L-lactate dehydrogenase A chain - Mus musculus (Mouse)

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P06151 (LDHA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 127. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase A chain
Short name=LDH-A
EC=1.1.1.27

Alternative name(s):
LDH muscle subunit
Short name=LDH-M

Gene names

Name:	Ldha
Synonyms:	Ldh-1, Ldh1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD⁺ = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Post-translational modification	ISGylated. Ref.7
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular response to extracellular stimulus Inferred from direct assay. Source: MGI glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: MGI flagellum Inferred from direct assay. Source: MGI mitochondrion Inferred from direct assay. Source: MGI
Molecular function	L-lactate dehydrogenase activity Inferred from direct assay. Source: MGI nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Hnrnpd	Q60668	2	EBI-444940,EBI-299932

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 332

331

L-lactate dehydrogenase A chain

PRO_0000168414

Regions

Nucleotide binding

29 – 57

NAD By similarity

Sites

Active site

193

Proton acceptor By similarity

Binding site

NAD By similarity

Binding site

106

Substrate By similarity

Binding site

138

NAD or substrate By similarity

Binding site

169

Substrate By similarity

Binding site

248

Substrate By similarity

Amino acid modifications

Modified residue

N-acetylalanine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

118

N6-acetyllysine By similarity

Modified residue

126

N6-acetyllysine By similarity

Modified residue

239

Phosphotyrosine Ref.8 Ref.9

Modified residue

278

N6-acetyllysine By similarity

Modified residue

318

N6-acetyllysine By similarity

Experimental info

Sequence conflict

N → I Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P06151 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 5AEB41E1E0B95100
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FASTA

332

36,499

        10         20         30         40         50         60 
MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG 

        70         80         90        100        110        120 
EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI 

       130        140        150        160        170        180 
IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV 

       190        200        210        220        230        240 
HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE 

       250        260        270        280        290        300 
VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI 

       310        320        330 
SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of the mouse lactate dehydrogenase-A functional gene: comparison of the exon-intron organization of dehydrogenase genes." Fukasawa K.M., Li S.S.-L. Genetics 116:99-105(1987) [PubMed: 3036647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/10. Tissue: Liver.
[2]	"Protein structure and gene organization of mouse lactate dehydrogenase-A isozyme." Li S.S.-L., Tiano H.F., Fukasawa K.M., Yagi K., Shimizu M., Sharief F.S., Nakashima Y., Pan Y.E. Eur. J. Biochem. 149:215-225(1985) [PubMed: 3996406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Lactate dehydrogenase-A mRNAs in mouse testis and somatic tissues containing different 5' noncoding sequences." Hiraoka Y., Li S.S.-L. J. Genet. Mol. Biol. 1:1-6(1990) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA/2J.
[4]	"Nucleotide sequence of the putative regulatory region of mouse lactate dehydrogenase-A gene." Fukasawa K.M., Li S.S.-L. Biochem. J. 235:435-439(1986) [PubMed: 3017306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
[5]	Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 6-14; 23-73; 82-99; 107-126; 133-149; 158-169; 172-177; 213-228; 233-243; 246-265; 269-315 AND 319-328, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
[6]	"Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme." Akai K., Yagi K., Tiano H.F., Pan Y.-C.E., Shimizu M., Fong K., Jungmann R.A., Li S.S.-L. Int. J. Biochem. 17:645-648(1985) [PubMed: 2993055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 202-332.
[7]	"Proteomic identification of proteins conjugated to ISG15 in mouse and human cells." Giannakopoulos N.V., Luo J.K., Papov V., Zou W., Lenschow D.J., Jacobs B.S., Borden E.C., Li J., Virgin H.W., Zhang D.E. Biochem. Biophys. Res. Commun. 336:496-506(2005) [PubMed: 16139798] [Abstract] Cited for: ISGYLATION.
[8]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, MASS SPECTROMETRY. Tissue: Mast cell.
[9]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00309 Genomic DNA. Translation: CAA68410.1. X02520 X02526 Genomic DNA. Translation: CAA26360.1. U13687 mRNA. Translation: AAA21466.1. X03753 Genomic DNA. Translation: CAA27387.1. M17516 mRNA. Translation: AAA39424.1.
IPI	IPI00319994.
PIR	DEMSLM. A25205. I48240.
RefSeq	NP_034829.1. NM_010699.2.
UniGene	Mm.29324.
3D structure databases
ProteinModelPortal	P06151.
SMR	P06151. Positions 2-332.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06151. 9 interactions.
STRING	P06151.
PTM databases
PhosphoSite	P06151.
2D gel databases
SWISS-2DPAGE	P06151.
REPRODUCTION-2DPAGE	P06151.
UCD-2DPAGE	P06151.
Proteomic databases
PRIDE	P06151.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000048209; ENSMUSP00000036386; ENSMUSG00000063229.
GeneID	16828.
KEGG	mmu:16828.
Organism-specific databases
CTD	3939.
MGI	MGI:96759. Ldha.
Phylogenomic databases
eggNOG	roNOG06331.
HOGENOM	HBG566126.
HOVERGEN	HBG000462.
InParanoid	P06151.
OrthoDB	EOG4RR6HR.
Gene expression databases
ArrayExpress	P06151.
Bgee	P06151.
CleanEx	MM_LDHA.
Genevestigator	P06151.
GermOnline	ENSMUSG00000063229. Mus musculus.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00016.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	290734.
SOURCE	Search...

Entry information

Entry name

LDHA_MOUSE

Accession

Primary (citable) accession number: P06151

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 127 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents