P06028 (GLPB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycophorin-B

Alternative name(s):
PAS-3
SS-active sialoglycoprotein
Sialoglycoprotein delta
CD_antigen=CD235b

Gene names

Name:	GYPB
Synonyms:	GPB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	91 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is a minor sialoglycoprotein in erythrocyte membranes.
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Post-translational modification	The N-terminal extracellular domain is heavily glycosylated on serine and threonine residues. Ref.12
Polymorphism	Along with GYPA, GYPB is responsible for the MNS blood group system. The molecular basis of the S/s blood group antigen is a single variation in position 48; Thr-48 corresponds to s=MSN4 and Met-48 to S=MNS3.
Sequence similarities	Belongs to the glycophorin-A family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Ligand	Sialic acid
Molecular function	Blood group antigen
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Traceable author statement PubMed 7622054. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.9 Ref.10 Ref.12
	Chain	20 – 91	72	Glycophorin-B		PRO_0000012136
Regions
	Topological domain	20 – 59	40	Extracellular Potential
	Transmembrane	60 – 81	22	Helical; Potential
	Topological domain	82 – 91	10	Cytoplasmic Potential
Sites
	Site	33	1	Not glycosylated
	Site	34	1	Not glycosylated
Amino acid modifications
	Glycosylation	36	1	O-linked (GlcNAc...) Ref.12
	Glycosylation	38	1	O-linked (GlcNAc...) Ref.12
Natural variations
	Natural variant	22	1	T → S in M(v) antigen. Ref.6		VAR_047948
	Natural variant	48	1	T → M in S antigen and Mit antigen. Ref.6 Corresponds to variant rs7683365 [ dbSNP | Ensembl ].		VAR_003192
	Natural variant	54	1	R → H in Mit antigen. Ref.6		VAR_047949
	Natural variant	58	1	P → R in s(D) antigen. Ref.6		VAR_047950
	Natural variant	84	1	S → T. Ref.1 Ref.3 Ref.6 Corresponds to variant rs1132783 [ dbSNP | Ensembl ].		VAR_030785
Experimental info
	Sequence conflict	69	1	C → S AA sequence Ref.9
	Sequence conflict	69	1	C → S AA sequence Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P06028 [UniParc]. Last modified March 21, 2012. Version 3. Checksum: EF15A64BAA929B7B	FASTA	91	9,782
	10 20 30 40 50 60 MYGKIIFVLL LSEIVSISAL STTEVAMHTS TSSSVTKSYI SSQTNGETGQ LVHRFTVPAP 70 80 90 VVIILIILCV MAGIIGTILL ISYSIRRLIK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A." Siebert P.D., Fukuda M. Proc. Natl. Acad. Sci. U.S.A. 84:6735-6739(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-84.
[2]	"Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta." Tate C.G., Tanner M.J.A. Biochem. J. 254:743-750(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences." Kudo S., Fukuda M. Proc. Natl. Acad. Sci. U.S.A. 86:4619-4623(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-84.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The low-incidence MNS antigens M(v), s(D), and Mit arise from single amino acid substitutions on GPB." Storry J.R., Reid M.E., MacLennan S., Lubenko A., Nortman P. Transfusion 41:269-275(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT M(V) SER-22, VARIANTS MIT MET-48 AND HIS-54, VARIANT S(D) ARG-58, VARIANT THR-84. Tissue: Blood.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Molecular genetics of human erythrocyte MiIII and MiVI glycophorins. Use of a pseudoexon in construction of two delta-alpha-delta hybrid genes resulting in antigenic diversification." Huang C.-H., Blumenfeld O.O. J. Biol. Chem. 266:7248-7255(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-58. Tissue: Blood.
[9]	"Hybrid glycophorins from human erythrocyte membranes. I. Isolation and complete structural analysis of the hybrid sialoglycoprotein from Dantu-positive red cells of the N.E. variety." Dahr W., Beyreuther K., Moulds J., Unger P. Eur. J. Biochem. 166:31-36(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-90.
[10]	"Glycophorins B and C from human erythrocyte membranes. Purification and sequence analysis." Blanchard D., Dahr W., Hummel M., Latron F., Beyreuther K., Cartron J.-P. J. Biol. Chem. 262:5808-5811(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-90.
[11]	"Molecular basis for the human erythrocyte glycophorin specifying the Miltenberger class I (MiI) phenotype." Huang C.-H., Spruell P., Moulds J.J., Blumenfeld O.O. Blood 80:257-263(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-45.
[12]	"Epitopic structure of Tn glycophorin A for an anti-Tn antibody (MLS 128)." Nakada H., Inoue M., Numata Y., Tanaka N., Funakoshi I., Fukui S., Mellors A., Yamashina I. Proc. Natl. Acad. Sci. U.S.A. 90:2495-2499(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-40, GLYCOSYLATION AT THR-36 AND SER-38.
+	Additional computationally mapped references.

Web resources

dbRBC/BGMUT

Blood group antigen gene mutation database

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02982 mRNA. Translation: AAA52573.1. X08055 mRNA. Translation: CAB42645.1. M24137 , M24130, M24131, M24135 Genomic DNA. Translation: AAA58626.1. AY950609 mRNA. Translation: AAX53130.1. AY950610 mRNA. Translation: AAX53131.1. AY950611 mRNA. Translation: AAX53132.1. AC093890 Genomic DNA. No translation available. AC107223 Genomic DNA. No translation available. AC110762 Genomic DNA. No translation available. CH471056 Genomic DNA. Translation: EAX05058.1. BC069310 mRNA. Translation: AAH69310.1. BC121077 mRNA. Translation: AAI21078.1. BC121078 mRNA. Translation: AAI21079.1. M60708 Genomic DNA. Translation: AAC63048.1.
IPI	IPI00024995.
PIR	B33931.
RefSeq	NP_002091.3. NM_002100.4.
UniGene	Hs.654368.
3D structure databases
ProteinModelPortal	P06028.
SMR	P06028. Positions 49-91.
ModBase	Search...
PTM databases
GlycoSuiteDB	P06028.
Polymorphism databases
DMDM	121405.
Protocols and materials databases
DNASU	2994.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000283126; ENSP00000283126; ENSG00000250361. ENST00000502664; ENSP00000427690; ENSG00000250361.
GeneID	2994.
KEGG	hsa:2994.
UCSC	uc003ijm.1. human.
Organism-specific databases
CTD	2994.
GeneCards	GC04M144918.
HGNC	HGNC:4703. GYPB.
MIM	111740. gene+phenotype.
neXtProt	NX_P06028.
PharmGKB	PA29081.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HOG000089933.
HOVERGEN	HBG005850.
KO	K06575.
OMA	YISSETN.
Gene expression databases
ArrayExpress	P06028.
Bgee	P06028.
CleanEx	HS_GYPB.
Genevestigator	P06028.
GermOnline	ENSG00000153143. Homo sapiens.
Family and domain databases
InterPro	IPR001195. Glycophorin. IPR018938. Glycophorin_CS. [Graphical view]
PANTHER	PTHR13813. PTHR13813. 1 hit.
Pfam	PF01102. Glycophorin_A. 1 hit. [Graphical view]
PROSITE	PS00312. GLYCOPHORIN_A. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	2994.
NextBio	11866.
SOURCE	Search...

Entry information

Entry name

GLPB_HUMAN

Accession

Primary (citable) accession number: P06028
Secondary accession number(s): E2QBW7 , Q0VAF4, Q58HE9, Q58HF0, Q58HF1, Q9UCH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	March 21, 2012
Last modified:	April 3, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.