P06133 (UD2B4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucuronosyltransferase 2B4
Short name=UDPGT 2B4
EC=2.4.1.17

Alternative name(s):
HLUG25
Hyodeoxycholic acid-specific UDPGT
UDPGTh-1

Gene names

Name:	UGT2B4
Synonyms:	UGT2B11

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid.
Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.
Subcellular location	Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
Caution	Ref.2 thought that this was a new form (UGT2B11). The name UGT2B11 has now been reused for another human enzyme.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular glucuronidation Inferred from direct assay. Source: BHF-UCL estrogen catabolic process Inferred from direct assay Ref.2. Source: UniProtKB xenobiotic metabolic process Inferred from direct assay Ref.2. Source: UniProtKB
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Non-traceable author statement. Source: UniProtKB
Molecular function	glucuronosyltransferase activity Inferred from direct assay. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 528	505	UDP-glucuronosyltransferase 2B4		PRO_0000036028
Regions
	Transmembrane	493 – 509	17	Helical; Potential
Amino acid modifications
	Glycosylation	315	1	N-linked (GlcNAc...) Ref.9
Natural variations
	Natural variant	40	1	K → N. Ref.6		VAR_060713
	Natural variant	78	1	P → T. Ref.6		VAR_060714
	Natural variant	80	1	S → P. Ref.6		VAR_060715
	Natural variant	109	1	F → L. Ref.4		VAR_011328
	Natural variant	277	1	V → I. Ref.6		VAR_060716
	Natural variant	396	1	F → L. Ref.4		VAR_011329
	Natural variant	458	1	D → E. Ref.3 Ref.6 Corresponds to variant rs13119049 [ dbSNP | Ensembl ].		VAR_007712
	Natural variant	511	1	C → R. Ref.6		VAR_060717
Experimental info
	Sequence conflict	171 – 172	2	SL → RP in CAA68415. Ref.1
	Sequence conflict	291 – 293	3	EME → KWK in AAC32272. Ref.4
	Sequence conflict	382 – 387	6	EAIYHG → KAISPR in CAA68415. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P06133 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 6B45E6769971A078	FASTA	528	60,513
	10 20 30 40 50 60 MSMKWTSALL LIQLSCYFSS GSCGKVLVWP TEFSHWMNIK TILDELVQRG HEVTVLASSA 70 80 90 100 110 120 SISFDPNSPS TLKFEVYPVS LTKTEFEDII KQLVKRWAEL PKDTFWSYFS QVQEIMWTFN 130 140 150 160 170 180 DILRKFCKDI VSNKKLMKKL QESRFDVVLA DAVFPFGELL AELLKIPFVY SLRFSPGYAI 190 200 210 220 230 240 EKHSGGLLFP PSYVPVVMSE LSDQMTFIER VKNMIYVLYF EFWFQIFDMK KWDQFYSEVL 250 260 270 280 290 300 GRPTTLSETM AKADIWLIRN YWDFQFPHPL LPNVEFVGGL HCKPAKPLPK EMEEFVQSSG 310 320 330 340 350 360 ENGVVVFSLG SMVSNTSEER ANVIASALAK IPQKVLWRFD GNKPDTLGLN TRLYKWIPQN 370 380 390 400 410 420 DLLGHPKTRA FITHGGANGI YEAIYHGIPM VGVPLFADQP DNIAHMKAKG AAVSLDFHTM 430 440 450 460 470 480 SSTDLLNALK TVINDPLYKE NAMKLSRIHH DQPVKPLDRA VFWIEFVMRH KGAKHLRVAA 490 500 510 520 HDLTWFQYHS LDVTGFLLAC VATVIFIITK CLFCVWKFVR TGKKGKRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA." Jackson M.R., McCarthy L.R., Harding D., Wilson S., Coughtrie M.W.H., Burchell B. Biochem. J. 242:581-588(1987) [PubMed: 3109396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"cDNA cloning and expression of two new members of the human liver UDP-glucuronosyltransferase 2B subfamily." Jin C.-J., Miners J.O., Lillywhite K.J., McKenzie P.I. Biochem. Biophys. Res. Commun. 194:496-503(1993) [PubMed: 8333863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Characterization and substrate specificity of UGT2B4 (E458): a UDP-glucuronosyltransferase encoded by a polymorphic gene." Levesque E., Beaulieu M., Hum D.W., Belanger A. Pharmacogenetics 9:207-216(1999) [PubMed: 10376768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-458, CHARACTERIZATION.
[4]	McKenzie P.I. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS LEU-109 AND LEU-396.
[5]	"Genomic organization and structure of the UGT2B gene complex at human chromosome 4q13." Riedy M., Miller A. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	NIEHS SNPs program Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASN-40; THR-78; PRO-80; ILE-277; GLU-458 AND ARG-511.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[9]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-315, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00317 mRNA. Translation: CAA68415.1. AF064200 mRNA. Translation: AAC95002.1. AJ005162 mRNA. Translation: CAA06396.1. AF081793 mRNA. Translation: AAC32272.1. AF135416 Genomic DNA. Translation: AAF78145.1. DQ520733 Genomic DNA. Translation: ABF47107.1. AK292748 mRNA. Translation: BAF85437.1. BC026264 mRNA. Translation: AAH26264.1.
IPI	IPI00301491.
PIR	JN0619.
RefSeq	NP_066962.2. NM_021139.2.
UniGene	Hs.285887.
3D structure databases
ProteinModelPortal	P06133.
SMR	P06133. Positions 285-446.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06133. 1 interaction.
STRING	P06133.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
PTM databases
PhosphoSite	P06133.
Polymorphism databases
DMDM	6175083.
Proteomic databases
PRIDE	P06133.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000305107; ENSP00000305221; ENSG00000156096.
GeneID	7363.
KEGG	hsa:7363.
UCSC	uc003hek.2. human.
Organism-specific databases
CTD	7363.
GeneCards	GC04M070345.
H-InvDB	HIX0004257.
HGNC	HGNC:12553. UGT2B4.
HPA	CAB033260. HPA045108.
MIM	600067. gene.
neXtProt	NX_P06133.
PharmGKB	PA360.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00560000076681.
HOGENOM	HBG446523.
HOVERGEN	HBG004033.
InParanoid	P06133.
OMA	AIEKHSG.
OrthoDB	EOG4SJ5DW.
PhylomeDB	P06133.
Enzyme and pathway databases
BRENDA	2.4.1.17. 2681.
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	P06133.
Bgee	P06133.
CleanEx	HS_UGT2B11. HS_UGT2B4.
Genevestigator	P06133.
GermOnline	ENSG00000156096. Homo sapiens.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
KO	K00699.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	28828.
SOURCE	Search...

Entry information

Entry name

UD2B4_HUMAN

Accession

Primary (citable) accession number: P06133
Secondary accession number(s): O60731 , O60867, O75614, P36538, Q1HBF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	December 15, 1998
Last modified:	January 25, 2012
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.