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P06174 (HEM4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uroporphyrinogen-III synthase

Short name=UROIIIS
Short name=UROS
EC=4.2.1.75
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene names
Name:HEM4
Synonyms:ORF1
Ordered Locus Names:YOR278W
ORF Names:O5463
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III By similarity.

Catalytic activity

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the uroporphyrinogen-III synthase family.

Caution

Was originally (Ref.1) thought to be involved in mitochondrial import.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275Uroporphyrinogen-III synthase
PRO_0000135254

Experimental info

Sequence conflict231 – 27545HLRVA…YQRHK → IYGLRHRTYH in CAA28399. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P06174 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: CDC857C8CFB4E1A7

FASTA27530,912
        10         20         30         40         50         60 
MSSRKKVRVL LLKNKTVPID KYELECRSKA FEPIFVPLIK HTHVIQDFRN VLNTIPNYLN 

        70         80         90        100        110        120 
TINYIIITSQ RTVESLNEAI IPTLTSEQKA ALLSKTVYTV GPATANFIRR SGFINVKGGE 

       130        140        150        160        170        180 
DAGNGSILAD IIIDDLSTDI KACPPSELLF LVGEIRRDII PKKLHSKGIK VREVVTYKTE 

       190        200        210        220        230        240 
ELSDGFKRFI HAMKECDEDE VFSDWVVVFS PQGTKEITQY LGDSNRLPGS HLRVASIGPT 

       250        260        270 
TKKYLDDNDV TSDVVSPKPD PKSLLDAIEL YQRHK 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of a nuclear yeast gene involved in the mitochondrial import of cytoplasmically synthesized precursor proteins."
Langgut W., Entrup R., Schweizer E.
Curr. Genet. 11:177-184(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae."
Cheret G., Bernardi A., Sor F.J.
Yeast 12:1059-1064(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Isolation of the gene HEM4 encoding uroporphyrinogen III synthase in Saccharomyces cerevisiae."
Amillet J.-M., Labbe-Bois R.
Yeast 11:419-424(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04694 Genomic DNA. Translation: CAA28399.1.
X89633 Genomic DNA. Translation: CAA61783.1.
Z75186 Genomic DNA. Translation: CAA99504.1.
BK006948 Genomic DNA. Translation: DAA11043.1.
PIRRGBY7K. S67180.
RefSeqNP_014921.1. NM_001183697.1.

3D structure databases

ProteinModelPortalP06174.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34666. 3 interactions.
IntActP06174. 2 interactions.
STRING4932.YOR278W.

Proteomic databases

PaxDbP06174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR278W; YOR278W; YOR278W.
GeneID854452.
KEGGsce:YOR278W.

Organism-specific databases

CYGDYOR278w.
SGDS000005804. HEM4.

Phylogenomic databases

eggNOGCOG1587.
GeneTreeENSGT00390000009853.
HOGENOMHOG000216635.
KOK01719.
OMATGVMESF.
OrthoDBEOG7J44HQ.

Enzyme and pathway databases

BioCycYEAST:YOR278W-MONOMER.
UniPathwayUPA00251; UER00320.

Gene expression databases

GenevestigatorP06174.

Family and domain databases

InterProIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMSSF69618. SSF69618. 1 hit.
ProtoNetSearch...

Other

NextBio976715.
PROP06174.

Entry information

Entry nameHEM4_YEAST
AccessionPrimary (citable) accession number: P06174
Secondary accession number(s): D6W2X7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways