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P06102

- NOT3_YEAST

UniProt

P06102 - NOT3_YEAST

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Protein
General negative regulator of transcription subunit 3
Gene
NOT3, YIL038C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
  2. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  3. protein ubiquitination Source: SGD
  4. regulation of transcription from RNA polymerase II promoter Source: SGD
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31310-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 3
Gene namesi
Name:NOT3
Ordered Locus Names:YIL038C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL038c.
SGDiS000001300. NOT3.

Subcellular locationi

Cytoplasm Inferred. Nucleus

GO - Cellular componenti

  1. CCR4-NOT core complex Source: SGD
  2. cytoplasm Source: SGD
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 836836General negative regulator of transcription subunit 3
PRO_0000198337Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei303 – 3031Phosphoserine1 Publication
Modified residuei307 – 3071Phosphoserine1 Publication
Modified residuei322 – 3221Phosphoserine1 Publication
Modified residuei446 – 4461Phosphoserine1 Publication
Modified residuei450 – 4501Phosphoserine3 Publications
Modified residuei454 – 4541Phosphothreonine1 Publication
Modified residuei565 – 5651Phosphoserine2 Publications
Modified residuei569 – 5691Phosphoserine1 Publication
Modified residuei571 – 5711Phosphothreonine1 Publication
Modified residuei657 – 6571Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06102.
PaxDbiP06102.
PeptideAtlasiP06102.

Expressioni

Gene expression databases

GenevestigatoriP06102.

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAF40P538293EBI-12165,EBI-28306
CDC39P256553EBI-12165,EBI-12139

Protein-protein interaction databases

BioGridi34952. 115 interactions.
DIPiDIP-2256N.
IntActiP06102. 45 interactions.
MINTiMINT-426406.
STRINGi4932.YIL038C.

Structurei

3D structure databases

ProteinModelPortaliP06102.
SMRiP06102. Positions 644-785.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili36 – 6833 Reviewed prediction
Add
BLAST
Coiled coili119 – 19577 Reviewed prediction
Add
BLAST
Coiled coili255 – 29238 Reviewed prediction
Add
BLAST
Coiled coili803 – 83129 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5665.
GeneTreeiENSGT00390000014743.
HOGENOMiHOG000113831.
KOiK12580.
OrthoDBiEOG79SF61.

Family and domain databases

InterProiIPR012270. CCR4-NOT_su3/5.
IPR007282. NOT.
IPR007207. Not_N.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view]
PIRSFiPIRSF005290. NOT_su_3_5. 1 hit.

Sequencei

Sequence statusi: Complete.

