SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P06219

- BGAL1_KLEPN

UniProt

P06219 - BGAL1_KLEPN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Beta-galactosidase
Gene
lacZ
Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei108 – 1081Substrate By similarity
Metal bindingi207 – 2071Sodium By similarity
Binding sitei207 – 2071Substrate By similarity
Sitei364 – 3641Transition state stabilizer By similarity
Sitei398 – 3981Transition state stabilizer By similarity
Metal bindingi423 – 4231Magnesium 1 By similarity
Metal bindingi425 – 4251Magnesium 1 By similarity
Active sitei468 – 4681Proton donor By similarity
Metal bindingi468 – 4681Magnesium 1 By similarity
Binding sitei468 – 4681Substrate By similarity
Active sitei544 – 5441Nucleophile By similarity
Metal bindingi604 – 6041Magnesium 2 By similarity
Metal bindingi608 – 6081Sodium; via carbonyl oxygen By similarity
Metal bindingi611 – 6111Sodium By similarity
Binding sitei611 – 6111Substrate By similarity
Binding sitei1010 – 10101Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10341034Beta-galactosidaseUniRule annotation
PRO_0000057662Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP06219.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni544 – 5474Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06219-1 [UniParc]FASTAAdd to Basket

« Hide

MQISDTGRSH TPDFHAVLAR EDWHNQTITH LNRLPAHPVF ASWRDELAAR     50
DNLPSSRRRQ LDGSGSSLTP AARLPSMRVV TQDLPDCRGT PVPSNWQMEG 100
YDAPIYTNVR YPIDTTPPRV PEDNPTGCYS LHFTVEDTWR ENGQTQIIFD 150
GVNSAFHLWC NGVWVGYSQD SRLPAAFDLS PFLRPGDNRL CVMVMRWSAG 200
SWLEDQDMWR MSGIFRSVWL LNKPQQRLCD VQLTPALDAL YRDGTLQVQA 250
TIEATEAALA GLSVGVSLWR GEEQFAAGRQ PLGTPTVDER GHYAERVDFS 300
LAVATPAHWS AETPNCYRAV VTLWRGDELL EAEAWDIGFR RIEIADGLLR 350
LNGKPLLIRG VNRHEHHHLR GQVVTEADMV QDILLMKQNN FNAVRCSHYP 400
NAPRWYELCN RYGLYVVDEA NIETHGMVPM NRLSDDPAWL PAFSARVTRM 450
VQSNRNHPCI IIWSLGNESG GGGNHEALYH WLKRNDPSRP VQYEGGGADT 500
TATDIICPMY ARVERDQPIP AVPKWGIKKW ISLPGEQRPL ILCEYAHAMG 550
NSLGNFADYW QAFREYPRLQ GGFIWDWADQ AIRKTFADGS VGWAYGGDFG 600
DKPNDRQFCM NGLVFPDRTP HPSLVEAKHA QQYFQFTLLS TSPLRVRIIS 650
EYLFRPTDNE VVRWQVQAAG EPLYHGDLTL ALPPEGSDEI TLLDSLILPE 700
GARAVWLTLE VTQPQATAWS EAEHRVAWQQ FPLPAPLGCR RPPCLPALPD 750
LIVSDEVWQI RAGSQCWTID RRTGLLSRWS VGGQEQLLTP LRDQFIRAPL 800
DNDIGVSEVE RIDPNAWVER WRSAGLYDLE AHCVQCDAQR LANETLVDCR 850
WHYLRGEEVV IVSHWRMHFT ADGTLRLAVD GERAETLPPL PRVGLHFQVA 900
DQQAPVSWLG LGPHENYPDR RSSACFARWE QPLAAMTTPY IFPTENGLRC 950
DTQALDWGRW HISGHFHFSV QPWSTRQLME TDHWHKMQAE DGVWITLDGL 1000
HMGVGGDDSW TPSVLPQWLL SQTRWQYEVS LRSL 1034
Length:1,034
Mass (Da):117,517
Last modified:January 1, 1988 - v1
Checksum:i8F8B9DC7521EF649
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11441 Genomic DNA. Translation: AAA25082.1.
PIRiA24925.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11441 Genomic DNA. Translation: AAA25082.1 .
PIRi A24925.

3D structure databases

ProteinModelPortali P06219.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of Klebsiella pneumoniae lac genes."
    Buvinger W.E., Riley M.
    J. Bacteriol. 163:850-857(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiBGAL1_KLEPN
AccessioniPrimary (citable) accession number: P06219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi