P06028 (GLPB_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 121.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Glycophorin-B Alternative name(s): PAS-3 SS-active sialoglycoprotein Sialoglycoprotein delta CD_antigen=CD235b | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Reference proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 91 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | This protein is a minor sialoglycoprotein in erythrocyte membranes. |
| Subcellular location | |
| Post-translational modification | The N-terminal extracellular domain is heavily glycosylated on serine and threonine residues. Ref.12 |
| Polymorphism | Along with GYPA, GYPB is responsible for the MNS blood group system. The molecular basis of the S/s blood group antigen is a single variation in position 48; Thr-48 corresponds to s=MSN4 and Met-48 to S=MNS3. |
| Sequence similarities | Belongs to the glycophorin-A family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Signal Transmembrane Transmembrane helix |
| Ligand | Sialic acid |
| Molecular function | Blood group antigen |
| PTM | Glycoprotein |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Cellular_component | integral to plasma membrane Traceable author statement PubMed 7622054. Source: ProtInc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | Ref.9 Ref.10 Ref.12 | ||||||
| Chain | 20 – 91 | 72 | Glycophorin-B | PRO_0000012136 | |||||
Regions | |||||||||
| Topological domain | 20 – 59 | 40 | Extracellular Potential | ||||||
| Transmembrane | 60 – 81 | 22 | Helical; Potential | ||||||
| Topological domain | 82 – 91 | 10 | Cytoplasmic Potential | ||||||
Sites | |||||||||
| Site | 33 | 1 | Not glycosylated | ||||||
| Site | 34 | 1 | Not glycosylated | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 36 | 1 | O-linked (GlcNAc...) Ref.12 | ||||||
| Glycosylation | 38 | 1 | O-linked (GlcNAc...) Ref.12 | ||||||
Natural variations | |||||||||
| Natural variant | 22 | 1 | T → S in M(v) antigen. Ref.6 | VAR_047948 | |||||
| Natural variant | 48 | 1 | T → M in S antigen and Mit antigen. Ref.6 Corresponds to variant rs7683365 [ dbSNP | Ensembl ]. | VAR_003192 | |||||
| Natural variant | 54 | 1 | R → H in Mit antigen. Ref.6 | VAR_047949 | |||||
| Natural variant | 58 | 1 | P → R in s(D) antigen. Ref.6 | VAR_047950 | |||||
| Natural variant | 84 | 1 | S → T. Ref.1 Ref.3 Ref.6 Corresponds to variant rs1132783 [ dbSNP | Ensembl ]. | VAR_030785 | |||||
Experimental info | |||||||||
| Sequence conflict | 69 | 1 | C → S AA sequence Ref.9 | ||||||
| Sequence conflict | 69 | 1 | C → S AA sequence Ref.10 | ||||||
Sequences
References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A." Siebert P.D., Fukuda M. Proc. Natl. Acad. Sci. U.S.A. 84:6735-6739(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-84. |
| [2] | "Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta." Tate C.G., Tanner M.J.A. Biochem. J. 254:743-750(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences." Kudo S., Fukuda M. Proc. Natl. Acad. Sci. U.S.A. 86:4619-4623(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-84. |
| [4] | "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. Wilson R.K.Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | "The low-incidence MNS antigens M(v), s(D), and Mit arise from single amino acid substitutions on GPB." Storry J.R., Reid M.E., MacLennan S., Lubenko A., Nortman P. Transfusion 41:269-275(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT M(V) SER-22, VARIANTS MIT MET-48 AND HIS-54, VARIANT S(D) ARG-58, VARIANT THR-84. Tissue: Blood. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [8] | "Molecular genetics of human erythrocyte MiIII and MiVI glycophorins. Use of a pseudoexon in construction of two delta-alpha-delta hybrid genes resulting in antigenic diversification." Huang C.-H., Blumenfeld O.O. J. Biol. Chem. 266:7248-7255(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-58. Tissue: Blood. |
