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Reviewed, UniProtKB/Swiss-Prot P06151 (LDHA_MOUSE)

Last modified June 16, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    L-lactate dehydrogenase A chain
      Short name=LDH-A
    EC=1.1.1.27
Alternative name(s):
    LDH muscle subunit
      Short name=LDH-M
Gene names
Name: Ldha
Synonyms: Ldh-1, Ldh1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

(S)-lactate + NAD+ = pyruvate + NADH.

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMAcetylation
Phosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processanaerobic glycolysis

Inferred from electronic annotation. Source: InterPro

cellular response to extracellular stimulus

Inferred from direct assay. Source: MGI

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytosol

Inferred from direct assay. Source: MGI

flagellum

Inferred from direct assay. Source: MGI

   Molecular functionL-lactate dehydrogenase activity

Inferred from direct assay. Source: MGI

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HnrnpdQ606682EBI-444940,EBI-299932
Pik3cgQ9JHG72EBI-444940,EBI-644372

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 332331L-lactate dehydrogenase A chain
PRO_0000168414

Regions

Nucleotide binding29 – 5729NAD By similarity

Sites

Active site1931Proton acceptor By similarity
Binding site991NAD By similarity
Binding site1061Substrate By similarity
Binding site1381NAD or substrate By similarity
Binding site1691Substrate By similarity
Binding site2481Substrate By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue2391Phosphotyrosine Ref.7 Ref.8

Experimental info

Sequence conflict111N → I Ref.2

Sequences

Sequence LengthMass (Da)Tools
P06151-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 5AEB41E1E0B95100

FASTA33236,499
        10         20         30         40         50         60 
MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG 

        70         80         90        100        110        120 
EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI 

       130        140        150        160        170        180 
IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV 

       190        200        210        220        230        240 
HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE 

       250        260        270        280        290        300 
VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI 

       310        320        330 
SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of the mouse lactate dehydrogenase-A functional gene: comparison of the exon-intron organization of dehydrogenase genes."
Fukasawa K.M., Li S.S.-L.
Genetics 116:99-105(1987) [PubMed: 3036647] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57BL/10.
Tissue: Liver.
[2]"Protein structure and gene organization of mouse lactate dehydrogenase-A isozyme."
Li S.S.-L., Tiano H.F., Fukasawa K.M., Yagi K., Shimizu M., Sharief F.S., Nakashima Y., Pan Y.E.
Eur. J. Biochem. 149:215-225(1985) [PubMed: 3996406] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Lactate dehydrogenase-A mRNAs in mouse testis and somatic tissues containing different 5' noncoding sequences."
Hiraoka Y., Li S.S.-L.
J. Genet. Mol. Biol. 1:1-6(1990)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: DBA/2J.
[4]"Nucleotide sequence of the putative regulatory region of mouse lactate dehydrogenase-A gene."
Fukasawa K.M., Li S.S.-L.
Biochem. J. 235:435-439(1986) [PubMed: 3017306] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
[5]Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 6-14; 23-73; 82-99; 107-126; 133-149; 158-169; 172-177; 213-228; 233-243; 246-265; 269-315 AND 319-328, MASS SPECTROMETRY.
Strain: C57BL/6 and OF1.
Tissue: Brain and Hippocampus.
[6]"Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme."
Akai K., Yagi K., Tiano H.F., Pan Y.-C.E., Shimizu M., Fong K., Jungmann R.A., Li S.S.-L.
Int. J. Biochem. 17:645-648(1985) [PubMed: 2993055] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 202-332.
[7]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, MASS SPECTROMETRY.
Tissue: Mast cell.
[8]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, MASS SPECTROMETRY.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

Y00309 Genomic DNA. Translation: CAA68410.1.
X02520 expand/collapse EMBL AC list , X02521, X02522, X02523, X02524, X02525, X02526 Genomic DNA. Translation: CAA26360.1.
U13687 mRNA. Translation: AAA21466.1.
X03753 Genomic DNA. Translation: CAA27387.1.
M17516 mRNA. Translation: AAA39424.1.
IPIIPI00319994.
PIRDEMSLM. A25205.
I48240.
RefSeqNP_001129541.1.
NP_034829.1.
UniGeneMm.26751
Mm.29324

3D structure databases

HSSPHSSP built from PDB template 1I10 based on UniProtKB P00338.
SMRP06151. Positions 2-332.
ModBaseSearch...

Protein-protein interaction databases

IntActP06151. 4 interactions.

PTM databases

PhosphoSiteP06151.

2-D gel databases

SWISS-2DPAGEP06151.
REPRODUCTION-2DPAGEP06151.

Proteomic databases

PRIDEP06151.

Genome annotation databases

EnsemblENSMUSG00000063229. Mus musculus. [Contig view]
GeneID16828.
KEGGmmu:16828.

Organism-specific databases

MGIMGI:96759. Ldha.

Phylogenomic databases

HOGENOMP06151.
HOVERGENP06151.

Enzyme and pathway databases

BRENDA1.1.1.27. 244.

Gene expression databases

ArrayExpressP06151.
BgeeP06151.
CleanExMM_LDHA.
GermOnlineENSMUSG00000063229. Mus musculus.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
TIGRFAMsTIGR01771. L-LDH-NAD. 1 hit.
PROSITEPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio290734.
SOURCESearch...

Entry information

Entry nameLDHA_MOUSE
AccessionPrimary (citable) accession number: P06151
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents