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Protein

Ketol-acid reductoisomerase, mitochondrial

Gene

ILV5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 883000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit, mitochondrial (ILV6), Acetolactate synthase catalytic subunit, mitochondrial (ILV2)
  2. Ketol-acid reductoisomerase, mitochondrial (ILV5)
  3. Dihydroxy-acid dehydratase, mitochondrial (ILV3)
  4. Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit, mitochondrial (ILV6), Acetolactate synthase catalytic subunit, mitochondrial (ILV2)
  2. Ketol-acid reductoisomerase, mitochondrial (ILV5)
  3. Dihydroxy-acid dehydratase, mitochondrial (ILV3)
  4. Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei171Sequence analysis1
Metal bindingi255Magnesium 1PROSITE-ProRule annotation1
Metal bindingi255Magnesium 2PROSITE-ProRule annotation1
Metal bindingi259Magnesium 1PROSITE-ProRule annotation1
Metal bindingi291Magnesium 2PROSITE-ProRule annotation1
Metal bindingi295Magnesium 2PROSITE-ProRule annotation1
Binding sitei317SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi84 – 93NADPSequence analysis10
Nucleotide bindingi108 – 113NADPBy similarity6
Nucleotide bindingi146 – 150NADPBy similarity5

GO - Molecular functioni

  • double-stranded DNA binding Source: SGD
  • ketol-acid reductoisomerase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • branched-chain amino acid biosynthetic process Source: SGD
  • isoleucine biosynthetic process Source: UniProtKB-UniPathway
  • mitochondrial genome maintenance Source: SGD
  • valine biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, NADP

Enzyme and pathway databases

BioCyciYEAST:YLR355C-MONOMER
UniPathwayiUPA00047; UER00056
UPA00049; UER00060

Protein family/group databases

MoonProtiP06168

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase, mitochondrial (EC:1.1.1.86)
Alternative name(s):
Acetohydroxy-acid reductoisomerase
Alpha-keto-beta-hydroxylacyl reductoisomerase
Gene namesi
Name:ILV5
Ordered Locus Names:YLR355C
ORF Names:L9638.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR355C
SGDiS000004347 ILV5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionSequence analysisAdd BLAST47
ChainiPRO_000001563448 – 395Ketol-acid reductoisomerase, mitochondrialAdd BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei355PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06168
PaxDbiP06168
PRIDEiP06168
TopDownProteomicsiP06168

2D gel databases

SWISS-2DPAGEiP06168

PTM databases

CarbonylDBiP06168
iPTMnetiP06168

Interactioni

Protein-protein interaction databases

BioGridi31617, 121 interactors
DIPiDIP-6463N
IntActiP06168, 38 interactors
MINTiP06168
STRINGi4932.YLR355C

Structurei

3D structure databases

ProteinModelPortaliP06168
SMRiP06168
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 246KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST190
Domaini247 – 394KARI C-terminal knottedPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni363 – 395HydrophilicAdd BLAST33

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000016231
InParanoidiP06168
KOiK00053
OMAiRAMFSWL
OrthoDBiEOG092C2OSA

Family and domain databases

Gene3Di1.10.1040.10, 3 hits
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR013023 KARI
IPR000506 KARI_C
IPR013116 KARI_N
IPR016207 KetolA_reductoisomerase_fun
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR21371 PTHR21371, 1 hit
PfamiView protein in Pfam
PF01450 IlvC, 1 hit
PF07991 IlvN, 1 hit
PIRSFiPIRSF000119 Ilv5_fungal, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00465 ilvC, 1 hit
PROSITEiView protein in PROSITE
PS51851 KARI_C, 1 hit
PS51850 KARI_N, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRTQAARLI CNSRVITAKR TFALATRAAA YSRPAARFVK PMITTRGLKQ
60 70 80 90 100
INFGGTVETV YERADWPREK LLDYFKNDTF ALIGYGSQGY GQGLNLRDNG
110 120 130 140 150
LNVIIGVRKD GASWKAAIED GWVPGKNLFT VEDAIKRGSY VMNLLSDAAQ
160 170 180 190 200
SETWPAIKPL LTKGKTLYFS HGFSPVFKDL THVEPPKDLD VILVAPKGSG
210 220 230 240 250
RTVRSLFKEG RGINSSYAVW NDVTGKAHEK AQALAVAIGS GYVYQTTFER
260 270 280 290 300
EVNSDLYGER GCLMGGIHGM FLAQYDVLRE NGHSPSEAFN ETVEEATQSL
310 320 330 340 350
YPLIGKYGMD YMYDACSTTA RRGALDWYPI FKNALKPVFQ DLYESTKNGT
360 370 380 390
ETKRSLEFNS QPDYREKLEK ELDTIRNMEI WKVGKEVRKL RPENQ
Length:395
Mass (Da):44,368
Last modified:January 1, 1988 - v1
Checksum:iD76419A6AD68E85E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04969 Genomic DNA Translation: CAA28643.1
U19102 Genomic DNA Translation: AAB67753.1
BK006945 Genomic DNA Translation: DAA09659.1
PIRiA24709
RefSeqiNP_013459.1, NM_001182244.1

Genome annotation databases

EnsemblFungiiYLR355C; YLR355C; YLR355C
GeneIDi851069
KEGGisce:YLR355C

Similar proteinsi

Entry informationi

Entry nameiILV5_YEAST
AccessioniPrimary (citable) accession number: P06168
Secondary accession number(s): D6VYZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: May 23, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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