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Protein

Reaction center protein M chain

Gene

pufM

Organism
Blastochloris viridis (Rhodopseudomonas viridis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi181 – 1811Magnesium (bacteriochlorophyll b axial ligand)
Metal bindingi201 – 2011Magnesium (bacteriochlorophyll b axial ligand)
Metal bindingi218 – 2181Iron
Metal bindingi233 – 2331Iron
Binding sitei251 – 2511Quinone A
Metal bindingi265 – 2651Iron

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Reaction center protein M chain
Alternative name(s):
Photosynthetic reaction center M subunit
Gene namesi
Name:pufM
OrganismiBlastochloris viridis (Rhodopseudomonas viridis)
Taxonomic identifieri1079 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesHyphomicrobiaceaeBlastochloris

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 5150Cytoplasmic1 PublicationAdd
BLAST
Transmembranei52 – 7625HelicalAdd
BLAST
Topological domaini77 – 11034Periplasmic1 PublicationAdd
BLAST
Transmembranei111 – 13727HelicalAdd
BLAST
Topological domaini138 – 1425Cytoplasmic1 Publication
Transmembranei143 – 16624HelicalAdd
BLAST
Topological domaini167 – 19731Periplasmic1 PublicationAdd
BLAST
Transmembranei198 – 22326HelicalAdd
BLAST
Topological domaini224 – 25936Cytoplasmic1 PublicationAdd
BLAST
Transmembranei260 – 28425HelicalAdd
BLAST
Topological domaini285 – 32440Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Reaction center

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 324323Reaction center protein M chainPRO_0000090420Add
BLAST

Interactioni

Subunit structurei

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

Structurei

Secondary structure

1
324
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Beta strandi10 – 145Combined sources
Helixi26 – 283Combined sources
Beta strandi29 – 313Combined sources
Helixi39 – 413Combined sources
Beta strandi45 – 473Combined sources
Helixi54 – 7724Combined sources
Turni78 – 803Combined sources
Helixi82 – 887Combined sources
Helixi89 – 913Combined sources
Helixi108 – 1103Combined sources
Helixi112 – 13827Combined sources
Helixi144 – 16017Combined sources
Helixi162 – 1676Combined sources
Helixi170 – 1723Combined sources
Helixi178 – 19114Combined sources
Helixi195 – 1973Combined sources
Helixi199 – 22426Combined sources
Helixi226 – 2283Combined sources
Turni229 – 2313Combined sources
Helixi233 – 2386Combined sources
Helixi242 – 25514Combined sources
Helixi263 – 28523Combined sources
Turni287 – 2893Combined sources
Helixi293 – 2997Combined sources
Helixi316 – 3183Combined sources
Beta strandi319 – 3213Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DXRX-ray2.00M2-324[»]
1PRCX-ray2.30M2-324[»]
1R2CX-ray2.86M2-324[»]
1VRNX-ray2.20M2-324[»]
2I5NX-ray1.96M2-324[»]
2JBLX-ray2.40M2-324[»]
2PRCX-ray2.45M2-324[»]
2WJMX-ray1.95M1-324[»]
2WJNX-ray1.86M1-324[»]
2X5UX-ray3.00M1-324[»]
2X5VX-ray3.00M1-324[»]
3D38X-ray3.21M2-324[»]
3G7FX-ray2.50M2-324[»]
3PRCX-ray2.40M2-324[»]
3T6DX-ray1.95M2-324[»]
3T6EX-ray1.92M2-324[»]
4AC5X-ray8.20M1-324[»]
4CASX-ray3.50C1-324[»]
5PRCX-ray2.35M2-324[»]
6PRCX-ray2.30M2-324[»]
7PRCX-ray2.65M2-324[»]
ProteinModelPortaliP06010.
SMRiP06010. Positions 2-324.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06010.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd09291. Photo-RC_M. 1 hit.
Gene3Di1.20.85.10. 2 hits.
InterProiIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01115. pufM. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADYQTIYTQ IQARGPHITV SGEWGDNDRV GKPFYSYWLG KIGDAQIGPI
60 70 80 90 100
YLGASGIAAF AFGSTAILII LFNMAAEVHF DPLQFFRQFF WLGLYPPKAQ
110 120 130 140 150
YGMGIPPLHD GGWWLMAGLF MTLSLGSWWI RVYSRARALG LGTHIAWNFA
160 170 180 190 200
AAIFFVLCIG CIHPTLVGSW SEGVPFGIWP HIDWLTAFSI RYGNFYYCPW
210 220 230 240 250
HGFSIGFAYG CGLLFAAHGA TILAVARFGG DREIEQITDR GTAVERAALF
260 270 280 290 300
WRWTIGFNAT IESVHRWGWF FSLMVMVSAS VGILLTGTFV DNWYLWCVKH
310 320
GAAPDYPAYL PATPDPASLP GAPK
Length:324
Mass (Da):36,036
Last modified:January 23, 2007 - v3
Checksum:i28C33C28A0CCA945
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03915 Genomic DNA. Translation: CAA27551.1.
PIRiB25102.
RefSeqiWP_055036367.1. NZ_LN907867.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03915 Genomic DNA. Translation: CAA27551.1.
PIRiB25102.
RefSeqiWP_055036367.1. NZ_LN907867.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DXRX-ray2.00M2-324[»]
1PRCX-ray2.30M2-324[»]
1R2CX-ray2.86M2-324[»]
1VRNX-ray2.20M2-324[»]
2I5NX-ray1.96M2-324[»]
2JBLX-ray2.40M2-324[»]
2PRCX-ray2.45M2-324[»]
2WJMX-ray1.95M1-324[»]
2WJNX-ray1.86M1-324[»]
2X5UX-ray3.00M1-324[»]
2X5VX-ray3.00M1-324[»]
3D38X-ray3.21M2-324[»]
3G7FX-ray2.50M2-324[»]
3PRCX-ray2.40M2-324[»]
3T6DX-ray1.95M2-324[»]
3T6EX-ray1.92M2-324[»]
4AC5X-ray8.20M1-324[»]
4CASX-ray3.50C1-324[»]
5PRCX-ray2.35M2-324[»]
6PRCX-ray2.30M2-324[»]
7PRCX-ray2.65M2-324[»]
ProteinModelPortaliP06010.
SMRiP06010. Positions 2-324.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP06010.

Family and domain databases

CDDicd09291. Photo-RC_M. 1 hit.
Gene3Di1.20.85.10. 2 hits.
InterProiIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01115. pufM. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRCEM_BLAVI
AccessioniPrimary (citable) accession number: P06010
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.