Reviewed, UniProtKB/Swiss-Prot P06167 (HN_PI3B)

Names and origin

Protein names

Recommended name:
    Hemagglutinin-neuraminidase
    EC=3.2.1.18

Gene names

Name:

HN

Organism

Bovine parainfluenza 3 virus (BPIV-3)

Taxonomic identifier

11215 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Respirovirus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Cellular component	Cell membrane Envelope protein Membrane Virion
Domain	Signal-anchor Transmembrane
Molecular function	Hemagglutinin Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Hemagglutinin-neuraminidase		PRO_0000142624
Regions
	Topological domain	1 – 30	30	Cytoplasmic Potential
	Transmembrane	31 – 51	21	Potential
	Topological domain	52 – 572	521	Extracellular Potential
Amino acid modifications
	Glycosylation	308	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	351	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	448	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	523	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	570	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P06167-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: DDF84E90C4EC87B4	FASTA	572	64,590
	10 20 30 40 50 60 MEYWKHTNST KDTNNELGTT RDRHSSKATN IIMYIFWTTT STILSVIFIM ILINLIQENN 70 80 90 100 110 120 HNKLMLQEIR KEFAAIDTKI QKTSDDIGTS IQSGINTRLL TIQSHVQNYI PLSLTQQMSD 130 140 150 160 170 180 LRKFINDLTT KREHQEVPIQ RMTHDSGIEP LNPDKFWRCT SGNPSLTSSP KIRLIPGPGL 190 200 210 220 230 240 LATSTTVNGC IRLPSLAINN LIYAYTSNLI TQGCQDIGKS YQVLQIGIIT INSDLVPDLN 250 260 270 280 290 300 PRVTHTFNID DNRKSCSLAL LNTDVYQLCS TPKVDERSDY ASTGIEDIVL DIVTSNGLII 310 320 330 340 350 360 TTRFTNNNIT FDKPYAALYP SVGPGIYYKD KVIFLGYGGL EHEENGDVIC NTTGCPGKTQ 370 380 390 400 410 420 RDCNQASYSP WFSNRRMVNS IIVVDKGIDT TFSLRVWTIP MRQNYWGSEG RLLLLGDRIY 430 440 450 460 470 480 IYTRSTSWHS KLQLGVIDIS DFNNIRINWT WHNVLSRPGN DECPWGHSCP DGCITGVYTD 490 500 510 520 530 540 AYPLNPSGSI VSSVILDSQK SRENPIITYS TATNRVNELA IYNRTLPAAY TTTNCITHYD 550 560 570 KGYCFHIVEI NHRSLNTFQP MLFKTEVPKN CS

References

[1]	"Nucleotide sequence of the bovine parainfluenza 3 virus genome: the genes of the F and HN glycoproteins." Suzu S., Sakai Y., Shioda T., Shibuta H. Nucleic Acids Res. 15:2945-2958(1987) [PubMed: 3031615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: 910N.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00114 Genomic RNA. Translation: CAA68298.1. D84095 Genomic RNA. Translation: BAA12218.1.
3D structure databases
SMR	P06167. Positions 142-572.
ModBase	Search...
Protein family/group databases
CAZy	GH83. Glycoside Hydrolase Family 83.
Family and domain databases
InterPro	IPR000665. Hemagglutn-neuramid_glycoprot. IPR016285. Hemagglutn-neuramid_paramyxo. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_PI3B

Accession

Primary (citable) accession number: P06167

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)