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P06131

- FDHA_METFO

UniProt

P06131 - FDHA_METFO

Protein

Formate dehydrogenase subunit alpha

Gene

fdhA

Organism
Methanobacterium formicicum
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of formate.

    Catalytic activityi

    Formate + NAD+ = CO2 + NADH.

    Cofactori

    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity
    Zinc.
    FAD.
    Binds 1 4Fe-4S cluster.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi10 – 101Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi13 – 131Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi17 – 171Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi45 – 451Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: InterPro
    3. formate dehydrogenase (coenzyme F420) activity Source: MENGO
    4. formate dehydrogenase (NAD+) activity Source: UniProtKB-EC
    5. molybdenum ion binding Source: InterPro

    GO - Biological processi

    1. formate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD, Zinc

    Enzyme and pathway databases

    SABIO-RKP06131.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Formate dehydrogenase subunit alpha (EC:1.2.1.2)
    Gene namesi
    Name:fdhA
    OrganismiMethanobacterium formicicum
    Taxonomic identifieri2162 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanobacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 684684Formate dehydrogenase subunit alphaPRO_0000063225Add
    BLAST

    Interactioni

    Subunit structurei

    Dimer of an alpha and a beta subunit.

    Structurei

    3D structure databases

    ProteinModelPortaliP06131.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 59574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006478. Formate_DH_asu.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01591. Fdh-alpha. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P06131-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIKYVPTIC PYCGVGCGMN LVVKDEKVVG VEPWKRHPVN EGKLCPKGNF    50
    CYEIIHREDR LTTPLIKENG EFREATWDEA YDLIASKLGA YDPNEIGFFC 100
    CARSPNENIY VNQKFARIVV GTHNIDHCAR LCHGPTVAGL AASFGSGAMT 150
    NSYASFEDAD LIFSIGANSL EAHPLVGRKL MRAKMNGAYF IVADPRYTPT 200
    AKQADQYIPF KTGTDVALMN AMMNVIISEG LEDKEFIEKR TKNYEELKEV 250
    VSKYTPEMAE EITQVPADVI RDIAIKYAKA DKAAIVYSLG ITEHSHGVDN 300
    VMQTANLAML TGNIGRLGTG VNPLRGQNNV QGACDMGALP TDYPGYRKVA 350
    DQEVMEDVTC TWGCSDLGCE PGLKIPEMID AAAKGDLKVL YITGEDPVIS 400
    DPDTHHVEEA LNNLDFFVVQ DIFMTDTAEF ADVVLPAACW AEQEGTFTNG 450
    ERRVQLIRKA VDAPGESKYD WEIFCDLAKK MGADPEMFTY ESAQDIFEEV 500
    RTVTPQYAGM NRERLDRPEA LHWPCPSEDH PGTAMMHIEK FAHPDGLGIF 550
    MPLEEQGPME TPDDEYPLIL TTTRLLFHYH AAMTRRAATL DREVPTGYVE 600
    INTEDAAELG IANKEKVKVK SRRGEIEIAA RVTDDIVKGI VNIPMHFREC 650
    SANILTNAAA IDPKSGMPEY KACAVAISKM EGSK 684
    Length:684
    Mass (Da):75,717
    Last modified:January 1, 1988 - v1
    Checksum:i38D37CF86BFF06E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02581 Genomic DNA. Translation: AAA72182.1.
    PIRiA24698.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02581 Genomic DNA. Translation: AAA72182.1 .
    PIRi A24698.

    3D structure databases

    ProteinModelPortali P06131.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P06131.

    Family and domain databases

    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006478. Formate_DH_asu.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01591. Fdh-alpha. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum."
      Shuber A.P., Orr E.C., Recny M.A., Schendel P.F., May H.D., Schauer N.L., Ferry J.G.
      J. Biol. Chem. 261:12942-12947(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-26.
      Strain: JF-1.
    2. "Identification of formate dehydrogenase-specific mRNA species and nucleotide sequence of the fdhC gene of Methanobacterium formicicum."
      White W.B., Ferry J.G.
      J. Bacteriol. 174:4997-5004(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
      Strain: JF-1.

    Entry informationi

    Entry nameiFDHA_METFO
    AccessioniPrimary (citable) accession number: P06131
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3