P06185 (PGTE_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer membrane protease E
Short name=E protein
EC=3.4.23.-

Gene names

Name:	pgtE
Synonyms:	prtA
Ordered Locus Names:	STM2395

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protease that can cleave T7 RNA polymerase. Specific for paired basic residues. Ref.4
Subcellular location	Cell outer membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase A26 family.
Sequence caution	The sequence AAA27185.1 differs from that shown. Reason: Frameshift at positions 7, 278, 288 and 295.

Ontologies

Keywords
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal Transmembrane Transmembrane beta strand
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37	Potential
	Chain	38 – 312	275	Outer membrane protease E		PRO_0000025818
Sites
	Active site	104	1	By similarity
	Active site	106	1	By similarity
	Active site	226	1	By similarity
	Active site	228	1	By similarity
Experimental info
	Sequence conflict	296	1	G → S Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P06185 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 5A6841732AF2E8C4	FASTA	312	35,055
	10 20 30 40 50 60 MKKHAIAVMM IAVFSESVYA ESALFIPDVS PDSVTTSLSV GVLNGKSREL VYDTDTGRKL 70 80 90 100 110 120 SQLDWKIKNV ATLQGDLSWE PYSFMTLDAR GWTSLASGSG HMVDHDWMSS EQPGWTDRSI 130 140 150 160 170 180 HPDTSVNYAN EYDLNVKGWL LQGDNYKAGV TAGYQETRFS WTARGGSYIY DNGRYIGNFP 190 200 210 220 230 240 HGVRGIGYSQ RFEMPYIGLA GDYRINDFEC NVLFKYSDWV NAHDNDEHYM RKLTFREKTE 250 260 270 280 290 300 NSRYYGASID AGYYITSNAK IFAEFAYSKY EEGKGGTQII DKTSGDTAYF GGDAAGIANN 310 NYTVTAGLQY RF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and nucleotide sequence of the activator gene of the externally induced phosphoglycerate transport system of Salmonella typhimurium." Yu G.-Q., Hong J.-S. Gene 45:51-57(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A PhoP-regulated outer membrane protease of Salmonella enterica serovar typhimurium promotes resistance to alpha-helical antimicrobial peptides." Guina T., Yi E.C., Wang H., Hackett M., Miller S.I. J. Bacteriol. 182:4077-4086(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[4]	"Comparison of Escherichia coli K-12 outer membrane protease OmpT and Salmonella typhimurium E protein." Grodberg J., Dunn J.J. J. Bacteriol. 171:2903-2905(1989) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO OMPT, FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M13923 Genomic DNA. Translation: AAA27185.1. Frameshift. AF239770 Genomic DNA. Translation: AAF85951.1. AE006468 Genomic DNA. Translation: AAL21295.1.
PIR	B28255.
RefSeq	NP_461336.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P06185.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM2395.
Protein family/group databases
MEROPS	A26.004.
Proteomic databases
PaxDb	P06185.
PRIDE	P06185.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL21295; AAL21295; STM2395.
GeneID	1253917.
KEGG	stm:STM2395.
PATRIC	32383409. VBISalEnt20916_2533.
Phylogenomic databases
eggNOG	COG4571.
HOGENOM	HOG000117799.
KO	K08477.
OMA	THWSHHE.
ProtClustDB	PRK10993.
Family and domain databases
Gene3D	2.40.128.90. 1 hit.
InterPro	IPR020080. OM_adhesin/peptidase_omptin. IPR023619. Peptidase_A26. IPR020079. Peptidase_A26_CS. IPR000036. Peptidase_A26_omptin. [Graphical view]
Pfam	PF01278. Omptin. 1 hit. [Graphical view]
PIRSF	PIRSF001522. Peptidase_A26. 1 hit.
PRINTS	PR00482. OMPTIN.
SUPFAM	SSF69917. OM_adhesin/protease_omptin. 1 hit.
PROSITE	PS00834. OMPTIN_1. 1 hit. PS00835. OMPTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGTE_SALTY

Accession

Primary (citable) accession number: P06185
Secondary accession number(s): Q9KI72

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program