P06100 (NOT2_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 106.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: General negative regulator of transcription subunit 2 Alternative name(s): cell division cycle protein 36 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 191 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. NOT2 is required for the integrity of the complex. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. Ref.7 |
| Subunit structure | Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT5. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. Ref.8 Ref.11 |
| Subcellular location | |
| Miscellaneous | Present with 2120 molecules/cell in log phase SD medium. Ref.12 |
| Sequence similarities | Belongs to the CNOT2/3/5 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Cytoplasm Nucleus |
| Molecular function | Activator Repressor |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | negative regulation of transcription from RNA polymerase II promoter Traceable author statement Ref.6. Source: SGD nuclear-transcribed mRNA poly(A) tail shorteningInferred from direct assay Ref.10. Source: SGD protein ubiquitinationInferred from mutant phenotype. Source: SGD regulation of cell cycleInferred from mutant phenotype. Source: SGD response to pheromone involved in conjugation with cellular fusionInferred from mutant phenotype. Source: SGD transcription elongation from RNA polymerase II promoterInferred from mutant phenotype. Source: SGD |
| Cellular component | CCR4-NOT core complex Inferred from physical interaction Ref.8. Source: SGD cytoplasmInferred from direct assay Ref.10. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 191 | 191 | General negative regulator of transcription subunit 2 | PRO_0000198336 | |||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products." Ferguson J., Ho J.-Y., Peterson T.A., Reed S.I. Nucleic Acids Res. 14:6681-6697(1986) [PubMed: 3018676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of the DNA ligase gene (CDC9) from Saccharomyces cerevisiae: a gene which is cell-cycle regulated and induced in response to DNA damage." Barker D.G., White J.H.M., Johnston L.H. Nucleic Acids Res. 13:8323-8337(1985) [PubMed: 3909103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P.Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [5] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [6] | "NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization." Collart M.A., Struhl K. Genes Dev. 8:525-537(1994) [PubMed: 7926748] [Abstract] Cited for: CHARACTERIZATION. |
| [7] | "The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively." Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L. EMBO J. 17:1096-1106(1998) [PubMed: 9463387] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION. |
| [8] | "The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5." Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L. Mol. Cell. Biol. 19:6642-6651(1999) [PubMed: 10490603] [Abstract] Cited for: INTERACTION WITH NOT1. |
| [9] | "Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex." Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L. J. Mol. Biol. 314:683-694(2001) [PubMed: 11733989] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX. |
| [10] | "Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae." Tucker M., Staples R.R., Valencia-Sanchez M.A., Muhlrad D., Parker R. EMBO J. 21:1427-1436(2002) [PubMed: 11889048] [Abstract] Cited for: SUBCELLULAR LOCATION. |
| [11] | "Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex." Russell P., Benson J.D., Denis C.L. J. Mol. Biol. 322:27-39(2002) [PubMed: 12215412] [Abstract] Cited for: INTERACTION WITH NOT5. |
| [12] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X04287 Genomic DNA. Translation: CAA27835.1. X03246 Genomic DNA. Translation: CAA27006.1. Z67750 Genomic DNA. Translation: CAA91581.1. Z74213 Genomic DNA. Translation: CAA98739.1. X68020 Genomic DNA. Translation: CAA48160.1. AY557644 Genomic DNA. Translation: AAS55970.1. BK006938 Genomic DNA. Translation: DAA11696.1. |
| PIR | RGBY36. S12304. |
| RefSeq | NP_010116.1. NM_001180225.1. |
3D structure databases | |
| ProteinModelPortal | P06100. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-590N. |
| IntAct | P06100. 27 interactions. |
| MINT | MINT-400720. |
| STRING | P06100. |
Proteomic databases | |
| PeptideAtlas | P06100. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YDL165W; YDL165W; YDL165W. |
| GeneID | 851389. |
| KEGG | sce:YDL165W. |
| NMPDR | fig|4932.3.peg.850. |
Organism-specific databases | |
| CYGD | YDL165w. |
| SGD | S000002324. CDC36. |
Phylogenomic databases | |
| eggNOG | fuNOG10562. |
| GeneTree | EFGT00050000002523. |
| HOGENOM | HBG395524. |
| OMA | WAETSRS. |
| OrthoDB | EOG4TB7MJ. |
Gene expression databases | |
| ArrayExpress | P06100. |
| Genevestigator | P06100. |
| GermOnline | YDL165W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR007282. NOT. [Graphical view] |
| KO | K12605. |
| Pfam | PF04153. NOT2_3_5. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 968543. |
Entry information
| Entry name | NOT2_YEAST | ||||||||
| Accession | Primary (citable) accession number: P06100 Secondary accession number(s): D6VRI6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| SIMILARITY comments Index of protein domains and families |

Clusters with