Reviewed,
UniProtKB/Swiss-Prot P06168 (ILV5_YEAST)
Last modified
June 16, 2009.
Version 93.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ketol-acid reductoisomerase, mitochondrial EC=1.1.1.86 Alternative name(s): Acetohydroxy-acid reductoisomerase Alpha-keto-beta-hydroxylacil reductoisomerase | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 395 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. |
| Cofactor | Magnesium. |
| Pathway | Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. |
| Subcellular location | |
| Miscellaneous | Present with 883000 molecules/cell in log phase SD medium. Ref.5 |
| Sequence similarities | Belongs to the ketol-acid reductoisomerase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Branched-chain amino acid biosynthesis |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | Magnesium NADP |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | branched chain family amino acid biosynthetic process Traceable author statement. Source: SGD mitochondrial genome maintenanceInferred from mutant phenotype. Source: SGD oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | mitochondrial nucleoid Inferred from direct assay. Source: SGD |
| Molecular function | coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activityTraceable author statement. Source: SGD magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 47 | 47 | Mitochondrion Potential | ||||||
| Chain | 48 – 395 | 348 | Ketol-acid reductoisomerase, mitochondrial | PRO_0000015634 | |||||
Regions | |||||||||
| Nucleotide binding | 84 – 93 | 10 | NADP Potential | ||||||
| Region | 363 – 395 | 33 | Hydrophilic | ||||||
Sites | |||||||||
| Active site | 171 | 1 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 85 | 1 | Phosphotyrosine Ref.6 | ||||||
| Modified residue | 317 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 318 | 1 | Phosphothreonine Ref.6 | ||||||
| Modified residue | 355 | 1 | Phosphoserine Ref.6 | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| X04969 Genomic DNA. Translation: CAA28643.1. U19102 Genomic DNA. Translation: AAB67753.1. | |
| PIR | A24709. |
| RefSeq | NP_013459.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1QMG based on UniProtKB Q01292. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:6463N. |
| IntAct | P06168. 14 interactions. |
2-D gel databases | |
| SWISS-2DPAGE | P06168. |
Proteomic databases | |
| PeptideAtlas | P06168. |
| PRIDE | P06168. |
Genome annotation databases | |
| Ensembl | YLR355C. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 851069. |
| GenomeReviews | Gene locus YLR355C in contig Y13138_GR. |
| KEGG | sce:YLR355C. |
| NMPDR | fig|4932.3.peg.4481. |
Organism-specific databases | |
| CYGD | YLR355c. |
| SGD | S000004347. ILV5. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P06168. |
| OMA | P06168. DGWVPGE. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.86. 250. |
Gene expression databases | |
| ArrayExpress | P06168. |
| GermOnline | YLR355C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR016207. KetolA_reductoisomerase_fun. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit. |
| PANTHER | PTHR21371. AcH_isomrdctse. 1 hit. |
| Pfam | PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view] |
| PIRSF | PIRSF000119. Ilv5_fungal. 1 hit. |
| TIGRFAMs | TIGR00465. ilvC. 1 hit. |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 967711. |
Entry information
| Entry name | ILV5_YEAST | ||||||||
| Accession | Primary (citable) accession number: P06168 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


