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Protein

Vitamin B12 transporter BtuB

Gene

btuB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB. Is also a receptor for bacteriophages BF23 and C1, and for A and E colicins.3 Publications

Enzyme regulationi

Calcium increases vitamin B12 binding affinity by a factor of 50-100.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi199Calcium 11 Publication1
Metal bindingi211Calcium 11 Publication1
Metal bindingi213Calcium 11 Publication1
Metal bindingi213Calcium 21 Publication1
Metal bindingi215Calcium 11 Publication1
Metal bindingi215Calcium 21 Publication1
Metal bindingi249Calcium 2; via carbonyl oxygen1 Publication1
Metal bindingi250Calcium 11 Publication1
Metal bindingi250Calcium 21 Publication1
Metal bindingi261Calcium 21 Publication1

GO - Molecular functioni

GO - Biological processi

  • cobalamin transport Source: EcoliWiki
  • ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Porin, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10126-MONOMER.
ECOL316407:JW3938-MONOMER.
MetaCyc:EG10126-MONOMER.

Protein family/group databases

TCDBi1.B.14.3.1. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12 transporter BtuBUniRule annotation
Alternative name(s):
Cobalamin receptorUniRule annotation
Outer membrane cobalamin translocatorUniRule annotation
Gene namesi
Name:btuB
Synonyms:bfe, cer, dcrC
Ordered Locus Names:b3966, JW3938
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10126. btuB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 157Periplasmic1 PublicationAdd BLAST137
Transmembranei158 – 165Beta stranded8
Topological domaini166 – 168Extracellular1 Publication3
Transmembranei169 – 178Beta stranded10
Topological domaini179 – 183Periplasmic1 Publication5
Transmembranei184 – 195Beta strandedAdd BLAST12
Topological domaini196 – 216Extracellular1 PublicationAdd BLAST21
Transmembranei217 – 227Beta strandedAdd BLAST11
Topological domaini228 – 231Periplasmic1 Publication4
Transmembranei232 – 248Beta strandedAdd BLAST17
Topological domaini249 – 262Extracellular1 PublicationAdd BLAST14
Transmembranei263 – 277Beta strandedAdd BLAST15
Topological domaini278Periplasmic1 Publication1
Transmembranei279 – 296Beta strandedAdd BLAST18
Topological domaini297 – 308Extracellular1 PublicationAdd BLAST12
Transmembranei309 – 325Beta strandedAdd BLAST17
Topological domaini326 – 327Periplasmic1 Publication2
Transmembranei328 – 337Beta stranded10
Topological domaini338 – 352Extracellular1 PublicationAdd BLAST15
Transmembranei353 – 369Beta strandedAdd BLAST17
Topological domaini370Periplasmic1 Publication1
Transmembranei371 – 381Beta strandedAdd BLAST11
Topological domaini382 – 384Extracellular1 Publication3
Transmembranei385 – 400Beta strandedAdd BLAST16
Topological domaini401 – 402Periplasmic1 Publication2
Transmembranei403 – 417Beta strandedAdd BLAST15
Topological domaini418 – 433Extracellular1 PublicationAdd BLAST16
Transmembranei434 – 443Beta stranded10
Topological domaini444 – 448Periplasmic1 Publication5
Transmembranei449 – 458Beta stranded10
Topological domaini459 – 472Extracellular1 PublicationAdd BLAST14
Transmembranei473 – 490Beta strandedAdd BLAST18
Topological domaini491 – 493Periplasmic1 Publication3
Transmembranei494 – 509Beta strandedAdd BLAST16
Topological domaini510 – 516Extracellular1 Publication7
Transmembranei517 – 529Beta strandedAdd BLAST13
Topological domaini530 – 534Periplasmic1 Publication5
Transmembranei535 – 550Beta strandedAdd BLAST16
Topological domaini551 – 557Extracellular1 Publication7
Transmembranei558 – 572Beta strandedAdd BLAST15
Topological domaini573 – 584Periplasmic1 PublicationAdd BLAST12
Transmembranei585 – 596Beta strandedAdd BLAST12
Topological domaini597 – 601Extracellular1 Publication5
Transmembranei602 – 614Beta strandedAdd BLAST13

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28L → P: Inactivates uptake. 1 Publication1
Mutagenesisi30V → G or P: Inactivates uptake. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 201 PublicationAdd BLAST20
ChainiPRO_000000347921 – 614Vitamin B12 transporter BtuBAdd BLAST594

Proteomic databases

PaxDbiP06129.
PRIDEiP06129.

