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Protein

Muscarinic acetylcholine receptor M2

Gene

CHRM2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M2
Gene namesi
Name:CHRM2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22ExtracellularBy similarityAdd BLAST22
Transmembranei23 – 45Helical; Name=1By similarityAdd BLAST23
Topological domaini46 – 59CytoplasmicBy similarityAdd BLAST14
Transmembranei60 – 80Helical; Name=2By similarityAdd BLAST21
Topological domaini81 – 97ExtracellularBy similarityAdd BLAST17
Transmembranei98 – 119Helical; Name=3By similarityAdd BLAST22
Topological domaini120 – 139CytoplasmicBy similarityAdd BLAST20
Transmembranei140 – 162Helical; Name=4By similarityAdd BLAST23
Topological domaini163 – 184ExtracellularBy similarityAdd BLAST22
Transmembranei185 – 209Helical; Name=5By similarityAdd BLAST25
Topological domaini210 – 387CytoplasmicBy similarityAdd BLAST178
Transmembranei388 – 410Helical; Name=6By similarityAdd BLAST23
Topological domaini411 – 418ExtracellularBy similarity8
Transmembranei419 – 442Helical; Name=7By similarityAdd BLAST24
Topological domaini443 – 466CytoplasmicBy similarityAdd BLAST24

GO - Cellular componenti

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4781.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690241 – 466Muscarinic acetylcholine receptor M2Add BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi3N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi6N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi96 ↔ 176PROSITE-ProRule annotation
Modified residuei232PhosphoserineBy similarity1
Disulfide bondi413 ↔ 416PROSITE-ProRule annotation
Modified residuei446PhosphothreonineSequence analysis1
Modified residuei450PhosphothreonineSequence analysis1
Modified residuei465PhosphothreonineSequence analysis1

Post-translational modificationi

Phosphorylated in response to agonist treatment.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP06199.

Interactioni

Subunit structurei

Interacts with ARRB1 and ARRB2 (By similarity). Interacts with RACK1; the interaction regulates CHRM2 internalization.By similarity1 Publication

Protein-protein interaction databases

IntActiP06199. 1 interactor.

Chemistry databases

BindingDBiP06199.

Structurei

3D structure databases

ProteinModelPortaliP06199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 107Agonist bindingBy similarity5
Regioni403 – 404Agonist bindingBy similarity2
Regioni426 – 430Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi120 – 122Important for signaling3
Motifi436 – 440Important for signaling5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG105720.
InParanoidiP06199.
KOiK04130.

Family and domain databases

CDDicd15297. 7tmA_mAChR_M2. 1 hit.
InterProiView protein in InterPro
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
PANTHERiPTHR24249:SF359. PTHR24249:SF359. 1 hit.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P06199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSTNSSNS GLALTSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
60 70 80 90 100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
110 120 130 140 150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
160 170 180 190 200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
210 220 230 240 250
IMTVLYWHIS RASKSRIKKD KKEPVANQEP VSPSLVQGRI VKPNNNNMPG
260 270 280 290 300
SDEALEHNKI QNGKAPRDAV TENCVQGEEK ESSNDSTSVS AVASNMRDDE
310 320 330 340 350
ITQDENTVST SLGHSKDENS KQTCIKIVTK TQKSDSCTPA NTTVELVGSS
360 370 380 390 400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
410 420 430 440 450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
460
FKHLLMCHYK NIGATR
Length:466
Mass (Da):51,673
Last modified:January 1, 1988 - v1
Checksum:i53D089F179789CD9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti330K → N in AAA30986 (PubMed:3107123).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04708 mRNA. Translation: CAA28413.1.
M16331 Genomic DNA. Translation: AAA30986.1.
PIRiA27386.
RefSeqiNP_999426.1. NM_214261.1.
UniGeneiSsc.16247.

Genome annotation databases

GeneIDi397498.
KEGGissc:397498.

Similar proteinsi

Entry informationi

Entry nameiACM2_PIG
AccessioniPrimary (citable) accession number: P06199
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: August 30, 2017
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families