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Protein

UDP-glucuronosyltransferase 2B4

Gene

UGT2B4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid.

Catalytic activityi

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

GO - Molecular functioni

  • glucuronosyltransferase activity Source: BHF-UCL

GO - Biological processi

  • cellular glucuronidation Source: BHF-UCL
  • estrogen catabolic process Source: UniProtKB
  • flavonoid biosynthetic process Source: GO_Central
  • flavonoid glucuronidation Source: GO_Central
  • metabolic process Source: UniProtKB
  • xenobiotic metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciZFISH:HS08094-MONOMER.
BRENDAi2.4.1.17. 2681.
ReactomeiR-HSA-156588. Glucuronidation.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucuronosyltransferase 2B4 (EC:2.4.1.17)
Short name:
UDPGT 2B4
Alternative name(s):
HLUG25
Hyodeoxycholic acid-specific UDPGT
UDPGTh-1
Gene namesi
Name:UGT2B4
Synonyms:UGT2B11
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:12553. UGT2B4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei493 – 509HelicalSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

DisGeNETi7363.
OpenTargetsiENSG00000156096.
PharmGKBiPA360.

Chemistry databases

ChEMBLiCHEMBL6196.
DrugBankiDB08907. Canagliflozin.
DB00318. Codeine.
DB06292. Dapagliflozin.
DB00712. Flurbiprofen.
DB01050. Ibuprofen.
DB00295. Morphine.

Polymorphism and mutation databases

BioMutaiUGT2B4.
DMDMi6175083.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23By similarityAdd BLAST23
ChainiPRO_000003602824 – 528UDP-glucuronosyltransferase 2B4Add BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi315N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP06133.
PaxDbiP06133.
PeptideAtlasiP06133.
PRIDEiP06133.

PTM databases

iPTMnetiP06133.
PhosphoSitePlusiP06133.

Expressioni

Gene expression databases

BgeeiENSG00000156096.
CleanExiHS_UGT2B11.
HS_UGT2B4.
ExpressionAtlasiP06133. baseline and differential.
GenevisibleiP06133. HS.

Organism-specific databases

HPAiCAB033260.
HPA045108.

Interactioni

Protein-protein interaction databases

BioGridi113210. 1 interactor.
IntActiP06133. 1 interactor.
STRINGi9606.ENSP00000305221.

Structurei

3D structure databases

ProteinModelPortaliP06133.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG004033.
InParanoidiP06133.
KOiK00699.
OMAiWAELPKD.
OrthoDBiEOG091G06JC.
PhylomeDBiP06133.
TreeFamiTF315472.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P06133-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSMKWTSALL LIQLSCYFSS GSCGKVLVWP TEFSHWMNIK TILDELVQRG
60 70 80 90 100
HEVTVLASSA SISFDPNSPS TLKFEVYPVS LTKTEFEDII KQLVKRWAEL
110 120 130 140 150
PKDTFWSYFS QVQEIMWTFN DILRKFCKDI VSNKKLMKKL QESRFDVVLA
160 170 180 190 200
DAVFPFGELL AELLKIPFVY SLRFSPGYAI EKHSGGLLFP PSYVPVVMSE
210 220 230 240 250
LSDQMTFIER VKNMIYVLYF EFWFQIFDMK KWDQFYSEVL GRPTTLSETM
260 270 280 290 300
AKADIWLIRN YWDFQFPHPL LPNVEFVGGL HCKPAKPLPK EMEEFVQSSG
310 320 330 340 350
ENGVVVFSLG SMVSNTSEER ANVIASALAK IPQKVLWRFD GNKPDTLGLN
360 370 380 390 400
TRLYKWIPQN DLLGHPKTRA FITHGGANGI YEAIYHGIPM VGVPLFADQP
410 420 430 440 450
DNIAHMKAKG AAVSLDFHTM SSTDLLNALK TVINDPLYKE NAMKLSRIHH
460 470 480 490 500
DQPVKPLDRA VFWIEFVMRH KGAKHLRVAA HDLTWFQYHS LDVTGFLLAC
510 520
VATVIFIITK CLFCVWKFVR TGKKGKRD
Length:528
Mass (Da):60,513
Last modified:December 15, 1998 - v2
Checksum:i6B45E6769971A078
GO
Isoform 2 (identifier: P06133-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MSMKWTSALLLIQL → MFFALLHVSSTNGL
     15-150: Missing.

Note: No experimental confirmation available.
Show »
Length:392
Mass (Da):44,593
Checksum:i86803DC78D6790A6
GO
Isoform 3 (identifier: P06133-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     364-369: GHPKTR → DIKRML
     370-528: Missing.

Note: No experimental confirmation available.
Show »
Length:369
Mass (Da):42,723
Checksum:i0590B6A6B4D29FCE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171 – 172SL → RP in CAA68415 (PubMed:3109396).Curated2
Sequence conflicti291 – 293EME → KWK in AAC32272 (Ref. 4) Curated3
Sequence conflicti309L → V in AAS47487 (Ref. 6) 1 Publication1
Sequence conflicti354Y → H in AAS47487 (Ref. 6) 1 Publication1
Sequence conflicti382 – 387EAIYHG → KAISPR in CAA68415 (PubMed:3109396).Curated6

