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Protein

Homocysteine/cysteine synthase

Gene

MET17

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of O-acetyl-L-homoserine (OAH) into homocysteine in the methionine biosynthesis pathway. Also catalyzes the conversion of O-acetylserine (OAS) into cysteine, the last step in the cysteine biosynthesis pathway (PubMed:7765825, PubMed:4609980, PubMed:795806). However, it seems that in S.cerevisiae cysteine biosynthesis occurs exclusively through the cystathionine pathway and not via direct incorporation of sulfur into OAS (PubMed:1732168). It therefore has no metabolic role in cysteine biosynthesis and may only have a regulatory role controlling OAS levels (PubMed:12586406).2 Publications3 Publications

Catalytic activityi

O-acetyl-L-homoserine + methanethiol = L-methionine + acetate.4 Publications
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.3 Publications

Cofactori

pyridoxal 5'-phosphate4 Publications

Kineticsi

  1. KM=8.7 mM for O-acetyl-L-homoserine1 Publication
  2. KM=6.67 mM for O-acetyl-L-homoserine (at pH 7.8 in Tris-HCl buffer)1 Publication
  3. KM=5.12 mM for O-acetyl-L-serine (at pH 7.8 in potassium phosphate buffer)1 Publication

    pH dependencei

    Optimum pH is 7.8 (for O-acetylhomoserine sulfhydrylase activity in Tris-HCl buffer) and 8.4 (for both O-acetylhomoserine sulfhydrylase and O-acetylserine sulfhydrylase activities in barbital-HCl buffer).2 Publications

    Pathwayi: L-methionine biosynthesis via de novo pathway

    This protein is involved in the subpathway that synthesizes L-homocysteine from O-acetyl-L-homoserine.2 Publications This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

    GO - Molecular functioni

    • cysteine synthase activity Source: SGD
    • O-acetylhomoserine aminocarboxypropyltransferase activity Source: SGD
    • pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    • cysteine biosynthetic process Source: SGD
    • methionine metabolic process Source: SGD

    Keywordsi

    Molecular functionMultifunctional enzyme, Transferase
    Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:YLR303W-MONOMER
    YEAST:YLR303W-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homocysteine/cysteine synthaseCurated (EC:2.5.1.471 Publication, EC:2.5.1.492 Publications)
    Alternative name(s):
    O-acetylserine/O-acetylhomoserine sulfhydrylase1 Publication
    Short name:
    OAS-OAH SHLase1 Publication
    Short name:
    OAS-OAH sulfhydrylase1 Publication
    Gene namesi
    Name:MET171 Publication
    Synonyms:MET151 Publication, MET251 Publication
    Ordered Locus Names:YLR303WImported
    ORF Names:L8003.1
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XII

    Organism-specific databases

    EuPathDBiFungiDB:YLR303W
    SGDiS000004294 MET17

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved2 Publications
    ChainiPRO_00001147762 – 444Homocysteine/cysteine synthaseAdd BLAST443

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei44PhosphoserineCombined sources1
    Cross-linki160Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
    Modified residuei209N6-(pyridoxal phosphate)lysineBy similarity1

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP06106
    PRIDEiP06106

    PTM databases

    CarbonylDBiP06106
    iPTMnetiP06106

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PTA1Q013292EBI-11487,EBI-14145

    Protein-protein interaction databases

    BioGridi31567, 81 interactors
    DIPiDIP-1664N
    IntActiP06106, 7 interactors
    MINTiP06106
    STRINGi4932.YLR303W

    Structurei

    3D structure databases

    ProteinModelPortaliP06106
    SMRiP06106
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the trans-sulfuration enzymes family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00550000075611
    HOGENOMiHOG000246417
    InParanoidiP06106
    KOiK17069
    OMAiNPRFNIP
    OrthoDBiEOG092C2OYU

    Family and domain databases

    CDDicd00614 CGS_like, 1 hit
    Gene3Di3.40.640.10, 1 hit
    3.90.1150.10, 1 hit
    InterProiView protein in InterPro
    IPR000277 Cys/Met-Metab_PyrdxlP-dep_enz
    IPR006235 OAc-hSer/O-AcSer_sulfhydrylase
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    PfamiView protein in Pfam
    PF01053 Cys_Met_Meta_PP, 1 hit
    PIRSFiPIRSF001434 CGS, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit
    TIGRFAMsiTIGR01326 OAH_OAS_sulfhy, 1 hit
    PROSITEiView protein in PROSITE
    PS00868 CYS_MET_METAB_PP, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06106-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPSHFDTVQL HAGQENPGDN AHRSRAVPIY ATTSYVFENS KHGSQLFGLE
    60 70 80 90 100
    VPGYVYSRFQ NPTSNVLEER IAALEGGAAA LAVSSGQAAQ TLAIQGLAHT
    110 120 130 140 150
    GDNIVSTSYL YGGTYNQFKI SFKRFGIEAR FVEGDNPEEF EKVFDERTKA
    160 170 180 190 200
    VYLETIGNPK YNVPDFEKIV AIAHKHGIPV VVDNTFGAGG YFCQPIKYGA
    210 220 230 240 250
    DIVTHSATKW IGGHGTTIGG IIVDSGKFPW KDYPEKFPQF SQPAEGYHGT
    260 270 280 290 300
    IYNEAYGNLA YIVHVRTELL RDLGPLMNPF ASFLLLQGVE TLSLRAERHG
    310 320 330 340 350
    ENALKLAKWL EQSPYVSWVS YPGLASHSHH ENAKKYLSNG FGGVLSFGVK
    360 370 380 390 400
    DLPNADKETD PFKLSGAQVV DNLKLASNLA NVGDAKTLVI APYFTTHKQL
    410 420 430 440
    NDKEKLASGV TKDLIRVSVG IEFIDDIIAD FQQSFETVFA GQKP
    Length:444
    Mass (Da):48,672
    Last modified:January 23, 2007 - v3
    Checksum:iF84224625C921B35
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X04493 Genomic DNA Translation: CAA28181.1
    U17243 Genomic DNA Translation: AAB67347.1
    AY723848 Genomic DNA Translation: AAU09765.1
    BK006945 Genomic DNA Translation: DAA09612.1
    PIRiA25539
    RefSeqiNP_013406.1, NM_001182191.1

    Genome annotation databases

    EnsemblFungiiYLR303W; YLR303W; YLR303W
    GeneIDi851010
    KEGGisce:YLR303W

    Similar proteinsi

    Entry informationi

    Entry nameiCYSD_YEAST
    AccessioniPrimary (citable) accession number: P06106
    Secondary accession number(s): D6VYU6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 23, 2007
    Last modified: March 28, 2018
    This is version 182 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health