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Protein

Ubiquitin-conjugating enzyme E2 2

Gene

RAD6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. In association with the E3 enzyme BRE1 and LGE1, it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In association with the E3 enzyme RAD18, it catalyzes the monoubiquitination of POL30 'Lys-164', involved in postreplication repair of UV-damaged DNA. The RAD6/UBC2-RAD18 complex is also involved in prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine. In association with the E3 enzyme UBR1, is involved in N-end rule-dependent protein degradation. Also involved in sporulation.PROSITE-ProRule annotation17 Publications

Miscellaneous

Present with 2770 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

  • chromatin silencing at telomere Source: SGD
  • cytoplasm protein quality control by the ubiquitin-proteasome system Source: SGD
  • DNA-templated transcription, termination Source: SGD
  • double-strand break repair via homologous recombination Source: SGD
  • error-free postreplication DNA repair Source: SGD
  • error-free translesion synthesis Source: SGD
  • error-prone translesion synthesis Source: SGD
  • histone monoubiquitination Source: SGD
  • meiotic DNA double-strand break formation Source: SGD
  • mitotic G1 DNA damage checkpoint Source: SGD
  • protein K63-linked ubiquitination Source: SGD
  • protein monoubiquitination Source: SGD
  • protein polyubiquitination Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • regulation of dipeptide transport Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • telomere maintenance via recombination Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD
  • ubiquitin-dependent ERAD pathway Source: SGD
  • ubiquitin-dependent protein catabolic process via the N-end rule pathway Source: SGD

Keywordsi

Molecular functionChromatin regulator, Transferase
Biological processDNA damage, DNA repair, Sporulation, Transcription, Transcription regulation, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30566-MONOMER.
ReactomeiR-SCE-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-SCE-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-SCE-8866654. E3 ubiquitin ligases ubiquitinate target proteins.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 2 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 2
Radiation sensitivity protein 6
Ubiquitin carrier protein UBC2
Ubiquitin-protein ligase UBC2
Gene namesi
Name:RAD6
Synonyms:UBC2
Ordered Locus Names:YGL058W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL058W.
SGDiS000003026. RAD6.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 9Missing : Prevents H3K4me3 formation. 2 Publications9
Mutagenesisi88C → A or V: Loss of activity. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825401 – 172Ubiquitin-conjugating enzyme E2 2Add BLAST172

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei120Phosphoserine; by SGV1Combined sources1 Publication1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP06104.

PTM databases

iPTMnetiP06104.

Expressioni

Inductioni

Up-regulated by UV radiations and during meiosis.1 Publication

Interactioni

Subunit structurei

Forms a heterodimer complexes with the E3 enzymes BRE1, RAD18 and UBR1. Interacts also with UBR2, RTF1, PAF1 and the RNA polymerase II hyperphosphorylated form. The interaction with RNA polymerase II is BRE1- and PAF1-dependent.6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33189. 398 interactors.
DIPiDIP-1555N.
IntActiP06104. 13 interactors.
MINTiMINT-394584.
STRINGi4932.YGL058W.

Structurei

Secondary structure

1172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Beta strandi24 – 29Combined sources6
Beta strandi32 – 41Combined sources10
Turni47 – 50Combined sources4
Beta strandi52 – 58Combined sources7
Turni61 – 65Combined sources5
Beta strandi69 – 74Combined sources6
Beta strandi85 – 87Combined sources3
Helixi90 – 92Combined sources3
Turni93 – 95Combined sources3
Helixi102 – 114Combined sources13
Helixi124 – 132Combined sources9
Helixi134 – 149Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AYZX-ray2.60A/B/C1-172[»]
4R62X-ray2.28A1-172[»]
ProteinModelPortaliP06104.
SMRiP06104.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06104.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi150 – 172Asp/Glu-rich (acidic tail)Add BLAST23

Domaini

The acidic-tail domain of UBC2 mediates interaction with UBR1 and UBR2, and thus is important for polyubiquitination of histones. This domain is also important for sporulation.

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00900000140930.
HOGENOMiHOG000233454.
InParanoidiP06104.
KOiK10573.
OMAiRENMKEY.
OrthoDBiEOG092C50OL.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P06104-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPARRRLM RDFKRMKEDA PPGVSASPLP DNVMVWNAMI IGPADTPYED
60 70 80 90 100
GTFRLLLEFD EEYPNKPPHV KFLSEMFHPN VYANGEICLD ILQNRWTPTY
110 120 130 140 150
DVASILTSIQ SLFNDPNPAS PANVEAATLF KDHKSQYVKR VKETVEKSWE
160 170
DDMDDMDDDD DDDDDDDDDE AD
Length:172
Mass (Da):19,706
Last modified:January 1, 1988 - v1
Checksum:i5F568DC28ABBD60F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02962 Genomic DNA. Translation: AAA34952.1.
Z72580 Genomic DNA. Translation: CAA96761.1.
BK006941 Genomic DNA. Translation: DAA08044.1.
PIRiA21906.
RefSeqiNP_011457.1. NM_001180923.1.

Genome annotation databases

EnsemblFungiiYGL058W; YGL058W; YGL058W.
GeneIDi852822.
KEGGisce:YGL058W.

Similar proteinsi

Entry informationi

Entry nameiUBC2_YEAST
AccessioniPrimary (citable) accession number: P06104
Secondary accession number(s): D6VU83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 25, 2017
This is version 186 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names