Reviewed, UniProtKB/Swiss-Prot P06214 (HEM2_RAT)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:

Alad

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Zinc.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from direct assay. Source: RGD response to arsenic Inferred from direct assay. Source: RGD response to cadmium ion Inferred from direct assay. Source: RGD response to drug Inferred from direct assay. Source: RGD response to ethanol Inferred from direct assay. Source: RGD response to lead ion Inferred from expression pattern. Source: RGD response to nutrient Inferred from direct assay. Source: RGD response to zinc ion Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD extracellular space Inferred from direct assay. Source: RGD
Molecular function	porphobilinogen synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Delta-aminolevulinic acid dehydratase		PRO_0000140530
Sites
	Active site	252	1	By similarity
	Metal binding	122	1	Zinc; catalytic By similarity
	Metal binding	124	1	Zinc; catalytic By similarity
	Metal binding	132	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06214-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 617F6E85B647AB25	FASTA	330	36,032
	10 20 30 40 50 60 MHHQSVLHSG YFHPLLRAWQ TTPSTVSATN LIYPIFVTDV PDDVQPIASL PGVARYGVNQ 70 80 90 100 110 120 LEEMLRPLVE AGLRCVLIFG VPSRVPKDEQ GSAADSEDSP TIEAVRLLRK TFPTLLVACD 130 140 150 160 170 180 VCLCPYTSHG HCGLLSENGA FLAEESRQRL AEVALAYAKA GCQVVAPSDM MDGRVEAIKA 190 200 210 220 230 240 ALLKHGLGNR VSVMSYSAKF ASCFYGPFRD AAQSSPAFGD RRCYQLPPGA RGLALRAVAR 250 260 270 280 290 300 DIQEGADILM VKPGLPYLDM VQEVKDKHPE LPLAVYQVSG EFAMLWHGAK AGAFDLRTAV 310 320 330 LESMTAFRRA GADIIITYFA PQLLKWLKEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of rat liver delta-aminolevulinic acid dehydratase cDNA." Bishop T.R., Frelin L.P., Boyer S.H. Nucleic Acids Res. 14:10115-10115(1986) [PubMed: 3808948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[3]	"Isolation of a rat liver delta-aminolevulinate dehydrase (ALAD) cDNA clone: evidence for unequal ALAD gene dosage among inbred mouse strains." Bishop T.R., Cohen P.J., Boyer S.H., Noyes A.N., Frelin L.P. Proc. Natl. Acad. Sci. U.S.A. 83:5568-5572(1986) [PubMed: 3502704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-45.
[4]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 56-84 AND 200-209, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04959 mRNA. Translation: CAA28621.1. BC061806 mRNA. Translation: AAH61806.1.
IPI	IPI00201399.
PIR	A24724.
RefSeq	NP_037031.1.
UniGene	Rn.3941
3D structure databases
HSSP	HSSP built from PDB template 1E51 based on UniProtKB P13716.
SMR	P06214. Positions 1-328.
ModBase	Search...
PTM databases
PhosphoSite	P06214.
Proteomic databases
PRIDE	P06214.
Genome annotation databases
Ensembl	ENSRNOG00000015206. Rattus norvegicus. [Contig view]
GeneID	25374.
KEGG	rno:25374.
NMPDR	fig|10116.3.peg.23271.
Organism-specific databases
RGD	2083. Alad.
Phylogenomic databases
HOVERGEN	P06214.
OMA	P06214. PELGVIT.
Enzyme and pathway databases
BRENDA	4.2.1.24. 248.
Gene expression databases
GermOnline	ENSRNOG00000015206. Rattus norvegicus.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
ProDom	PD002304. AlaD_dehydratase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606397.

Entry information

Entry name

HEM2_RAT

Accession

Primary (citable) accession number: P06214

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)