Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P06133 (UD2B4_HUMAN)

Last modified July 7, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    UDP-glucuronosyltransferase 2B4
      Short name=UDPGT 2B4
    EC=2.4.1.17
Alternative name(s):
    Hyodeoxycholic acid
    HLUG25
    UDPGTh-1
Gene names
Name: UGT2B4
Synonyms: UGT2B11
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid.

Catalytic activity

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

Subcellular location

Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Caution

Ref.2 thought that this was a new form (UGT2B11). The name UGT2B11 has now been reused for another human enzyme.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
   Molecular functionGlycosyltransferase
Transferase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processestrogen catabolic process Ref.2

Inferred from direct assay. Source: UniProtKB

xenobiotic metabolic process Ref.2

Inferred from direct assay. Source: UniProtKB

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

microsome

Non-traceable author statement. Source: UniProtKB

   Molecular functionglucuronosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 528505UDP-glucuronosyltransferase 2B4
PRO_0000036028

Regions

Transmembrane493 – 50917 Potential

Amino acid modifications

Glycosylation3151N-linked (GlcNAc...)

Natural variations

Natural variant1091F → L Ref.4
VAR_011328
Natural variant3961F → L Ref.4
VAR_011329
Natural variant4581D → E: dbSNP rs13119049. Ref.3
VAR_007712

Experimental info

Sequence conflict171 – 1722SL → RP in CAA68415. Ref.1
Sequence conflict291 – 2933EME → KWK in AAC32272. Ref.4
Sequence conflict382 – 3876EAIYHG → KAISPR in CAA68415. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P06133-1 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 6B45E6769971A078

FASTA52860,513
        10         20         30         40         50         60 
MSMKWTSALL LIQLSCYFSS GSCGKVLVWP TEFSHWMNIK TILDELVQRG HEVTVLASSA 

        70         80         90        100        110        120 
SISFDPNSPS TLKFEVYPVS LTKTEFEDII KQLVKRWAEL PKDTFWSYFS QVQEIMWTFN 

       130        140        150        160        170        180 
DILRKFCKDI VSNKKLMKKL QESRFDVVLA DAVFPFGELL AELLKIPFVY SLRFSPGYAI 

       190        200        210        220        230        240 
EKHSGGLLFP PSYVPVVMSE LSDQMTFIER VKNMIYVLYF EFWFQIFDMK KWDQFYSEVL 

       250        260        270        280        290        300 
GRPTTLSETM AKADIWLIRN YWDFQFPHPL LPNVEFVGGL HCKPAKPLPK EMEEFVQSSG 

       310        320        330        340        350        360 
ENGVVVFSLG SMVSNTSEER ANVIASALAK IPQKVLWRFD GNKPDTLGLN TRLYKWIPQN 

       370        380        390        400        410        420 
DLLGHPKTRA FITHGGANGI YEAIYHGIPM VGVPLFADQP DNIAHMKAKG AAVSLDFHTM 

       430        440        450        460        470        480 
SSTDLLNALK TVINDPLYKE NAMKLSRIHH DQPVKPLDRA VFWIEFVMRH KGAKHLRVAA 

       490        500        510        520 
HDLTWFQYHS LDVTGFLLAC VATVIFIITK CLFCVWKFVR TGKKGKRD 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA."
Jackson M.R., McCarthy L.R., Harding D., Wilson S., Coughtrie M.W.H., Burchell B.
Biochem. J. 242:581-588(1987) [PubMed: 3109396] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"cDNA cloning and expression of two new members of the human liver UDP-glucuronosyltransferase 2B subfamily."
Jin C.-J., Miners J.O., Lillywhite K.J., McKenzie P.I.
Biochem. Biophys. Res. Commun. 194:496-503(1993) [PubMed: 8333863] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"Characterization and substrate specificity of UGT2B4 (E458): a UDP-glucuronosyltransferase encoded by a polymorphic gene."
Levesque E., Beaulieu M., Hum D.W., Belanger A.
Pharmacogenetics 9:207-216(1999) [PubMed: 10376768] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-458, CHARACTERIZATION.
[4]McKenzie P.I.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS LEU-109 AND LEU-396.
[5]"Genomic organization and structure of the UGT2B gene complex at human chromosome 4q13."
Riedy M., Miller A.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[7]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-315, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

Y00317 mRNA. Translation: CAA68415.1.
AF064200 mRNA. Translation: AAC95002.1.
AJ005162 mRNA. Translation: CAA06396.1.
AF081793 mRNA. Translation: AAC32272.1.
AF135416 Genomic DNA. Translation: AAF78145.1.
BC026264 mRNA. Translation: AAH26264.1.
IPIIPI00301491.
PIRJN0619.
RefSeqNP_066962.2.
UniGeneHs.285887

3D structure databases

SMRP06133. Positions 285-446.
ModBaseSearch...

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

Proteomic databases

PRIDEP06133.

Genome annotation databases

EnsemblENSG00000156096. Homo sapiens. [Contig view]
GeneID7363.
KEGGhsa:7363.
UCSCuc003hek.2. human.

Organism-specific databases

GeneCardsGC04M070394.
H-InvDBHIX0004257.
HGNCHGNC:12553. UGT2B4.
MIM600067. gene.
PharmGKBPA360.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP06133.
HOVERGENP06133.
OMAP06133. AIEKHSG.

Enzyme and pathway databases

BRENDA2.4.1.17. 247.
ReactomeREACT_13433. Biological oxidations.

Gene expression databases

ArrayExpressP06133.
BgeeP06133.
CleanExHS_UGT2B11.
HS_UGT2B4.
GermOnlineENSG00000156096. Homo sapiens.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. UDP_glucos_trans. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio28828.
SOURCESearch...

Entry information

Entry nameUD2B4_HUMAN
AccessionPrimary (citable) accession number: P06133
Secondary accession number(s): O60731 expand/collapse secondary AC list , O60867, O75614, P36538
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 15, 1998
Last modified: July 7, 2009
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents