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P06131

- FDHA_METFO

UniProt

P06131 - FDHA_METFO

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Protein

Formate dehydrogenase subunit alpha

Gene

fdhA

Organism
Methanobacterium formicicum
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of formate.

Catalytic activityi

Formate + NAD+ = CO2 + NADH.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi13 – 131Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi17 – 171Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi45 – 451Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. formate dehydrogenase (coenzyme F420) activity Source: MENGO
  4. formate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  5. molybdenum ion binding Source: InterPro

GO - Biological processi

  1. formate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD, Zinc

Enzyme and pathway databases

SABIO-RKP06131.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase subunit alpha (EC:1.2.1.2)
Gene namesi
Name:fdhA
OrganismiMethanobacterium formicicum
Taxonomic identifieri2162 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 684684Formate dehydrogenase subunit alphaPRO_0000063225Add
BLAST

Interactioni

Subunit structurei

Dimer of an alpha and a beta subunit.

Structurei

3D structure databases

ProteinModelPortaliP06131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 59574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006478. Formate_DH_asu.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01591. Fdh-alpha. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06131-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDIKYVPTIC PYCGVGCGMN LVVKDEKVVG VEPWKRHPVN EGKLCPKGNF
60 70 80 90 100
CYEIIHREDR LTTPLIKENG EFREATWDEA YDLIASKLGA YDPNEIGFFC
110 120 130 140 150
CARSPNENIY VNQKFARIVV GTHNIDHCAR LCHGPTVAGL AASFGSGAMT
160 170 180 190 200
NSYASFEDAD LIFSIGANSL EAHPLVGRKL MRAKMNGAYF IVADPRYTPT
210 220 230 240 250
AKQADQYIPF KTGTDVALMN AMMNVIISEG LEDKEFIEKR TKNYEELKEV
260 270 280 290 300
VSKYTPEMAE EITQVPADVI RDIAIKYAKA DKAAIVYSLG ITEHSHGVDN
310 320 330 340 350
VMQTANLAML TGNIGRLGTG VNPLRGQNNV QGACDMGALP TDYPGYRKVA
360 370 380 390 400
DQEVMEDVTC TWGCSDLGCE PGLKIPEMID AAAKGDLKVL YITGEDPVIS
410 420 430 440 450
DPDTHHVEEA LNNLDFFVVQ DIFMTDTAEF ADVVLPAACW AEQEGTFTNG
460 470 480 490 500
ERRVQLIRKA VDAPGESKYD WEIFCDLAKK MGADPEMFTY ESAQDIFEEV
510 520 530 540 550
RTVTPQYAGM NRERLDRPEA LHWPCPSEDH PGTAMMHIEK FAHPDGLGIF
560 570 580 590 600
MPLEEQGPME TPDDEYPLIL TTTRLLFHYH AAMTRRAATL DREVPTGYVE
610 620 630 640 650
INTEDAAELG IANKEKVKVK SRRGEIEIAA RVTDDIVKGI VNIPMHFREC
660 670 680
SANILTNAAA IDPKSGMPEY KACAVAISKM EGSK
Length:684
Mass (Da):75,717
Last modified:January 1, 1988 - v1
Checksum:i38D37CF86BFF06E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02581 Genomic DNA. Translation: AAA72182.1.
PIRiA24698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02581 Genomic DNA. Translation: AAA72182.1 .
PIRi A24698.

3D structure databases

ProteinModelPortali P06131.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P06131.

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR006478. Formate_DH_asu.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01591. Fdh-alpha. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum."
    Shuber A.P., Orr E.C., Recny M.A., Schendel P.F., May H.D., Schauer N.L., Ferry J.G.
    J. Biol. Chem. 261:12942-12947(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-26.
    Strain: JF-1.
  2. "Identification of formate dehydrogenase-specific mRNA species and nucleotide sequence of the fdhC gene of Methanobacterium formicicum."
    White W.B., Ferry J.G.
    J. Bacteriol. 174:4997-5004(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
    Strain: JF-1.

Entry informationi

Entry nameiFDHA_METFO
AccessioniPrimary (citable) accession number: P06131
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3