ID   LDHA_MOUSE              Reviewed;         332 AA.
AC   P06151;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 99.
DE   RecName: Full=L-lactate dehydrogenase A chain;
DE            Short=LDH-A;
DE            EC=1.1.1.27;
DE   AltName: Full=LDH muscle subunit;
DE            Short=LDH-M;
GN   Name=Ldha; Synonyms=Ldh-1, Ldh1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C57BL/10; TISSUE=Liver;
RX   MEDLINE=87248042; PubMed=3036647;
RA   Fukasawa K.M., Li S.S.-L.;
RT   "Complete nucleotide sequence of the mouse lactate dehydrogenase-A
RT   functional gene: comparison of the exon-intron organization of
RT   dehydrogenase genes.";
RL   Genetics 116:99-105(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=85203900; PubMed=3996406;
RX   DOI=10.1111/j.1432-1033.1985.tb08914.x;
RA   Li S.S.-L., Tiano H.F., Fukasawa K.M., Yagi K., Shimizu M.,
RA   Sharief F.S., Nakashima Y., Pan Y.E.;
RT   "Protein structure and gene organization of mouse lactate
RT   dehydrogenase-A isozyme.";
RL   Eur. J. Biochem. 149:215-225(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=DBA/2J;
RA   Hiraoka Y., Li S.S.-L.;
RT   "Lactate dehydrogenase-A mRNAs in mouse testis and somatic tissues
RT   containing different 5' noncoding sequences.";
RL   J. Genet. Mol. Biol. 1:1-6(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
RX   MEDLINE=86295627; PubMed=3017306;
RA   Fukasawa K.M., Li S.S.-L.;
RT   "Nucleotide sequence of the putative regulatory region of mouse
RT   lactate dehydrogenase-A gene.";
RL   Biochem. J. 235:435-439(1986).
RN   [5]
RP   PROTEIN SEQUENCE OF 6-14; 23-73; 82-99; 107-126; 133-149; 158-169;
RP   172-177; 213-228; 233-243; 246-265; 269-315 AND 319-328, AND MASS
RP   SPECTROMETRY.
RC   STRAIN=C57BL/6, and OF1; TISSUE=Brain, and Hippocampus;
RA   Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B.,
RA   Chen W.-Q.;
RL   Submitted (JAN-2009) to UniProtKB.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 202-332.
RX   MEDLINE=85285968; PubMed=2993055; DOI=10.1016/0020-711X(85)90298-8;
RA   Akai K., Yagi K., Tiano H.F., Pan Y.-C.E., Shimizu M., Fong K.,
RA   Jungmann R.A., Li S.S.-L.;
RT   "Isolation and characterization of a cDNA and a pseudogene for mouse
RT   lactate dehydrogenase-A isozyme.";
RL   Int. J. Biochem. 17:645-648(1985).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Mast cell;
RX   PubMed=17947660;
RA   Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y.,
RA   Kawakami T., Salomon A.R.;
RT   "Quantitative time-resolved phosphoproteomic analysis of mast cell
RT   signaling.";
RL   J. Immunol. 179:5864-5876(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Brain;
RX   PubMed=18034455; DOI=10.1021/pr0701254;
RA   Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
RT   "Large-scale identification and evolution indexing of tyrosine
RT   phosphorylation sites from murine brain.";
RL   J. Proteome Res. 7:311-318(2008).
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- INTERACTION:
CC       Q60668:Hnrnpd; NbExp=2; IntAct=EBI-444940, EBI-299932;
CC       Q9JHG7:Pik3cg; NbExp=2; IntAct=EBI-444940, EBI-644372;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; Y00309; CAA68410.1; -; Genomic_DNA.
DR   EMBL; X02520; CAA26360.1; -; Genomic_DNA.
DR   EMBL; X02521; CAA26360.1; JOINED; Genomic_DNA.
DR   EMBL; X02522; CAA26360.1; JOINED; Genomic_DNA.
DR   EMBL; X02523; CAA26360.1; JOINED; Genomic_DNA.
DR   EMBL; X02524; CAA26360.1; JOINED; Genomic_DNA.
DR   EMBL; X02525; CAA26360.1; JOINED; Genomic_DNA.
DR   EMBL; X02526; CAA26360.1; JOINED; Genomic_DNA.
DR   EMBL; U13687; AAA21466.1; -; mRNA.
DR   EMBL; X03753; CAA27387.1; -; Genomic_DNA.
DR   EMBL; M17516; AAA39424.1; -; mRNA.
DR   IPI; IPI00319994; -.
DR   PIR; A25205; DEMSLM.
DR   PIR; I48240; I48240.
DR   RefSeq; NP_001129541.1; -.
DR   RefSeq; NP_034829.1; -.
DR   UniGene; Mm.26751; -.
DR   UniGene; Mm.29324; -.
DR   HSSP; P00338; 1I10.
DR   SMR; P06151; 2-332.
DR   IntAct; P06151; 4.
DR   PhosphoSite; P06151; -.
DR   SWISS-2DPAGE; P06151; -.
DR   REPRODUCTION-2DPAGE; P06151; -.
DR   PRIDE; P06151; -.
DR   Ensembl; ENSMUSG00000063229; Mus musculus.
DR   GeneID; 16828; -.
DR   KEGG; mmu:16828; -.
DR   MGI; MGI:96759; Ldha.
DR   HOGENOM; P06151; -.
DR   HOVERGEN; P06151; -.
DR   BRENDA; 1.1.1.27; 244.
DR   NextBio; 290734; -.
DR   ArrayExpress; P06151; -.
DR   Bgee; P06151; -.
DR   CleanEx; MM_LDHA; -.
DR   GermOnline; ENSMUSG00000063229; Mus musculus.
DR   GO; GO:0005829; C:cytosol; IDA:MGI.
DR   GO; GO:0019861; C:flagellum; IDA:MGI.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IDA:MGI.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:InterPro.
DR   GO; GO:0031668; P:cellular response to extracellular stimulus; IDA:MGI.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR011304; L-lactate_DH.
DR   InterPro; IPR018177; L-lactate_DH_AS.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cytoplasm; Direct protein sequencing; Glycolysis; NAD;
KW   Oxidoreductase; Phosphoprotein.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    332       L-lactate dehydrogenase A chain.
FT                                /FTId=PRO_0000168414.
FT   NP_BIND      29     57       NAD (By similarity).
FT   ACT_SITE    193    193       Proton acceptor (By similarity).
FT   BINDING      99     99       NAD (By similarity).
FT   BINDING     106    106       Substrate (By similarity).
FT   BINDING     138    138       NAD or substrate (By similarity).
FT   BINDING     169    169       Substrate (By similarity).
FT   BINDING     248    248       Substrate (By similarity).
FT   MOD_RES       2      2       N-acetylalanine (By similarity).
FT   MOD_RES     239    239       Phosphotyrosine.
FT   CONFLICT     11     11       N -> I (in Ref. 2).
SQ   SEQUENCE   332 AA;  36499 MW;  5AEB41E1E0B95100 CRC64;
     MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG
     EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI
     IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV
     HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE
     VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI
     SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF
//