Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homocysteine/cysteine synthase

Gene

MET17

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of O-acetyl-L-homoserine (OAH) into homocysteine in the methionine biosynthesis pathway. Also catalyzes the conversion of O-acetylserine (OAS) into cysteine, the last step in the cysteine biosynthesis pathway (PubMed:7765825, PubMed:4609980, PubMed:795806). However, it seems that in S.cerevisiae cysteine biosynthesis occurs exclusively through the cystathionine pathway and not via direct incorporation of sulfur into OAS (PubMed:1732168). It therefore has no metabolic role in cysteine biosynthesis and may only have a regulatory role controlling OAS levels (PubMed:12586406).2 Publications3 Publications

Catalytic activityi

O-acetyl-L-homoserine + methanethiol = L-methionine + acetate.4 Publications
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.3 Publications

Cofactori

pyridoxal 5'-phosphate4 Publications

Kineticsi

  1. KM=8.7 mM for O-acetyl-L-homoserine1 Publication
  2. KM=6.67 mM for O-acetyl-L-homoserine (at pH 7.8 in Tris-HCl buffer)1 Publication
  3. KM=5.12 mM for O-acetyl-L-serine (at pH 7.8 in potassium phospahte buffer)1 Publication

    pH dependencei

    Optimum pH is 7.8 (for O-acetylhomoserine sulfhydrylase activity in Tris-HCl buffer) and 8.4 (for both O-acetylhomoserine sulfhydrylase and O-acetylserine sulfhydrylase activities in barbital-HCl buffer).2 Publications

    Pathwayi: L-methionine biosynthesis via de novo pathway

    This protein is involved in the subpathway that synthesizes L-homocysteine from O-acetyl-L-homoserine.2 Publications This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

    GO - Molecular functioni

    • cysteine synthase activity Source: SGD
    • O-acetylhomoserine aminocarboxypropyltransferase activity Source: SGD
    • pyridoxal phosphate binding Source: GO_Central

    GO - Biological processi

    • cysteine biosynthetic process Source: SGD
    • cysteine biosynthetic process via cystathionine Source: GO_Central
    • methionine metabolic process Source: SGD
    • transsulfuration Source: GO_Central
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-387.
    YEAST:YLR303W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homocysteine/cysteine synthaseCurated (EC:2.5.1.471 Publication, EC:2.5.1.492 Publications)
    Alternative name(s):
    O-acetylserine/O-acetylhomoserine sulfhydrylase1 Publication
    Short name:
    OAS-OAH SHLase1 Publication
    Short name:
    OAS-OAH sulfhydrylase1 Publication
    Gene namesi
    Name:MET171 Publication
    Synonyms:MET151 Publication, MET251 Publication
    Ordered Locus Names:YLR303WImported
    ORF Names:L8003.1
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XII

    Organism-specific databases

    EuPathDBiFungiDB:YLR303W.
    SGDiS000004294. MET17.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemoved2 Publications
    Chaini2 – 444443Homocysteine/cysteine synthasePRO_0000114776Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei44 – 441PhosphoserineCombined sources
    Cross-linki160 – 160Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
    Modified residuei209 – 2091N6-(pyridoxal phosphate)lysineBy similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiP06106.

    PTM databases

    iPTMnetiP06106.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi31567. 29 interactions.
    DIPiDIP-1664N.
    IntActiP06106. 4 interactions.
    MINTiMINT-391893.

    Structurei

    3D structure databases

    ProteinModelPortaliP06106.
    SMRiP06106. Positions 5-435.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the trans-sulfuration enzymes family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00550000075611.
    HOGENOMiHOG000246417.
    InParanoidiP06106.
    KOiK17069.
    OMAiSHPYHET.
    OrthoDBiEOG092C2OYU.

