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Protein

cAMP-dependent protein kinase type 3

Gene

TPK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by cAMP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117ATPPROSITE-ProRule annotation1
Active sitei211Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi94 – 102ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cAMP-dependent protein kinase activity Source: SGD

GO - Biological processi

  • mitochondrion organization Source: SGD
  • negative regulation of cytoplasmic mRNA processing body assembly Source: SGD
  • negative regulation of cytoplasmic translation Source: SGD
  • protein kinase A signaling Source: SGD
  • protein phosphorylation Source: SGD
  • Ras protein signal transduction Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, cAMP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31934-MONOMER
BRENDAi2.7.11.11 984

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type 3 (EC:2.7.11.11)
Short name:
PKA 3
Gene namesi
Name:TPK3
Synonyms:PKA3
Ordered Locus Names:YKL166C
ORF Names:YKL630
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL166C
SGDiS000001649 TPK3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860711 – 398cAMP-dependent protein kinase type 3Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1
Modified residuei55PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP05986
PaxDbiP05986
PRIDEiP05986

PTM databases

iPTMnetiP05986

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BCY1P072784EBI-9470,EBI-9475

Protein-protein interaction databases

BioGridi33971317 interactors.
DIPiDIP-550N
IntActiP05986 28 interactors.
MINTiP05986
STRINGi4932.YKL166C

Structurei

3D structure databases

ProteinModelPortaliP05986
SMRiP05986
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 342Protein kinasePROSITE-ProRule annotationAdd BLAST255
Domaini343 – 398AGC-kinase C-terminalAdd BLAST56

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00810000125385
HOGENOMiHOG000233033
InParanoidiP05986
KOiK04345
OMAiKICEADY
OrthoDBiEOG092C0HH3

Family and domain databases

InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P05986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI
60 70 80 90 100
NGRNSGKLKE EASAGICLVK KPMLQYRDTS GKYSLSDFQI LRTLGTGSFG
110 120 130 140 150
RVHLIRSNHN GRFYALKTLK KHTIVKLKQV EHTNDERRML SIVSHPFIIR
160 170 180 190 200
MWGTFQDSQQ VFMVMDYIEG GELFSLLRKS QRFPNPVAKF YAAEVCLALE
210 220 230 240 250
YLHSKDIIYR DLKPENILLD KNGHIKITDF GFAKYVPDVT YTLCGTPDYI
260 270 280 290 300
APEVVSTKPY NKSVDWWSFG VLIYEMLAGY TPFYNSNTMK TYENILNAEL
310 320 330 340 350
KFPPFFHPDA QDLLKKLITR DLSERLGNLQ NGSEDVKNHP WFNEVIWEKL
360 370 380 390
LARYIETPYE PPIQQGQGDT SQFDRYPEEE FNYGIQGEDP YMDLMKEF
Length:398
Mass (Da):45,977
Last modified:June 1, 1994 - v2
Checksum:i4ADDE8E63032B313
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4D → E in AAA35166 (PubMed:3036373).Curated1
Sequence conflicti208I → T in AAA35166 (PubMed:3036373).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17074 Genomic DNA Translation: AAA35166.1
Z26878 Genomic DNA Translation: CAA81521.1
Z28166 Genomic DNA Translation: CAA82008.1
M81697 Genomic DNA Translation: AAA34791.1
BK006944 Genomic DNA Translation: DAA08999.1
PIRiS37996 OKBYC3
RefSeqiNP_012755.1, NM_001179732.1

Genome annotation databases

EnsemblFungiiYKL166C; YKL166C; YKL166C
GeneIDi853688
KEGGisce:YKL166C

Similar proteinsi

Entry informationi

Entry nameiKAPC_YEAST
AccessioniPrimary (citable) accession number: P05986
Secondary accession number(s): D6VX33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 184 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome