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Protein

cAMP-dependent protein kinase type 3

Gene

TPK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by cAMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171ATPPROSITE-ProRule annotation
Active sitei211 – 2111Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi94 – 1029ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cAMP-dependent protein kinase activity Source: SGD

GO - Biological processi

  • mitochondrion organization Source: SGD
  • protein kinase A signaling Source: SGD
  • protein phosphorylation Source: SGD
  • Ras protein signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, cAMP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31934-MONOMER.
BRENDAi2.7.11.11. 984.
ReactomeiR-SCE-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-SCE-163615. PKA activation.
R-SCE-164378. PKA activation in glucagon signalling.
R-SCE-180024. DARPP-32 events.
R-SCE-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-SCE-5610787. Hedgehog 'off' state.
R-SCE-5687128. MAPK6/MAPK4 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type 3 (EC:2.7.11.11)
Short name:
PKA 3
Gene namesi
Name:TPK3
Synonyms:PKA3
Ordered Locus Names:YKL166C
ORF Names:YKL630
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL166C.
SGDiS000001649. TPK3.

Subcellular locationi

GO - Cellular componenti

  • cAMP-dependent protein kinase complex Source: SGD
  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398cAMP-dependent protein kinase type 3PRO_0000086071Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151PhosphoserineCombined sources
Modified residuei55 – 551PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP05986.
PeptideAtlasiP05986.

PTM databases

iPTMnetiP05986.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BCY1P072784EBI-9470,EBI-9475

Protein-protein interaction databases

BioGridi33971. 217 interactions.
DIPiDIP-550N.
IntActiP05986. 5 interactions.
MINTiMINT-398395.

Structurei

3D structure databases

ProteinModelPortaliP05986.
SMRiP05986. Positions 56-398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini88 – 342255Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini343 – 39856AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00820000127176.
HOGENOMiHOG000233033.
InParanoidiP05986.
KOiK04345.
OMAiLFMIMDY.
OrthoDBiEOG7R2BT5.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI
60 70 80 90 100
NGRNSGKLKE EASAGICLVK KPMLQYRDTS GKYSLSDFQI LRTLGTGSFG
110 120 130 140 150
RVHLIRSNHN GRFYALKTLK KHTIVKLKQV EHTNDERRML SIVSHPFIIR
160 170 180 190 200
MWGTFQDSQQ VFMVMDYIEG GELFSLLRKS QRFPNPVAKF YAAEVCLALE
210 220 230 240 250
YLHSKDIIYR DLKPENILLD KNGHIKITDF GFAKYVPDVT YTLCGTPDYI
260 270 280 290 300
APEVVSTKPY NKSVDWWSFG VLIYEMLAGY TPFYNSNTMK TYENILNAEL
310 320 330 340 350
KFPPFFHPDA QDLLKKLITR DLSERLGNLQ NGSEDVKNHP WFNEVIWEKL
360 370 380 390
LARYIETPYE PPIQQGQGDT SQFDRYPEEE FNYGIQGEDP YMDLMKEF
Length:398
Mass (Da):45,977
Last modified:June 1, 1994 - v2
Checksum:i4ADDE8E63032B313
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41D → E in AAA35166 (PubMed:3036373).Curated
Sequence conflicti208 – 2081I → T in AAA35166 (PubMed:3036373).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17074 Genomic DNA. Translation: AAA35166.1.
Z26878 Genomic DNA. Translation: CAA81521.1.
Z28166 Genomic DNA. Translation: CAA82008.1.
M81697 Genomic DNA. Translation: AAA34791.1.
BK006944 Genomic DNA. Translation: DAA08999.1.
PIRiS37996. OKBYC3.
RefSeqiNP_012755.1. NM_001179732.1.

Genome annotation databases

EnsemblFungiiYKL166C; YKL166C; YKL166C.
GeneIDi853688.
KEGGisce:YKL166C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17074 Genomic DNA. Translation: AAA35166.1.
Z26878 Genomic DNA. Translation: CAA81521.1.
Z28166 Genomic DNA. Translation: CAA82008.1.
M81697 Genomic DNA. Translation: AAA34791.1.
BK006944 Genomic DNA. Translation: DAA08999.1.
PIRiS37996. OKBYC3.
RefSeqiNP_012755.1. NM_001179732.1.

3D structure databases

ProteinModelPortaliP05986.
SMRiP05986. Positions 56-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33971. 217 interactions.
DIPiDIP-550N.
IntActiP05986. 5 interactions.
MINTiMINT-398395.

PTM databases

iPTMnetiP05986.

Proteomic databases

MaxQBiP05986.
PeptideAtlasiP05986.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL166C; YKL166C; YKL166C.
GeneIDi853688.
KEGGisce:YKL166C.

Organism-specific databases

EuPathDBiFungiDB:YKL166C.
SGDiS000001649. TPK3.

Phylogenomic databases

GeneTreeiENSGT00820000127176.
HOGENOMiHOG000233033.
InParanoidiP05986.
KOiK04345.
OMAiLFMIMDY.
OrthoDBiEOG7R2BT5.

Enzyme and pathway databases

BioCyciYEAST:G3O-31934-MONOMER.
BRENDAi2.7.11.11. 984.
ReactomeiR-SCE-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-SCE-163615. PKA activation.
R-SCE-164378. PKA activation in glucagon signalling.
R-SCE-180024. DARPP-32 events.
R-SCE-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-SCE-5610787. Hedgehog 'off' state.
R-SCE-5687128. MAPK6/MAPK4 signaling.

Miscellaneous databases

NextBioi974661.
PROiP05986.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase."
    Toda T., Cameron S., Sass P., Zoller M., Wigler M.
    Cell 50:277-287(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Structure and function of MRP20 and MRP49, the nuclear genes for two proteins of the 54 S subunit of the yeast mitochondrial ribosome."
    Fearon K., Mason T.L.
    J. Biol. Chem. 267:5162-5170(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 344-398.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKAPC_YEAST
AccessioniPrimary (citable) accession number: P05986
Secondary accession number(s): D6VX33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 166 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.