Reviewed, UniProtKB/Swiss-Prot P06131 (FDHA_METFO)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase subunit alpha
    EC=1.2.1.2

Gene names

Name:

fdhA

Organism

Methanobacterium formicicum

Taxonomic identifier

2162 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobacterium

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of formate.
Catalytic activity	Formate + NAD+ = CO2 + NADH.
Cofactor	Molybdenum (molybdopterin). Zinc. FAD. Binds 1 4Fe-4S cluster Potential.
Subunit structure	Dimer of an alpha and a beta subunit.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Ligand	4Fe-4S FAD Flavoprotein Iron Iron-sulfur Metal-binding Molybdenum NAD Zinc
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	formate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	formate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 684	684	Formate dehydrogenase subunit alpha		PRO_0000063225
Sites
	Metal binding	10	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	13	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	17	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	45	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06131-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 38D37CF86BFF06E2	FASTA	684	75,717
	10 20 30 40 50 60 MDIKYVPTIC PYCGVGCGMN LVVKDEKVVG VEPWKRHPVN EGKLCPKGNF CYEIIHREDR 70 80 90 100 110 120 LTTPLIKENG EFREATWDEA YDLIASKLGA YDPNEIGFFC CARSPNENIY VNQKFARIVV 130 140 150 160 170 180 GTHNIDHCAR LCHGPTVAGL AASFGSGAMT NSYASFEDAD LIFSIGANSL EAHPLVGRKL 190 200 210 220 230 240 MRAKMNGAYF IVADPRYTPT AKQADQYIPF KTGTDVALMN AMMNVIISEG LEDKEFIEKR 250 260 270 280 290 300 TKNYEELKEV VSKYTPEMAE EITQVPADVI RDIAIKYAKA DKAAIVYSLG ITEHSHGVDN 310 320 330 340 350 360 VMQTANLAML TGNIGRLGTG VNPLRGQNNV QGACDMGALP TDYPGYRKVA DQEVMEDVTC 370 380 390 400 410 420 TWGCSDLGCE PGLKIPEMID AAAKGDLKVL YITGEDPVIS DPDTHHVEEA LNNLDFFVVQ 430 440 450 460 470 480 DIFMTDTAEF ADVVLPAACW AEQEGTFTNG ERRVQLIRKA VDAPGESKYD WEIFCDLAKK 490 500 510 520 530 540 MGADPEMFTY ESAQDIFEEV RTVTPQYAGM NRERLDRPEA LHWPCPSEDH PGTAMMHIEK 550 560 570 580 590 600 FAHPDGLGIF MPLEEQGPME TPDDEYPLIL TTTRLLFHYH AAMTRRAATL DREVPTGYVE 610 620 630 640 650 660 INTEDAAELG IANKEKVKVK SRRGEIEIAA RVTDDIVKGI VNIPMHFREC SANILTNAAA 670 680 IDPKSGMPEY KACAVAISKM EGSK

References

[1]	"Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum." Shuber A.P., Orr E.C., Recny M.A., Schendel P.F., May H.D., Schauer N.L., Ferry J.G. J. Biol. Chem. 261:12942-12947(1986) [PubMed: 3531194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-26. Strain: JF-1.
[2]	"Identification of formate dehydrogenase-specific mRNA species and nucleotide sequence of the fdhC gene of Methanobacterium formicicum." White W.B., Ferry J.G. J. Bacteriol. 174:4997-5004(1992) [PubMed: 1378430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20. Strain: JF-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02581 Genomic DNA. Translation: AAA72182.1.
PIR	A24698.
3D structure databases
HSSP	HSSP built from PDB template 1AA6 based on UniProtKB P07658.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.2. 7357.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006478. Formate_DH_asu. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01591. Fdh-alpha. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDHA_METFO

Accession

Primary (citable) accession number: P06131

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)