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P06028 (GLPB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycophorin-B
Alternative name(s):
PAS-3
SS-active sialoglycoprotein
Sialoglycoprotein delta
CD_antigen=CD235b
Gene names
Name:GYPB
Synonyms:GPB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length91 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is a minor sialoglycoprotein in erythrocyte membranes.

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Post-translational modification

The N-terminal extracellular domain is heavily glycosylated on serine and threonine residues. Ref.13

Polymorphism

Along with GYPA, GYPB is responsible for the MNS blood group system. The molecular basis of the S/s blood group antigen is a single variation in position 48; Thr-48 corresponds to s=MSN4 and Met-48 to S=MNS3.

Sequence similarities

Belongs to the glycophorin-A family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P06028-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P06028-2)

The sequence of this isoform differs from the canonical sequence as follows:
     13-45: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.10 Ref.11 Ref.13
Chain20 – 9172Glycophorin-B
PRO_0000012136

Regions

Topological domain20 – 5940Extracellular Potential
Transmembrane60 – 8122Helical; Potential
Topological domain82 – 9110Cytoplasmic Potential

Sites

Site331Not glycosylated
Site341Not glycosylated

Amino acid modifications

Glycosylation361O-linked (GalNAc...) Ref.13
Glycosylation381O-linked (GalNAc...) Ref.13

Natural variations

Alternative sequence13 – 4533Missing in isoform 2.
VSP_047824
Natural variant221T → S in M(v) antigen. Ref.7
VAR_047948
Natural variant481T → M in S antigen and Mit antigen. Ref.7
Corresponds to variant rs7683365 [ dbSNP | Ensembl ].
VAR_003192
Natural variant541R → H in Mit antigen. Ref.7
VAR_047949
Natural variant581P → R in s(D) antigen. Ref.7
VAR_047950
Natural variant841S → T. Ref.1 Ref.3 Ref.7
Corresponds to variant rs1132783 [ dbSNP | Ensembl ].
VAR_030785

Experimental info

Sequence conflict691C → S AA sequence Ref.10
Sequence conflict691C → S AA sequence Ref.11

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 21, 2012. Version 3.
Checksum: EF15A64BAA929B7B

FASTA919,782
        10         20         30         40         50         60 
MYGKIIFVLL LSEIVSISAL STTEVAMHTS TSSSVTKSYI SSQTNGETGQ LVHRFTVPAP 

        70         80         90 
VVIILIILCV MAGIIGTILL ISYSIRRLIK A 

« Hide

Isoform 2 [UniParc].

Checksum: 1DE98AE83FBB43A2
Show »

FASTA586,352

References

« Hide 'large scale' references
[1]"Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A."
Siebert P.D., Fukuda M.
Proc. Natl. Acad. Sci. U.S.A. 84:6735-6739(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT THR-84.
[2]"Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta."
Tate C.G., Tanner M.J.A.
Biochem. J. 254:743-750(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences."
Kudo S., Fukuda M.
Proc. Natl. Acad. Sci. U.S.A. 86:4619-4623(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-84.
[4]"Extensive alternative splicing of glycophorins in Southeast Asian populations."
Hsu K., Chi N., Lin M.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
Tissue: Blood.
[5]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The low-incidence MNS antigens M(v), s(D), and Mit arise from single amino acid substitutions on GPB."
Storry J.R., Reid M.E., MacLennan S., Lubenko A., Nortman P.
Transfusion 41:269-275(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT M(V) SER-22, VARIANTS MIT MET-48 AND HIS-54, VARIANT S(D) ARG-58, VARIANT THR-84.
Tissue: Blood.
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[9]"Molecular genetics of human erythrocyte MiIII and MiVI glycophorins. Use of a pseudoexon in construction of two delta-alpha-delta hybrid genes resulting in antigenic diversification."
Huang C.-H., Blumenfeld O.O.
J. Biol. Chem. 266:7248-7255(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-58.
Tissue: Blood.
[10]"Hybrid glycophorins from human erythrocyte membranes. I. Isolation and complete structural analysis of the hybrid sialoglycoprotein from Dantu-positive red cells of the N.E. variety."
Dahr W., Beyreuther K., Moulds J., Unger P.
Eur. J. Biochem. 166:31-36(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-90.
[11]"Glycophorins B and C from human erythrocyte membranes. Purification and sequence analysis."
Blanchard D., Dahr W., Hummel M., Latron F., Beyreuther K., Cartron J.-P.
J. Biol. Chem. 262:5808-5811(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-90.
[12]"Molecular basis for the human erythrocyte glycophorin specifying the Miltenberger class I (MiI) phenotype."
Huang C.-H., Spruell P., Moulds J.J., Blumenfeld O.O.
Blood 80:257-263(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-45.
[13]"Epitopic structure of Tn glycophorin A for an anti-Tn antibody (MLS 128)."
Nakada H., Inoue M., Numata Y., Tanaka N., Funakoshi I., Fukui S., Mellors A., Yamashina I.
Proc. Natl. Acad. Sci. U.S.A. 90:2495-2499(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-40, GLYCOSYLATION AT THR-36 AND SER-38.
+Additional computationally mapped references.

