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Protein

Reaction center protein M chain

Gene

pufM

Organism
Blastochloris viridis (Rhodopseudomonas viridis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi181Magnesium (bacteriochlorophyll b axial ligand)1
Metal bindingi201Magnesium (bacteriochlorophyll b axial ligand)1
Metal bindingi218Iron1
Metal bindingi233Iron1
Binding sitei251Quinone A1
Metal bindingi265Iron1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Reaction center protein M chain
Alternative name(s):
Photosynthetic reaction center M subunit
Gene namesi
Name:pufM
OrganismiBlastochloris viridis (Rhodopseudomonas viridis)
Taxonomic identifieri1079 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesHyphomicrobiaceaeBlastochloris

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 51Cytoplasmic1 PublicationAdd BLAST50
Transmembranei52 – 76HelicalAdd BLAST25
Topological domaini77 – 110Periplasmic1 PublicationAdd BLAST34
Transmembranei111 – 137HelicalAdd BLAST27
Topological domaini138 – 142Cytoplasmic1 Publication5
Transmembranei143 – 166HelicalAdd BLAST24
Topological domaini167 – 197Periplasmic1 PublicationAdd BLAST31
Transmembranei198 – 223HelicalAdd BLAST26
Topological domaini224 – 259Cytoplasmic1 PublicationAdd BLAST36
Transmembranei260 – 284HelicalAdd BLAST25
Topological domaini285 – 324Periplasmic1 PublicationAdd BLAST40

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Reaction center

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000904202 – 324Reaction center protein M chainAdd BLAST323

Interactioni

Subunit structurei

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).

Structurei

Secondary structure

1324
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi10 – 14Combined sources5
Helixi26 – 28Combined sources3
Beta strandi29 – 31Combined sources3
Helixi39 – 41Combined sources3
Beta strandi45 – 47Combined sources3
Helixi54 – 77Combined sources24
Turni78 – 80Combined sources3
Helixi82 – 88Combined sources7
Helixi89 – 91Combined sources3
Helixi108 – 110Combined sources3
Helixi112 – 138Combined sources27
Helixi144 – 160Combined sources17
Helixi162 – 167Combined sources6
Helixi170 – 172Combined sources3
Helixi178 – 191Combined sources14
Helixi195 – 197Combined sources3
Helixi199 – 224Combined sources26
Helixi226 – 228Combined sources3
Turni229 – 231Combined sources3
Helixi233 – 238Combined sources6
Helixi242 – 255Combined sources14
Helixi263 – 285Combined sources23
Turni287 – 289Combined sources3
Helixi293 – 299Combined sources7
Helixi316 – 318Combined sources3
Beta strandi319 – 321Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DXRX-ray2.00M2-324[»]
1PRCX-ray2.30M2-324[»]
1R2CX-ray2.86M2-324[»]
1VRNX-ray2.20M2-324[»]
2I5NX-ray1.96M2-324[»]
2JBLX-ray2.40M2-324[»]
2PRCX-ray2.45M2-324[»]
2WJMX-ray1.95M1-324[»]
2WJNX-ray1.86M1-324[»]
2X5UX-ray3.00M1-324[»]
2X5VX-ray3.00M1-324[»]
3D38X-ray3.21M2-324[»]
3G7FX-ray2.50M2-324[»]
3PRCX-ray2.40M2-324[»]
3T6DX-ray1.95M2-324[»]
3T6EX-ray1.92M2-324[»]
4AC5X-ray8.20M1-324[»]
4CASX-ray3.50C1-324[»]
5PRCX-ray2.35M2-324[»]
6PRCX-ray2.30M2-324[»]
7PRCX-ray2.65M2-324[»]
ProteinModelPortaliP06010.
SMRiP06010.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06010.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd09291. Photo-RC_M. 1 hit.
Gene3Di1.20.85.10. 2 hits.
InterProiIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01115. pufM. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADYQTIYTQ IQARGPHITV SGEWGDNDRV GKPFYSYWLG KIGDAQIGPI
60 70 80 90 100
YLGASGIAAF AFGSTAILII LFNMAAEVHF DPLQFFRQFF WLGLYPPKAQ
110 120 130 140 150
YGMGIPPLHD GGWWLMAGLF MTLSLGSWWI RVYSRARALG LGTHIAWNFA
160 170 180 190 200
AAIFFVLCIG CIHPTLVGSW SEGVPFGIWP HIDWLTAFSI RYGNFYYCPW
210 220 230 240 250
HGFSIGFAYG CGLLFAAHGA TILAVARFGG DREIEQITDR GTAVERAALF
260 270 280 290 300
WRWTIGFNAT IESVHRWGWF FSLMVMVSAS VGILLTGTFV DNWYLWCVKH
310 320
GAAPDYPAYL PATPDPASLP GAPK
Length:324
Mass (Da):36,036
Last modified:January 23, 2007 - v3
Checksum:i28C33C28A0CCA945
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03915 Genomic DNA. Translation: CAA27551.1.
PIRiB25102.
RefSeqiWP_055036367.1. NZ_LN907867.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03915 Genomic DNA. Translation: CAA27551.1.
PIRiB25102.
RefSeqiWP_055036367.1. NZ_LN907867.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DXRX-ray2.00M2-324[»]
1PRCX-ray2.30M2-324[»]
1R2CX-ray2.86M2-324[»]
1VRNX-ray2.20M2-324[»]
2I5NX-ray1.96M2-324[»]
2JBLX-ray2.40M2-324[»]
2PRCX-ray2.45M2-324[»]
2WJMX-ray1.95M1-324[»]
2WJNX-ray1.86M1-324[»]
2X5UX-ray3.00M1-324[»]
2X5VX-ray3.00M1-324[»]
3D38X-ray3.21M2-324[»]
3G7FX-ray2.50M2-324[»]
3PRCX-ray2.40M2-324[»]
3T6DX-ray1.95M2-324[»]
3T6EX-ray1.92M2-324[»]
4AC5X-ray8.20M1-324[»]
4CASX-ray3.50C1-324[»]
5PRCX-ray2.35M2-324[»]
6PRCX-ray2.30M2-324[»]
7PRCX-ray2.65M2-324[»]
ProteinModelPortaliP06010.
SMRiP06010.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP06010.

Family and domain databases

CDDicd09291. Photo-RC_M. 1 hit.
Gene3Di1.20.85.10. 2 hits.
InterProiIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01115. pufM. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRCEM_BLAVI
AccessioniPrimary (citable) accession number: P06010
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.