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Reviewed, UniProtKB/Swiss-Prot P06100 (NOT2_YEAST)

Last modified November 24, 2009. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    General negative regulator of transcription subunit 2
Gene names
Name: NOT2
Synonyms: CDC36, DNA19
Ordered Locus Names: YDL165W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length191 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. NOT2 is required for the integrity of the complex. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. Ref.6

Subunit structure

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT5. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. Ref.7 Ref.10

Subcellular location

Cytoplasm. Nucleus.

Miscellaneous

Present with 2120 molecules/cell in log phase SD medium. Ref.11

Sequence similarities

Belongs to the CNOT2/3/5 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 191191General negative regulator of transcription subunit 2
PRO_0000198336

Sequences

Sequence LengthMass (Da)Tools
P06100-1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 7D0B69657F791371

FASTA19122,363
        10         20         30         40         50         60 
MEKFGLKALV PLLKLEDKEL SSTYDHSMTL GADLSSMLYS LGIPRDSQDH RVLDTFQSPW 

        70         80         90        100        110        120 
AETSRSEVEP RFFTPESFTN IPGVLQSTVT PPCFNSIQND QQRVALFQDE TLFFLFYKHP 

       130        140        150        160        170        180 
GTVIQELTYL ELRKRNWRYH KTLKAWLTKD PMMEPIVSAD GLSERGSYVF FDPQRWEKCQ 

       190 
RDFLLFYNAI M 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products."
Ferguson J., Ho J.-Y., Peterson T.A., Reed S.I.
Nucleic Acids Res. 14:6681-6697(1986) [PubMed: 3018676] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of the DNA ligase gene (CDC9) from Saccharomyces cerevisiae: a gene which is cell-cycle regulated and induced in response to DNA damage."
Barker D.G., White J.H.M., Johnston L.H.
Nucleic Acids Res. 13:8323-8337(1985) [PubMed: 3909103] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization."
Collart M.A., Struhl K.
Genes Dev. 8:525-537(1994) [PubMed: 7926748] [Abstract]
Cited for: CHARACTERIZATION.
[6]"The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively."
Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L.
EMBO J. 17:1096-1106(1998) [PubMed: 9463387] [Abstract]
Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
[7]"The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5."
Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L.
Mol. Cell. Biol. 19:6642-6651(1999) [PubMed: 10490603] [Abstract]
Cited for: INTERACTION WITH NOT1.
[8]"Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
J. Mol. Biol. 314:683-694(2001) [PubMed: 11733989] [Abstract]
Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
[9]"Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae."
Tucker M., Staples R.R., Valencia-Sanchez M.A., Muhlrad D., Parker R.
EMBO J. 21:1427-1436(2002) [PubMed: 11889048] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex."
Russell P., Benson J.D., Denis C.L.
J. Mol. Biol. 322:27-39(2002) [PubMed: 12215412] [Abstract]
Cited for: INTERACTION WITH NOT5.
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

X04287 Genomic DNA. Translation: CAA27835.1.
X03246 Genomic DNA. Translation: CAA27006.1.
Z67750 Genomic DNA. Translation: CAA91581.1.
Z74213 Genomic DNA. Translation: CAA98739.1.
X68020 Genomic DNA. Translation: CAA48160.1.
AY557644 Genomic DNA. Translation: AAS55970.1.
PIRRGBY36. S12304.
RefSeqNP_010116.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:590N.
IntActP06100. 29 interactions.
STRINGP06100.

Proteomic databases

PeptideAtlasP06100.

Genome annotation databases

EnsemblYDL165W; YDL165W; YDL165W; Saccharomyces cerevisiae. [Genome view]
GeneID851389.
KEGGsce:YDL165W.
NMPDRfig|4932.3.peg.850.

Organism-specific databases

CYGDYDL165w.
SGDS000002324. CDC36.

Phylogenomic databases

HOGENOMP06100.
OMAWAETSRS
OrthoDBEOG9RZ0GH

Gene expression databases

ArrayExpressP06100.
GenevestigatorP06100.
GermOnlineYDL165W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR007282. NOT.
[Graphical view]
PfamPF04153. NOT2_3_5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio968543.

Entry information

Entry nameNOT2_YEAST
AccessionPrimary (citable) accession number: P06100
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 24, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents