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Protein

Protein MET17

Gene

MET17

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transforms O-acetylhomoserine into homocysteine and O-acetylserine into cysteine.

Catalytic activityi

O-acetyl-L-homoserine + methanethiol = L-methionine + acetate.
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Protein MET17 (MET17), Putative cysteine synthase (YGR012W)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • cysteine synthase activity Source: SGD
  • O-acetylhomoserine aminocarboxypropyltransferase activity Source: SGD
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • 'de novo' L-methionine biosynthetic process Source: GOC
  • cysteine biosynthetic process Source: SGD
  • methionine metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-387.
YEAST:YLR303W-MONOMER.
UniPathwayiUPA00136; UER00200.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MET17
Including the following 2 domains:
O-acetylhomoserine sulfhydrylase (EC:2.5.1.49)
Short name:
OAH sulfhydrylase
Alternative name(s):
Homocysteine synthase
O-acetylserine sulfhydrylase (EC:2.5.1.47)
Short name:
OAS sulfhydrylase
Gene namesi
Name:MET17
Synonyms:MET25
Ordered Locus Names:YLR303W
ORF Names:L8003.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR303W.
SGDiS000004294. MET17.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 444443Protein MET17PRO_0000114776Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441PhosphoserineCombined sources
Modified residuei209 – 2091N6-(pyridoxal phosphate)lysineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiP06106.
PRIDEiP06106.

PTM databases

iPTMnetiP06106.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi31567. 28 interactions.
DIPiDIP-1664N.
IntActiP06106. 4 interactions.
MINTiMINT-391893.

Structurei

3D structure databases

ProteinModelPortaliP06106.
SMRiP06106. Positions 5-435.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075611.
HOGENOMiHOG000246417.
InParanoidiP06106.
KOiK17069.
OMAiKMILRLV.
OrthoDBiEOG72ZCQ1.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR006235. OAc-hSer/O-AcSer_sulfhydrylase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PTHR11808:SF12. PTHR11808:SF12. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01326. OAH_OAS_sulfhy. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSHFDTVQL HAGQENPGDN AHRSRAVPIY ATTSYVFENS KHGSQLFGLE
60 70 80 90 100
VPGYVYSRFQ NPTSNVLEER IAALEGGAAA LAVSSGQAAQ TLAIQGLAHT
110 120 130 140 150
GDNIVSTSYL YGGTYNQFKI SFKRFGIEAR FVEGDNPEEF EKVFDERTKA
160 170 180 190 200
VYLETIGNPK YNVPDFEKIV AIAHKHGIPV VVDNTFGAGG YFCQPIKYGA
210 220 230 240 250
DIVTHSATKW IGGHGTTIGG IIVDSGKFPW KDYPEKFPQF SQPAEGYHGT
260 270 280 290 300
IYNEAYGNLA YIVHVRTELL RDLGPLMNPF ASFLLLQGVE TLSLRAERHG
310 320 330 340 350
ENALKLAKWL EQSPYVSWVS YPGLASHSHH ENAKKYLSNG FGGVLSFGVK
360 370 380 390 400
DLPNADKETD PFKLSGAQVV DNLKLASNLA NVGDAKTLVI APYFTTHKQL
410 420 430 440
NDKEKLASGV TKDLIRVSVG IEFIDDIIAD FQQSFETVFA GQKP
Length:444
Mass (Da):48,672
Last modified:January 23, 2007 - v3
Checksum:iF84224625C921B35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04493 Genomic DNA. Translation: CAA28181.1.
U17243 Genomic DNA. Translation: AAB67347.1.
AY723848 Genomic DNA. Translation: AAU09765.1.
BK006945 Genomic DNA. Translation: DAA09612.1.
PIRiA25539.
RefSeqiNP_013406.1. NM_001182191.1.

Genome annotation databases

EnsemblFungiiYLR303W; YLR303W; YLR303W.
GeneIDi851010.
KEGGisce:YLR303W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04493 Genomic DNA. Translation: CAA28181.1.
U17243 Genomic DNA. Translation: AAB67347.1.
AY723848 Genomic DNA. Translation: AAU09765.1.
BK006945 Genomic DNA. Translation: DAA09612.1.
PIRiA25539.
RefSeqiNP_013406.1. NM_001182191.1.

3D structure databases

ProteinModelPortaliP06106.
SMRiP06106. Positions 5-435.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31567. 28 interactions.
DIPiDIP-1664N.
IntActiP06106. 4 interactions.
MINTiMINT-391893.

PTM databases

iPTMnetiP06106.

Proteomic databases

PeptideAtlasiP06106.
PRIDEiP06106.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR303W; YLR303W; YLR303W.
GeneIDi851010.
KEGGisce:YLR303W.

Organism-specific databases

EuPathDBiFungiDB:YLR303W.
SGDiS000004294. MET17.

Phylogenomic databases

GeneTreeiENSGT00550000075611.
HOGENOMiHOG000246417.
InParanoidiP06106.
KOiK17069.
OMAiKMILRLV.
OrthoDBiEOG72ZCQ1.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.
BioCyciMetaCyc:MONOMER-387.
YEAST:YLR303W-MONOMER.

Miscellaneous databases

PROiP06106.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR006235. OAc-hSer/O-AcSer_sulfhydrylase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PTHR11808:SF12. PTHR11808:SF12. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01326. OAH_OAS_sulfhy. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Saccharomyces cerevisiae MET25 gene."
    Kerjan P., Cherest H., Surdin-Kerjan Y.
    Nucleic Acids Res. 14:7861-7871(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Cystathionine gamma-lyase of Saccharomyces cerevisiae: structural gene and cystathionine gamma-synthase activity."
    Ono B., Ishii N., Naito K., Miyoshi S., Shinoda S., Yamamoto S., Ohmori S.
    Yeast 9:389-397(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-10, CHARACTERIZATION.
  6. "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
    Norbeck J., Blomberg A.
    FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 131-140 AND 351-362.
    Strain: ATCC 38531 / Y41.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMET17_YEAST
AccessioniPrimary (citable) accession number: P06106
Secondary accession number(s): D6VYU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 167 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.