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P06174

- HEM4_YEAST

UniProt

P06174 - HEM4_YEAST

Protein

Uroporphyrinogen-III synthase

Gene

HEM4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

    Catalytic activityi

    Hydroxymethylbilane = uroporphyrinogen III + H2O.

    Pathwayi

    GO - Molecular functioni

    1. uroporphyrinogen-III synthase activity Source: SGD

    GO - Biological processi

    1. heme biosynthetic process Source: SGD
    2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YOR278W-MONOMER.
    UniPathwayiUPA00251; UER00320.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uroporphyrinogen-III synthase (EC:4.2.1.75)
    Short name:
    UROIIIS
    Short name:
    UROS
    Alternative name(s):
    Hydroxymethylbilane hydrolyase [cyclizing]
    Uroporphyrinogen-III cosynthase
    Gene namesi
    Name:HEM4
    Synonyms:ORF1
    Ordered Locus Names:YOR278W
    ORF Names:O5463
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR278w.
    SGDiS000005804. HEM4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275Uroporphyrinogen-III synthasePRO_0000135254Add
    BLAST

    Proteomic databases

    MaxQBiP06174.
    PaxDbiP06174.

    Expressioni

    Gene expression databases

    GenevestigatoriP06174.

    Interactioni

    Protein-protein interaction databases

    BioGridi34666. 3 interactions.
    IntActiP06174. 2 interactions.
    STRINGi4932.YOR278W.

    Structurei

    3D structure databases

    ProteinModelPortaliP06174.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1587.
    GeneTreeiENSGT00390000009853.
    HOGENOMiHOG000216635.
    KOiK01719.
    OMAiTGVMESF.
    OrthoDBiEOG7J44HQ.

    Family and domain databases

    InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
    [Graphical view]
    PfamiPF02602. HEM4. 1 hit.
    [Graphical view]
    SUPFAMiSSF69618. SSF69618. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P06174-1 [UniParc]FASTAAdd to Basket

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    MSSRKKVRVL LLKNKTVPID KYELECRSKA FEPIFVPLIK HTHVIQDFRN    50
    VLNTIPNYLN TINYIIITSQ RTVESLNEAI IPTLTSEQKA ALLSKTVYTV 100
    GPATANFIRR SGFINVKGGE DAGNGSILAD IIIDDLSTDI KACPPSELLF 150
    LVGEIRRDII PKKLHSKGIK VREVVTYKTE ELSDGFKRFI HAMKECDEDE 200
    VFSDWVVVFS PQGTKEITQY LGDSNRLPGS HLRVASIGPT TKKYLDDNDV 250
    TSDVVSPKPD PKSLLDAIEL YQRHK 275
    Length:275
    Mass (Da):30,912
    Last modified:October 1, 1996 - v2
    Checksum:iCDC857C8CFB4E1A7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti231 – 27545HLRVA…YQRHK → IYGLRHRTYH in CAA28399. (PubMed:2834088)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04694 Genomic DNA. Translation: CAA28399.1.
    X89633 Genomic DNA. Translation: CAA61783.1.
    Z75186 Genomic DNA. Translation: CAA99504.1.
    BK006948 Genomic DNA. Translation: DAA11043.1.
    PIRiS67180. RGBY7K.
    RefSeqiNP_014921.1. NM_001183697.1.

    Genome annotation databases

    EnsemblFungiiYOR278W; YOR278W; YOR278W.
    GeneIDi854452.
    KEGGisce:YOR278W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04694 Genomic DNA. Translation: CAA28399.1 .
    X89633 Genomic DNA. Translation: CAA61783.1 .
    Z75186 Genomic DNA. Translation: CAA99504.1 .
    BK006948 Genomic DNA. Translation: DAA11043.1 .
    PIRi S67180. RGBY7K.
    RefSeqi NP_014921.1. NM_001183697.1.

    3D structure databases

    ProteinModelPortali P06174.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34666. 3 interactions.
    IntActi P06174. 2 interactions.
    STRINGi 4932.YOR278W.

    Proteomic databases

    MaxQBi P06174.
    PaxDbi P06174.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR278W ; YOR278W ; YOR278W .
    GeneIDi 854452.
    KEGGi sce:YOR278W.

    Organism-specific databases

    CYGDi YOR278w.
    SGDi S000005804. HEM4.

    Phylogenomic databases

    eggNOGi COG1587.
    GeneTreei ENSGT00390000009853.
    HOGENOMi HOG000216635.
    KOi K01719.
    OMAi TGVMESF.
    OrthoDBi EOG7J44HQ.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00320 .
    BioCyci YEAST:YOR278W-MONOMER.

    Miscellaneous databases

    NextBioi 976715.
    PROi P06174.

    Gene expression databases

    Genevestigatori P06174.

    Family and domain databases

    InterProi IPR003754. 4pyrrol_synth_uPrphyn_synth.
    [Graphical view ]
    Pfami PF02602. HEM4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69618. SSF69618. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a nuclear yeast gene involved in the mitochondrial import of cytoplasmically synthesized precursor proteins."
      Langgut W., Entrup R., Schweizer E.
      Curr. Genet. 11:177-184(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae."
      Cheret G., Bernardi A., Sor F.J.
      Yeast 12:1059-1064(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Isolation of the gene HEM4 encoding uroporphyrinogen III synthase in Saccharomyces cerevisiae."
      Amillet J.-M., Labbe-Bois R.
      Yeast 11:419-424(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHEM4_YEAST
    AccessioniPrimary (citable) accession number: P06174
    Secondary accession number(s): D6W2X7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1360 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was originally thought to be involved in mitochondrial import.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3