Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vitamin B12 transporter BtuB

Gene

btuB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB. Is also a receptor for bacteriophages BF23 and C1, and for A and E colicins.3 Publications

Enzyme regulationi

Calcium increases vitamin B12 binding affinity by a factor of 50-100.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi199 – 1991Calcium 11 Publication
Metal bindingi211 – 2111Calcium 11 Publication
Metal bindingi213 – 2131Calcium 11 Publication
Metal bindingi213 – 2131Calcium 21 Publication
Metal bindingi215 – 2151Calcium 11 Publication
Metal bindingi215 – 2151Calcium 21 Publication
Metal bindingi249 – 2491Calcium 2; via carbonyl oxygen1 Publication
Metal bindingi250 – 2501Calcium 11 Publication
Metal bindingi250 – 2501Calcium 21 Publication
Metal bindingi261 – 2611Calcium 21 Publication

GO - Molecular functioni

GO - Biological processi

  • cobalamin transport Source: EcoliWiki
  • ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Porin, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10126-MONOMER.
ECOL316407:JW3938-MONOMER.
MetaCyc:EG10126-MONOMER.

Protein family/group databases

TCDBi1.B.14.3.1. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12 transporter BtuBUniRule annotation
Alternative name(s):
Cobalamin receptorUniRule annotation
Outer membrane cobalamin translocatorUniRule annotation
Gene namesi
Name:btuB
Synonyms:bfe, cer, dcrC
Ordered Locus Names:b3966, JW3938
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10126. btuB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 157137Periplasmic1 PublicationAdd
BLAST
Transmembranei158 – 1658Beta stranded
Topological domaini166 – 1683Extracellular1 Publication
Transmembranei169 – 17810Beta stranded
Topological domaini179 – 1835Periplasmic1 Publication
Transmembranei184 – 19512Beta strandedAdd
BLAST
Topological domaini196 – 21621Extracellular1 PublicationAdd
BLAST
Transmembranei217 – 22711Beta strandedAdd
BLAST
Topological domaini228 – 2314Periplasmic1 Publication
Transmembranei232 – 24817Beta strandedAdd
BLAST
Topological domaini249 – 26214Extracellular1 PublicationAdd
BLAST
Transmembranei263 – 27715Beta strandedAdd
BLAST
Topological domaini278 – 2781Periplasmic1 Publication
Transmembranei279 – 29618Beta strandedAdd
BLAST
Topological domaini297 – 30812Extracellular1 PublicationAdd
BLAST
Transmembranei309 – 32517Beta strandedAdd
BLAST
Topological domaini326 – 3272Periplasmic1 Publication
Transmembranei328 – 33710Beta stranded
Topological domaini338 – 35215Extracellular1 PublicationAdd
BLAST
Transmembranei353 – 36917Beta strandedAdd
BLAST
Topological domaini370 – 3701Periplasmic1 Publication
Transmembranei371 – 38111Beta strandedAdd
BLAST
Topological domaini382 – 3843Extracellular1 Publication
Transmembranei385 – 40016Beta strandedAdd
BLAST
Topological domaini401 – 4022Periplasmic1 Publication
Transmembranei403 – 41715Beta strandedAdd
BLAST
Topological domaini418 – 43316Extracellular1 PublicationAdd
BLAST
Transmembranei434 – 44310Beta stranded
Topological domaini444 – 4485Periplasmic1 Publication
Transmembranei449 – 45810Beta stranded
Topological domaini459 – 47214Extracellular1 PublicationAdd
BLAST
Transmembranei473 – 49018Beta strandedAdd
BLAST
Topological domaini491 – 4933Periplasmic1 Publication
Transmembranei494 – 50916Beta strandedAdd
BLAST
Topological domaini510 – 5167Extracellular1 Publication
Transmembranei517 – 52913Beta strandedAdd
BLAST
Topological domaini530 – 5345Periplasmic1 Publication
Transmembranei535 – 55016Beta strandedAdd
BLAST
Topological domaini551 – 5577Extracellular1 Publication
Transmembranei558 – 57215Beta strandedAdd
BLAST
Topological domaini573 – 58412Periplasmic1 PublicationAdd
BLAST
Transmembranei585 – 59612Beta strandedAdd
BLAST
Topological domaini597 – 6015Extracellular1 Publication
Transmembranei602 – 61413Beta strandedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi28 – 281L → P: Inactivates uptake. 1 Publication
Mutagenesisi30 – 301V → G or P: Inactivates uptake. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 PublicationAdd
BLAST
Chaini21 – 614594Vitamin B12 transporter BtuBPRO_0000003479Add
BLAST

Proteomic databases

PaxDbiP06129.
PRIDEiP06129.