    Family and domain databases

    CDDicd00614. CGS_like. 1 hit.
    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
    IPR006235. OAc-hSer/O-AcSer_sulfhydrylase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PANTHERiPTHR11808. PTHR11808. 2 hits.
    PTHR11808:SF12. PTHR11808:SF12. 2 hits.
    PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001434. CGS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01326. OAH_OAS_sulfhy. 1 hit.
    PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06106-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPSHFDTVQL HAGQENPGDN AHRSRAVPIY ATTSYVFENS KHGSQLFGLE
    60 70 80 90 100
    VPGYVYSRFQ NPTSNVLEER IAALEGGAAA LAVSSGQAAQ TLAIQGLAHT
    110 120 130 140 150
    GDNIVSTSYL YGGTYNQFKI SFKRFGIEAR FVEGDNPEEF EKVFDERTKA
    160 170 180 190 200
    VYLETIGNPK YNVPDFEKIV AIAHKHGIPV VVDNTFGAGG YFCQPIKYGA
    210 220 230 240 250
    DIVTHSATKW IGGHGTTIGG IIVDSGKFPW KDYPEKFPQF SQPAEGYHGT
    260 270 280 290 300
    IYNEAYGNLA YIVHVRTELL RDLGPLMNPF ASFLLLQGVE TLSLRAERHG
    310 320 330 340 350
    ENALKLAKWL EQSPYVSWVS YPGLASHSHH ENAKKYLSNG FGGVLSFGVK
    360 370 380 390 400
    DLPNADKETD PFKLSGAQVV DNLKLASNLA NVGDAKTLVI APYFTTHKQL
    410 420 430 440
    NDKEKLASGV TKDLIRVSVG IEFIDDIIAD FQQSFETVFA GQKP
    Length:444
    Mass (Da):48,672
    Last modified:January 23, 2007 - v3
    Checksum:iF84224625C921B35
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X04493 Genomic DNA. Translation: CAA28181.1.
    U17243 Genomic DNA. Translation: AAB67347.1.
    AY723848 Genomic DNA. Translation: AAU09765.1.
    BK006945 Genomic DNA. Translation: DAA09612.1.
    PIRiA25539.
    RefSeqiNP_013406.1. NM_001182191.1.

    Genome annotation databases

    EnsemblFungiiYLR303W; YLR303W; YLR303W.
    GeneIDi851010.
    KEGGisce:YLR303W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X04493 Genomic DNA. Translation: CAA28181.1.
    U17243 Genomic DNA. Translation: AAB67347.1.
    AY723848 Genomic DNA. Translation: AAU09765.1.
    BK006945 Genomic DNA. Translation: DAA09612.1.
    PIRiA25539.
    RefSeqiNP_013406.1. NM_001182191.1.

    3D structure databases

    ProteinModelPortaliP06106.
    SMRiP06106. Positions 5-435.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31567. 29 interactions.
    DIPiDIP-1664N.
    IntActiP06106. 4 interactions.
    MINTiMINT-391893.

    PTM databases

    iPTMnetiP06106.

    Proteomic databases

    PRIDEiP06106.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYLR303W; YLR303W; YLR303W.
    GeneIDi851010.
    KEGGisce:YLR303W.

    Organism-specific databases

    EuPathDBiFungiDB:YLR303W.
    SGDiS000004294. MET17.

    Phylogenomic databases

    GeneTreeiENSGT00550000075611.
    HOGENOMiHOG000246417.
    InParanoidiP06106.
    KOiK17069.
    OMAiSHPYHET.
    OrthoDBiEOG092C2OYU.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-387.
    YEAST:YLR303W-MONOMER.

    Miscellaneous databases

    PROiP06106.

    Family and domain databases

    CDDicd00614. CGS_like. 1 hit.
    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
    IPR006235. OAc-hSer/O-AcSer_sulfhydrylase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PANTHERiPTHR11808. PTHR11808. 2 hits.
    PTHR11808:SF12. PTHR11808:SF12. 2 hits.
    PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001434. CGS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01326. OAH_OAS_sulfhy. 1 hit.
    PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCYSD_YEAST
    AccessioniPrimary (citable) accession number: P06106
    Secondary accession number(s): D6VYU6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 23, 2007
    Last modified: September 7, 2016
    This is version 169 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.