P06130 (FDHB_METFO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate dehydrogenase subunit beta
EC=1.2.1.2

Gene names

Name:

fdhB

Organism

Methanobacterium formicicum

Taxonomic identifier

2162 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobacterium

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation of formate.
Catalytic activity	Formate + NAD+ = CO2 + NADH.
Cofactor	Binds 2 4Fe-4S clusters Probable.
Subunit structure	Dimer of an alpha and a beta subunit.
Sequence similarities	Belongs to the FrhB family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW formate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Formate dehydrogenase subunit beta		PRO_0000159222
Regions
	Domain	287 – 316	30	4Fe-4S ferredoxin-type 1
	Domain	339 – 367	29	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	296	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	299	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	302	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	306	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	348	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	351	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	354	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	358	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06130 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: D57F778C6C8F67BB	FASTA	399	43,922
	10 20 30 40 50 60 MINTNDMFYA KSSDAEIAEA GEYGGAVTTL LKFLLKEGIV DAVLAVDSSA DLYDVVPILI 70 80 90 100 110 120 EDPEDVVKAA GSLHFGTLNL AKVVTRYLDG AQDMKIAVTV KPCDAMTMVE LMKREKVNAD 130 140 150 160 170 180 NVIMVGLNCG GTMPPVKGRQ MMEEFYEVDP DSVVKEEIAK GKLIVETEDG TEKEIPIDEL 190 200 210 220 230 240 EDEGFGRRTN CRRCEVNIPR MADLACGNWG VIGPLAGKAT FIEVCSPKGA EVLEKAKEAG 250 260 270 280 290 300 VIDLEDPIPK GIEIREKIDG AMVKLADKWQ GNDWEDKAGR EIFSVLTEYM DDFSRCLKCY 310 320 330 340 350 360 GCREACPICY CEDCCLEANN GPDWLSKGEI PPSPMFHLER MLHMVESCTN CGQCEEVCPG 370 380 390 EIPLAKIWHE VNAKMKDTFG YVKGTGDEKP PIAYFPVGK

References

[1]

"Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum." Shuber A.P., Orr E.C., Recny M.A., Schendel P.F., May H.D., Schauer N.L., Ferry J.G. J. Biol. Chem. 261:12942-12947(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22. Strain: JF-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02581 Genomic DNA. Translation: AAA72183.1.
PIR	B24698.
3D structure databases
ProteinModelPortal	P06130.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P06130.
Family and domain databases
Gene3D	1.10.1060.10. 1 hit.
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR007516. Co_F420_Hydgase/DH_bsu_N. IPR007525. FrhB_FdhB_C. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. [Graphical view]
Pfam	PF13183. Fer4_8. 1 hit. PF04432. FrhB_FdhB_C. 1 hit. PF04422. FrhB_FdhB_N. 1 hit. [Graphical view]
SUPFAM	SSF46548. Helical_ferredxn. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDHB_METFO

Accession

Primary (citable) accession number: P06130

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program