P05976 (MYL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin light chain 1/3, skeletal muscle isoform
Short name=MLC1/MLC3
Short name=MLC1F/MLC3F

Alternative name(s):
Myosin light chain alkali 1/2
Short name=Myosin light chain A1/A2

Gene names

Name:

MYL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory light chain of myosin. Does not bind calcium.
Subunit structure	Myosin is a hexamer of 2 heavy chains and 4 light chains.
Post-translational modification	Isoform MLC3 is acetylated at position 2 By similarity.
Sequence similarities	Contains 3 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Motor protein Muscle protein Myosin
PTM	Acetylation Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle contraction Inferred from electronic annotation. Source: Compara muscle filament sliding Non-traceable author statement PubMed 3904738. Source: BHF-UCL muscle organ development Non-traceable author statement. Source: UniProtKB
Cellular_component	cytosol Traceable author statement. Source: Reactome muscle myosin complex Non-traceable author statement PubMed 3904738. Source: BHF-UCL sarcomere Non-traceable author statement PubMed 3904738. Source: BHF-UCL
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro structural constituent of muscle Non-traceable author statement PubMed 3904738. Source: BHF-UCL
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform MLC1 (identifier: P05976-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform MLC3 (identifier: P05976-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: MAPKKDVKKPVAAAAAAPAPAPAPAPAPAPAKPKEEKIDLSAIKIEFSKEQQD → MSFSADQIA

Note: Initiator Met-1 is removed. Contains a N-acetylalanine at position 2 (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 194	193	Myosin light chain 1/3, skeletal muscle isoform		PRO_0000198681
Regions
	Domain	50 – 85	36	EF-hand 1
	Domain	127 – 162	36	EF-hand 2
	Domain	162 – 194	33	EF-hand 3
Amino acid modifications
	Modified residue	2	1	N,N,N-trimethylalanine By similarity
Natural variations
	Alternative sequence	1 – 53	53	MAPKK…KEQQD → MSFSADQIA in isoform MLC3.		VSP_038686
Experimental info
	Sequence conflict	45	1	I → M in CAB42646. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform MLC1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: DA1F5C5E4C8A2124	FASTA	194	21,145
	10 20 30 40 50 60 MAPKKDVKKP VAAAAAAPAP APAPAPAPAP AKPKEEKIDL SAIKIEFSKE QQDEFKEAFL 70 80 90 100 110 120 LFDRTGDSKI TLSQVGDVLR ALGTNPTNAE VRKVLGNPSN EELNAKKIEF EQFLPMMQAI 130 140 150 160 170 180 SNNKDQATYE DFVEGLRVFD KEGNGTVMGA ELRHVLATLG EKMKEEEVEA LMAGQEDSNG 190 CINYEAFVKH IMSI
	Isoform MLC3 [UniParc] [UniParc]. Checksum: 39D6FC3AD153483A	FASTA	150	16,684
	10 20 30 40 50 60 MSFSADQIAE FKEAFLLFDR TGDSKITLSQ VGDVLRALGT NPTNAEVRKV LGNPSNEELN 70 80 90 100 110 120 AKKIEFEQFL PMMQAISNNK DQATYEDFVE GLRVFDKEGN GTVMGAELRH VLATLGEKMK 130 140 150 EEEVEALMAG QEDSNGCINY EAFVKHIMSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete nucleotide sequences of cDNA clones coding for human myosin light chains 1 and 3." Seidel U., Bober E., Winter B., Lenz S., Lohse P., Arnold H.H. Nucleic Acids Res. 15:4989-4989(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MLC1 AND MLC3). Tissue: Fetal muscle.
[2]	"Alkali myosin light chains in man are encoded by a multigene family that includes the adult skeletal muscle, the embryonic or atrial, and nonsarcomeric isoforms." Seidel U., Bober E., Winter B., Lenz S., Lohse P., Goedde H., Grzeschik K., Arnold H.H. Gene 66:135-146(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MLC1 AND MLC3).
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM MLC1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS MLC1 AND MLC3). Tissue: Skeletal muscle.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM MLC3). Tissue: Liver.
[7]	"Identification of the functional promoter regions in the human gene encoding the myosin alkali light chains MLC1 and MLC3 of fast skeletal muscle." Seidel U., Arnold H.H. J. Biol. Chem. 264:16109-16117(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05450 mRNA. Translation: CAB42646.1. X05451 mRNA. Translation: CAA29020.1. M20642 mRNA. Translation: AAA59854.1. M20643 mRNA. Translation: AAA59855.1. CR456869 mRNA. Translation: CAG33150.1. AK311942 mRNA. Translation: BAG34883.1. AK311892 mRNA. Translation: BAG34833.1. CH471063 Genomic DNA. Translation: EAW70483.1. CH471063 Genomic DNA. Translation: EAW70484.1. BC005318 mRNA. Translation: AAH05318.1. J05026 Genomic DNA. Translation: AAA66960.1.
IPI	IPI00216070. IPI00220332.
PIR	MOHUA1. JS0431. MOHUA2. S07939.
RefSeq	NP_524144.1. NM_079420.2. NP_524146.1. NM_079422.2.
UniGene	Hs.187338.
3D structure databases
ProteinModelPortal	P05976.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05976. 1 interaction.
STRING	9606.ENSP00000307280.
PTM databases
PhosphoSite	P05976.
Polymorphism databases
DMDM	127128.
2D gel databases
SWISS-2DPAGE	P05976.
Proteomic databases
PaxDb	P05976.
PRIDE	P05976.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000341685; ENSP00000343321; ENSG00000168530. ENST00000352451; ENSP00000307280; ENSG00000168530.
GeneID	4632.
KEGG	hsa:4632.
UCSC	uc002veb.3. human. uc002vec.3. human.
Organism-specific databases
CTD	4632.
GeneCards	GC02M211118.
HGNC	HGNC:7582. MYL1.
MIM	160780. gene.
neXtProt	NX_P05976.
PharmGKB	PA31379.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5126.
HOGENOM	HOG000233018.
HOVERGEN	HBG012180.
InParanoid	P05976.
KO	K05738.
OMA	SKEQQDD.
Enzyme and pathway databases
Reactome	REACT_17044. Muscle contraction.
Gene expression databases
ArrayExpress	P05976.
Bgee	P05976.
CleanEx	HS_MYL1.
Genevestigator	P05976.
GermOnline	ENSG00000168530. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13405. EF_hand_4. 1 hit. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4632.
NextBio	17830.
SOURCE	Search...

Entry information

Entry name

MYL1_HUMAN

Accession

Primary (citable) accession number: P05976
Secondary accession number(s): B2R4N6 , B2R4T6, P06741, Q6IBD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.