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Protein

CDP-diacylglycerol--inositol 3-phosphatidyltransferase

Gene

PIS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol.

GO - Molecular functioni

  • CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity Source: SGD

GO - Biological processi

  • phosphatidylinositol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15158.
YEAST:YPR113W-MONOMER.
ReactomeiR-SCE-1483226. Synthesis of PI.

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase (EC:2.7.8.11)
Alternative name(s):
Phosphatidylinositol synthase
Short name:
PI synthase
Short name:
PtdIns synthase
Gene namesi
Name:PIS1
Synonyms:PIS
Ordered Locus Names:YPR113W
ORF Names:P8283.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR113W.
SGDiS000006317. PIS1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicSequence analysisAdd
BLAST
Transmembranei21 – 4121HelicalSequence analysisAdd
BLAST
Topological domaini42 – 454LumenalSequence analysis
Transmembranei46 – 6621HelicalSequence analysisAdd
BLAST
Topological domaini67 – 759CytoplasmicSequence analysis
Transmembranei76 – 9621HelicalSequence analysisAdd
BLAST
Topological domaini97 – 982LumenalSequence analysis
Transmembranei99 – 11921HelicalSequence analysisAdd
BLAST
Topological domaini120 – 14526CytoplasmicSequence analysisAdd
BLAST
Transmembranei146 – 16621HelicalSequence analysisAdd
BLAST
Topological domaini167 – 1704LumenalSequence analysis
Transmembranei171 – 19121HelicalSequence analysisAdd
BLAST
Topological domaini192 – 22029CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: SGD
  • integral component of membrane Source: SGD
  • mitochondrial outer membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220CDP-diacylglycerol--inositol 3-phosphatidyltransferasePRO_0000056807Add
BLAST

Proteomic databases

MaxQBiP06197.

Interactioni

Protein-protein interaction databases

BioGridi36280. 50 interactions.
DIPiDIP-3815N.
IntActiP06197. 33 interactions.
MINTiMINT-541641.

Structurei

3D structure databases

ProteinModelPortaliP06197.
SMRiP06197. Positions 19-97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017279.
HOGENOMiHOG000204051.
InParanoidiP06197.
KOiK00999.
OMAiVAWVGYY.
OrthoDBiEOG092C5EEC.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014387. CDP_diag_ino_3_P_euk.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000848. CDP_diag_ino_3_P. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNSTPEKV TAEHVLWYIP NKIGYVRVIT AALSFFVMKN HPTAFTWLYS
60 70 80 90 100
TSCLLDALDG TMARKYNQVS SLGAVLDMVT DRSSTAGLMC FLCVQYPQWC
110 120 130 140 150
VFFQLMLGLD ITSHYMHMYA SLSAGKTSHK SVGEGESRLL HLYYTRRDVL
160 170 180 190 200
FTICAFNELF YAGLYLQLFS NSATFGKWTT IISFPGYVFK QTANVVQLKR
210 220
AALILADNDA KNANEKNKTY
Length:220
Mass (Da):24,824
Last modified:January 1, 1988 - v1
Checksum:i6E2E62252B3CB5E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02697 Genomic DNA. Translation: AAA34876.1.
U32445 Genomic DNA. Translation: AAB68083.1.
BK006949 Genomic DNA. Translation: DAA11528.1.
PIRiA27409.
RefSeqiNP_015438.1. NM_001184210.1.

Genome annotation databases

EnsemblFungiiYPR113W; YPR113W; YPR113W.
GeneIDi856229.
KEGGisce:YPR113W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02697 Genomic DNA. Translation: AAA34876.1.
U32445 Genomic DNA. Translation: AAB68083.1.
BK006949 Genomic DNA. Translation: DAA11528.1.
PIRiA27409.
RefSeqiNP_015438.1. NM_001184210.1.

3D structure databases

ProteinModelPortaliP06197.
SMRiP06197. Positions 19-97.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36280. 50 interactions.
DIPiDIP-3815N.
IntActiP06197. 33 interactions.
MINTiMINT-541641.

Proteomic databases

MaxQBiP06197.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR113W; YPR113W; YPR113W.
GeneIDi856229.
KEGGisce:YPR113W.

Organism-specific databases

EuPathDBiFungiDB:YPR113W.
SGDiS000006317. PIS1.

Phylogenomic databases

GeneTreeiENSGT00390000017279.
HOGENOMiHOG000204051.
InParanoidiP06197.
KOiK00999.
OMAiVAWVGYY.
OrthoDBiEOG092C5EEC.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15158.
YEAST:YPR113W-MONOMER.
ReactomeiR-SCE-1483226. Synthesis of PI.

Miscellaneous databases

PROiP06197.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014387. CDP_diag_ino_3_P_euk.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000848. CDP_diag_ino_3_P. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIS_YEAST
AccessioniPrimary (citable) accession number: P06197
Secondary accession number(s): D6W4B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Myo-inositol-containing phospholipids are of crucial importance in the control of cellular functions.
Present with 3810 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.