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Protein

L-lactate dehydrogenase A chain

Gene

Ldha

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhb), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase B chain (Ldhb), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldhal6b), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase A chain (Ldha), L-lactate dehydrogenase (Ldhb), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase C chain (Ldhc), L-lactate dehydrogenase (Ldhc)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99NADBy similarity1
Binding sitei106SubstrateBy similarity1
Binding sitei138NAD or substrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei193Proton acceptorBy similarity1
Binding sitei248SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 57NADBy similarityAdd BLAST29

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: MGI
  • lactate dehydrogenase activity Source: MGI
  • L-lactate dehydrogenase activity Source: MGI

GO - Biological processi

  • cellular response to extracellular stimulus Source: MGI
  • glucose catabolic process to lactate via pyruvate Source: MGI
  • lactate biosynthetic process from pyruvate Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-70268. Pyruvate metabolism.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Alternative name(s):
LDH muscle subunit
Short name:
LDH-M
Gene namesi
Name:Ldha
Synonyms:Ldh-1, Ldh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96759. Ldha.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • mitochondrion Source: MGI
  • nucleus Source: MGI
  • sperm fibrous sheath Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001684142 – 332L-lactate dehydrogenase A chainAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei5N6-acetyllysine; alternateCombined sources1
Modified residuei5N6-succinyllysine; alternateCombined sources1
Modified residuei14N6-acetyllysineBy similarity1
Modified residuei57N6-acetyllysineBy similarity1
Modified residuei81N6-acetyllysineCombined sources1
Modified residuei118N6-acetyllysine; alternateCombined sources1
Modified residuei118N6-succinyllysine; alternateCombined sources1
Modified residuei126N6-acetyllysineCombined sources1
Modified residuei224N6-acetyllysineCombined sources1
Modified residuei232N6-acetyllysineCombined sources1
Modified residuei239PhosphotyrosineCombined sources1
Modified residuei243N6-acetyllysineCombined sources1
Modified residuei309PhosphothreonineBy similarity1
Modified residuei318N6-acetyllysine; alternateCombined sources1
Modified residuei318N6-succinyllysine; alternateCombined sources1
Modified residuei322PhosphothreonineBy similarity1

Post-translational modificationi

ISGylated.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP06151.
PaxDbiP06151.
PeptideAtlasiP06151.
PRIDEiP06151.
TopDownProteomicsiP06151.

2D gel databases

REPRODUCTION-2DPAGEP06151.
SWISS-2DPAGEP06151.
UCD-2DPAGEP06151.

PTM databases

iPTMnetiP06151.
PhosphoSitePlusiP06151.
SwissPalmiP06151.

Expressioni

Gene expression databases

BgeeiENSMUSG00000063229.
CleanExiMM_LDHA.
ExpressionAtlasiP06151. baseline and differential.
GenevisibleiP06151. MM.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HnrnpdQ606682EBI-444940,EBI-299932

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201127. 9 interactors.
IntActiP06151. 22 interactors.
MINTiMINT-1612559.
STRINGi10090.ENSMUSP00000103267.

Structurei

3D structure databases

ProteinModelPortaliP06151.
SMRiP06151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP06151.
KOiK00016.
PhylomeDBiP06151.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL
60 70 80 90 100
VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ
110 120 130 140 150
QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI
160 170 180 190 200
SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV
210 220 230 240 250
WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW
260 270 280 290 300
AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI
310 320 330
SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF
Length:332
Mass (Da):36,499
Last modified:January 23, 2007 - v3
Checksum:i5AEB41E1E0B95100
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11N → I (PubMed:3996406).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00309 Genomic DNA. Translation: CAA68410.1.
X02520
, X02521, X02522, X02523, X02524, X02525, X02526 Genomic DNA. Translation: CAA26360.1.
U13687 mRNA. Translation: AAA21466.1.
X03753 Genomic DNA. Translation: CAA27387.1.
M17516 mRNA. Translation: AAA39424.1.
CCDSiCCDS21289.1.
PIRiA25205. DEMSLM.
I48240.
RefSeqiNP_034829.1. NM_010699.2.
UniGeneiMm.29324.

Genome annotation databases

EnsembliENSMUST00000005051; ENSMUSP00000103267; ENSMUSG00000063229.
ENSMUST00000048209; ENSMUSP00000036386; ENSMUSG00000063229.
GeneIDi16828.
KEGGimmu:16828.
UCSCiuc009gzm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00309 Genomic DNA. Translation: CAA68410.1.
X02520
, X02521, X02522, X02523, X02524, X02525, X02526 Genomic DNA. Translation: CAA26360.1.
U13687 mRNA. Translation: AAA21466.1.
X03753 Genomic DNA. Translation: CAA27387.1.
M17516 mRNA. Translation: AAA39424.1.
CCDSiCCDS21289.1.
PIRiA25205. DEMSLM.
I48240.
RefSeqiNP_034829.1. NM_010699.2.
UniGeneiMm.29324.

3D structure databases

ProteinModelPortaliP06151.
SMRiP06151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201127. 9 interactors.
IntActiP06151. 22 interactors.
MINTiMINT-1612559.
STRINGi10090.ENSMUSP00000103267.

PTM databases

iPTMnetiP06151.
PhosphoSitePlusiP06151.
SwissPalmiP06151.

2D gel databases

REPRODUCTION-2DPAGEP06151.
SWISS-2DPAGEP06151.
UCD-2DPAGEP06151.

Proteomic databases

EPDiP06151.
PaxDbiP06151.
PeptideAtlasiP06151.
PRIDEiP06151.
TopDownProteomicsiP06151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005051; ENSMUSP00000103267; ENSMUSG00000063229.
ENSMUST00000048209; ENSMUSP00000036386; ENSMUSG00000063229.
GeneIDi16828.
KEGGimmu:16828.
UCSCiuc009gzm.2. mouse.

Organism-specific databases

CTDi3939.
MGIiMGI:96759. Ldha.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP06151.
KOiK00016.
PhylomeDBiP06151.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-MMU-70268. Pyruvate metabolism.

Miscellaneous databases

ChiTaRSiLdha. mouse.
PROiP06151.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063229.
CleanExiMM_LDHA.
ExpressionAtlasiP06151. baseline and differential.
GenevisibleiP06151. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDHA_MOUSE
AccessioniPrimary (citable) accession number: P06151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 173 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.