIBacSub10457_2628.

Phylogenomic databases

eggNOGiENOG4105DG1. Bacteria.
COG0568. LUCA.
HOGENOMiHOG000270273.
InParanoidiP06224.
KOiK03086.
OMAiDTMKISG.
PhylomeDBiP06224.

Enzyme and pathway databases

BioCyciBSUB:BSU25200-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_00963. Sigma70_RpoD_SigA. 1 hit.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR007127. RNA_pol_sigma_70_r1_1.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012760. RNA_pol_sigma_RpoD_C.
IPR028630. Sigma70_RpoD.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03979. Sigma70_r1_1. 1 hit.
PF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02393. RpoD_Cterm. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIGA_BACSU
AccessioniPrimary (citable) accession number: P06224
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: July 7, 2009
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A conformational change of sigma-A occurs during its association with the core enzyme.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.