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Protein

Formate dehydrogenase subunit beta

Gene

fdhB

Organism
Methanobacterium formicicum
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of formate.

Catalytic activityi

Formate + NAD+ = CO2 + NADH.

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 2 [4Fe-4S] clusters.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi296 – 2961Iron-sulfur (4Fe-4S)By similarity
Metal bindingi299 – 2991Iron-sulfur (4Fe-4S)By similarity
Metal bindingi302 – 3021Iron-sulfur (4Fe-4S)By similarity
Metal bindingi306 – 3061Iron-sulfur (4Fe-4S)By similarity
Metal bindingi348 – 3481Iron-sulfur (4Fe-4S)By similarity
Metal bindingi351 – 3511Iron-sulfur (4Fe-4S)By similarity
Metal bindingi354 – 3541Iron-sulfur (4Fe-4S)By similarity
Metal bindingi358 – 3581Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. formate dehydrogenase (coenzyme F420) activity Source: MENGO
  3. formate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

SABIO-RKP06130.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase subunit beta (EC:1.2.1.2)
Gene namesi
Name:fdhB
OrganismiMethanobacterium formicicum
Taxonomic identifieri2162 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Formate dehydrogenase subunit betaPRO_0000159222Add
BLAST

Interactioni

Subunit structurei

Dimer of an alpha and a beta subunit.

Structurei

3D structure databases

ProteinModelPortaliP06130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini287 – 316304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini339 – 367294Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the FrhB family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR007516. Co_F420_Hydgase/DH_bsu_N.
IPR007525. FrhB_FdhB_C.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamiPF13183. Fer4_8. 1 hit.
PF04432. FrhB_FdhB_C. 1 hit.
PF04422. FrhB_FdhB_N. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINTNDMFYA KSSDAEIAEA GEYGGAVTTL LKFLLKEGIV DAVLAVDSSA
60 70 80 90 100
DLYDVVPILI EDPEDVVKAA GSLHFGTLNL AKVVTRYLDG AQDMKIAVTV
110 120 130 140 150
KPCDAMTMVE LMKREKVNAD NVIMVGLNCG GTMPPVKGRQ MMEEFYEVDP
160 170 180 190 200
DSVVKEEIAK GKLIVETEDG TEKEIPIDEL EDEGFGRRTN CRRCEVNIPR
210 220 230 240 250
MADLACGNWG VIGPLAGKAT FIEVCSPKGA EVLEKAKEAG VIDLEDPIPK
260 270 280 290 300
GIEIREKIDG AMVKLADKWQ GNDWEDKAGR EIFSVLTEYM DDFSRCLKCY
310 320 330 340 350
GCREACPICY CEDCCLEANN GPDWLSKGEI PPSPMFHLER MLHMVESCTN
360 370 380 390
CGQCEEVCPG EIPLAKIWHE VNAKMKDTFG YVKGTGDEKP PIAYFPVGK
Length:399
Mass (Da):43,922
Last modified:January 1, 1988 - v1
Checksum:iD57F778C6C8F67BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02581 Genomic DNA. Translation: AAA72183.1.
PIRiB24698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02581 Genomic DNA. Translation: AAA72183.1.
PIRiB24698.

3D structure databases

ProteinModelPortaliP06130.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP06130.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR007516. Co_F420_Hydgase/DH_bsu_N.
IPR007525. FrhB_FdhB_C.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamiPF13183. Fer4_8. 1 hit.
PF04432. FrhB_FdhB_C. 1 hit.
PF04422. FrhB_FdhB_N. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum."
    Shuber A.P., Orr E.C., Recny M.A., Schendel P.F., May H.D., Schauer N.L., Ferry J.G.
    J. Biol. Chem. 261:12942-12947(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22.
    Strain: JF-1.

Entry informationi

Entry nameiFDHB_METFO
AccessioniPrimary (citable) accession number: P06130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.