Reviewed, UniProtKB/Swiss-Prot P06151 (LDHA_MOUSE)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase A chain
      Short name=LDH-A
    EC=1.1.1.27
Alternative name(s):
    LDH muscle subunit
      Short name=LDH-M

Gene names

Name:	Ldha
Synonyms:	Ldh-1, Ldh1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: InterPro cellular response to extracellular stimulus Inferred from direct assay. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: MGI flagellum Inferred from direct assay. Source: MGI
Molecular function	L-lactate dehydrogenase activity Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Hnrnpd	Q60668	2	EBI-444940,EBI-299932
Pik3cg	Q9JHG7	2	EBI-444940,EBI-644372

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 332	331	L-lactate dehydrogenase A chain		PRO_0000168414
Regions
	Nucleotide binding	29 – 57	29	NAD By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	99	1	NAD By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	138	1	NAD or substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	239	1	Phosphotyrosine Ref.7 Ref.8
Experimental info
	Sequence conflict	11	1	N → I Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P06151-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5AEB41E1E0B95100	FASTA	332	36,499
	10 20 30 40 50 60 MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG 70 80 90 100 110 120 EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI 130 140 150 160 170 180 IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV 190 200 210 220 230 240 HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE 250 260 270 280 290 300 VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI 310 320 330 SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of the mouse lactate dehydrogenase-A functional gene: comparison of the exon-intron organization of dehydrogenase genes." Fukasawa K.M., Li S.S.-L. Genetics 116:99-105(1987) [PubMed: 3036647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/10. Tissue: Liver.
[2]	"Protein structure and gene organization of mouse lactate dehydrogenase-A isozyme." Li S.S.-L., Tiano H.F., Fukasawa K.M., Yagi K., Shimizu M., Sharief F.S., Nakashima Y., Pan Y.E. Eur. J. Biochem. 149:215-225(1985) [PubMed: 3996406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Lactate dehydrogenase-A mRNAs in mouse testis and somatic tissues containing different 5' noncoding sequences." Hiraoka Y., Li S.S.-L. J. Genet. Mol. Biol. 1:1-6(1990) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA/2J.
[4]	"Nucleotide sequence of the putative regulatory region of mouse lactate dehydrogenase-A gene." Fukasawa K.M., Li S.S.-L. Biochem. J. 235:435-439(1986) [PubMed: 3017306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
[5]	Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 6-14; 23-73; 82-99; 107-126; 133-149; 158-169; 172-177; 213-228; 233-243; 246-265; 269-315 AND 319-328, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
[6]	"Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme." Akai K., Yagi K., Tiano H.F., Pan Y.-C.E., Shimizu M., Fong K., Jungmann R.A., Li S.S.-L. Int. J. Biochem. 17:645-648(1985) [PubMed: 2993055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 202-332.
[7]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, MASS SPECTROMETRY. Tissue: Mast cell.
[8]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00309 Genomic DNA. Translation: CAA68410.1. X02520 , X02521, X02522, X02523, X02524, X02525, X02526 Genomic DNA. Translation: CAA26360.1. U13687 mRNA. Translation: AAA21466.1. X03753 Genomic DNA. Translation: CAA27387.1. M17516 mRNA. Translation: AAA39424.1.
IPI	IPI00319994.
PIR	DEMSLM. A25205. I48240.
RefSeq	NP_001129541.1. NP_034829.1.
UniGene	Mm.26751 Mm.29324
3D structure databases
HSSP	HSSP built from PDB template 1I10 based on UniProtKB P00338.
SMR	P06151. Positions 2-332.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06151. 4 interactions.
PTM databases
PhosphoSite	P06151.
2-D gel databases
SWISS-2DPAGE	P06151.
REPRODUCTION-2DPAGE	P06151.
Proteomic databases
PRIDE	P06151.
Genome annotation databases
Ensembl	ENSMUSG00000063229. Mus musculus. [Contig view]
GeneID	16828.
KEGG	mmu:16828.
Organism-specific databases
MGI	MGI:96759. Ldha.
Phylogenomic databases
HOGENOM	P06151.
HOVERGEN	P06151.
Enzyme and pathway databases
BRENDA	1.1.1.27. 244.
Gene expression databases
ArrayExpress	P06151.
Bgee	P06151.
CleanEx	MM_LDHA.
GermOnline	ENSMUSG00000063229. Mus musculus.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	290734.
SOURCE	Search...

Entry information

Entry name

LDHA_MOUSE

Accession

Primary (citable) accession number: P06151

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 99 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)