Expressioni

Inductioni

Constitutively expressed. Primary control of btuB expression by cobalamin occurs at the level of translation initiation.1 Publication

Interactioni

Subunit structurei

Interacts with TonB.2 Publications

Protein-protein interaction databases

BioGridi4259524. 246 interactions.
DIPiDIP-9232N.
IntActiP06129. 1 interaction.
STRINGi511145.b3966.

Structurei

Secondary structure

1
614
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi28 – 303Combined sources
Turni31 – 333Combined sources
Helixi39 – 413Combined sources
Beta strandi46 – 505Combined sources
Helixi51 – 577Combined sources
Beta strandi60 – 623Combined sources
Helixi63 – 664Combined sources
Beta strandi72 – 754Combined sources
Beta strandi85 – 884Combined sources
Helixi93 – 953Combined sources
Beta strandi96 – 1005Combined sources
Helixi106 – 1083Combined sources
Turni109 – 1113Combined sources
Helixi112 – 1187Combined sources
Helixi121 – 1233Combined sources
Beta strandi125 – 1328Combined sources
Helixi135 – 1384Combined sources
Beta strandi143 – 1508Combined sources
Beta strandi157 – 1659Combined sources
Turni166 – 1683Combined sources
Beta strandi169 – 18113Combined sources
Beta strandi184 – 19512Combined sources
Beta strandi205 – 2073Combined sources
Beta strandi217 – 24832Combined sources
Beta strandi255 – 2573Combined sources
Beta strandi262 – 27716Combined sources
Beta strandi279 – 29719Combined sources
Turni299 – 3013Combined sources
Beta strandi303 – 3053Combined sources
Beta strandi309 – 32517Combined sources
Beta strandi327 – 34317Combined sources
Helixi346 – 3483Combined sources
Helixi349 – 3513Combined sources
Beta strandi352 – 36817Combined sources
Beta strandi371 – 38212Combined sources
Turni383 – 3853Combined sources
Beta strandi386 – 40015Combined sources
Beta strandi403 – 41412Combined sources
Helixi418 – 4225Combined sources
Beta strandi423 – 4264Combined sources
Beta strandi433 – 44614Combined sources
Beta strandi449 – 46719Combined sources
Turni468 – 4714Combined sources
Beta strandi472 – 49221Combined sources
Beta strandi495 – 50814Combined sources
Turni509 – 5113Combined sources
Beta strandi520 – 53112Combined sources
Beta strandi534 – 54310Combined sources
Beta strandi546 – 5505Combined sources
Beta strandi552 – 5554Combined sources
Beta strandi557 – 5615Combined sources
Beta strandi564 – 58724Combined sources
Beta strandi605 – 6139Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NQEX-ray2.00A21-614[»]
1NQFX-ray2.70A21-614[»]
1NQGX-ray3.31A21-614[»]
1NQHX-ray3.10A21-614[»]
1UJWX-ray2.75A21-614[»]
2GSKX-ray2.10A25-614[»]
2GUFX-ray1.95A21-614[»]
2YSUX-ray3.50A21-614[»]
3M8BX-ray2.44A21-614[»]
3M8DX-ray2.44A21-614[»]
3RGMX-ray2.60A21-614[»]
3RGNX-ray2.30A21-614[»]
ProteinModelPortaliP06129.
SMRiP06129. Positions 25-614.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06129.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni108 – 1125Cobalamin-binding1 Publication
Regioni249 – 2513Cobalamin-binding1 Publication
Regioni515 – 5184Cobalamin-binding1 Publication

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi26 – 338TonB box
Motifi597 – 61418TonB C-terminal boxAdd
BLAST

