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Protein

cAMP-dependent protein kinase type 3

Gene

TPK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by cAMP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117ATPPROSITE-ProRule annotation1
Active sitei211Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi94 – 102ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cAMP-dependent protein kinase activity Source: SGD

GO - Biological processi

  • mitochondrion organization Source: SGD
  • protein kinase A signaling Source: SGD
  • protein phosphorylation Source: SGD
  • Ras protein signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, cAMP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31934-MONOMER.
BRENDAi2.7.11.11. 984.
ReactomeiR-SCE-166208. mTORC1-mediated signalling.
R-SCE-198693. AKT phosphorylates targets in the nucleus.
R-SCE-198753. ERK/MAPK targets.
R-SCE-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-SCE-375165. NCAM signaling for neurite out-growth.
R-SCE-444257. RSK activation.
R-SCE-881907. Gastrin-CREB signalling pathway via PKC and MAPK.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type 3 (EC:2.7.11.11)
Short name:
PKA 3
Gene namesi
Name:TPK3
Synonyms:PKA3
Ordered Locus Names:YKL166C
ORF Names:YKL630
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL166C.
SGDiS000001649. TPK3.

Subcellular locationi

GO - Cellular componenti

  • cAMP-dependent protein kinase complex Source: SGD
  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860711 – 398cAMP-dependent protein kinase type 3Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1
Modified residuei55PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP05986.
PRIDEiP05986.

PTM databases

iPTMnetiP05986.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BCY1P072784EBI-9470,EBI-9475

Protein-protein interaction databases

BioGridi33971. 218 interactors.
DIPiDIP-550N.
IntActiP05986. 5 interactors.
MINTiMINT-398395.

Structurei

3D structure databases

ProteinModelPortaliP05986.
SMRiP05986.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 342Protein kinasePROSITE-ProRule annotationAdd BLAST255
Domaini343 – 398AGC-kinase C-terminalAdd BLAST56

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00860000135182.
HOGENOMiHOG000233033.
InParanoidiP05986.
KOiK04345.
OMAiNHNGRFY.
OrthoDBiEOG092C0HH3.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI
60 70 80 90 100
NGRNSGKLKE EASAGICLVK KPMLQYRDTS GKYSLSDFQI LRTLGTGSFG
110 120 130 140 150
RVHLIRSNHN GRFYALKTLK KHTIVKLKQV EHTNDERRML SIVSHPFIIR
160 170 180 190 200
MWGTFQDSQQ VFMVMDYIEG GELFSLLRKS QRFPNPVAKF YAAEVCLALE
210 220 230 240 250
YLHSKDIIYR DLKPENILLD KNGHIKITDF GFAKYVPDVT YTLCGTPDYI
260 270 280 290 300
APEVVSTKPY NKSVDWWSFG VLIYEMLAGY TPFYNSNTMK TYENILNAEL
310 320 330 340 350
KFPPFFHPDA QDLLKKLITR DLSERLGNLQ NGSEDVKNHP WFNEVIWEKL
360 370 380 390
LARYIETPYE PPIQQGQGDT SQFDRYPEEE FNYGIQGEDP YMDLMKEF
Length:398
Mass (Da):45,977
Last modified:June 1, 1994 - v2
Checksum:i4ADDE8E63032B313
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4D → E in AAA35166 (PubMed:3036373).Curated1
Sequence conflicti208I → T in AAA35166 (PubMed:3036373).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17074 Genomic DNA. Translation: AAA35166.1.
Z26878 Genomic DNA. Translation: CAA81521.1.
Z28166 Genomic DNA. Translation: CAA82008.1.
M81697 Genomic DNA. Translation: AAA34791.1.
BK006944 Genomic DNA. Translation: DAA08999.1.
PIRiS37996. OKBYC3.
RefSeqiNP_012755.1. NM_001179732.1.

Genome annotation databases

EnsemblFungiiYKL166C; YKL166C; YKL166C.
GeneIDi853688.
KEGGisce:YKL166C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17074 Genomic DNA. Translation: AAA35166.1.
Z26878 Genomic DNA. Translation: CAA81521.1.
Z28166 Genomic DNA. Translation: CAA82008.1.
M81697 Genomic DNA. Translation: AAA34791.1.
BK006944 Genomic DNA. Translation: DAA08999.1.
PIRiS37996. OKBYC3.
RefSeqiNP_012755.1. NM_001179732.1.

3D structure databases

ProteinModelPortaliP05986.
SMRiP05986.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33971. 218 interactors.
DIPiDIP-550N.
IntActiP05986. 5 interactors.
MINTiMINT-398395.

PTM databases

iPTMnetiP05986.

Proteomic databases

MaxQBiP05986.
PRIDEiP05986.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL166C; YKL166C; YKL166C.
GeneIDi853688.
KEGGisce:YKL166C.

Organism-specific databases

EuPathDBiFungiDB:YKL166C.
SGDiS000001649. TPK3.

Phylogenomic databases

GeneTreeiENSGT00860000135182.
HOGENOMiHOG000233033.
InParanoidiP05986.
KOiK04345.
OMAiNHNGRFY.
OrthoDBiEOG092C0HH3.

Enzyme and pathway databases

BioCyciYEAST:G3O-31934-MONOMER.
BRENDAi2.7.11.11. 984.
ReactomeiR-SCE-166208. mTORC1-mediated signalling.
R-SCE-198693. AKT phosphorylates targets in the nucleus.
R-SCE-198753. ERK/MAPK targets.
R-SCE-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-SCE-375165. NCAM signaling for neurite out-growth.
R-SCE-444257. RSK activation.
R-SCE-881907. Gastrin-CREB signalling pathway via PKC and MAPK.

Miscellaneous databases

PROiP05986.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAPC_YEAST
AccessioniPrimary (citable) accession number: P05986
Secondary accession number(s): D6VX33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 172 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.