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Protein

Eukaryotic translation initiation factor 3 subunit B

Gene

PRT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.UniRule annotation1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • translation initiation factor activity Source: SGD

GO - Biological processi

  • cytoplasmic translational initiation Source: SGD
  • formation of translation preinitiation complex Source: UniProtKB-HAMAP
  • regulation of translational initiation Source: UniProtKB-HAMAP
  • translational initiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33831-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit BUniRule annotation
Short name:
eIF3bUniRule annotation
Alternative name(s):
Cell cycle regulation and translation initiation protein
Eukaryotic translation initiation factor 3 90 kDa subunit
Short name:
eIF3 p90UniRule annotation
Translation initiation factor eIF3 p90 subunit
Gene namesi
Name:PRT1UniRule annotation
Synonyms:CDC63
Ordered Locus Names:YOR361C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR361C.
SGDiS000005888. PRT1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
  • eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
  • eukaryotic 48S preinitiation complex Source: SGD
  • eukaryotic translation initiation factor 3 complex Source: SGD
  • multi-eIF complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi124 – 130KGFLFVE → AAAAAAA: Impairs interaction with HCR1 and TIF32, impairs interaction of the eIF-3 complex with eIF-1, eIF-2 and the 40S ribosome, and impairs initiation of translation. 1 Publication7

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001235351 – 763Eukaryotic translation initiation factor 3 subunit BAdd BLAST763

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphoserineCombined sources1
Modified residuei67PhosphotyrosineCombined sources1
Modified residuei669PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06103.
PRIDEiP06103.

PTM databases

iPTMnetiP06103.

Interactioni

Subunit structurei

The eukaryotic translation initiation factor 3 (eIF-3) core complex is composed of TIF32, PRT1, NIP1, TIF34 and TIF35. A subcomplex of TIF32, NIP1 and PRT1 mediates the interaction with eIF-1, TIF5/eIF-5 and HCR1. The factors eIF-1, eIF-2, eIF-3, TIF5/eIF-5 and methionyl-tRNAi form a multifactor complex (MFC) that may bind to the 40S ribosome.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NIP1P324979EBI-8973,EBI-8965
RPG1P3824911EBI-8973,EBI-8981
TIF34P4021714EBI-8973,EBI-8951
TIF35Q0406710EBI-8973,EBI-8958

Protein-protein interaction databases

BioGridi34746. 118 interactors.
DIPiDIP-2519N.
IntActiP06103. 44 interactors.
MINTiMINT-578476.

Structurei

Secondary structure

1763
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi78 – 83Combined sources6
Helixi89 – 91Combined sources3
Helixi92 – 104Combined sources13
Beta strandi109 – 113Combined sources5
Turni118 – 121Combined sources4
Beta strandi125 – 133Combined sources9
Helixi134 – 144Combined sources11
Beta strandi148 – 152Combined sources5
Beta strandi156 – 160Combined sources5
Helixi161 – 168Combined sources8
Helixi186 – 189Combined sources4
Helixi191 – 194Combined sources4
Beta strandi201 – 205Combined sources5
Beta strandi207 – 214Combined sources8
Beta strandi224 – 234Combined sources11
Beta strandi236 – 238Combined sources3
Beta strandi245 – 248Combined sources4
Beta strandi250 – 257Combined sources8
Turni258 – 261Combined sources4
Beta strandi262 – 268Combined sources7
Beta strandi272 – 277Combined sources6
Beta strandi283 – 290Combined sources8
Helixi305 – 307Combined sources3
Beta strandi312 – 316Combined sources5
Turni317 – 319Combined sources3
Beta strandi322 – 326Combined sources5
Beta strandi338 – 341Combined sources4
Beta strandi345 – 352Combined sources8
Beta strandi355 – 360Combined sources6
Helixi361 – 363Combined sources3
Helixi371 – 373Combined sources3
Beta strandi380 – 383Combined sources4
Beta strandi395 – 397Combined sources3
Beta strandi400 – 407Combined sources8
Beta strandi411 – 413Combined sources3
Beta strandi416 – 422Combined sources7
Beta strandi427 – 432Combined sources6
Beta strandi435 – 443Combined sources9
Beta strandi447 – 457Combined sources11
Beta strandi464 – 472Combined sources9
Beta strandi480 – 484Combined sources5
Beta strandi486 – 494Combined sources9
Beta strandi501 – 506Combined sources6
Beta strandi519 – 527Combined sources9
Beta strandi538 – 547Combined sources10
Beta strandi551 – 554Combined sources4
Beta strandi558 – 565Combined sources8
Beta strandi571 – 573Combined sources3
Beta strandi575 – 580Combined sources6
Beta strandi593 – 596Combined sources4
Beta strandi600 – 603Combined sources4
Beta strandi611 – 615Combined sources5
Beta strandi622 – 626Combined sources5
Turni628 – 630Combined sources3
Beta strandi636 – 641Combined sources6
Beta strandi646 – 651Combined sources6
Beta strandi657 – 660Combined sources4
Helixi694 – 728Combined sources35