P06102-1 [UniParc]FASTAAdd to Basket

« Hide

MAHRKLQQEV DRVFKKINEG LEIFNSYYER HESCTNNPSQ KDKLESDLKR    50
EVKKLQRLRE QIKSWQSSPD IKDKDSLLDY RRSVEIAMEK YKAVEKASKE 100
KAYSNISLKK SETLDPQERE RRDISEYLSQ MIDELERQYD SLQVEIDKLL 150
LLNKKKKTSS TTNDEKKEQY KRFQARYRWH QQQMELALRL LANEELDPQD 200
VKNVQDDINY FVESNQDPDF VEDETIYDGL NLQSNEAIAH EVAQYFASQN 250
AEDNNTSDAN ESLQDISKLS KKEQRKLERE AKKAAKLAAK NATGAAIPVA 300
GPSSTPSPVI PVADASKETE RSPSSSPIHN ATKPEEAVKT SIKSPRSSAD 350
NLLPSLQKSP SSATPETPTN VHTHIHQTPN GITGATTLKP ATLPAKPAGE 400
LKWAVAASQA VEKDRKVTSA SSTISNTSTK TPTTAAATTT SSNANSRIGS 450
ALNTPKLSTS SLSLQPDNTG ASSSAATAAA VLAAGAAAVH QNNQAFYRNM 500
SSSHHPLVSL ATNPKSEHEV ATTVNQNGPE NTTKKVMEQK EEESPEERNK 550
LQVPTFGVFD DDFESDRDSE TEPEEEEQPS TPKYLSLEQR EAKTNEIKKE 600
FVSDFETLLL PSGVQEFIMS SELYNSQIES KITYKRSRDM CEISRLVEVP 650
QGVNPPSPLD AFRSTQQWDV MRCSLRDIII GSERLKEDSS SIYAKILENF 700
RTLEMFSLFY NYYFAITPLE REIAYKILNE RDWKVSKDGT MWFLRQGEVK 750
FFNEICEVGD YKIFKLDDWT VIDKINFRLD YSFLQPPVDT ASEVRDVSVD 800
NNNVNDQSNV TLEQQKQEIS HGKQLLKQLK QGKISV 836
Length:836
Mass (Da):94,403
Last modified:February 1, 1995 - v2
Checksum:i69B76694FCC6846F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061I → N in CAA86913. 1 Publication
Sequence conflicti593 – 5931K → R in AAT92916. 1 Publication
Sequence conflicti725 – 7251Y → C in CAA86913. 1 Publication
Sequence conflicti827 – 83610KQLKQGKISV → ETIETGKN in CAA86913. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46861 Genomic DNA. Translation: CAA86913.1.
X04289 Genomic DNA. Translation: CAA27837.1.
AY692897 Genomic DNA. Translation: AAT92916.1.
BK006942 Genomic DNA. Translation: DAA08510.1.
PIRiS49940.
RefSeqiNP_012226.3. NM_001179388.3.

Genome annotation databases

EnsemblFungiiYIL038C; YIL038C; YIL038C.
GeneIDi854773.
KEGGisce:YIL038C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46861 Genomic DNA. Translation: CAA86913.1 .
X04289 Genomic DNA. Translation: CAA27837.1 .
AY692897 Genomic DNA. Translation: AAT92916.1 .
BK006942 Genomic DNA. Translation: DAA08510.1 .
PIRi S49940.
RefSeqi NP_012226.3. NM_001179388.3.

3D structure databases

ProteinModelPortali P06102.
SMRi P06102. Positions 644-785.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34952. 115 interactions.
DIPi DIP-2256N.
IntActi P06102. 45 interactions.
MINTi MINT-426406.
STRINGi 4932.YIL038C.

Proteomic databases

MaxQBi P06102.
PaxDbi P06102.
PeptideAtlasi P06102.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL038C ; YIL038C ; YIL038C .
GeneIDi 854773.
KEGGi sce:YIL038C.

Organism-specific databases

CYGDi YIL038c.
SGDi S000001300. NOT3.

Phylogenomic databases

eggNOGi COG5665.
GeneTreei ENSGT00390000014743.
HOGENOMi HOG000113831.
KOi K12580.
OrthoDBi EOG79SF61.

Enzyme and pathway databases

BioCyci YEAST:G3O-31310-MONOMER.

Miscellaneous databases

NextBioi 977541.
PROi P06102.

Gene expression databases

Genevestigatori P06102.

Family and domain databases

InterProi IPR012270. CCR4-NOT_su3/5.
IPR007282. NOT.
IPR007207. Not_N.
[Graphical view ]
Pfami PF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view ]
PIRSFi PIRSF005290. NOT_su_3_5. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products."
    Ferguson J., Ho J.-Y., Peterson T.A., Reed S.I.
    Nucleic Acids Res. 14:6681-6697(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization."
    Collart M.A., Struhl K.
    Genes Dev. 8:525-537(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively."
    Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L.
    EMBO J. 17:1096-1106(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
  7. "Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
    Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
    J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450; THR-454 AND SER-657, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450; SER-565 AND SER-657, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303; SER-307; SER-446; SER-450; SER-565; SER-569 AND THR-571, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNOT3_YEAST
AccessioniPrimary (citable) accession number: P06102
Secondary accession number(s): D6VVP4, Q6B233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2490 molecules/cell in log phase SD medium.

Caution

Was originally (1 Publication) thought to be CDC39 (which is in fact NOT1).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi