P06179 (FLIC_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flagellin

Alternative name(s):
Phase 1-I flagellin

Gene names

Name:	fliC
Synonyms:	flaF, hag
Ordered Locus Names:	STM1959

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
Subcellular location	Secreted. Bacterial flagellum.
Induction	Inhibited in nutrient-poor medium (at protein level). Ref.10
Miscellaneous	Individual Salmonella serotypes usually alternate between the production of 2 antigenic forms of flagella, termed phase 1 and phase 2, each specified by separate structural genes, fliC and fljB.
Sequence similarities	Belongs to the bacterial flagellin family.

Ontologies

Keywords
Cellular component	Bacterial flagellum Secreted
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MyD88-dependent toll-like receptor signaling pathway Traceable author statement. Source: Reactome ciliary or flagellar motility Inferred from electronic annotation. Source: InterPro toll-like receptor 15 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 2 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 4 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 5 signaling pathway Traceable author statement. Source: Reactome
Cellular_component	bacterial-type flagellum filament Inferred from electronic annotation. Source: InterPro extracellular region Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliS	P26609	4	EBI-2011501,EBI-2011519

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 495

494

Flagellin

PRO_0000182578

Experimental info

Sequence conflict

127

1

S → N in AAA27072. Ref.1

Sequence conflict

133

1

N → S in AAA27072. Ref.1

Sequence conflict

215

1

Q → E in AAA27072. Ref.1

Sequence conflict

261 – 273

13

GGATS…GGLPA → AVTPATVT in AAA27072. Ref.1

Sequence conflict

277 – 284

8

EDVKNVQV → ALSGKMYS in AAA27072. Ref.1

Sequence conflict

287 – 291

5

ADLTE → PDSDI in AAA27072. Ref.1

Sequence conflict

338

1

N → D in AAA27072. Ref.1

Sequence conflict

346

1

N → D in AAA27072. Ref.1

Sequence conflict

354

1

D → N in AAA27072. Ref.1

Sequence conflict

375 – 377

3

SIG → TID in AAA27072. Ref.1

Sequence conflict

382

1

A → N in AAA27072. Ref.1

Sequence conflict

387

1

E → A in AAA27072. Ref.1

Sequence conflict

390

1

N → D in AAA27072. Ref.1

Sequence conflict

394 – 396

3

QPD → EPE in AAA27072. Ref.1

Sequence conflict

400

1

A → Q in AAA27072. Ref.1

Sequence conflict

403

1

T → K in AAA27072. Ref.1

Sequence conflict

448

1

T → S in AAA27072. Ref.1

Secondary structure

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495

Helix Strand Turn

Details...