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JAPelectron microscopy4.90r704-734[»]
3JAQelectron microscopy6.00r704-737[»]
3NS5X-ray2.60A/B76-161[»]
3NS6X-ray1.25A/B76-170[»]
3ZWLX-ray2.20E/F693-739[»]
4U1EX-ray2.00B694-737[»]
4U1FX-ray2.20A/B172-665[»]
ProteinModelPortaliP06103.
SMRiP06103.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06103.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 162RRMUniRule annotationAdd BLAST86
Repeati228 – 266WD 1Add BLAST39
Repeati277 – 325WD 2Add BLAST49
Repeati373 – 416WD 3Add BLAST44
Repeati484 – 524WD 4Add BLAST41
Repeati544 – 589WD 5Add BLAST46
Repeati605 – 650WD 6Add BLAST46

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 136Sufficient for interaction with HCR1 and TIF321 PublicationAdd BLAST136
Regioni28 – 261Sufficient for interaction with PIC8Add BLAST234

Sequence similaritiesi

Belongs to the eIF-3 subunit B family.UniRule annotation
Contains 1 RRM (RNA recognition motif) domain.UniRule annotation
Contains 6 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00550000074913.
HOGENOMiHOG000265546.
InParanoidiP06103.
KOiK03253.
OMAiNNSACTA.
OrthoDBiEOG092C0KCM.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 2 hits.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06103-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNFLPRTLK NIYELYFNNI SVHSIVSRNT QLKRSKIIQM TTETFEDIKL
60 70 80 90 100
EDIPVDDIDF SDLEEQYKVT EEFNFDQYIV VNGAPVIPSA KVPVLKKALT
110 120 130 140 150
SLFSKAGKVV NMEFPIDEAT GKTKGFLFVE CGSMNDAKKI IKSFHGKRLD
160 170 180 190 200
LKHRLFLYTM KDVERYNSDD FDTEFREPDM PTFVPSSSLK SWLMDDKVRD
210 220 230 240 250
QFVLQDDVKT SVFWNSMFNE EDSLVESREN WSTNYVRFSP KGTYLFSYHQ
260 270 280 290 300
QGVTAWGGPN FDRLRRFYHP DVRNSSVSPN EKYLVTFSTE PIIVEEDNEF
310 320 330 340 350
SPFTKKNEGH QLCIWDIASG LLMATFPVIK SPYLKWPLVR WSYNDKYCAR
360 370 380 390 400
MVGDSLIVHD ATKNFMPLEA KALKPSGIRD FSFAPEGVKL QPFRNGDEPS
410 420 430 440 450
VLLAYWTPET NNSACTATIA EVPRGRVLKT VNLVQVSNVT LHWQNQAEFL
460 470 480 490 500
CFNVERHTKS GKTQFSNLQI CRLTERDIPV EKVELKDSVF EFGWEPHGNR
510 520 530 540 550
FVTISVHEVA DMNYAIPANT IRFYAPETKE KTDVIKRWSL VKEIPKTFAN
560 570 580 590 600
TVSWSPAGRF VVVGALVGPN MRRSDLQFYD MDYPGEKNIN DNNDVSASLK
610 620 630 640 650
DVAHPTYSAA TNITWDPSGR YVTAWSSSLK HKVEHGYKIF NIAGNLVKED
660 670 680 690 700
IIAGFKNFAW RPRPASILSN AERKKVRKNL REWSAQFEEQ DAMEADTAMR
710 720 730 740 750
DLILHQRELL KQWTEYREKI GQEMEKSMNF KIFDVQPEDA SDDFTTIEEI
760
VEEVLEETKE KVE
Length:763
Mass (Da):88,130
Last modified:January 1, 1988 - v1
Checksum:iF5E4E783408EE948
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02674 Genomic DNA. Translation: AAA34917.1.
Z75269 Genomic DNA. Translation: CAA99690.1.
BK006948 Genomic DNA. Translation: DAA11122.1.
PIRiA29562.
RefSeqiNP_015006.3. NM_001183781.3.

Genome annotation databases

EnsemblFungiiYOR361C; YOR361C; YOR361C.
GeneIDi854543.
KEGGisce:YOR361C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02674 Genomic DNA. Translation: AAA34917.1.
Z75269 Genomic DNA. Translation: CAA99690.1.
BK006948 Genomic DNA. Translation: DAA11122.1.
PIRiA29562.
RefSeqiNP_015006.3. NM_001183781.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JAPelectron microscopy4.90r704-734[»]
3JAQelectron microscopy6.00r704-737[»]
3NS5X-ray2.60A/B76-161[»]
3NS6X-ray1.25A/B76-170[»]
3ZWLX-ray2.20E/F693-739[»]
4U1EX-ray2.00B694-737[»]
4U1FX-ray2.20A/B172-665[»]
ProteinModelPortaliP06103.
SMRiP06103.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34746. 118 interactors.
DIPiDIP-2519N.
IntActiP06103. 44 interactors.
MINTiMINT-578476.

PTM databases

iPTMnetiP06103.

Proteomic databases

MaxQBiP06103.
PRIDEiP06103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR361C; YOR361C; YOR361C.
GeneIDi854543.
KEGGisce:YOR361C.

Organism-specific databases

EuPathDBiFungiDB:YOR361C.
SGDiS000005888. PRT1.

Phylogenomic databases

GeneTreeiENSGT00550000074913.
HOGENOMiHOG000265546.
InParanoidiP06103.
KOiK03253.
OMAiNNSACTA.
OrthoDBiEOG092C0KCM.

Enzyme and pathway databases

BioCyciYEAST:G3O-33831-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

EvolutionaryTraceiP06103.
PROiP06103.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 2 hits.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3B_YEAST
AccessioniPrimary (citable) accession number: P06103
Secondary accession number(s): D6W356
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 172 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 47500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.