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Protein

General negative regulator of transcription subunit 2

Gene

CDC36

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. NOT2 is required for the integrity of the complex. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

GO - Biological processi

  • deadenylation-independent decapping of nuclear-transcribed mRNA Source: SGD
  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: SGD
  • nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • protein ubiquitination Source: SGD
  • regulation of cell cycle Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • response to pheromone involved in conjugation with cellular fusion Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29557-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 2
Alternative name(s):
cell division cycle protein 36
Gene namesi
Name:CDC36
Synonyms:DNA19, NOT2
Ordered Locus Names:YDL165W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL165W.
SGDiS000002324. CDC36.

Subcellular locationi

GO - Cellular componenti

  • CCR4-NOT core complex Source: SGD
  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191General negative regulator of transcription subunit 2PRO_0000198336Add
BLAST

Proteomic databases

MaxQBiP06100.
PeptideAtlasiP06100.

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT5. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.4 Publications

Protein-protein interaction databases

BioGridi31900. 97 interactions.
DIPiDIP-590N.
IntActiP06100. 24 interactions.
MINTiMINT-400720.

Structurei

Secondary structure

1
191
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Helixi35 – 406Combined sources
Helixi53 – 553Combined sources
Beta strandi59 – 613Combined sources
Helixi76 – 783Combined sources
Helixi84 – 863Combined sources
Beta strandi88 – 914Combined sources
Turni97 – 993Combined sources
Helixi101 – 1044Combined sources
Helixi109 – 11810Combined sources
Helixi123 – 13412Combined sources
Beta strandi138 – 1403Combined sources
Turni141 – 1444Combined sources
Beta strandi145 – 1495Combined sources
Beta strandi161 – 17212Combined sources
Turni173 – 1764Combined sources
Beta strandi177 – 18610Combined sources
Helixi187 – 1893Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BY6X-ray2.80B/E1-191[»]
ProteinModelPortaliP06100.
SMRiP06100. Positions 4-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000001285.
HOGENOMiHOG000246523.
InParanoidiP06100.
KOiK12605.
OMAiVARNWRY.
OrthoDBiEOG7WQ84Q.

Family and domain databases

InterProiIPR007282. NOT.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKFGLKALV PLLKLEDKEL SSTYDHSMTL GADLSSMLYS LGIPRDSQDH
60 70 80 90 100
RVLDTFQSPW AETSRSEVEP RFFTPESFTN IPGVLQSTVT PPCFNSIQND
110 120 130 140 150
QQRVALFQDE TLFFLFYKHP GTVIQELTYL ELRKRNWRYH KTLKAWLTKD
160 170 180 190
PMMEPIVSAD GLSERGSYVF FDPQRWEKCQ RDFLLFYNAI M
Length:191
Mass (Da):22,363
Last modified:January 1, 1988 - v1
Checksum:i7D0B69657F791371
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04287 Genomic DNA. Translation: CAA27835.1.
X03246 Genomic DNA. Translation: CAA27006.1.
Z67750 Genomic DNA. Translation: CAA91581.1.
Z74213 Genomic DNA. Translation: CAA98739.1.
X68020 Genomic DNA. Translation: CAA48160.1.
AY557644 Genomic DNA. Translation: AAS55970.1.
BK006938 Genomic DNA. Translation: DAA11696.1.
PIRiS12304. RGBY36.
RefSeqiNP_010116.1. NM_001180225.1.

Genome annotation databases

EnsemblFungiiYDL165W; YDL165W; YDL165W.
GeneIDi851389.
KEGGisce:YDL165W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04287 Genomic DNA. Translation: CAA27835.1.
X03246 Genomic DNA. Translation: CAA27006.1.
Z67750 Genomic DNA. Translation: CAA91581.1.
Z74213 Genomic DNA. Translation: CAA98739.1.
X68020 Genomic DNA. Translation: CAA48160.1.
AY557644 Genomic DNA. Translation: AAS55970.1.
BK006938 Genomic DNA. Translation: DAA11696.1.
PIRiS12304. RGBY36.
RefSeqiNP_010116.1. NM_001180225.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BY6X-ray2.80B/E1-191[»]
ProteinModelPortaliP06100.
SMRiP06100. Positions 4-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31900. 97 interactions.
DIPiDIP-590N.
IntActiP06100. 24 interactions.
MINTiMINT-400720.

Proteomic databases

MaxQBiP06100.
PeptideAtlasiP06100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL165W; YDL165W; YDL165W.
GeneIDi851389.
KEGGisce:YDL165W.

Organism-specific databases

EuPathDBiFungiDB:YDL165W.
SGDiS000002324. CDC36.

Phylogenomic databases

GeneTreeiENSGT00390000001285.
HOGENOMiHOG000246523.
InParanoidiP06100.
KOiK12605.
OMAiVARNWRY.
OrthoDBiEOG7WQ84Q.

Enzyme and pathway databases

BioCyciYEAST:G3O-29557-MONOMER.

Miscellaneous databases

PROiP06100.

Family and domain databases

InterProiIPR007282. NOT.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products."
    Ferguson J., Ho J.-Y., Peterson T.A., Reed S.I.
    Nucleic Acids Res. 14:6681-6697(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of the DNA ligase gene (CDC9) from Saccharomyces cerevisiae: a gene which is cell-cycle regulated and induced in response to DNA damage."
    Barker D.G., White J.H.M., Johnston L.H.
    Nucleic Acids Res. 13:8323-8337(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization."
    Collart M.A., Struhl K.
    Genes Dev. 8:525-537(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively."
    Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L.
    EMBO J. 17:1096-1106(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
  8. "The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5."
    Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L.
    Mol. Cell. Biol. 19:6642-6651(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOT1.
  9. "Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
    Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
    J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
  10. "Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae."
    Tucker M., Staples R.R., Valencia-Sanchez M.A., Muhlrad D., Parker R.
    EMBO J. 21:1427-1436(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  11. "Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex."
    Russell P., Benson J.D., Denis C.L.
    J. Mol. Biol. 322:27-39(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOT5.
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNOT2_YEAST
AccessioniPrimary (citable) accession number: P06100
Secondary accession number(s): D6VRI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 8, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2120 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.