Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Muscarinic acetylcholine receptor M2

Gene

CHRM2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M2
Gene namesi
Name:CHRM2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222ExtracellularBy similarityAdd
BLAST
Transmembranei23 – 4523Helical; Name=1By similarityAdd
BLAST
Topological domaini46 – 5914CytoplasmicBy similarityAdd
BLAST
Transmembranei60 – 8021Helical; Name=2By similarityAdd
BLAST
Topological domaini81 – 9717ExtracellularBy similarityAdd
BLAST
Transmembranei98 – 11922Helical; Name=3By similarityAdd
BLAST
Topological domaini120 – 13920CytoplasmicBy similarityAdd
BLAST
Transmembranei140 – 16223Helical; Name=4By similarityAdd
BLAST
Topological domaini163 – 18422ExtracellularBy similarityAdd
BLAST
Transmembranei185 – 20925Helical; Name=5By similarityAdd
BLAST
Topological domaini210 – 387178CytoplasmicBy similarityAdd
BLAST
Transmembranei388 – 41023Helical; Name=6By similarityAdd
BLAST
Topological domaini411 – 4188ExtracellularBy similarity
Transmembranei419 – 44224Helical; Name=7By similarityAdd
BLAST
Topological domaini443 – 46624CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4781.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Muscarinic acetylcholine receptor M2PRO_0000069024Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence analysis
Glycosylationi3 – 31N-linked (GlcNAc...)Sequence analysis
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence analysis
Disulfide bondi96 ↔ 176PROSITE-ProRule annotation
Modified residuei232 – 2321PhosphoserineBy similarity
Disulfide bondi413 ↔ 416PROSITE-ProRule annotation
Modified residuei446 – 4461PhosphothreonineSequence analysis
Modified residuei450 – 4501PhosphothreonineSequence analysis
Modified residuei465 – 4651PhosphothreonineSequence analysis

Post-translational modificationi

Phosphorylated in response to agonist treatment.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Interacts with ARRB1 and ARRB2 (By similarity). Interacts with RACK1; the interaction regulates CHRM2 internalization.By similarity1 Publication

Protein-protein interaction databases

IntActiP06199. 1 interaction.

Chemistry

BindingDBiP06199.

Structurei

3D structure databases

ProteinModelPortaliP06199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni103 – 1075Agonist bindingBy similarity
Regioni403 – 4042Agonist bindingBy similarity
Regioni426 – 4305Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi120 – 1223Important for signaling
Motifi436 – 4405Important for signaling

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG105720.
InParanoidiP06199.
KOiK04130.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF57. PTHR24249:SF57. 4 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSTNSSNS GLALTSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
60 70 80 90 100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
110 120 130 140 150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
160 170 180 190 200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
210 220 230 240 250
IMTVLYWHIS RASKSRIKKD KKEPVANQEP VSPSLVQGRI VKPNNNNMPG
260 270 280 290 300
SDEALEHNKI QNGKAPRDAV TENCVQGEEK ESSNDSTSVS AVASNMRDDE
310 320 330 340 350
ITQDENTVST SLGHSKDENS KQTCIKIVTK TQKSDSCTPA NTTVELVGSS
360 370 380 390 400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
410 420 430 440 450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
460
FKHLLMCHYK NIGATR
Length:466
Mass (Da):51,673
Last modified:January 1, 1988 - v1
Checksum:i53D089F179789CD9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti330 – 3301K → N in AAA30986 (PubMed:3107123).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04708 mRNA. Translation: CAA28413.1.
M16331 Genomic DNA. Translation: AAA30986.1.
PIRiA27386.
RefSeqiNP_999426.1. NM_214261.1.
UniGeneiSsc.16247.

Genome annotation databases

GeneIDi397498.
KEGGissc:397498.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04708 mRNA. Translation: CAA28413.1.
M16331 Genomic DNA. Translation: AAA30986.1.
PIRiA27386.
RefSeqiNP_999426.1. NM_214261.1.
UniGeneiSsc.16247.

3D structure databases

ProteinModelPortaliP06199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP06199. 1 interaction.

Chemistry

BindingDBiP06199.
ChEMBLiCHEMBL4781.

Protein family/group databases

GPCRDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397498.
KEGGissc:397498.

Organism-specific databases

CTDi1129.

Phylogenomic databases

HOVERGENiHBG105720.
InParanoidiP06199.
KOiK04130.

Miscellaneous databases

PROiP06199.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF57. PTHR24249:SF57. 4 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM2_PIG
AccessioniPrimary (citable) accession number: P06199
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.