| [9] | "Hybrid glycophorins from human erythrocyte membranes. I. Isolation and complete structural analysis of the hybrid sialoglycoprotein from Dantu-positive red cells of the N.E. variety." Dahr W., Beyreuther K., Moulds J., Unger P. Eur. J. Biochem. 166:31-36(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-90. |
| [10] | "Glycophorins B and C from human erythrocyte membranes. Purification and sequence analysis." Blanchard D., Dahr W., Hummel M., Latron F., Beyreuther K., Cartron J.-P. J. Biol. Chem. 262:5808-5811(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-90. |
| [11] | "Molecular basis for the human erythrocyte glycophorin specifying the Miltenberger class I (MiI) phenotype." Huang C.-H., Spruell P., Moulds J.J., Blumenfeld O.O. Blood 80:257-263(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-45. |
| [12] | "Epitopic structure of Tn glycophorin A for an anti-Tn antibody (MLS 128)." Nakada H., Inoue M., Numata Y., Tanaka N., Funakoshi I., Fukui S., Mellors A., Yamashina I. Proc. Natl. Acad. Sci. U.S.A. 90:2495-2499(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-40, GLYCOSYLATION AT THR-36 AND SER-38. |
| + | Additional computationally mapped references. |
Web resources
| dbRBC/BGMUT Blood group antigen gene mutation database |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | J02982 mRNA. Translation: AAA52573.1. X08055 mRNA. Translation: CAB42645.1. M24137 M24135 Genomic DNA. Translation: AAA58626.1.AY950609 mRNA. Translation: AAX53130.1. AY950610 mRNA. Translation: AAX53131.1. AY950611 mRNA. Translation: AAX53132.1. AC093890 Genomic DNA. No translation available. AC107223 Genomic DNA. No translation available. AC110762 Genomic DNA. No translation available. CH471056 Genomic DNA. Translation: EAX05058.1. BC069310 mRNA. Translation: AAH69310.1. BC121077 mRNA. Translation: AAI21078.1. BC121078 mRNA. Translation: AAI21079.1. M60708 Genomic DNA. Translation: AAC63048.1. |
| IPI | IPI00024995. |
| PIR | B33931. |
| RefSeq | NP_002091.3. NM_002100.4. |
| UniGene | Hs.654368. |
3D structure databases | |
| ProteinModelPortal | P06028. |
| SMR | P06028. Positions 49-91. |
| ModBase | Search... |
PTM databases | |
| GlycoSuiteDB | P06028. |
Polymorphism databases | |
| DMDM | 121405. |
Protocols and materials databases | |
| DNASU | 2994. |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000283126; ENSP00000283126; ENSG00000250361. ENST00000502664; ENSP00000427690; ENSG00000250361. |
| GeneID | 2994. |
| KEGG | hsa:2994. |
| UCSC | uc003ijm.1. human. |
Organism-specific databases | |
| CTD | 2994. |
| GeneCards | GC04M144918. |
| HGNC | HGNC:4703. GYPB. |
| MIM | 111740. gene+phenotype. |
| neXtProt | NX_P06028. |
| PharmGKB | PA29081. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | HOG000089933. |
| HOVERGEN | HBG005850. |
| KO | K06575. |
| OMA | YISSETN. |
Gene expression databases | |
| ArrayExpress | P06028. |
| Bgee | P06028. |
| CleanEx | HS_GYPB. |
| Genevestigator | P06028. |
| GermOnline | ENSG00000153143. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001195. Glycophorin. IPR018938. Glycophorin_CS. [Graphical view] |
| PANTHER | PTHR13813. PTHR13813. 1 hit. |
| Pfam | PF01102. Glycophorin_A. 1 hit. [Graphical view] |
| PROSITE | PS00312. GLYCOPHORIN_A. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| GenomeRNAi | 2994. |
| NextBio | 11866. |
| SOURCE | Search... |
Entry information
| Entry name | GLPB_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P06028 Secondary accession number(s): E2QBW7 Q9UCH7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Blood group antigen proteins Nomenclature of blood group antigens and list of entries |
| Human cell differentiation molecules CD nomenclature of surface proteins of human leucocytes and list of entries |
| Human chromosome 4 Human chromosome 4: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with
