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Protein

General negative regulator of transcription subunit 3

Gene

NOT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

GO - Biological processi

  • deadenylation-dependent decapping of nuclear-transcribed mRNA Source: SGD
  • negative regulation of translation Source: GO_Central
  • nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • protein ubiquitination Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31310-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 3
Gene namesi
Name:NOT3
Ordered Locus Names:YIL038C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL038C.
SGDiS000001300. NOT3.

Subcellular locationi

GO - Cellular componenti

  • CCR4-NOT core complex Source: SGD
  • cytoplasm Source: SGD
  • cytoplasmic mRNA processing body Source: GO_Central
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983371 – 836General negative regulator of transcription subunit 3Add BLAST836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei303PhosphoserineCombined sources1
Modified residuei307PhosphoserineCombined sources1
Modified residuei322PhosphoserineCombined sources1
Modified residuei446PhosphoserineCombined sources1
Modified residuei450PhosphoserineCombined sources1
Cross-linki535Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei565PhosphoserineCombined sources1
Modified residuei569PhosphoserineCombined sources1
Modified residuei571PhosphothreonineCombined sources1
Modified residuei657PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP06102.
PRIDEiP06102.
TopDownProteomicsiP06102.

PTM databases

iPTMnetiP06102.

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAF40P538293EBI-12165,EBI-28306
CDC39P256553EBI-12165,EBI-12139

Protein-protein interaction databases

BioGridi34952. 116 interactors.
DIPiDIP-2256N.
IntActiP06102. 45 interactors.
MINTiMINT-426406.

Structurei

3D structure databases

ProteinModelPortaliP06102.
SMRiP06102.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili36 – 68Sequence analysisAdd BLAST33
Coiled coili119 – 195Sequence analysisAdd BLAST77
Coiled coili255 – 292Sequence analysisAdd BLAST38
Coiled coili803 – 831Sequence analysisAdd BLAST29

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000014743.
HOGENOMiHOG000113831.
InParanoidiP06102.
KOiK12580.
OMAiMAHRKLQ.
OrthoDBiEOG092C3ROC.

Family and domain databases

InterProiIPR012270. CCR4-NOT_su3/5.
IPR007282. NOT.
IPR007207. Not_N.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view]
PIRSFiPIRSF005290. NOT_su_3_5. 1 hit.

Sequencei

Sequence statusi: Complete.

P06102-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHRKLQQEV DRVFKKINEG LEIFNSYYER HESCTNNPSQ KDKLESDLKR
60 70 80 90 100
EVKKLQRLRE QIKSWQSSPD IKDKDSLLDY RRSVEIAMEK YKAVEKASKE
110 120 130 140 150
KAYSNISLKK SETLDPQERE RRDISEYLSQ MIDELERQYD SLQVEIDKLL
160 170 180 190 200
LLNKKKKTSS TTNDEKKEQY KRFQARYRWH QQQMELALRL LANEELDPQD
210 220 230 240 250
VKNVQDDINY FVESNQDPDF VEDETIYDGL NLQSNEAIAH EVAQYFASQN
260 270 280 290 300
AEDNNTSDAN ESLQDISKLS KKEQRKLERE AKKAAKLAAK NATGAAIPVA
310 320 330 340 350
GPSSTPSPVI PVADASKETE RSPSSSPIHN ATKPEEAVKT SIKSPRSSAD
360 370 380 390 400
NLLPSLQKSP SSATPETPTN VHTHIHQTPN GITGATTLKP ATLPAKPAGE
410 420 430 440 450
LKWAVAASQA VEKDRKVTSA SSTISNTSTK TPTTAAATTT SSNANSRIGS
460 470 480 490 500
ALNTPKLSTS SLSLQPDNTG ASSSAATAAA VLAAGAAAVH QNNQAFYRNM
510 520 530 540 550
SSSHHPLVSL ATNPKSEHEV ATTVNQNGPE NTTKKVMEQK EEESPEERNK
560 570 580 590 600
LQVPTFGVFD DDFESDRDSE TEPEEEEQPS TPKYLSLEQR EAKTNEIKKE
610 620 630 640 650
FVSDFETLLL PSGVQEFIMS SELYNSQIES KITYKRSRDM CEISRLVEVP
660 670 680 690 700
QGVNPPSPLD AFRSTQQWDV MRCSLRDIII GSERLKEDSS SIYAKILENF
710 720 730 740 750
RTLEMFSLFY NYYFAITPLE REIAYKILNE RDWKVSKDGT MWFLRQGEVK
760 770 780 790 800
FFNEICEVGD YKIFKLDDWT VIDKINFRLD YSFLQPPVDT ASEVRDVSVD
810 820 830
NNNVNDQSNV TLEQQKQEIS HGKQLLKQLK QGKISV
Length:836
Mass (Da):94,403
Last modified:February 1, 1995 - v2
Checksum:i69B76694FCC6846F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106I → N in CAA86913 (PubMed:3018676).Curated1
Sequence conflicti593K → R in AAT92916 (PubMed:17322287).Curated1
Sequence conflicti725Y → C in CAA86913 (PubMed:3018676).Curated1
Sequence conflicti827 – 836KQLKQGKISV → ETIETGKN in CAA86913 (PubMed:3018676).Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA. Translation: CAA86913.1.
X04289 Genomic DNA. Translation: CAA27837.1.
AY692897 Genomic DNA. Translation: AAT92916.1.
BK006942 Genomic DNA. Translation: DAA08510.1.
PIRiS49940.
RefSeqiNP_012226.3. NM_001179388.3.

Genome annotation databases

EnsemblFungiiYIL038C; YIL038C; YIL038C.
GeneIDi854773.
KEGGisce:YIL038C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA. Translation: CAA86913.1.
X04289 Genomic DNA. Translation: CAA27837.1.
AY692897 Genomic DNA. Translation: AAT92916.1.
BK006942 Genomic DNA. Translation: DAA08510.1.
PIRiS49940.
RefSeqiNP_012226.3. NM_001179388.3.

3D structure databases

ProteinModelPortaliP06102.
SMRiP06102.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34952. 116 interactors.
DIPiDIP-2256N.
IntActiP06102. 45 interactors.
MINTiMINT-426406.

PTM databases

iPTMnetiP06102.

Proteomic databases

MaxQBiP06102.
PRIDEiP06102.
TopDownProteomicsiP06102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL038C; YIL038C; YIL038C.
GeneIDi854773.
KEGGisce:YIL038C.

Organism-specific databases

EuPathDBiFungiDB:YIL038C.
SGDiS000001300. NOT3.

Phylogenomic databases

GeneTreeiENSGT00390000014743.
HOGENOMiHOG000113831.
InParanoidiP06102.
KOiK12580.
OMAiMAHRKLQ.
OrthoDBiEOG092C3ROC.

Enzyme and pathway databases

BioCyciYEAST:G3O-31310-MONOMER.

Miscellaneous databases

PROiP06102.

Family and domain databases

InterProiIPR012270. CCR4-NOT_su3/5.
IPR007282. NOT.
IPR007207. Not_N.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view]
PIRSFiPIRSF005290. NOT_su_3_5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNOT3_YEAST
AccessioniPrimary (citable) accession number: P06102
Secondary accession number(s): D6VVP4, Q6B233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2490 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be CDC39 (which is in fact NOT1).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.