P06106 (MET17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein MET17

Including the following 2 domains:

1. O-acetylhomoserine sulfhydrylase
   Short name=OAH sulfhydrylase
   EC=2.5.1.49
   Alternative name(s):
   Homocysteine synthase
2. O-acetylserine sulfhydrylase
   Short name=OAS sulfhydrylase
   EC=2.5.1.47

Gene names

Name:	MET17
Synonyms:	MET25
Ordered Locus Names:	YLR303W
ORF Names:	L8003.1

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transforms O-acetylhomoserine into homocysteine and O-acetylserine into cysteine.
Catalytic activity	O-acetyl-L-homoserine + methanethiol = L-methionine + acetate. O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	O-acetylhomoserine aminocarboxypropyltransferase activity Inferred from direct assay. Source: SGD cysteine synthase activity Inferred from direct assay. Source: SGD pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 444	443	Protein MET17		PRO_0000114776
Amino acid modifications
	Modified residue	34	1	Phosphoserine Ref.9
	Modified residue	35	1	Phosphotyrosine Ref.9
	Modified residue	44	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	63	1	Phosphothreonine Ref.9
	Modified residue	209	1	N6-(pyridoxal phosphate)lysine By similarity
	Modified residue	377	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P06106 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F84224625C921B35	FASTA	444	48,672
	10 20 30 40 50 60 MPSHFDTVQL HAGQENPGDN AHRSRAVPIY ATTSYVFENS KHGSQLFGLE VPGYVYSRFQ 70 80 90 100 110 120 NPTSNVLEER IAALEGGAAA LAVSSGQAAQ TLAIQGLAHT GDNIVSTSYL YGGTYNQFKI 130 140 150 160 170 180 SFKRFGIEAR FVEGDNPEEF EKVFDERTKA VYLETIGNPK YNVPDFEKIV AIAHKHGIPV 190 200 210 220 230 240 VVDNTFGAGG YFCQPIKYGA DIVTHSATKW IGGHGTTIGG IIVDSGKFPW KDYPEKFPQF 250 260 270 280 290 300 SQPAEGYHGT IYNEAYGNLA YIVHVRTELL RDLGPLMNPF ASFLLLQGVE TLSLRAERHG 310 320 330 340 350 360 ENALKLAKWL EQSPYVSWVS YPGLASHSHH ENAKKYLSNG FGGVLSFGVK DLPNADKETD 370 380 390 400 410 420 PFKLSGAQVV DNLKLASNLA NVGDAKTLVI APYFTTHKQL NDKEKLASGV TKDLIRVSVG 430 440 IEFIDDIIAD FQQSFETVFA GQKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the Saccharomyces cerevisiae MET25 gene." Kerjan P., Cherest H., Surdin-Kerjan Y. Nucleic Acids Res. 14:7861-7871(1986) [PubMed: 3022238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Cystathionine gamma-lyase of Saccharomyces cerevisiae: structural gene and cystathionine gamma-synthase activity." Ono B., Ishii N., Naito K., Miyoshi S., Shinoda S., Yamamoto S., Ohmori S. Yeast 9:389-397(1993) [PubMed: 8511969] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-10, CHARACTERIZATION.
[6]	"Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae." Norbeck J., Blomberg A. FEMS Microbiol. Lett. 137:1-8(1996) [PubMed: 8935650] [Abstract] Cited for: PROTEIN SEQUENCE OF 131-140 AND 351-362. Strain: ATCC 38531 / Y41.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; TYR-35; SER-44; THR-63 AND SER-377, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04493 Genomic DNA. Translation: CAA28181.1. U17243 Genomic DNA. Translation: AAB67347.1. AY723848 Genomic DNA. Translation: AAU09765.1. BK006945 Genomic DNA. Translation: DAA09612.1.
PIR	A25539.
RefSeq	NP_013406.1. NM_001182191.1.
3D structure databases
ProteinModelPortal	P06106.
SMR	P06106. Positions 3-436.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1664N.
IntAct	P06106. 4 interactions.
MINT	MINT-391893.
STRING	P06106.
Proteomic databases
PeptideAtlas	P06106.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR303W; YLR303W; YLR303W.
GeneID	851010.
KEGG	sce:YLR303W.
NMPDR	fig|4932.3.peg.4424.
Organism-specific databases
SGD	S000004294. MET17.
Phylogenomic databases
eggNOG	fuNOG06155.
GeneTree	EFGT00050000003425.
HOGENOM	HBG754729.
OMA	PYFTTHQ.
OrthoDB	EOG4P8JSJ.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-387.
BRENDA	2.5.1.49. 984.
Gene expression databases
ArrayExpress	P06106.
Genevestigator	P06106.
GermOnline	YLR303W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR006235. OAc-hSer/O-AcSer_sulfhydrylase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 2 hits. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K01738. K01740.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit. PTHR11808:SF12. OAH_OAS_sulfhy. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01326. OAH_OAS_sulfhy. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967566.

Entry information

Entry name

MET17_YEAST

Accession

Primary (citable) accession number: P06106
Secondary accession number(s): D6VYU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 129 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program