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P06219

- BGAL1_KLEPN

UniProt

P06219 - BGAL1_KLEPN

Protein

Beta-galactosidase

Gene

lacZ

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei108 – 1081SubstrateUniRule annotation
    Metal bindingi207 – 2071SodiumUniRule annotation
    Binding sitei207 – 2071SubstrateUniRule annotation
    Sitei364 – 3641Transition state stabilizerUniRule annotation
    Sitei398 – 3981Transition state stabilizerUniRule annotation
    Metal bindingi423 – 4231Magnesium 1UniRule annotation
    Metal bindingi425 – 4251Magnesium 1UniRule annotation
    Active sitei468 – 4681Proton donorUniRule annotation
    Metal bindingi468 – 4681Magnesium 1UniRule annotation
    Binding sitei468 – 4681SubstrateUniRule annotation
    Active sitei544 – 5441NucleophileUniRule annotation
    Metal bindingi604 – 6041Magnesium 2UniRule annotation
    Metal bindingi608 – 6081Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi611 – 6111SodiumUniRule annotation
    Binding sitei611 – 6111SubstrateUniRule annotation
    Binding sitei1010 – 10101SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    OrganismiKlebsiella pneumoniae
    Taxonomic identifieri573 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10341034Beta-galactosidasePRO_0000057662Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    3D structure databases

    ProteinModelPortaliP06219.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni544 – 5474Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P06219-1 [UniParc]FASTAAdd to Basket

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    MQISDTGRSH TPDFHAVLAR EDWHNQTITH LNRLPAHPVF ASWRDELAAR     50
    DNLPSSRRRQ LDGSGSSLTP AARLPSMRVV TQDLPDCRGT PVPSNWQMEG 100
    YDAPIYTNVR YPIDTTPPRV PEDNPTGCYS LHFTVEDTWR ENGQTQIIFD 150
    GVNSAFHLWC NGVWVGYSQD SRLPAAFDLS PFLRPGDNRL CVMVMRWSAG 200
    SWLEDQDMWR MSGIFRSVWL LNKPQQRLCD VQLTPALDAL YRDGTLQVQA 250
    TIEATEAALA GLSVGVSLWR GEEQFAAGRQ PLGTPTVDER GHYAERVDFS 300
    LAVATPAHWS AETPNCYRAV VTLWRGDELL EAEAWDIGFR RIEIADGLLR 350
    LNGKPLLIRG VNRHEHHHLR GQVVTEADMV QDILLMKQNN FNAVRCSHYP 400
    NAPRWYELCN RYGLYVVDEA NIETHGMVPM NRLSDDPAWL PAFSARVTRM 450
    VQSNRNHPCI IIWSLGNESG GGGNHEALYH WLKRNDPSRP VQYEGGGADT 500
    TATDIICPMY ARVERDQPIP AVPKWGIKKW ISLPGEQRPL ILCEYAHAMG 550
    NSLGNFADYW QAFREYPRLQ GGFIWDWADQ AIRKTFADGS VGWAYGGDFG 600
    DKPNDRQFCM NGLVFPDRTP HPSLVEAKHA QQYFQFTLLS TSPLRVRIIS 650
    EYLFRPTDNE VVRWQVQAAG EPLYHGDLTL ALPPEGSDEI TLLDSLILPE 700
    GARAVWLTLE VTQPQATAWS EAEHRVAWQQ FPLPAPLGCR RPPCLPALPD 750
    LIVSDEVWQI RAGSQCWTID RRTGLLSRWS VGGQEQLLTP LRDQFIRAPL 800
    DNDIGVSEVE RIDPNAWVER WRSAGLYDLE AHCVQCDAQR LANETLVDCR 850
    WHYLRGEEVV IVSHWRMHFT ADGTLRLAVD GERAETLPPL PRVGLHFQVA 900
    DQQAPVSWLG LGPHENYPDR RSSACFARWE QPLAAMTTPY IFPTENGLRC 950
    DTQALDWGRW HISGHFHFSV QPWSTRQLME TDHWHKMQAE DGVWITLDGL 1000
    HMGVGGDDSW TPSVLPQWLL SQTRWQYEVS LRSL 1034
    Length:1,034
    Mass (Da):117,517
    Last modified:January 1, 1988 - v1
    Checksum:i8F8B9DC7521EF649
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11441 Genomic DNA. Translation: AAA25082.1.
    PIRiA24925.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11441 Genomic DNA. Translation: AAA25082.1 .
    PIRi A24925.

    3D structure databases

    ProteinModelPortali P06219.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of Klebsiella pneumoniae lac genes."
      Buvinger W.E., Riley M.
      J. Bacteriol. 163:850-857(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiBGAL1_KLEPN
    AccessioniPrimary (citable) accession number: P06219
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3