Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P06133

- UD2B4_HUMAN

UniProt

P06133 - UD2B4_HUMAN

Protein

UDP-glucuronosyltransferase 2B4

Gene

UGT2B4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid.

    Catalytic activityi

    UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

    GO - Molecular functioni

    1. glucuronosyltransferase activity Source: BHF-UCL

    GO - Biological processi

    1. cellular glucuronidation Source: BHF-UCL
    2. estrogen catabolic process Source: UniProtKB
    3. metabolic process Source: UniProtKB
    4. xenobiotic metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.17. 2681.
    ReactomeiREACT_6784. Glucuronidation.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucuronosyltransferase 2B4 (EC:2.4.1.17)
    Short name:
    UDPGT 2B4
    Alternative name(s):
    HLUG25
    Hyodeoxycholic acid-specific UDPGT
    UDPGTh-1
    Gene namesi
    Name:UGT2B4
    Synonyms:UGT2B11
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:12553. UGT2B4.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA360.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323By similarityAdd
    BLAST
    Chaini24 – 528505UDP-glucuronosyltransferase 2B4PRO_0000036028Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi315 – 3151N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP06133.
    PaxDbiP06133.
    PRIDEiP06133.

    PTM databases

    PhosphoSiteiP06133.

    Expressioni

    Gene expression databases

    ArrayExpressiP06133.
    BgeeiP06133.
    CleanExiHS_UGT2B11.
    HS_UGT2B4.
    GenevestigatoriP06133.

    Organism-specific databases

    HPAiCAB033260.
    HPA045108.

    Interactioni

    Protein-protein interaction databases

    BioGridi113210. 1 interaction.
    IntActiP06133. 1 interaction.
    STRINGi9606.ENSP00000305221.

    Structurei

    3D structure databases

    ProteinModelPortaliP06133.
    SMRiP06133. Positions 285-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei493 – 50917HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1819.
    HOGENOMiHOG000220831.
    HOVERGENiHBG004033.
    InParanoidiP06133.
    KOiK00699.
    OMAiKQLVKRW.
    PhylomeDBiP06133.
    TreeFamiTF315472.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P06133-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSMKWTSALL LIQLSCYFSS GSCGKVLVWP TEFSHWMNIK TILDELVQRG    50
    HEVTVLASSA SISFDPNSPS TLKFEVYPVS LTKTEFEDII KQLVKRWAEL 100
    PKDTFWSYFS QVQEIMWTFN DILRKFCKDI VSNKKLMKKL QESRFDVVLA 150
    DAVFPFGELL AELLKIPFVY SLRFSPGYAI EKHSGGLLFP PSYVPVVMSE 200
    LSDQMTFIER VKNMIYVLYF EFWFQIFDMK KWDQFYSEVL GRPTTLSETM 250
    AKADIWLIRN YWDFQFPHPL LPNVEFVGGL HCKPAKPLPK EMEEFVQSSG 300
    ENGVVVFSLG SMVSNTSEER ANVIASALAK IPQKVLWRFD GNKPDTLGLN 350
    TRLYKWIPQN DLLGHPKTRA FITHGGANGI YEAIYHGIPM VGVPLFADQP 400
    DNIAHMKAKG AAVSLDFHTM SSTDLLNALK TVINDPLYKE NAMKLSRIHH 450
    DQPVKPLDRA VFWIEFVMRH KGAKHLRVAA HDLTWFQYHS LDVTGFLLAC 500
    VATVIFIITK CLFCVWKFVR TGKKGKRD 528
    Length:528
    Mass (Da):60,513
    Last modified:December 15, 1998 - v2
    Checksum:i6B45E6769971A078
    GO
    Isoform 2 (identifier: P06133-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-14: MSMKWTSALLLIQL → MFFALLHVSSTNGL
         15-150: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:392
    Mass (Da):44,593
    Checksum:i86803DC78D6790A6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti171 – 1722SL → RP in CAA68415. (PubMed:3109396)Curated
    Sequence conflicti291 – 2933EME → KWK in AAC32272. 1 PublicationCurated
    Sequence conflicti382 – 3876EAIYHG → KAISPR in CAA68415. (PubMed:3109396)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti40 – 401K → N.1 Publication
    Corresponds to variant rs41299974 [ dbSNP | Ensembl ].
    VAR_060713
    Natural varianti78 – 781P → T.1 Publication
    Corresponds to variant rs41299976 [ dbSNP | Ensembl ].
    VAR_060714
    Natural varianti80 – 801S → P.1 Publication
    Corresponds to variant rs41299978 [ dbSNP | Ensembl ].
    VAR_060715
    Natural varianti109 – 1091F → L.1 Publication
    VAR_011328
    Natural varianti277 – 2771V → I.1 Publication
    Corresponds to variant rs41300004 [ dbSNP | Ensembl ].
    VAR_060716
    Natural varianti396 – 3961F → L.1 Publication
    VAR_011329
    Natural varianti458 – 4581D → E.2 Publications
    Corresponds to variant rs13119049 [ dbSNP | Ensembl ].
    VAR_007712
    Natural varianti511 – 5111C → R.1 Publication
    VAR_060717

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1414MSMKW…LLIQL → MFFALLHVSSTNGL in isoform 2. 1 PublicationVSP_056679Add
    BLAST
    Alternative sequencei15 – 150136Missing in isoform 2. 1 PublicationVSP_056680Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00317 mRNA. Translation: CAA68415.1.
    AF064200 mRNA. Translation: AAC95002.1.
    AJ005162 mRNA. Translation: CAA06396.1.
    AF081793 mRNA. Translation: AAC32272.1.
    AF135416 Genomic DNA. Translation: AAF78145.1.
    DQ520733 Genomic DNA. Translation: ABF47107.1.
    AK292748 mRNA. Translation: BAF85437.1.
    AK300084 mRNA. Translation: BAG61887.1.
    AC093829 Genomic DNA. No translation available.
    AC108078 Genomic DNA. No translation available.
    BC026264 mRNA. Translation: AAH26264.1.
    CCDSiCCDS43234.1.
    PIRiJN0619.
    RefSeqiNP_066962.2. NM_021139.2.
    UniGeneiHs.285887.

