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P06219 (BGAL1_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length1034 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10341034Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000057662

Regions

Region544 – 5474Substrate binding By similarity

Sites

Active site4681Proton donor By similarity
Active site5441Nucleophile By similarity
Metal binding2071Sodium By similarity
Metal binding4231Magnesium 1 By similarity
Metal binding4251Magnesium 1 By similarity
Metal binding4681Magnesium 1 By similarity
Metal binding6041Magnesium 2 By similarity
Metal binding6081Sodium; via carbonyl oxygen By similarity
Metal binding6111Sodium By similarity
Binding site1081Substrate By similarity
Binding site2071Substrate By similarity
Binding site4681Substrate By similarity
Binding site6111Substrate By similarity
Binding site10101Substrate By similarity
Site3641Transition state stabilizer By similarity
Site3981Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
P06219 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 8F8B9DC7521EF649

FASTA1,034117,517
        10         20         30         40         50         60 
MQISDTGRSH TPDFHAVLAR EDWHNQTITH LNRLPAHPVF ASWRDELAAR DNLPSSRRRQ 

        70         80         90        100        110        120 
LDGSGSSLTP AARLPSMRVV TQDLPDCRGT PVPSNWQMEG YDAPIYTNVR YPIDTTPPRV 

       130        140        150        160        170        180 
PEDNPTGCYS LHFTVEDTWR ENGQTQIIFD GVNSAFHLWC NGVWVGYSQD SRLPAAFDLS 

       190        200        210        220        230        240 
PFLRPGDNRL CVMVMRWSAG SWLEDQDMWR MSGIFRSVWL LNKPQQRLCD VQLTPALDAL 

       250        260        270        280        290        300 
YRDGTLQVQA TIEATEAALA GLSVGVSLWR GEEQFAAGRQ PLGTPTVDER GHYAERVDFS 

       310        320        330        340        350        360 
LAVATPAHWS AETPNCYRAV VTLWRGDELL EAEAWDIGFR RIEIADGLLR LNGKPLLIRG 

       370        380        390        400        410        420 
VNRHEHHHLR GQVVTEADMV QDILLMKQNN FNAVRCSHYP NAPRWYELCN RYGLYVVDEA 

       430        440        450        460        470        480 
NIETHGMVPM NRLSDDPAWL PAFSARVTRM VQSNRNHPCI IIWSLGNESG GGGNHEALYH 

       490        500        510        520        530        540 
WLKRNDPSRP VQYEGGGADT TATDIICPMY ARVERDQPIP AVPKWGIKKW ISLPGEQRPL 

       550        560        570        580        590        600 
ILCEYAHAMG NSLGNFADYW QAFREYPRLQ GGFIWDWADQ AIRKTFADGS VGWAYGGDFG 

       610        620        630        640        650        660 
DKPNDRQFCM NGLVFPDRTP HPSLVEAKHA QQYFQFTLLS TSPLRVRIIS EYLFRPTDNE 

       670        680        690        700        710        720 
VVRWQVQAAG EPLYHGDLTL ALPPEGSDEI TLLDSLILPE GARAVWLTLE VTQPQATAWS 

       730        740        750        760        770        780 
EAEHRVAWQQ FPLPAPLGCR RPPCLPALPD LIVSDEVWQI RAGSQCWTID RRTGLLSRWS 

       790        800        810        820        830        840 
VGGQEQLLTP LRDQFIRAPL DNDIGVSEVE RIDPNAWVER WRSAGLYDLE AHCVQCDAQR 

       850        860        870        880        890        900 
LANETLVDCR WHYLRGEEVV IVSHWRMHFT ADGTLRLAVD GERAETLPPL PRVGLHFQVA 

       910        920        930        940        950        960 
DQQAPVSWLG LGPHENYPDR RSSACFARWE QPLAAMTTPY IFPTENGLRC DTQALDWGRW 

       970        980        990       1000       1010       1020 
HISGHFHFSV QPWSTRQLME TDHWHKMQAE DGVWITLDGL HMGVGGDDSW TPSVLPQWLL 

      1030 
SQTRWQYEVS LRSL 

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References

[1]"Nucleotide sequence of Klebsiella pneumoniae lac genes."
Buvinger W.E., Riley M.
J. Bacteriol. 163:850-857(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11441 Genomic DNA. Translation: AAA25082.1.
PIRA24925.

3D structure databases

ProteinModelPortalP06219.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL1_KLEPN
AccessionPrimary (citable) accession number: P06219
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries