P06199 (ACM2_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Muscarinic acetylcholine receptor M2

Gene names

Name:

CHRM2

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Subunit structure	Interacts with ARRB1 and ARRB2. Interacts with GNB2L1/RACK1; the interaction regulates CHRM2 internalization By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled acetylcholine receptor signaling pathway Inferred from electronic annotation. Source: GOC
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled acetylcholine receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Muscarinic acetylcholine receptor M2		PRO_0000069024
Regions
	Topological domain	1 – 22	22	Extracellular By similarity
	Transmembrane	23 – 45	23	Helical; Name=1; By similarity
	Topological domain	46 – 59	14	Cytoplasmic By similarity
	Transmembrane	60 – 80	21	Helical; Name=2; By similarity
	Topological domain	81 – 97	17	Extracellular By similarity
	Transmembrane	98 – 119	22	Helical; Name=3; By similarity
	Topological domain	120 – 139	20	Cytoplasmic By similarity
	Transmembrane	140 – 162	23	Helical; Name=4; By similarity
	Topological domain	163 – 184	22	Extracellular By similarity
	Transmembrane	185 – 207	23	Helical; Name=5; By similarity
	Topological domain	208 – 388	181	Cytoplasmic By similarity
	Transmembrane	389 – 409	21	Helical; Name=6; By similarity
	Topological domain	410 – 423	14	Extracellular By similarity
	Transmembrane	424 – 443	20	Helical; Name=7; By similarity
	Topological domain	444 – 466	23	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	232	1	Phosphoserine By similarity
	Modified residue	234	1	Phosphoserine By similarity
	Modified residue	446	1	Phosphothreonine Potential
	Modified residue	450	1	Phosphothreonine Potential
	Modified residue	465	1	Phosphothreonine Potential
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	6	1	N-linked (GlcNAc...) Potential
	Disulfide bond	96 ↔ 176		By similarity
Experimental info
	Sequence conflict	330	1	K → N in AAA30986. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P06199 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 53D089F179789CD9	FASTA	466	51,673
	10 20 30 40 50 60 MNNSTNSSNS GLALTSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV NRHLQTVNNY 70 80 90 100 110 120 FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL ALDYVVSNAS VMNLLIISFD 130 140 150 160 170 180 RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL SFILWAPAIL FWQFIVGVRT VEDGECYIQF 190 200 210 220 230 240 FSNAAVTFGT AIAAFYLPVI IMTVLYWHIS RASKSRIKKD KKEPVANQEP VSPSLVQGRI 250 260 270 280 290 300 VKPNNNNMPG SDEALEHNKI QNGKAPRDAV TENCVQGEEK ESSNDSTSVS AVASNMRDDE 310 320 330 340 350 360 ITQDENTVST SLGHSKDENS KQTCIKIVTK TQKSDSCTPA NTTVELVGSS GQNGDEKQNI 370 380 390 400 410 420 VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW APYNVMVLIN TFCAPCIPNT 430 440 450 460 VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR

References

[1]	"Primary structure of porcine cardiac muscarinic acetylcholine receptor deduced from the cDNA sequence." Kubo T., Maeda A., Sugimoto K., Akiba I., Mikami A., Takahashi H., Haga T., Haga K., Ichiyama A., Kangawa K., Matsuo H., Hirose T., Numa S. FEBS Lett. 209:367-372(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Primary structure and biochemical properties of an M2 muscarinic receptor." Peralta E.G., Winslow J.W., Peterson G.L., Smith D.H., Ashkenazi A., Ramachandran J., Schimerlik M.I., Capon D.J. Science 236:600-606(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04708 mRNA. Translation: CAA28413.1. M16331 Genomic DNA. Translation: AAA30986.1.
PIR	A27386.
RefSeq	NP_999426.1. NM_214261.1.
UniGene	Ssc.16247.
3D structure databases
ProteinModelPortal	P06199.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06199. 1 interaction.
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	397498.
KEGG	ssc:397498.
Organism-specific databases
CTD	1129.
Phylogenomic databases
HOVERGEN	HBG105720.
KO	K04130.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001065. Musac_M2_rcpt. IPR000995. Musac_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00539. MUSCRINICM2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P06199.
ChEMBL	CHEMBL4781.

Entry information

Entry name

ACM2_PIG

Accession

Primary (citable) accession number: P06199

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program