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06071340K → N.1 PublicationCorresponds to variant rs41299974dbSNPEnsembl.1
Natural variantiVAR_06071478P → T.1 PublicationCorresponds to variant rs41299976dbSNPEnsembl.1
Natural variantiVAR_06071580S → P.1 PublicationCorresponds to variant rs41299978dbSNPEnsembl.1
Natural variantiVAR_011328109F → L.1 Publication1
Natural variantiVAR_060716277V → I.1 PublicationCorresponds to variant rs41300004dbSNPEnsembl.1
Natural variantiVAR_011329396F → L.1 PublicationCorresponds to variant rs72552707dbSNPEnsembl.1
Natural variantiVAR_007712458D → E.2 PublicationsCorresponds to variant rs13119049dbSNPEnsembl.1
Natural variantiVAR_060717511C → R.1 PublicationCorresponds to variant rs41298245dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0566791 – 14MSMKW…LLIQL → MFFALLHVSSTNGL in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_05668015 – 150Missing in isoform 2. 1 PublicationAdd BLAST136
Alternative sequenceiVSP_056869364 – 369GHPKTR → DIKRML in isoform 3. 1 Publication6
Alternative sequenceiVSP_056870370 – 528Missing in isoform 3. 1 PublicationAdd BLAST159

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00317 mRNA. Translation: CAA68415.1.
AF064200 mRNA. Translation: AAC95002.1.
AJ005162 mRNA. Translation: CAA06396.1.
AF081793 mRNA. Translation: AAC32272.1.
AF135416 Genomic DNA. Translation: AAF78145.1.
AY529122 mRNA. Translation: AAS47487.1.
DQ520733 Genomic DNA. Translation: ABF47107.1.
AK292748 mRNA. Translation: BAF85437.1.
AK300084 mRNA. Translation: BAG61887.1.
AC093829 Genomic DNA. No translation available.
AC108078 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05591.1.
BC026264 mRNA. Translation: AAH26264.1.
CCDSiCCDS43234.1. [P06133-1]
CCDS75137.1. [P06133-3]
PIRiJN0619.
RefSeqiNP_001284544.1. NM_001297615.1. [P06133-3]
NP_001284545.1. NM_001297616.1. [P06133-2]
NP_066962.2. NM_021139.2. [P06133-1]
UniGeneiHs.285887.

Genome annotation databases

EnsembliENST00000305107; ENSP00000305221; ENSG00000156096. [P06133-1]
ENST00000512583; ENSP00000421290; ENSG00000156096. [P06133-3]
GeneIDi7363.
KEGGihsa:7363.
UCSCiuc003hek.4. human. [P06133-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00317 mRNA. Translation: CAA68415.1.
AF064200 mRNA. Translation: AAC95002.1.
AJ005162 mRNA. Translation: CAA06396.1.
AF081793 mRNA. Translation: AAC32272.1.
AF135416 Genomic DNA. Translation: AAF78145.1.
AY529122 mRNA. Translation: AAS47487.1.
DQ520733 Genomic DNA. Translation: ABF47107.1.
AK292748 mRNA. Translation: BAF85437.1.
AK300084 mRNA. Translation: BAG61887.1.
AC093829 Genomic DNA. No translation available.
AC108078 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05591.1.
BC026264 mRNA. Translation: AAH26264.1.
CCDSiCCDS43234.1. [P06133-1]
CCDS75137.1. [P06133-3]
PIRiJN0619.
RefSeqiNP_001284544.1. NM_001297615.1. [P06133-3]
NP_001284545.1. NM_001297616.1. [P06133-2]
NP_066962.2. NM_021139.2. [P06133-1]
UniGeneiHs.285887.

3D structure databases

ProteinModelPortaliP06133.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113210. 1 interactor.
IntActiP06133. 1 interactor.
STRINGi9606.ENSP00000305221.

Chemistry databases

ChEMBLiCHEMBL6196.
DrugBankiDB08907. Canagliflozin.
DB00318. Codeine.
DB06292. Dapagliflozin.
DB00712. Flurbiprofen.
DB01050. Ibuprofen.
DB00295. Morphine.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

PTM databases

iPTMnetiP06133.
PhosphoSitePlusiP06133.

Polymorphism and mutation databases

BioMutaiUGT2B4.
DMDMi6175083.

Proteomic databases

MaxQBiP06133.
PaxDbiP06133.
PeptideAtlasiP06133.
PRIDEiP06133.

Protocols and materials databases

DNASUi7363.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000305107; ENSP00000305221; ENSG00000156096. [P06133-1]
ENST00000512583; ENSP00000421290; ENSG00000156096. [P06133-3]
GeneIDi7363.
KEGGihsa:7363.
UCSCiuc003hek.4. human. [P06133-1]

Organism-specific databases

CTDi7363.
DisGeNETi7363.
GeneCardsiUGT2B4.
HGNCiHGNC:12553. UGT2B4.
HPAiCAB033260.
HPA045108.
MIMi600067. gene.
neXtProtiNX_P06133.
OpenTargetsiENSG00000156096.
PharmGKBiPA360.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG004033.
InParanoidiP06133.
KOiK00699.
OMAiWAELPKD.
OrthoDBiEOG091G06JC.
PhylomeDBiP06133.
TreeFamiTF315472.

Enzyme and pathway databases

BioCyciZFISH:HS08094-MONOMER.
BRENDAi2.4.1.17. 2681.
ReactomeiR-HSA-156588. Glucuronidation.

Miscellaneous databases

ChiTaRSiUGT2B4. human.
GeneWikiiUGT2B4.
GenomeRNAii7363.
PROiP06133.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000156096.
CleanExiHS_UGT2B11.
HS_UGT2B4.
ExpressionAtlasiP06133. baseline and differential.
GenevisibleiP06133. HS.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUD2B4_HUMAN
AccessioniPrimary (citable) accession number: P06133
Secondary accession number(s): A6NCP7
, B4DT75, G5E9X8, O60731, O60867, O75614, P36538, Q1HBF9, Q6QQX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

PubMed:8333863 thought that this was a new form (UGT2B11). The name UGT2B11 has now been reused for another human enzyme.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.