Helix

59 – 99

41

Helix

106 – 129

24

Turn

137 – 139

3

Beta strand

142 – 147

6

Beta strand

149 – 151

3

Beta strand

155 – 160

6

Turn

165 – 169

5

Beta strand

180 – 185

6

Beta strand

190 – 197

8

Helix

200 – 202

3

Turn

204 – 206

3

Helix

208 – 210

3

Beta strand

212 – 217

6

Beta strand

219 – 223

5

Turn

225 – 227

3

Beta strand

230 – 237

8

Beta strand

244 – 250

7

Turn

252 – 254

3

Beta strand

256 – 260

5

Beta strand

277 – 285

9

Helix

286 – 288

3

Helix

290 – 298

9

Beta strand

305 – 313

9

Beta strand

319 – 328

10

Beta strand

331 – 337

7

Beta strand

343 – 345

3

Beta strand

353 – 356

4

Beta strand

361 – 366

6

Beta strand

371 – 376

6

Beta strand

379 – 382

4

Helix

383 – 386

4

Turn

391 – 393

3

Helix

408 – 448

41

Sequences

	Sequence		Length	Mass (Da)
	P06179 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 4BD7849FA3B936BA	FASTA	495	51,612
	10 20 30 40 50 60 MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG 70 80 90 100 110 120 LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL 130 140 150 160 170 180 NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK 190 200 210 220 230 240 VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT 250 260 270 280 290 300 GKDGYYEVSV DKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAG 310 320 330 340 350 360 VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA 370 380 390 400 410 420 LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD 430 440 450 460 470 480 TLRSDLGAVQ NRFNSAITNL GNTVNNLTSA RSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL 490 AQANQVPQNV LSLLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The covalent structure of the phase-1 flagellar filament protein of Salmonella typhimurium and its comparison with other flagellins." Joys T.M. J. Biol. Chem. 260:15758-15761(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Amino acids responsible for flagellar shape are distributed in terminal regions of flagellin." Kanto S., Okino H., Aizawa S., Yamaguchi S. J. Mol. Biol. 219:471-480(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Conversion of the Salmonella phase 1 flagellin gene fliC to the phase 2 gene fljB on the Escherichia coli K-12 chromosome." Okazaki N., Matsuo S., Saito K., Tominaga A., Enomoto M. J. Bacteriol. 175:758-766(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[5]	"Flagellar hook and hook-associated proteins of Salmonella typhimurium and their relationship to other axial components of the flagellum." Homma M., Derosier D.J., Macnab R.M. J. Mol. Biol. 213:819-832(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
[6]	"Analysis of the nucleotide sequence of an invertible controlling element." Zieg J., Simon M. Proc. Natl. Acad. Sci. U.S.A. 77:4196-4200(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
[7]	"Sequence invariance of the antigen-coding central region of the phase 1 flagellar filament gene (fliC) among strains of Salmonella typhimurium." Smith N.H., Selander R.K. J. Bacteriol. 172:603-609(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 145-428.
[8]	"Point mutations that lock Salmonella typhimurium flagellar filaments in the straight right-handed and left-handed forms and their relation to filament superhelicity." Hyman H.C., Trachtenberg S. J. Mol. Biol. 220:79-88(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 411-495.
[9]	"The flagellin N-methylase gene fliB and an adjacent serovar-specific IS200 element in Salmonella typhimurium." Burnens A.P., Stanley J., Sack R., Hunziker P., Brodard I., Nicolet J. Microbiology 143:1539-1547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 476-495. Strain: LT2 / ATCC 23564.
[10]	"EAL domain protein YdiV acts as an anti-FlhD4C2 factor responsible for nutritional control of the flagellar regulon in Salmonella enterica Serovar Typhimurium." Wada T., Morizane T., Abo T., Tominaga A., Inoue-Tanaka K., Kutsukake K. J. Bacteriol. 193:1600-1611(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: LT2 / SGSC1412 / ATCC 700720.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M11332 Genomic DNA. Translation: AAA27072.1.
D13689 Genomic DNA. Translation: BAA02846.1.
AE006468 Genomic DNA. Translation: AAL20871.1.
X51740 Genomic DNA. Translation: CAA36029.1.
J01801 Genomic DNA. Translation: AAA27074.1.
M33808 Genomic DNA. Translation: AAA27080.1.
Z54217 Genomic DNA. Translation: CAA90950.1.

PIR

A24262.
S16121.

RefSeq

NP_460912.1. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IO1	X-ray	2.00	A	54-450	[»]
1P95	model	-	A	57-451	[»]
1UCU	electron microscopy	4.00	A	2-494	[»]
3A5X	electron microscopy	4.00	A	2-495	[»]

DisProt

DP00026.

ProteinModelPortal

P06179.

SMR

P06179. Positions 2-495.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-43768N.

IntAct

P06179. 1 interaction.

MINT

MINT-2831235.

STRING

99287.STM1959.

Proteomic databases

PaxDb

P06179.

PRIDE

P06179.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL20871; AAL20871; STM1959.

GeneID

1253480.

KEGG

stm:STM1959.

PATRIC

32382477. VBISalEnt20916_2074.

Phylogenomic databases

eggNOG

COG1344.

HOGENOM

HOG000255144.

KO

K02406.

OMA

IASTEWS.

ProtClustDB

PRK08026.

Enzyme and pathway databases

Reactome

REACT_147795. Innate Immune System.
REACT_6900. Immune System.
REACT_75813. Ipaf is activated.

Family and domain databases

InterPro

IPR001492. Flagellin.
IPR001029. Flagellin_D0/D1.
IPR014981. Flagellin_D3.
[Graphical view]

Pfam

PF00700. Flagellin_C. 1 hit.
PF08884. Flagellin_D3. 1 hit.
PF00669. Flagellin_N. 1 hit.
[Graphical view]

PRINTS

PR00207. FLAGELLIN.

ProtoNet

Search...

Other

EvolutionaryTrace

P06179.

Entry information

Entry name

FLIC_SALTY

Accession

Primary (citable) accession number: P06179
Secondary accession number(s): P97160, Q02871, Q56088

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 115 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program