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Reviewed, UniProtKB/Swiss-Prot P06149 (DLD_ECOLI)

Last modified June 16, 2009. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    D-lactate dehydrogenase
    EC=1.1.1.28
Alternative name(s):
    Respiratory D-lactate dehydrogenase
Gene names
Name: dld
Ordered Locus Names: b2133, JW2121
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

First component of the membrane-bound D-lactate oxidase, which is believed to play an important role in the energization of the active transport of a variety of sugars and amino acids.

Catalytic activity

(R)-lactate + NAD+ = pyruvate + NADH.

Cofactor

FAD.

Enzyme regulation

Requires phospholipid for maximal activity.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Sequence similarities

Contains 1 FAD-binding PCMH-type domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 571570D-lactate dehydrogenase
PRO_0000079928

Regions

Domain42 – 213172FAD-binding PCMH-type

Secondary structure

1 .................................................................................... 571
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P06149-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 77FB2C467CB4389F

FASTA57164,612
        10         20         30         40         50         60 
MSSMTTTDNK AFLNELARLV GSSHLLTDPA KTARYRKGFR SGQGDALAVV FPGSLLELWR 

        70         80         90        100        110        120 
VLKACVTADK IILMQAANTG LTEGSTPNGN DYDRDVVIIS TLRLDKLHVL GKGEQVLAYP 

       130        140        150        160        170        180 
GTTLYSLEKA LKPLGREPHS VIGSSCIGAS VIGGICNNSG GSLVQRGPAY TEMSLFARIN 

       190        200        210        220        230        240 
EDGKLTLVNH LGIDLGETPE QILSKLDDDR IKDDDVRHDG RHAHDYDYVH RVRDIEADTP 

       250        260        270        280        290        300 
ARYNADPDRL FESSGCAGKL AVFAVRLDTF EAEKNQQVFY IGTNQPEVLT EIRRHILANF 

       310        320        330        340        350        360 
ENLPVAGEYM HRDIYDIAEK YGKDTFLMID KLGTDKMPFF FNLKGRTDAM LEKVKFFRPH 

       370        380        390        400        410        420 
FTDRAMQKFG HLFPSHLPPR MKNWRDKYEH HLLLKMAGDG VGEAKSWLVD YFKQAEGDFF 

       430        440        450        460        470        480 
VCTPEEGSKA FLHRFAAAGA AIRYQAVHSD EVEDILALDI ALRRNDTEWY EHLPPEIDSQ 

       490        500        510        520        530        540 
LVHKLYYGHF MCYVFHQDYI VKKGVDVHAL KEQMLELLQQ RGAQYPAEHN VGHLYKAPET 

       550        560        570 
LQKFYRENDP TNSMNPGIGK TSKRKNWQEV E

« Hide

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References

« Hide 'large scale' references
[1]"Nucleotide sequence of the respiratory D-lactate dehydrogenase gene of Escherichia coli."
Campbell H.D., Rogers B.L., Young I.G.
Eur. J. Biochem. 144:367-373(1984) [PubMed: 6386470] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli."
Rule G.S., Pratt E.A., Chin C.C.Q., Wold F., Ho C.
J. Bacteriol. 161:1059-1068(1985) [PubMed: 3882663] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-18.
[3]"Automated multiplex sequencing of the E.coli genome."
Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / BHB2600.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"The crystal structure of D-lactate dehydrogenase, a peripheral membrane respiratory enzyme."
Dym O., Pratt E.A., Ho C., Eisenberg D.
Proc. Natl. Acad. Sci. U.S.A. 97:9413-9418(2000) [PubMed: 10944213] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

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Cross-references

Sequence databases

M10038 Genomic DNA. Translation: AAA23688.1.
X01067 Genomic DNA. Translation: CAA25531.1.
U00007 Genomic DNA. Translation: AAA60530.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75194.1.
AP009048 Genomic DNA. Translation: BAE76610.1.
PIRDEECDL. A21893.
RefSeqAP_002730.1.
NP_416637.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1F0XX-ray1.90A/B1-571[»]
ModBaseSearch...

2-D gel databases

ECO2DBASEH062.0. 6TH EDITION.

Genome annotation databases

GeneID946653.
GenomeReviewsGene locus JW2121 in contig AP009048_GR.
Gene locus b2133 in contig U00096_GR.
KEGGecj:JW2121.
eco:b2133.

Organism-specific databases

EchoBASEEB0227.
EcoGeneEG10231. dld.
CMRSearch...

Phylogenomic databases

HOGENOMP06149.
OMAP06149. ELVNHLG.

Enzyme and pathway databases

BioCycEcoCyc:DLACTDEHYDROGFAD-MON.
MetaCyc:DLACTDEHYDROGFAD-MON.

Family and domain databases

InterProIPR016172. D-lactate_DH_C-sub1.
IPR016173. D-lactate_DH_C-sub2.
IPR012256. D_lactate_DH.
IPR016166. FAD-bd_2.
IPR016168. FAD-linked_Oxase_FAD-bd_sub2.
IPR015409. Lactate_DH_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
Gene3DG3DSA:3.30.70.610. D-lactate_dehydrogenase_C-sub1. 1 hit.
G3DSA:3.30.1370.20. D-lactate_dehydrogenase_C-sub2. 1 hit.
G3DSA:3.30.465.20. FAD-linked_oxidase_FAD-bd_sub2. 1 hit.
PfamPF01565. FAD_binding_4. 1 hit.
PF09330. Lact-deh-memb. 1 hit.
[Graphical view]
PIRSFPIRSF000101. D-lactate_dh. 1 hit.
PROSITEPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00756. Hexachlorophene.

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Entry information

Entry nameDLD_ECOLI
AccessionPrimary (citable) accession number: P06149
Secondary accession number(s): Q2MAU6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents