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P06133 (UD2B4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glucuronosyltransferase 2B4

Short name=UDPGT 2B4
EC=2.4.1.17
Alternative name(s):
HLUG25
Hyodeoxycholic acid-specific UDPGT
UDPGTh-1
Gene names
Name:UGT2B4
Synonyms:UGT2B11
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid.

Catalytic activity

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

Subcellular location

Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Caution

Ref.2 thought that this was a new form (UGT2B11). The name UGT2B11 has now been reused for another human enzyme.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 528505UDP-glucuronosyltransferase 2B4
PRO_0000036028

Regions

Transmembrane493 – 50917Helical; Potential

Amino acid modifications

Glycosylation3151N-linked (GlcNAc...) Ref.9

Natural variations

Natural variant401K → N. Ref.6
Corresponds to variant rs41299974 [ dbSNP | Ensembl ].
VAR_060713
Natural variant781P → T. Ref.6
Corresponds to variant rs41299976 [ dbSNP | Ensembl ].
VAR_060714
Natural variant801S → P. Ref.6
Corresponds to variant rs41299978 [ dbSNP | Ensembl ].
VAR_060715
Natural variant1091F → L. Ref.4
VAR_011328
Natural variant2771V → I. Ref.6
Corresponds to variant rs41300004 [ dbSNP | Ensembl ].
VAR_060716
Natural variant3961F → L. Ref.4
VAR_011329
Natural variant4581D → E. Ref.3 Ref.6
Corresponds to variant rs13119049 [ dbSNP | Ensembl ].
VAR_007712
Natural variant5111C → R. Ref.6
VAR_060717

Experimental info

Sequence conflict171 – 1722SL → RP in CAA68415. Ref.1
Sequence conflict291 – 2933EME → KWK in AAC32272. Ref.4
Sequence conflict382 – 3876EAIYHG → KAISPR in CAA68415. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P06133 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 6B45E6769971A078

FASTA52860,513
        10         20         30         40         50         60 
MSMKWTSALL LIQLSCYFSS GSCGKVLVWP TEFSHWMNIK TILDELVQRG HEVTVLASSA 

        70         80         90        100        110        120 
SISFDPNSPS TLKFEVYPVS LTKTEFEDII KQLVKRWAEL PKDTFWSYFS QVQEIMWTFN 

       130        140        150        160        170        180 
DILRKFCKDI VSNKKLMKKL QESRFDVVLA DAVFPFGELL AELLKIPFVY SLRFSPGYAI 

       190        200        210        220        230        240 
EKHSGGLLFP PSYVPVVMSE LSDQMTFIER VKNMIYVLYF EFWFQIFDMK KWDQFYSEVL 

       250        260        270        280        290        300 
GRPTTLSETM AKADIWLIRN YWDFQFPHPL LPNVEFVGGL HCKPAKPLPK EMEEFVQSSG 

       310        320        330        340        350        360 
ENGVVVFSLG SMVSNTSEER ANVIASALAK IPQKVLWRFD GNKPDTLGLN TRLYKWIPQN 

       370        380        390        400        410        420 
DLLGHPKTRA FITHGGANGI YEAIYHGIPM VGVPLFADQP DNIAHMKAKG AAVSLDFHTM 

       430        440        450        460        470        480 
SSTDLLNALK TVINDPLYKE NAMKLSRIHH DQPVKPLDRA VFWIEFVMRH KGAKHLRVAA 

       490        500        510        520 
HDLTWFQYHS LDVTGFLLAC VATVIFIITK CLFCVWKFVR TGKKGKRD 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA."
Jackson M.R., McCarthy L.R., Harding D., Wilson S., Coughtrie M.W.H., Burchell B.
Biochem. J. 242:581-588(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"cDNA cloning and expression of two new members of the human liver UDP-glucuronosyltransferase 2B subfamily."
Jin C.-J., Miners J.O., Lillywhite K.J., McKenzie P.I.
Biochem. Biophys. Res. Commun. 194:496-503(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"Characterization and substrate specificity of UGT2B4 (E458): a UDP-glucuronosyltransferase encoded by a polymorphic gene."
Levesque E., Beaulieu M., Hum D.W., Belanger A.
Pharmacogenetics 9:207-216(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-458, CHARACTERIZATION.
[4]McKenzie P.I.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS LEU-109 AND LEU-396.
[5]"Genomic organization and structure of the UGT2B gene complex at human chromosome 4q13."
Riedy M., Miller A.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]NIEHS SNPs program
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASN-40; THR-78; PRO-80; ILE-277; GLU-458 AND ARG-511.
[7]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[9]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-315.
Tissue: Liver.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00317 mRNA. Translation: CAA68415.1.
AF064200 mRNA. Translation: AAC95002.1.
AJ005162 mRNA. Translation: CAA06396.1.
AF081793 mRNA. Translation: AAC32272.1.
AF135416 Genomic DNA. Translation: AAF78145.1.
DQ520733 Genomic DNA. Translation: ABF47107.1.
AK292748 mRNA. Translation: BAF85437.1.
BC026264 mRNA. Translation: AAH26264.1.
PIRJN0619.
RefSeqNP_066962.2. NM_021139.2.
UniGeneHs.285887.

3D structure databases

ProteinModelPortalP06133.
SMRP06133. Positions 285-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113210. 1 interaction.
IntActP06133. 1 interaction.
STRING9606.ENSP00000305221.

Chemistry

ChEMBLCHEMBL6196.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSiteP06133.

Polymorphism databases

DMDM6175083.

Proteomic databases

PaxDbP06133.
PRIDEP06133.

Protocols and materials databases

DNASU7363.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000305107; ENSP00000305221; ENSG00000156096.
GeneID7363.
KEGGhsa:7363.
UCSCuc003hek.4. human.

Organism-specific databases

CTD7363.
GeneCardsGC04M070345.
HGNCHGNC:12553. UGT2B4.
HPACAB033260.
HPA045108.
MIM600067. gene.
neXtProtNX_P06133.
PharmGKBPA360.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1819.
HOGENOMHOG000220831.
HOVERGENHBG004033.
InParanoidP06133.
KOK00699.
OMAKQLVKRW.
PhylomeDBP06133.
TreeFamTF315472.

Enzyme and pathway databases

BRENDA2.4.1.17. 2681.
ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressP06133.
BgeeP06133.
CleanExHS_UGT2B11.
HS_UGT2B4.
GenevestigatorP06133.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUGT2B4. human.
GeneWikiUGT2B4.
GenomeRNAi7363.
NextBio28828.
PROP06133.
SOURCESearch...

Entry information

Entry nameUD2B4_HUMAN
AccessionPrimary (citable) accession number: P06133
Secondary accession number(s): O60731 expand/collapse secondary AC list , O60867, O75614, P36538, Q1HBF9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 15, 1998
Last modified: April 16, 2014
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM