Reviewed, UniProtKB/Swiss-Prot P06028 (GLPB_HUMAN)

Names and origin

Protein names

Recommended name:
    Glycophorin-B
Alternative name(s):
    PAS-3
    Sialoglycoprotein delta
    SS-active sialoglycoprotein
    CD_antigen=CD235b

Gene names

Name:	GYPB
Synonyms:	GPB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	91 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein is a minor sialoglycoprotein in erythrocyte membranes.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	The N-terminal extracellular domain is heavily glycosylated on serine and threonine residues By similarity.
Polymorphism	Along with GYPA, GYPB is responsible for the MNS blood group system. The molecular basis of the S/s blood group antigen is a single variation in position 48; Thr-48 corresponds to s=MSN4 and Met-48 to S=MNS3.
Sequence similarities	Belongs to the glycophorin A family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane
Ligand	Sialic acid
Molecular function	Blood group antigen
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 19	19
	Chain	20 – 91	72	Glycophorin-B
Regions
	Topological domain	20 – 59	40	Extracellular Potential
	Transmembrane	60 – 81	22	Potential
	Topological domain	82 – 91	10	Cytoplasmic Potential
Natural variations
	Natural variant	48	1	T → M in S antigen. dbSNP rs7683365.
	Natural variant	84	1	T → S: dbSNP rs1132783.
Experimental info
	Sequence conflict	69	1	C → S Ref.6 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P06028-1 [UniParc]. Last modified January 1, 1990. Version 2. Checksum: F415A64BAA929B7F	FASTA	91	9,796
	10 20 30 40 50 60 MYGKIIFVLL LSEIVSISAL STTEVAMHTS TSSSVTKSYI SSQTNGETGQ LVHRFTVPAP 70 80 90 VVIILIILCV MAGIIGTILL ISYTIRRLIK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A." Siebert P.D., Fukuda M. Proc. Natl. Acad. Sci. U.S.A. 84:6735-6739(1987) [PubMed: 3477806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta." Tate C.G., Tanner M.J.A. Biochem. J. 254:743-750(1988) [PubMed: 3196288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-84.
[3]	"Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences." Kudo S., Fukuda M. Proc. Natl. Acad. Sci. U.S.A. 86:4619-4623(1989) [PubMed: 2734312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-84.
[5]	"Molecular genetics of human erythrocyte MiIII and MiVI glycophorins. Use of a pseudoexon in construction of two delta-alpha-delta hybrid genes resulting in antigenic diversification." Huang C.-H., Blumenfeld O.O. J. Biol. Chem. 266:7248-7255(1991) [PubMed: 2016325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-58. Tissue: Blood.
[6]	"Glycophorins B and C from human erythrocyte membranes. Purification and sequence analysis." Blanchard D., Dahr W., Hummel M., Latron F., Beyreuther K., Cartron J.-P. J. Biol. Chem. 262:5808-5811(1987) [PubMed: 3571235] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-90.
[7]	"Hybrid glycophorins from human erythrocyte membranes. I. Isolation and complete structural analysis of the hybrid sialoglycoprotein from Dantu-positive red cells of the N.E. variety." Dahr W., Beyreuther K., Moulds J., Unger P. Eur. J. Biochem. 166:31-36(1987) [PubMed: 3595615] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-90.
[8]	"Molecular basis for the human erythrocyte glycophorin specifying the Miltenberger class I (MiI) phenotype." Huang C.-H., Spruell P., Moulds J.J., Blumenfeld O.O. Blood 80:257-263(1992) [PubMed: 1611092] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 20-45.

Web resources

BGMUT

Blood group antigen gene mutation database"

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02982 mRNA. Translation: AAA52573.1. X08055 mRNA. Translation: CAB42645.1. M24137 , M24130, M24131, M24135 Genomic DNA. Translation: AAA58626.1. BC069310 mRNA. Translation: AAH69310.1. BC121077 mRNA. Translation: AAI21078.1. BC121078 mRNA. Translation: AAI21079.1. M60708 Genomic DNA. Translation: AAC63048.1.
PIR	B33931.
RefSeq	NP_002091.2.
UniGene	Hs.654368
3D structure databases
HSSP	HSSP built from PDB template 1AFO based on UniProtKB P02724.
ModBase	Search...
PTM databases
GlycoSuiteDB	P06028.
Genome annotation databases
Ensembl	ENSG00000153143. Homo sapiens. [Contig view]
GeneID	2994.
KEGG	hsa:2994.
Organism-specific databases
HGNC	HGNC:4703. GYPB.
MIM	111740. gene+phenotype.
PharmGKB	PA29081.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOVERGEN	P06028.
Gene expression databases
CleanEx	HS_GYPB.
GermOnline	ENSG00000153143. Homo sapiens.
Family and domain databases
InterPro	IPR001195. Glycophorin_A_B. [Graphical view]
PANTHER	PTHR13813. Glycophorin_A_B. 1 hit.
Pfam	PF01102. Glycophorin_A. 1 hit. [Graphical view]
PROSITE	PS00312. GLYCOPHORIN_A. 1 hit. [Graphical view]
ProDom	P06028. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

GLPB_HUMAN

Accession

Primary (citable) accession number: P06028
Secondary accession number(s): Q0VAF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 1, 1990
Last modified:	July 22, 2008
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)