P06174 (HEM4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uroporphyrinogen-III synthase
Short name=UROIIIS
Short name=UROS
EC=4.2.1.75

Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase

Gene names

Name:	HEM4
Synonyms:	ORF1
Ordered Locus Names:	YOR278W
ORF Names:	O5463

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III By similarity.
Catalytic activity	Hydroxymethylbilane = uroporphyrinogen III + H2O.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.
Miscellaneous	Present with 1360 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the uroporphyrinogen-III synthase family.
Caution	Was originally (Ref.1) thought to be involved in mitochondrial import.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heme biosynthetic process Inferred from mutant phenotype PubMed 18042043. Source: SGD protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	uroporphyrinogen-III synthase activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Uroporphyrinogen-III synthase		PRO_0000135254
Experimental info
	Sequence conflict	231 – 275	45	HLRVA…YQRHK → IYGLRHRTYH in CAA28399. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P06174 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: CDC857C8CFB4E1A7	FASTA	275	30,912
	10 20 30 40 50 60 MSSRKKVRVL LLKNKTVPID KYELECRSKA FEPIFVPLIK HTHVIQDFRN VLNTIPNYLN 70 80 90 100 110 120 TINYIIITSQ RTVESLNEAI IPTLTSEQKA ALLSKTVYTV GPATANFIRR SGFINVKGGE 130 140 150 160 170 180 DAGNGSILAD IIIDDLSTDI KACPPSELLF LVGEIRRDII PKKLHSKGIK VREVVTYKTE 190 200 210 220 230 240 ELSDGFKRFI HAMKECDEDE VFSDWVVVFS PQGTKEITQY LGDSNRLPGS HLRVASIGPT 250 260 270 TKKYLDDNDV TSDVVSPKPD PKSLLDAIEL YQRHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a nuclear yeast gene involved in the mitochondrial import of cytoplasmically synthesized precursor proteins." Langgut W., Entrup R., Schweizer E. Curr. Genet. 11:177-184(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae." Cheret G., Bernardi A., Sor F.J. Yeast 12:1059-1064(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Isolation of the gene HEM4 encoding uroporphyrinogen III synthase in Saccharomyces cerevisiae." Amillet J.-M., Labbe-Bois R. Yeast 11:419-424(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04694 Genomic DNA. Translation: CAA28399.1. X89633 Genomic DNA. Translation: CAA61783.1. Z75186 Genomic DNA. Translation: CAA99504.1. BK006948 Genomic DNA. Translation: DAA11043.1.
PIR	RGBY7K. S67180.
RefSeq	NP_014921.1. NM_001183697.1.
3D structure databases
ProteinModelPortal	P06174.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06174. 2 interactions.
STRING	4932.YOR278W.
Proteomic databases
PaxDb	P06174.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR278W; YOR278W; YOR278W.
GeneID	854452.
KEGG	sce:YOR278W.
Organism-specific databases
CYGD	YOR278w.
SGD	S000005804. HEM4.
Phylogenomic databases
eggNOG	COG1587.
GeneTree	ENSGT00390000009853.
HOGENOM	HOG000216635.
KO	K01719.
OMA	FSPTGCD.
OrthoDB	EOG43NBP2.
Enzyme and pathway databases
UniPathway	UPA00251; UER00320.
Gene expression databases
Genevestigator	P06174.
GermOnline	YOR278W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003754. 4pyrrol_synth_uPrphyn_synth. [Graphical view]
Pfam	PF02602. HEM4. 1 hit. [Graphical view]
SUPFAM	SSF69618. HEM4_synth. 1 hit.
ProtoNet	Search...
Other
NextBio	976715.

Entry information

Entry name

HEM4_YEAST

Accession

Primary (citable) accession number: P06174
Secondary accession number(s): D6W2X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program