Web resources

dbRBC/BGMUT

Blood group antigen gene mutation database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02982 mRNA. Translation: AAA52573.1.
X08055 mRNA. Translation: CAB42645.1.
M24137 expand/collapse EMBL AC list , M24130, M24131, M24135 Genomic DNA. Translation: AAA58626.1.
AY950609 mRNA. Translation: AAX53130.1.
AY950610 mRNA. Translation: AAX53131.1.
AY950611 mRNA. Translation: AAX53132.1.
EU338223 mRNA. Translation: ACA96781.1.
EU338235 mRNA. Translation: ACA96793.1.
AC093890 Genomic DNA. No translation available.
AC107223 Genomic DNA. No translation available.
AC110762 Genomic DNA. No translation available.
CH471056 Genomic DNA. Translation: EAX05058.1.
BC069310 mRNA. Translation: AAH69310.1.
BC121077 mRNA. Translation: AAI21078.1.
BC121078 mRNA. Translation: AAI21079.1.
M60708 Genomic DNA. Translation: AAC63048.1.
CCDSCCDS54809.1. [P06028-1]
PIRB33931.
RefSeqNP_002091.3. NM_002100.4.
UniGeneHs.654368.

3D structure databases

ProteinModelPortalP06028.
SMRP06028. Positions 49-91.
ModBaseSearch...
MobiDBSearch...

PTM databases

UniCarbKBP06028.

Polymorphism databases

DMDM380865467.

Protocols and materials databases

DNASU2994.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000283126; ENSP00000283126; ENSG00000250361. [P06028-1]
ENST00000502664; ENSP00000427690; ENSG00000250361. [P06028-1]
ENST00000513128; ENSP00000425244; ENSG00000250361. [P06028-2]
GeneID2994.
KEGGhsa:2994.

Organism-specific databases

CTD2994.
GeneCardsGC04M144918.
HGNCHGNC:4703. GYPB.
MIM111740. gene+phenotype.
neXtProtNX_P06028.
PharmGKBPA29081.
GenAtlasSearch...

Phylogenomic databases

HOGENOMHOG000089933.
HOVERGENHBG005850.
KOK06575.
OMAADSKERY.
PhylomeDBP06028.
TreeFamTF338555.

Gene expression databases

ArrayExpressP06028.
BgeeP06028.
CleanExHS_GYPB.
GenevestigatorP06028.

Family and domain databases

InterProIPR001195. Glycophorin.
IPR018938. Glycophorin_CS.
[Graphical view]
PANTHERPTHR13813. PTHR13813. 1 hit.
PROSITEPS00312. GLYCOPHORIN_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGYPB.
GenomeRNAi2994.
NextBio11866.
PROP06028.
SOURCESearch...

Entry information

Entry nameGLPB_HUMAN
AccessionPrimary (citable) accession number: P06028
Secondary accession number(s): B8Q174 expand/collapse secondary AC list , E2QBW7, Q0VAF4, Q58HE9, Q58HF0, Q58HF1, Q9UCH7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: March 21, 2012
Last modified: July 9, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Blood group antigen proteins

Nomenclature of blood group antigens and list of entries