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Protein

Beta-galactosidase

Gene

lacZ

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei108SubstrateUniRule annotation1
Metal bindingi207SodiumUniRule annotation1
Binding sitei207SubstrateUniRule annotation1
Sitei364Transition state stabilizerUniRule annotation1
Sitei398Transition state stabilizerUniRule annotation1
Metal bindingi423Magnesium 1UniRule annotation1
Metal bindingi425Magnesium 1UniRule annotation1
Active sitei468Proton donorUniRule annotation1
Metal bindingi468Magnesium 1UniRule annotation1
Binding sitei468SubstrateUniRule annotation1
Active sitei544NucleophileUniRule annotation1
Metal bindingi604Magnesium 2UniRule annotation1
Metal bindingi608Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi611SodiumUniRule annotation1
Binding sitei611SubstrateUniRule annotation1
Binding sitei1010SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000576621 – 1034Beta-galactosidaseAdd BLAST1034

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi272620.KPN_01610.

Structurei

3D structure databases

ProteinModelPortaliP06219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni544 – 547Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06219-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQISDTGRSH TPDFHAVLAR EDWHNQTITH LNRLPAHPVF ASWRDELAAR
60 70 80 90 100
DNLPSSRRRQ LDGSGSSLTP AARLPSMRVV TQDLPDCRGT PVPSNWQMEG
110 120 130 140 150
YDAPIYTNVR YPIDTTPPRV PEDNPTGCYS LHFTVEDTWR ENGQTQIIFD
160 170 180 190 200
GVNSAFHLWC NGVWVGYSQD SRLPAAFDLS PFLRPGDNRL CVMVMRWSAG
210 220 230 240 250
SWLEDQDMWR MSGIFRSVWL LNKPQQRLCD VQLTPALDAL YRDGTLQVQA
260 270 280 290 300
TIEATEAALA GLSVGVSLWR GEEQFAAGRQ PLGTPTVDER GHYAERVDFS
310 320 330 340 350
LAVATPAHWS AETPNCYRAV VTLWRGDELL EAEAWDIGFR RIEIADGLLR
360 370 380 390 400
LNGKPLLIRG VNRHEHHHLR GQVVTEADMV QDILLMKQNN FNAVRCSHYP
410 420 430 440 450
NAPRWYELCN RYGLYVVDEA NIETHGMVPM NRLSDDPAWL PAFSARVTRM
460 470 480 490 500
VQSNRNHPCI IIWSLGNESG GGGNHEALYH WLKRNDPSRP VQYEGGGADT
510 520 530 540 550
TATDIICPMY ARVERDQPIP AVPKWGIKKW ISLPGEQRPL ILCEYAHAMG
560 570 580 590 600
NSLGNFADYW QAFREYPRLQ GGFIWDWADQ AIRKTFADGS VGWAYGGDFG
610 620 630 640 650
DKPNDRQFCM NGLVFPDRTP HPSLVEAKHA QQYFQFTLLS TSPLRVRIIS
660 670 680 690 700
EYLFRPTDNE VVRWQVQAAG EPLYHGDLTL ALPPEGSDEI TLLDSLILPE
710 720 730 740 750
GARAVWLTLE VTQPQATAWS EAEHRVAWQQ FPLPAPLGCR RPPCLPALPD
760 770 780 790 800
LIVSDEVWQI RAGSQCWTID RRTGLLSRWS VGGQEQLLTP LRDQFIRAPL
810 820 830 840 850
DNDIGVSEVE RIDPNAWVER WRSAGLYDLE AHCVQCDAQR LANETLVDCR
860 870 880 890 900
WHYLRGEEVV IVSHWRMHFT ADGTLRLAVD GERAETLPPL PRVGLHFQVA
910 920 930 940 950
DQQAPVSWLG LGPHENYPDR RSSACFARWE QPLAAMTTPY IFPTENGLRC
960 970 980 990 1000
DTQALDWGRW HISGHFHFSV QPWSTRQLME TDHWHKMQAE DGVWITLDGL
1010 1020 1030
HMGVGGDDSW TPSVLPQWLL SQTRWQYEVS LRSL
Length:1,034
Mass (Da):117,517
Last modified:January 1, 1988 - v1
Checksum:i8F8B9DC7521EF649
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11441 Genomic DNA. Translation: AAA25082.1.
PIRiA24925.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11441 Genomic DNA. Translation: AAA25082.1.
PIRiA24925.

3D structure databases

ProteinModelPortaliP06219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_01610.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL1_KLEPN
AccessioniPrimary (citable) accession number: P06219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.