Reviewed, UniProtKB/Swiss-Prot P06100 (NOT2_YEAST)

Names and origin

Protein names

Recommended name:
    General negative regulator of transcription subunit 2

Gene names

Name:	NOT2
Synonyms:	CDC36, DNA19
Ordered Locus Names:	YDL165W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. NOT2 is required for the integrity of the complex. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. Ref.6
Subunit structure	Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT5. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. Ref.7 Ref.10
Subcellular location	Cytoplasm. Nucleus.
Miscellaneous	Present with 2120 molecules/cell in log phase SD medium. Ref.11
Sequence similarities	Belongs to the CNOT2/3/5 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Activator Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA elongation from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD negative regulation of transcription from RNA polymerase II promoter Ref.5 Traceable author statement. Source: SGD nuclear-transcribed mRNA poly(A) tail shortening Ref.9 Inferred from direct assay. Source: SGD protein ubiquitination Inferred from mutant phenotype. Source: SGD regulation of cell cycle Inferred from mutant phenotype. Source: SGD response to pheromone during conjugation with cellular fusion Inferred from mutant phenotype. Source: SGD
Cellular component	CCR4-NOT core complex Ref.7 Inferred from physical interaction. Source: SGD cytoplasm Ref.9 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAF40	P53829	1	EBI-12153,EBI-28306
DID4	P36108	1	EBI-12153,EBI-26574
NOT1	P25655	1	EBI-12153,EBI-12139
NOT3	P06102	1	EBI-12153,EBI-12165
NOT4	P34909	1	EBI-12153,EBI-12174
PAT1	P25644	1	EBI-12153,EBI-204
PIN4	P34217	1	EBI-12153,EBI-21256
TAF2	P23255	1	EBI-12153,EBI-18862

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	General negative regulator of transcription subunit 2		PRO_0000198336

Sequences

	Sequence		Length	Mass (Da)
	P06100-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 7D0B69657F791371	FASTA	191	22,363
	10 20 30 40 50 60 MEKFGLKALV PLLKLEDKEL SSTYDHSMTL GADLSSMLYS LGIPRDSQDH RVLDTFQSPW 70 80 90 100 110 120 AETSRSEVEP RFFTPESFTN IPGVLQSTVT PPCFNSIQND QQRVALFQDE TLFFLFYKHP 130 140 150 160 170 180 GTVIQELTYL ELRKRNWRYH KTLKAWLTKD PMMEPIVSAD GLSERGSYVF FDPQRWEKCQ 190 RDFLLFYNAI M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products." Ferguson J., Ho J.-Y., Peterson T.A., Reed S.I. Nucleic Acids Res. 14:6681-6697(1986) [PubMed: 3018676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of the DNA ligase gene (CDC9) from Saccharomyces cerevisiae: a gene which is cell-cycle regulated and induced in response to DNA damage." Barker D.G., White J.H.M., Johnston L.H. Nucleic Acids Res. 13:8323-8337(1985) [PubMed: 3909103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization." Collart M.A., Struhl K. Genes Dev. 8:525-537(1994) [PubMed: 7926748] [Abstract] Cited for: CHARACTERIZATION.
[6]	"The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively." Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L. EMBO J. 17:1096-1106(1998) [PubMed: 9463387] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
[7]	"The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5." Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L. Mol. Cell. Biol. 19:6642-6651(1999) [PubMed: 10490603] [Abstract] Cited for: INTERACTION WITH NOT1.
[8]	"Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex." Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L. J. Mol. Biol. 314:683-694(2001) [PubMed: 11733989] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
[9]	"Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae." Tucker M., Staples R.R., Valencia-Sanchez M.A., Muhlrad D., Parker R. EMBO J. 21:1427-1436(2002) [PubMed: 11889048] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex." Russell P., Benson J.D., Denis C.L. J. Mol. Biol. 322:27-39(2002) [PubMed: 12215412] [Abstract] Cited for: INTERACTION WITH NOT5.
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04287 Genomic DNA. Translation: CAA27835.1. X03246 Genomic DNA. Translation: CAA27006.1. Z67750 Genomic DNA. Translation: CAA91581.1. Z74213 Genomic DNA. Translation: CAA98739.1. X68020 Genomic DNA. Translation: CAA48160.1. AY557644 Genomic DNA. Translation: AAS55970.1.
PIR	RGBY36. S12304.
RefSeq	NP_010116.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:590N.
IntAct	P06100. 29 interactions.
STRING	P06100.
Proteomic databases
PeptideAtlas	P06100.
Genome annotation databases
Ensembl	YDL165W; YDL165W; YDL165W; Saccharomyces cerevisiae. [Genome view]
GeneID	851389.
KEGG	sce:YDL165W.
NMPDR	fig|4932.3.peg.850.
Organism-specific databases
CYGD	YDL165w.
SGD	S000002324. CDC36.
Phylogenomic databases
HOGENOM	P06100.
OMA	WAETSRS
OrthoDB	EOG9RZ0GH
Gene expression databases
ArrayExpress	P06100.
Genevestigator	P06100.
GermOnline	YDL165W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007282. NOT. [Graphical view]
Pfam	PF04153. NOT2_3_5. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968543.

Entry information

Entry name

NOT2_YEAST

Accession

Primary (citable) accession number: P06100

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	November 24, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)