Expressioni

Inductioni

Constitutively expressed. Primary control of btuB expression by cobalamin occurs at the level of translation initiation.1 Publication

Interactioni

Subunit structurei

Interacts with TonB.2 Publications

Protein-protein interaction databases

BioGridi4259524. 246 interactors.
DIPiDIP-9232N.
IntActiP06129. 1 interactor.
STRINGi511145.b3966.

Structurei

Secondary structure

1614
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 30Combined sources3
Turni31 – 33Combined sources3
Helixi39 – 41Combined sources3
Beta strandi46 – 50Combined sources5
Helixi51 – 57Combined sources7
Beta strandi60 – 62Combined sources3
Helixi63 – 66Combined sources4
Beta strandi72 – 75Combined sources4
Beta strandi85 – 88Combined sources4
Helixi93 – 95Combined sources3
Beta strandi96 – 100Combined sources5
Helixi106 – 108Combined sources3
Turni109 – 111Combined sources3
Helixi112 – 118Combined sources7
Helixi121 – 123Combined sources3
Beta strandi125 – 132Combined sources8
Helixi135 – 138Combined sources4
Beta strandi143 – 150Combined sources8
Beta strandi157 – 165Combined sources9
Turni166 – 168Combined sources3
Beta strandi169 – 181Combined sources13
Beta strandi184 – 195Combined sources12
Beta strandi205 – 207Combined sources3
Beta strandi217 – 248Combined sources32
Beta strandi255 – 257Combined sources3
Beta strandi262 – 277Combined sources16
Beta strandi279 – 297Combined sources19
Turni299 – 301Combined sources3
Beta strandi303 – 305Combined sources3
Beta strandi309 – 325Combined sources17
Beta strandi327 – 343Combined sources17
Helixi346 – 348Combined sources3
Helixi349 – 351Combined sources3
Beta strandi352 – 368Combined sources17
Beta strandi371 – 382Combined sources12
Turni383 – 385Combined sources3
Beta strandi386 – 400Combined sources15
Beta strandi403 – 414Combined sources12
Helixi418 – 422Combined sources5
Beta strandi423 – 426Combined sources4
Beta strandi433 – 446Combined sources14
Beta strandi449 – 467Combined sources19
Turni468 – 471Combined sources4
Beta strandi472 – 492Combined sources21
Beta strandi495 – 508Combined sources14
Turni509 – 511Combined sources3
Beta strandi520 – 531Combined sources12
Beta strandi534 – 543Combined sources10
Beta strandi546 – 550Combined sources5
Beta strandi552 – 555Combined sources4
Beta strandi557 – 561Combined sources5
Beta strandi564 – 587Combined sources24
Beta strandi605 – 613Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NQEX-ray2.00A21-614[»]
1NQFX-ray2.70A21-614[»]
1NQGX-ray3.31A21-614[»]
1NQHX-ray3.10A21-614[»]
1UJWX-ray2.75A21-614[»]
2GSKX-ray2.10A25-614[»]
2GUFX-ray1.95A21-614[»]
2YSUX-ray3.50A21-614[»]
3M8BX-ray2.44A21-614[»]
3M8DX-ray2.44A21-614[»]
3RGMX-ray2.60A21-614[»]
3RGNX-ray2.30A21-614[»]
ProteinModelPortaliP06129.
SMRiP06129.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06129.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 112Cobalamin-binding1 Publication5
Regioni249 – 251Cobalamin-binding1 Publication3
Regioni515 – 518Cobalamin-binding1 Publication4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi26 – 33TonB box8
Motifi597 – 614TonB C-terminal boxAdd BLAST18

