Reviewed, UniProtKB/Swiss-Prot P06012 (ORF2A_CMVQ)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase 2A
      Short name=protein 2A
    EC=2.7.7.48

Gene names

Name:	RNA2
ORF Names:	ORF2a

Organism

Cucumber mosaic virus (strain Q) (CMV)

Taxonomic identifier

12310 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Cucumovirus

Virus host

Cucumis sativus (Cucumber) [TaxID: 3659]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Nicotiana tabacum (Common tobacco) [TaxID: 4097]

Protein attributes

Sequence length	839 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-directed RNA polymerase.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Miscellaneous	The virus has a tripartite genome (RNA1, RNA2 and RNA3) and an encapsidated subgenomic RNA (RNA4) from which the coat protein is expressed. Virions are icosahedral, not enveloped, with 32 capsomers per nucleocapsid.
Sequence similarities	Belongs to the cucumoviruses/bromoviruses 2A family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 839	839	RNA-directed RNA polymerase 2A		PRO_0000083280
Regions
	Domain	508 – 621	114	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P06012-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 42C8E0B9D56A8BD9	FASTA	839	94,440
	10 20 30 40 50 60 MISPPPTFSF ANLLNGSYGV DTPEEVERVR REQREDAEAA LRNYKPLPAV DVSESVPRDE 70 80 90 100 110 120 PIVSQTVTAA PVTSVDDAFV SFGAEDYLEM SPSELLSAFE LMVKPLRVGE VLCSSFDRSL 130 140 150 160 170 180 FISSVAMART LLLAPLTSTR TLKRFEDLVA AIYLKTDFFL EDDGPQTDVS QSDVPGYIFE 190 200 210 220 230 240 PGQHSSGFEP PPICAKCDLI LYQCPCFDFN ALRESCAEKT FSHDYVIEGL DGVIDNATLL 250 260 270 280 290 300 SNLGPFLLPV HCSYSKTEDP DFVVDPSLAR PTDRVDVHVV QAVCDTTLPT HGNYDDSFHQ 310 320 330 340 350 360 VFVDSADYST DMDHVRLRQS DLVAKIPDGG HMLPVLNTGS GHQRVGTTKE VLTAIKKRNA 370 380 390 400 410 420 DVPELGDSVN LSRLSKAVAE RFRLSYMNVD ALAKSNFVNV VSNFHAYMQK WPSSGLSYDD 430 440 450 460 470 480 LPDLHAENLQ FYDHMIKSDV KPVVTDTLNV DRPVPATITF HKKTITSQFS PLFISLFERF 490 500 510 520 530 540 QRCLRERVVL PVGKISSLEM TGFSVLNKHC LEIDLSKFDK SQGEFHLMIQ EHILNDLGCP 550 560 570 580 590 600 APITKWWCDF HRFSYIKDKR AGVGMPISFQ RRTGDAFTYF GNTIVTMAEF AWCYDTDQFD 610 620 630 640 650 660 RLLFSGDDSL AFSKLPPVGD PSKFTTLFNM EAKVMEPAVP YICSKFYSLM SLVTRFQSPT 670 680 690 700 710 720 IREIQRLGTK KIPYSDNNDF LFAHFMSFVD RLKFMDRMSQ SCIDQLSIFF ELKYKKSGNE 730 740 750 760 770 780 AALVLGAFKK YTANFNAYKE LYYSDRQQCD LVNTFCISEF RVIRRTTVKK KKNGCVDSSG 790 800 810 820 830 VDRRPPLSQF AGGETSKTKV SRQKPASEGL QKSQRESAIY SETFPDVTIP RSRSRGLVS

References

[1]

"Nucleotide sequence of cucumber mosaic virus RNA 2 reveals a translation product significantly homologous to corresponding proteins of other viruses." Rezaian M.A., Williams R.H.V., Gordon K.H.J., Gould A.R., Symons R.H. Eur. J. Biochem. 143:277-284(1984) [PubMed: 6547906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X00985 Genomic RNA. Translation: CAA25494.1.
PIR	S07226.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001788. RNA-dep_RNA_pol_vir-typ. IPR007094. RNA_pol_PSvir. [Graphical view]
Pfam	PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ORF2A_CMVQ

Accession

Primary (citable) accession number: P06012

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)