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P06102

- NOT3_YEAST

UniProt

P06102 - NOT3_YEAST

Protein

General negative regulator of transcription subunit 3

Gene

NOT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
    2. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
    3. protein ubiquitination Source: SGD
    4. regulation of transcription from RNA polymerase II promoter Source: SGD
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31310-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    General negative regulator of transcription subunit 3
    Gene namesi
    Name:NOT3
    Ordered Locus Names:YIL038C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL038c.
    SGDiS000001300. NOT3.

    Subcellular locationi

    GO - Cellular componenti

    1. CCR4-NOT core complex Source: SGD
    2. cytoplasm Source: SGD
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 836836General negative regulator of transcription subunit 3PRO_0000198337Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei303 – 3031Phosphoserine1 Publication
    Modified residuei307 – 3071Phosphoserine1 Publication
    Modified residuei322 – 3221Phosphoserine1 Publication
    Modified residuei446 – 4461Phosphoserine1 Publication
    Modified residuei450 – 4501Phosphoserine3 Publications
    Modified residuei454 – 4541Phosphothreonine1 Publication
    Modified residuei565 – 5651Phosphoserine2 Publications
    Modified residuei569 – 5691Phosphoserine1 Publication
    Modified residuei571 – 5711Phosphothreonine1 Publication
    Modified residuei657 – 6571Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP06102.
    PaxDbiP06102.
    PeptideAtlasiP06102.

    Expressioni

    Gene expression databases

    GenevestigatoriP06102.

    Interactioni

    Subunit structurei

    Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CAF40P538293EBI-12165,EBI-28306
    CDC39P256553EBI-12165,EBI-12139

    Protein-protein interaction databases

    BioGridi34952. 115 interactions.
    DIPiDIP-2256N.
    IntActiP06102. 45 interactions.
    MINTiMINT-426406.
    STRINGi4932.YIL038C.

    Structurei

    3D structure databases

    ProteinModelPortaliP06102.
    SMRiP06102. Positions 644-785.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili36 – 6833Sequence AnalysisAdd
    BLAST
    Coiled coili119 – 19577Sequence AnalysisAdd
    BLAST
    Coiled coili255 – 29238Sequence AnalysisAdd
    BLAST
    Coiled coili803 – 83129Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CNOT2/3/5 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5665.
    GeneTreeiENSGT00390000014743.
    HOGENOMiHOG000113831.
    KOiK12580.
    OrthoDBiEOG79SF61.

    Family and domain databases

    InterProiIPR012270. CCR4-NOT_su3/5.
    IPR007282. NOT.
    IPR007207. Not_N.
    [Graphical view]
    PfamiPF04153. NOT2_3_5. 1 hit.
    PF04065. Not3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005290. NOT_su_3_5. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P06102-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAHRKLQQEV DRVFKKINEG LEIFNSYYER HESCTNNPSQ KDKLESDLKR    50
    EVKKLQRLRE QIKSWQSSPD IKDKDSLLDY RRSVEIAMEK YKAVEKASKE 100
    KAYSNISLKK SETLDPQERE RRDISEYLSQ MIDELERQYD SLQVEIDKLL 150
    LLNKKKKTSS TTNDEKKEQY KRFQARYRWH QQQMELALRL LANEELDPQD 200
    VKNVQDDINY FVESNQDPDF VEDETIYDGL NLQSNEAIAH EVAQYFASQN 250
    AEDNNTSDAN ESLQDISKLS KKEQRKLERE AKKAAKLAAK NATGAAIPVA 300
    GPSSTPSPVI PVADASKETE RSPSSSPIHN ATKPEEAVKT SIKSPRSSAD 350
    NLLPSLQKSP SSATPETPTN VHTHIHQTPN GITGATTLKP ATLPAKPAGE 400
    LKWAVAASQA VEKDRKVTSA SSTISNTSTK TPTTAAATTT SSNANSRIGS 450
    ALNTPKLSTS SLSLQPDNTG ASSSAATAAA VLAAGAAAVH QNNQAFYRNM 500
    SSSHHPLVSL ATNPKSEHEV ATTVNQNGPE NTTKKVMEQK EEESPEERNK 550
    LQVPTFGVFD DDFESDRDSE TEPEEEEQPS TPKYLSLEQR EAKTNEIKKE 600
    FVSDFETLLL PSGVQEFIMS SELYNSQIES KITYKRSRDM CEISRLVEVP 650
    QGVNPPSPLD AFRSTQQWDV MRCSLRDIII GSERLKEDSS SIYAKILENF 700
    RTLEMFSLFY NYYFAITPLE REIAYKILNE RDWKVSKDGT MWFLRQGEVK 750
    FFNEICEVGD YKIFKLDDWT VIDKINFRLD YSFLQPPVDT ASEVRDVSVD 800
    NNNVNDQSNV TLEQQKQEIS HGKQLLKQLK QGKISV 836
    Length:836
    Mass (Da):94,403
    Last modified:February 1, 1995 - v2
    Checksum:i69B76694FCC6846F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti106 – 1061I → N in CAA86913. (PubMed:3018676)Curated
    Sequence conflicti593 – 5931K → R in AAT92916. (PubMed:17322287)Curated
    Sequence conflicti725 – 7251Y → C in CAA86913. (PubMed:3018676)Curated
    Sequence conflicti827 – 83610KQLKQGKISV → ETIETGKN in CAA86913. (PubMed:3018676)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46861 Genomic DNA. Translation: CAA86913.1.
    X04289 Genomic DNA. Translation: CAA27837.1.
    AY692897 Genomic DNA. Translation: AAT92916.1.
    BK006942 Genomic DNA. Translation: DAA08510.1.
    PIRiS49940.
    RefSeqiNP_012226.3. NM_001179388.3.

