P06182 (CCHL_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c heme lyase
Short name=CCHL
EC=4.4.1.17

Alternative name(s):
Holocytochrome-c synthase

Gene names

Name:	CYC3
Ordered Locus Names:	YAL039C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Links covalently the heme group to the apoprotein of cytochrome c.
Catalytic activity	Holocytochrome c = apocytochrome c + heme.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 3870 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the cytochrome c-type heme lyase family. Contains 2 HRM (heme regulatory motif) repeats.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat
Ligand	Heme Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytochrome c-heme linkage Inferred from direct assay Ref.1. Source: SGD
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial intermembrane space Inferred from direct assay. Source: SGD
Molecular function	holocytochrome-c synthase activity Inferred from direct assay Ref.1. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YHR080C	P38800	1	EBI-4378,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Cytochrome c heme lyase		PRO_0000121718
Regions
	Repeat	25 – 30	6	HRM 1
	Repeat	41 – 46	6	HRM 2

Sequences

	Sequence		Length	Mass (Da)
	P06182 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: A672A48BBFD848AF	FASTA	269	30,081
	10 20 30 40 50 60 MGWFWADQKT TGKDIGGAAV SSMSGCPVMH ESSSSSPPSS ECPVMQGDND RINPLNNMPE 70 80 90 100 110 120 LAASKQPGQK MDLPVDRTIS SIPKSPDSNE FWEYPSPQQM YNAMVRKGKI GGSGEVAEDA 130 140 150 160 170 180 VESMVQVHNF LNEGCWQEVL EWEKPHTDES HVQPKLLKFM GKPGVLSPRA RWMHLCGLLF 190 200 210 220 230 240 PSHFSQELPF DRHDWIVLRG ERKAEQQPPT FKEVRYVLDF YGGPDDENGM PTFHVDVRPA 250 260 LDSLDNAKDR MTRFLDRMIS GPSSSSSAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and sequence of the gene encoding cytochrome c heme lyase in the yeast Saccharomyces cerevisiae." Dumont M.E., Ernst J.F., Hampsey D.M., Sherman F. EMBO J. 6:235-241(1987) [PubMed: 3034577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B-7034.
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04776 Genomic DNA. Translation: CAA28470.1. U12980 Genomic DNA. Translation: AAC04992.1. BK006935 Genomic DNA. Translation: DAA06947.1.
PIR	A26162.
RefSeq	NP_009361.1. NM_001178184.1.
3D structure databases
ProteinModelPortal	P06182.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06182. 14 interactions.
STRING	P06182.
Proteomic databases
PeptideAtlas	P06182.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL039C; YAL039C; YAL039C.
GeneID	851192.
KEGG	sce:YAL039C.
NMPDR	fig|4932.3.peg.38.
Organism-specific databases
CYGD	YAL039c.
SGD	S000000037. CYC3.
Phylogenomic databases
eggNOG	fuNOG07296.
GeneTree	EFGT00050000005300.
HOGENOM	HBG627179.
OMA	IDYYSAP.
OrthoDB	EOG4MSH77.
Gene expression databases
ArrayExpress	P06182.
Genevestigator	P06182.
GermOnline	YAL039C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000511. Cyt_C/C1_haem_lyase. [Graphical view]
KO	K01764.
PANTHER	PTHR12743. Cyto_heme_lyase. 1 hit.
Pfam	PF01265. Cyto_heme_lyase. 1 hit. [Graphical view]
PROSITE	PS00821. CYTO_HEME_LYASE_1. 1 hit. PS00822. CYTO_HEME_LYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968034.

Entry information

Entry name

CCHL_YEAST

Accession

Primary (citable) accession number: P06182
Secondary accession number(s): D6VPH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program