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P06219

- BGAL1_KLEPN

UniProt

P06219 - BGAL1_KLEPN

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer.UniRule annotation
Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei108 – 1081SubstrateUniRule annotation
Metal bindingi207 – 2071SodiumUniRule annotation
Binding sitei207 – 2071SubstrateUniRule annotation
Sitei364 – 3641Transition state stabilizerUniRule annotation
Sitei398 – 3981Transition state stabilizerUniRule annotation
Metal bindingi423 – 4231Magnesium 1UniRule annotation
Metal bindingi425 – 4251Magnesium 1UniRule annotation
Active sitei468 – 4681Proton donorUniRule annotation
Metal bindingi468 – 4681Magnesium 1UniRule annotation
Binding sitei468 – 4681SubstrateUniRule annotation
Active sitei544 – 5441NucleophileUniRule annotation
Metal bindingi604 – 6041Magnesium 2UniRule annotation
Metal bindingi608 – 6081Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi611 – 6111SodiumUniRule annotation
Binding sitei611 – 6111SubstrateUniRule annotation
Binding sitei1010 – 10101SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10341034Beta-galactosidasePRO_0000057662Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP06219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni544 – 5474Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06219 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQISDTGRSH TPDFHAVLAR EDWHNQTITH LNRLPAHPVF ASWRDELAAR
60 70 80 90 100
DNLPSSRRRQ LDGSGSSLTP AARLPSMRVV TQDLPDCRGT PVPSNWQMEG
110 120 130 140 150
YDAPIYTNVR YPIDTTPPRV PEDNPTGCYS LHFTVEDTWR ENGQTQIIFD
160 170 180 190 200
GVNSAFHLWC NGVWVGYSQD SRLPAAFDLS PFLRPGDNRL CVMVMRWSAG
210 220 230 240 250
SWLEDQDMWR MSGIFRSVWL LNKPQQRLCD VQLTPALDAL YRDGTLQVQA
260 270 280 290 300
TIEATEAALA GLSVGVSLWR GEEQFAAGRQ PLGTPTVDER GHYAERVDFS
310 320 330 340 350
LAVATPAHWS AETPNCYRAV VTLWRGDELL EAEAWDIGFR RIEIADGLLR
360 370 380 390 400
LNGKPLLIRG VNRHEHHHLR GQVVTEADMV QDILLMKQNN FNAVRCSHYP
410 420 430 440 450
NAPRWYELCN RYGLYVVDEA NIETHGMVPM NRLSDDPAWL PAFSARVTRM
460 470 480 490 500
VQSNRNHPCI IIWSLGNESG GGGNHEALYH WLKRNDPSRP VQYEGGGADT
510 520 530 540 550
TATDIICPMY ARVERDQPIP AVPKWGIKKW ISLPGEQRPL ILCEYAHAMG
560 570 580 590 600
NSLGNFADYW QAFREYPRLQ GGFIWDWADQ AIRKTFADGS VGWAYGGDFG
610 620 630 640 650
DKPNDRQFCM NGLVFPDRTP HPSLVEAKHA QQYFQFTLLS TSPLRVRIIS
660 670 680 690 700
EYLFRPTDNE VVRWQVQAAG EPLYHGDLTL ALPPEGSDEI TLLDSLILPE
710 720 730 740 750
GARAVWLTLE VTQPQATAWS EAEHRVAWQQ FPLPAPLGCR RPPCLPALPD
760 770 780 790 800
LIVSDEVWQI RAGSQCWTID RRTGLLSRWS VGGQEQLLTP LRDQFIRAPL
810 820 830 840 850
DNDIGVSEVE RIDPNAWVER WRSAGLYDLE AHCVQCDAQR LANETLVDCR
860 870 880 890 900
WHYLRGEEVV IVSHWRMHFT ADGTLRLAVD GERAETLPPL PRVGLHFQVA
910 920 930 940 950
DQQAPVSWLG LGPHENYPDR RSSACFARWE QPLAAMTTPY IFPTENGLRC
960 970 980 990 1000
DTQALDWGRW HISGHFHFSV QPWSTRQLME TDHWHKMQAE DGVWITLDGL
1010 1020 1030
HMGVGGDDSW TPSVLPQWLL SQTRWQYEVS LRSL
Length:1,034
Mass (Da):117,517
Last modified:January 1, 1988 - v1
Checksum:i8F8B9DC7521EF649
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11441 Genomic DNA. Translation: AAA25082.1.
PIRiA24925.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11441 Genomic DNA. Translation: AAA25082.1 .
PIRi A24925.

3D structure databases

ProteinModelPortali P06219.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of Klebsiella pneumoniae lac genes."
    Buvinger W.E., Riley M.
    J. Bacteriol. 163:850-857(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiBGAL1_KLEPN
AccessioniPrimary (citable) accession number: P06219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 1, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3