Sequence similaritiesi

Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily. [View classification]UniRule annotationCurated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CHI. Bacteria.
COG4206. LUCA.
HOGENOMiHOG000269547.
InParanoidiP06129.
KOiK16092.
OMAiNRDIYST.
PhylomeDBiP06129.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
HAMAPiMF_01531. BtuB. 1 hit.
InterProiIPR010101. B12_transptr_BtuB.
IPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01779. TonB-B12. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKASLLTA CSVTAFSAWA QDTSPDTLVV TANRFEQPRS TVLAPTTVVT
60 70 80 90 100
RQDIDRWQST SVNDVLRRLP GVDITQNGGS GQLSSIFIRG TNASHVLVLI
110 120 130 140 150
DGVRLNLAGV SGSADLSQFP IALVQRVEYI RGPRSAVYGS DAIGGVVNII
160 170 180 190 200
TTRDEPGTEI SAGWGSNSYQ NYDVSTQQQL GDKTRVTLLG DYAHTHGYDV
210 220 230 240 250
VAYGNTGTQA QTDNDGFLSK TLYGALEHNF TDAWSGFVRG YGYDNRTNYD
260 270 280 290 300
AYYSPGSPLL DTRKLYSQSW DAGLRYNGEL IKSQLITSYS HSKDYNYDPH
310 320 330 340 350
YGRYDSSATL DEMKQYTVQW ANNVIVGHGS IGAGVDWQKQ TTTPGTGYVE
360 370 380 390 400
DGYDQRNTGI YLTGLQQVGD FTFEGAARSD DNSQFGRHGT WQTSAGWEFI
410 420 430 440 450
EGYRFIASYG TSYKAPNLGQ LYGFYGNPNL DPEKSKQWEG AFEGLTAGVN
460 470 480 490 500
WRISGYRNDV SDLIDYDDHT LKYYNEGKAR IKGVEATANF DTGPLTHTVS
510 520 530 540 550
YDYVDARNAI TDTPLLRRAK QQVKYQLDWQ LYDFDWGITY QYLGTRYDKD
560 570 580 590 600
YSSYPYQTVK MGGVSLWDLA VAYPVTSHLT VRGKIANLFD KDYETVYGYQ
610
TAGREYTLSG SYTF
Length:614
Mass (Da):68,407
Last modified:October 1, 1993 - v2
Checksum:iAB43CC46A991FF95
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621A → G in AAA23524 (PubMed:3882670).Curated
Sequence conflicti377 – 3771A → R in AAA23524 (PubMed:3882670).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10112 Genomic DNA. Translation: AAA23524.1.
U00006 Genomic DNA. Translation: AAC43072.1.
U00096 Genomic DNA. Translation: AAC76948.1.
AP009048 Genomic DNA. Translation: BAE77345.1.
M57568 Genomic DNA. No translation available.
L14556 Genomic DNA. Translation: AAA23676.1.
PIRiA65204. QRECBT.
RefSeqiNP_418401.1. NC_000913.3.
WP_000591359.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76948; AAC76948; b3966.
BAE77345; BAE77345; BAE77345.
GeneIDi948468.
KEGGiecj:JW3938.
eco:b3966.
PATRICi32123453. VBIEscCol129921_4087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10112 Genomic DNA. Translation: AAA23524.1.
U00006 Genomic DNA. Translation: AAC43072.1.
U00096 Genomic DNA. Translation: AAC76948.1.
AP009048 Genomic DNA. Translation: BAE77345.1.
M57568 Genomic DNA. No translation available.
L14556 Genomic DNA. Translation: AAA23676.1.
PIRiA65204. QRECBT.
RefSeqiNP_418401.1. NC_000913.3.
WP_000591359.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NQEX-ray2.00A21-614[»]
1NQFX-ray2.70A21-614[»]
1NQGX-ray3.31A21-614[»]
1NQHX-ray3.10A21-614[»]
1UJWX-ray2.75A21-614[»]
2GSKX-ray2.10A25-614[»]
2GUFX-ray1.95A21-614[»]
2YSUX-ray3.50A21-614[»]
3M8BX-ray2.44A21-614[»]
3M8DX-ray2.44A21-614[»]
3RGMX-ray2.60A21-614[»]
3RGNX-ray2.30A21-614[»]
ProteinModelPortaliP06129.
SMRiP06129. Positions 25-614.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259524. 246 interactions.
DIPiDIP-9232N.
IntActiP06129. 1 interaction.
STRINGi511145.b3966.

Protein family/group databases

TCDBi1.B.14.3.1. the outer membrane receptor (omr) family.

Proteomic databases

PaxDbiP06129.
PRIDEiP06129.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76948; AAC76948; b3966.
BAE77345; BAE77345; BAE77345.
GeneIDi948468.
KEGGiecj:JW3938.
eco:b3966.
PATRICi32123453. VBIEscCol129921_4087.

Organism-specific databases

EchoBASEiEB0124.
EcoGeneiEG10126. btuB.

Phylogenomic databases

eggNOGiENOG4105CHI. Bacteria.
COG4206. LUCA.
HOGENOMiHOG000269547.
InParanoidiP06129.
KOiK16092.
OMAiNRDIYST.
PhylomeDBiP06129.

Enzyme and pathway databases

BioCyciEcoCyc:EG10126-MONOMER.
ECOL316407:JW3938-MONOMER.
MetaCyc:EG10126-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06129.
PROiP06129.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
HAMAPiMF_01531. BtuB. 1 hit.
InterProiIPR010101. B12_transptr_BtuB.
IPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01779. TonB-B12. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBTUB_ECOLI
AccessioniPrimary (citable) accession number: P06129
Secondary accession number(s): Q2M8R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.