Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Uroporphyrinogen-III synthase

Gene

HEM4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi

GO - Molecular functioni

  1. uroporphyrinogen-III synthase activity Source: SGD

GO - Biological processi

  1. heme biosynthetic process Source: SGD
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YOR278W-MONOMER.
ReactomeiREACT_347076. Heme biosynthesis.
UniPathwayiUPA00251; UER00320.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase (EC:4.2.1.75)
Short name:
UROIIIS
Short name:
UROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:HEM4
Synonyms:ORF1
Ordered Locus Names:YOR278W
ORF Names:O5463
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOR278w.
SGDiS000005804. HEM4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Uroporphyrinogen-III synthasePRO_0000135254Add
BLAST

Proteomic databases

MaxQBiP06174.
PaxDbiP06174.

Expressioni

Gene expression databases

GenevestigatoriP06174.

Interactioni

Protein-protein interaction databases

BioGridi34666. 4 interactions.
IntActiP06174. 2 interactions.
STRINGi4932.YOR278W.

Structurei

3D structure databases

ProteinModelPortaliP06174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1587.
GeneTreeiENSGT00390000009853.
HOGENOMiHOG000216635.
InParanoidiP06174.
KOiK01719.
OMAiTGVMESF.
OrthoDBiEOG7J44HQ.

Family and domain databases

InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.

Sequencei

Sequence statusi: Complete.

P06174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRKKVRVL LLKNKTVPID KYELECRSKA FEPIFVPLIK HTHVIQDFRN
60 70 80 90 100
VLNTIPNYLN TINYIIITSQ RTVESLNEAI IPTLTSEQKA ALLSKTVYTV
110 120 130 140 150
GPATANFIRR SGFINVKGGE DAGNGSILAD IIIDDLSTDI KACPPSELLF
160 170 180 190 200
LVGEIRRDII PKKLHSKGIK VREVVTYKTE ELSDGFKRFI HAMKECDEDE
210 220 230 240 250
VFSDWVVVFS PQGTKEITQY LGDSNRLPGS HLRVASIGPT TKKYLDDNDV
260 270
TSDVVSPKPD PKSLLDAIEL YQRHK
Length:275
Mass (Da):30,912
Last modified:September 30, 1996 - v2
Checksum:iCDC857C8CFB4E1A7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti231 – 27545HLRVA…YQRHK → IYGLRHRTYH in CAA28399 (PubMed:2834088).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04694 Genomic DNA. Translation: CAA28399.1.
X89633 Genomic DNA. Translation: CAA61783.1.
Z75186 Genomic DNA. Translation: CAA99504.1.
BK006948 Genomic DNA. Translation: DAA11043.1.
PIRiS67180. RGBY7K.
RefSeqiNP_014921.1. NM_001183697.1.

Genome annotation databases

EnsemblFungiiYOR278W; YOR278W; YOR278W.
GeneIDi854452.
KEGGisce:YOR278W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04694 Genomic DNA. Translation: CAA28399.1.
X89633 Genomic DNA. Translation: CAA61783.1.
Z75186 Genomic DNA. Translation: CAA99504.1.
BK006948 Genomic DNA. Translation: DAA11043.1.
PIRiS67180. RGBY7K.
RefSeqiNP_014921.1. NM_001183697.1.

3D structure databases

ProteinModelPortaliP06174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34666. 4 interactions.
IntActiP06174. 2 interactions.
STRINGi4932.YOR278W.

Proteomic databases

MaxQBiP06174.
PaxDbiP06174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR278W; YOR278W; YOR278W.
GeneIDi854452.
KEGGisce:YOR278W.

Organism-specific databases

CYGDiYOR278w.
SGDiS000005804. HEM4.

Phylogenomic databases

eggNOGiCOG1587.
GeneTreeiENSGT00390000009853.
HOGENOMiHOG000216635.
InParanoidiP06174.
KOiK01719.
OMAiTGVMESF.
OrthoDBiEOG7J44HQ.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00320.
BioCyciYEAST:YOR278W-MONOMER.
ReactomeiREACT_347076. Heme biosynthesis.

Miscellaneous databases

NextBioi976715.
PROiP06174.

Gene expression databases

GenevestigatoriP06174.

Family and domain databases

InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a nuclear yeast gene involved in the mitochondrial import of cytoplasmically synthesized precursor proteins."
    Langgut W., Entrup R., Schweizer E.
    Curr. Genet. 11:177-184(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae."
    Cheret G., Bernardi A., Sor F.J.
    Yeast 12:1059-1064(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Isolation of the gene HEM4 encoding uroporphyrinogen III synthase in Saccharomyces cerevisiae."
    Amillet J.-M., Labbe-Bois R.
    Yeast 11:419-424(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHEM4_YEAST
AccessioniPrimary (citable) accession number: P06174
Secondary accession number(s): D6W2X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 31, 1987
Last sequence update: September 30, 1996
Last modified: March 31, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be involved in mitochondrial import.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.