P06030 (COX3_PARDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 3
EC=1.9.3.1

Alternative name(s):
Cytochrome aa3 subunit 3
Cytochrome c oxidase polypeptide III
Oxidase aa(3) subunit 3

Gene names

Name:	ctaE
Synonyms:	coiII

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 3 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	aerobic electron transport chain Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 274

273

Cytochrome c oxidase subunit 3

PRO_0000183886

Regions

Topological domain

2 – 15

14

Cytoplasmic

Transmembrane

16 – 36

21

Helical

Topological domain

37 – 48

12

Periplasmic

Transmembrane

49 – 77

29

Helical

Topological domain

78 – 79

2

Cytoplasmic

Transmembrane

80 – 115

36

Helical

Topological domain

116 – 139

24

Periplasmic

Transmembrane

140 – 166

27

Helical

Topological domain

167 – 168

2

Cytoplasmic

Transmembrane

169 – 197

29

Helical

Topological domain

198 – 203

6

Periplasmic

Transmembrane

204 – 237

34

Helical

Topological domain

238 – 244

7

Cytoplasmic

Transmembrane

245 – 274

30

Helical

Secondary structure

1

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274

Helix Strand Turn

Details...

Helix

17 – 36

20

Beta strand

42 – 44

3

Turn

51 – 53

3

Helix

54 – 75

22

Helix

81 – 114

34

Turn

119 – 123

5

Turn

138 – 140

3

Helix

141 – 165

25

Helix

169 – 175

7

Helix

177 – 180

4

Helix

182 – 193

12

Helix

194 – 196

3

Beta strand

201 – 203

3

Helix

205 – 235

31

Turn

236 – 238

3

Helix

246 – 267

22

Helix

268 – 272

5

Sequences

	Sequence		Length	Mass (Da)
	P06030 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 082F95AF9AD4A27B	FASTA	274	30,786
	10 20 30 40 50 60 MAHVKNHDYQ ILPPSIWPFF GAIGAFVMLT GAVAWMKGIT FFGLPVEGPW MFLIGLVGVL 70 80 90 100 110 120 YVMFGWWADV VNEGETGEHT PVVRIGLQYG FILFIMSEVM FFVAWFWAFI KNALYPMGPD 130 140 150 160 170 180 SPIKDGVWPP EGIVTFDPWH LPLINTLILL LSGVAVTWAH HAFVLEGDRK TTINGLIVAV 190 200 210 220 230 240 ILGVCFTGLQ AYEYSHAAFG LADTVYAGAF YMATGFHGAH VIIGTIFLFV CLIRLLKGQM 250 260 270 TQKQHVGFEA AAWYWHFVDV VWLFLFVVIY IWGR

References

[1]	"Isolation and analysis of the genes for cytochrome c oxidase in Paracoccus denitrificans." Raitio M., Jalli T., Saraste M. EMBO J. 6:2825-2833(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S1657.
[2]	"A gene in Paracoccus for subunit III of cytochrome oxidase." Saraste M., Raitio M., Jalli T., Peraemaa A. FEBS Lett. 206:154-156(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-144. Strain: S1657.
[3]	"The Paracoccus denitrificans cytochrome aa3 has a third subunit." Haltia T., Puustinen A., Finel M. Eur. J. Biochem. 172:543-546(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16.
[4]	"Structure at 2.8-A resolution of cytochrome c oxidase from Paracoccus denitrificans." Iwata S., Ostermeier C., Ludwig B., Michel H. Nature 376:660-669(1995) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X04406 Genomic DNA. Translation: CAA27995.1.
X05828 Genomic DNA. Translation: CAA29272.1.

PIR

S03807.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QLE	X-ray	3.00	C	2-273	[»]

ProteinModelPortal

P06030.

SMR

P06030. Positions 2-274.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.20.120.80. 1 hit.

InterPro

IPR024791. Cyt_c/ubiquinol_Oxase_su3.
IPR000298. Cyt_c_oxidase_su3.
IPR013833. Cyt_c_oxidase_su3_a-hlx.
[Graphical view]

PANTHER

PTHR11403. PTHR11403. 1 hit.

Pfam

PF00510. COX3. 1 hit.
[Graphical view]

SUPFAM

SSF81452. CytC_oxdse_III. 1 hit.

PROSITE

PS50253. COX3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P06030.

Entry information

Entry name

COX3_PARDE

Accession

Primary (citable) accession number: P06030

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program