Sequence similaritiesi

Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily. [View classification]UniRule annotationCurated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CHI. Bacteria.
COG4206. LUCA.
HOGENOMiHOG000269547.
InParanoidiP06129.
KOiK16092.
OMAiNRDIYST.
PhylomeDBiP06129.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
HAMAPiMF_01531. BtuB. 1 hit.
InterProiIPR010101. B12_transptr_BtuB.
IPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01779. TonB-B12. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKASLLTA CSVTAFSAWA QDTSPDTLVV TANRFEQPRS TVLAPTTVVT
60 70 80 90 100
RQDIDRWQST SVNDVLRRLP GVDITQNGGS GQLSSIFIRG TNASHVLVLI
110 120 130 140 150
DGVRLNLAGV SGSADLSQFP IALVQRVEYI RGPRSAVYGS DAIGGVVNII
160 170 180 190 200
TTRDEPGTEI SAGWGSNSYQ NYDVSTQQQL GDKTRVTLLG DYAHTHGYDV
210 220 230 240 250
VAYGNTGTQA QTDNDGFLSK TLYGALEHNF TDAWSGFVRG YGYDNRTNYD
260 270 280 290 300
AYYSPGSPLL DTRKLYSQSW DAGLRYNGEL IKSQLITSYS HSKDYNYDPH
310 320 330 340 350
YGRYDSSATL DEMKQYTVQW ANNVIVGHGS IGAGVDWQKQ TTTPGTGYVE
360 370 380 390 400
DGYDQRNTGI YLTGLQQVGD FTFEGAARSD DNSQFGRHGT WQTSAGWEFI
410 420 430 440 450
EGYRFIASYG TSYKAPNLGQ LYGFYGNPNL DPEKSKQWEG AFEGLTAGVN
460 470 480 490 500
WRISGYRNDV SDLIDYDDHT LKYYNEGKAR IKGVEATANF DTGPLTHTVS
510 520 530 540 550
YDYVDARNAI TDTPLLRRAK QQVKYQLDWQ LYDFDWGITY QYLGTRYDKD
560 570 580 590 600
YSSYPYQTVK MGGVSLWDLA VAYPVTSHLT VRGKIANLFD KDYETVYGYQ
610
TAGREYTLSG SYTF
Length:614
Mass (Da):68,407
Last modified:October 1, 1993 - v2
Checksum:iAB43CC46A991FF95
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162A → G in AAA23524 (PubMed:3882670).Curated1
Sequence conflicti377A → R in AAA23524 (PubMed:3882670).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10112 Genomic DNA. Translation: AAA23524.1.
U00006 Genomic DNA. Translation: AAC43072.1.
U00096 Genomic DNA. Translation: AAC76948.1.
AP009048 Genomic DNA. Translation: BAE77345.1.
M57568 Genomic DNA. No translation available.
L14556 Genomic DNA. Translation: AAA23676.1.
PIRiA65204. QRECBT.
RefSeqiNP_418401.1. NC_000913.3.
WP_000591359.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76948; AAC76948; b3966.
BAE77345; BAE77345; BAE77345.
GeneIDi948468.
KEGGiecj:JW3938.
eco:b3966.
PATRICi32123453. VBIEscCol129921_4087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10112 Genomic DNA. Translation: AAA23524.1.
U00006 Genomic DNA. Translation: AAC43072.1.
U00096 Genomic DNA. Translation: AAC76948.1.
AP009048 Genomic DNA. Translation: BAE77345.1.
M57568 Genomic DNA. No translation available.
L14556 Genomic DNA. Translation: AAA23676.1.
PIRiA65204. QRECBT.
RefSeqiNP_418401.1. NC_000913.3.
WP_000591359.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NQEX-ray2.00A21-614[»]
1NQFX-ray2.70A21-614[»]
1NQGX-ray3.31A21-614[»]
1NQHX-ray3.10A21-614[»]
1UJWX-ray2.75A21-614[»]
2GSKX-ray2.10A25-614[»]
2GUFX-ray1.95A21-614[»]
2YSUX-ray3.50A21-614[»]
3M8BX-ray2.44A21-614[»]
3M8DX-ray2.44A21-614[»]
3RGMX-ray2.60A21-614[»]
3RGNX-ray2.30A21-614[»]
ProteinModelPortaliP06129.
SMRiP06129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259524. 246 interactors.
DIPiDIP-9232N.
IntActiP06129. 1 interactor.
STRINGi511145.b3966.

Protein family/group databases

TCDBi1.B.14.3.1. the outer membrane receptor (omr) family.

Proteomic databases

PaxDbiP06129.
PRIDEiP06129.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76948; AAC76948; b3966.
BAE77345; BAE77345; BAE77345.
GeneIDi948468.
KEGGiecj:JW3938.
eco:b3966.
PATRICi32123453. VBIEscCol129921_4087.

Organism-specific databases

EchoBASEiEB0124.
EcoGeneiEG10126. btuB.

Phylogenomic databases

eggNOGiENOG4105CHI. Bacteria.
COG4206. LUCA.
HOGENOMiHOG000269547.
InParanoidiP06129.
KOiK16092.
OMAiNRDIYST.
PhylomeDBiP06129.

Enzyme and pathway databases

BioCyciEcoCyc:EG10126-MONOMER.
ECOL316407:JW3938-MONOMER.
MetaCyc:EG10126-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06129.
PROiP06129.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
HAMAPiMF_01531. BtuB. 1 hit.
InterProiIPR010101. B12_transptr_BtuB.
IPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01779. TonB-B12. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBTUB_ECOLI
AccessioniPrimary (citable) accession number: P06129
Secondary accession number(s): Q2M8R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.