    Genome annotation databases

    EnsemblFungiiYIL038C; YIL038C; YIL038C.
    GeneIDi854773.
    KEGGisce:YIL038C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46861 Genomic DNA. Translation: CAA86913.1 .
    X04289 Genomic DNA. Translation: CAA27837.1 .
    AY692897 Genomic DNA. Translation: AAT92916.1 .
    BK006942 Genomic DNA. Translation: DAA08510.1 .
    PIRi S49940.
    RefSeqi NP_012226.3. NM_001179388.3.

    3D structure databases

    ProteinModelPortali P06102.
    SMRi P06102. Positions 644-785.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34952. 115 interactions.
    DIPi DIP-2256N.
    IntActi P06102. 45 interactions.
    MINTi MINT-426406.
    STRINGi 4932.YIL038C.

    Proteomic databases

    MaxQBi P06102.
    PaxDbi P06102.
    PeptideAtlasi P06102.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL038C ; YIL038C ; YIL038C .
    GeneIDi 854773.
    KEGGi sce:YIL038C.

    Organism-specific databases

    CYGDi YIL038c.
    SGDi S000001300. NOT3.

    Phylogenomic databases

    eggNOGi COG5665.
    GeneTreei ENSGT00390000014743.
    HOGENOMi HOG000113831.
    KOi K12580.
    OrthoDBi EOG79SF61.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31310-MONOMER.

    Miscellaneous databases

    NextBioi 977541.
    PROi P06102.

    Gene expression databases

    Genevestigatori P06102.

    Family and domain databases

    InterProi IPR012270. CCR4-NOT_su3/5.
    IPR007282. NOT.
    IPR007207. Not_N.
    [Graphical view ]
    Pfami PF04153. NOT2_3_5. 1 hit.
    PF04065. Not3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005290. NOT_su_3_5. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products."
      Ferguson J., Ho J.-Y., Peterson T.A., Reed S.I.
      Nucleic Acids Res. 14:6681-6697(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization."
      Collart M.A., Struhl K.
      Genes Dev. 8:525-537(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively."
      Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L.
      EMBO J. 17:1096-1106(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
    7. "Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
      Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
      J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450; THR-454 AND SER-657, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450; SER-565 AND SER-657, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303; SER-307; SER-446; SER-450; SER-565; SER-569 AND THR-571, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNOT3_YEAST
    AccessioniPrimary (citable) accession number: P06102
    Secondary accession number(s): D6VVP4, Q6B233
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2490 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was originally thought to be CDC39 (which is in fact NOT1).1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3