    Genome annotation databases

    EnsembliENST00000305107; ENSP00000305221; ENSG00000156096.
    ENST00000381096; ENSP00000370486; ENSG00000156096.
    GeneIDi7363.
    KEGGihsa:7363.
    UCSCiuc003hek.4. human.

    Polymorphism databases

    DMDMi6175083.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00317 mRNA. Translation: CAA68415.1 .
    AF064200 mRNA. Translation: AAC95002.1 .
    AJ005162 mRNA. Translation: CAA06396.1 .
    AF081793 mRNA. Translation: AAC32272.1 .
    AF135416 Genomic DNA. Translation: AAF78145.1 .
    DQ520733 Genomic DNA. Translation: ABF47107.1 .
    AK292748 mRNA. Translation: BAF85437.1 .
    AK300084 mRNA. Translation: BAG61887.1 .
    AC093829 Genomic DNA. No translation available.
    AC108078 Genomic DNA. No translation available.
    BC026264 mRNA. Translation: AAH26264.1 .
    CCDSi CCDS43234.1.
    PIRi JN0619.
    RefSeqi NP_066962.2. NM_021139.2.
    UniGenei Hs.285887.

    3D structure databases

    ProteinModelPortali P06133.
    SMRi P06133. Positions 285-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113210. 1 interaction.
    IntActi P06133. 1 interaction.
    STRINGi 9606.ENSP00000305221.

    Chemistry

    ChEMBLi CHEMBL6196.

    Protein family/group databases

    CAZyi GT1. Glycosyltransferase Family 1.

    PTM databases

    PhosphoSitei P06133.

    Polymorphism databases

    DMDMi 6175083.

    Proteomic databases

    MaxQBi P06133.
    PaxDbi P06133.
    PRIDEi P06133.

    Protocols and materials databases

    DNASUi 7363.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000305107 ; ENSP00000305221 ; ENSG00000156096 .
    ENST00000381096 ; ENSP00000370486 ; ENSG00000156096 .
    GeneIDi 7363.
    KEGGi hsa:7363.
    UCSCi uc003hek.4. human.

    Organism-specific databases

    CTDi 7363.
    GeneCardsi GC04M070345.
    HGNCi HGNC:12553. UGT2B4.
    HPAi CAB033260.
    HPA045108.
    MIMi 600067. gene.
    neXtProti NX_P06133.
    PharmGKBi PA360.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1819.
    HOGENOMi HOG000220831.
    HOVERGENi HBG004033.
    InParanoidi P06133.
    KOi K00699.
    OMAi KQLVKRW.
    PhylomeDBi P06133.
    TreeFami TF315472.

    Enzyme and pathway databases

    BRENDAi 2.4.1.17. 2681.
    Reactomei REACT_6784. Glucuronidation.

    Miscellaneous databases

    ChiTaRSi UGT2B4. human.
    GeneWikii UGT2B4.
    GenomeRNAii 7363.
    NextBioi 28828.
    PROi P06133.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P06133.
    Bgeei P06133.
    CleanExi HS_UGT2B11.
    HS_UGT2B4.
    Genevestigatori P06133.

    Family and domain databases

    InterProi IPR002213. UDP_glucos_trans.
    [Graphical view ]
    PANTHERi PTHR11926. PTHR11926. 1 hit.
    Pfami PF00201. UDPGT. 1 hit.
    [Graphical view ]
    PROSITEi PS00375. UDPGT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA."
      Jackson M.R., McCarthy L.R., Harding D., Wilson S., Coughtrie M.W.H., Burchell B.
      Biochem. J. 242:581-588(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Liver.
    2. "cDNA cloning and expression of two new members of the human liver UDP-glucuronosyltransferase 2B subfamily."
      Jin C.-J., Miners J.O., Lillywhite K.J., McKenzie P.I.
      Biochem. Biophys. Res. Commun. 194:496-503(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Liver.
    3. "Characterization and substrate specificity of UGT2B4 (E458): a UDP-glucuronosyltransferase encoded by a polymorphic gene."
      Levesque E., Beaulieu M., Hum D.W., Belanger A.
      Pharmacogenetics 9:207-216(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLU-458, CHARACTERIZATION.
    4. McKenzie P.I.
      Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS LEU-109 AND LEU-396.
    5. "Genomic organization and structure of the UGT2B gene complex at human chromosome 4q13."
      Riedy M., Miller A.
      Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. NIEHS SNPs program
      Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASN-40; THR-78; PRO-80; ILE-277; GLU-458 AND ARG-511.
    7. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Liver and Pericardium.
    8. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Liver.
    10. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-315.
      Tissue: Liver.

    Entry informationi

    Entry nameiUD2B4_HUMAN
    AccessioniPrimary (citable) accession number: P06133
    Secondary accession number(s): A6NCP7
    , B4DT75, O60731, O60867, O75614, P36538, Q1HBF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 149 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3