P05964 (S10A6_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A6

Alternative name(s):
Calcyclin
Prolactin receptor-associated protein
S100 calcium-binding protein A6

Gene names

Name:	S100a6
Synonyms:	Cacy

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	89 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as calcium sensor and contribute to cellular calcium signaling Potential. May function by interacting with other proteins and indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative By similarity. Interacts with FKBP4 By similarity.
Subunit structure	Homodimer; head to tail assembly of 2 subunits. Interacts with ANXA2 and ANXA11 (via N-terminus). Interacts with SUGT1 and CACYBP. Interacts with TP53. Interacts with tropomyosin By similarity. Interacts with CACYBP in a calcium-dependent manner By similarity.
Subcellular location	Nucleus envelope By similarity. Cytoplasm By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Miscellaneous	This protein co-purified with the prolactin receptor.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Domain	Repeat
Ligand	Calcium Metal-binding
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB extrinsic to internal side of plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB nuclear envelope Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara ruffle Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB ion transmembrane transporter activity Inferred from direct assay PubMed 12000747. Source: RGD zinc ion binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 89	89	Protein S100-A6		PRO_0000143987
Regions
	Domain	12 – 47	36	EF-hand 1
	Domain	48 – 83	36	EF-hand 2
	Calcium binding	20 – 33	14	1 By similarity
	Calcium binding	61 – 72	12	2 By similarity
Amino acid modifications
	Modified residue	40	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	89	1	K → KG no nucleotide entry Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P05964 [UniParc]. Last modified April 13, 2004. Version 3. Checksum: 2AA1A4163D57DC87	FASTA	89	10,035
	10 20 30 40 50 60 MACPLDQAIG LLVAIFHKYS GKEGDKHTLS KKELKELIQK ELTIGAKLQD AEIARLMDDL 70 80 DRNKDQEVNF QEYVAFLGAL ALIYNEALK

References

[1]	"Cloning and characterization of a cDNA encoding a highly conserved, putative calcium binding protein, identified by an anti-prolactin receptor antiserum." Murphy L.C., Murphy L.J., Tsuyuki D., Duckworth M.L., Shiu R.P.C. J. Biol. Chem. 263:2397-2401(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Calcyclin is differentially expressed in rat testicular cells." Konrad L., Aumueller G. Biochim. Biophys. Acta 1489:440-444(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[3]	Kizawa K. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[4]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 48-55, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ132717 mRNA. Translation: CAB42002.1. AF140232 mRNA. Translation: AAK28306.1.
IPI	IPI00204818.
PIR	B28363.
RefSeq	NP_445937.1. NM_053485.2.
UniGene	Rn.3233.
3D structure databases
ProteinModelPortal	P05964.
SMR	P05964. Positions 2-89.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05964. 2 interactions.
Proteomic databases
PaxDb	P05964.
PRIDE	P05964.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000015612; ENSRNOP00000015612; ENSRNOG00000011647.
GeneID	85247.
KEGG	rno:85247.
UCSC	RGD:620264. rat.
Organism-specific databases
CTD	6277.
RGD	620264. S100a6.
Phylogenomic databases
eggNOG	NOG40006.
GeneTree	ENSGT00690000102087.
HOGENOM	HOG000246968.
HOVERGEN	HBG001479.
InParanoid	P05964.
OMA	VVNFQEY.
OrthoDB	EOG4MSD0W.
Gene expression databases
ArrayExpress	P05964.
Genevestigator	P05964.
GermOnline	ENSRNOG00000011647. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR001751. S100/CaBP-9k_CS. IPR013787. S100_Ca-bd_sub. [Graphical view]
Pfam	PF01023. S_100. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. PS00303. S100_CABP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617322.

Entry information

Entry name

S10A6_RAT

Accession

Primary (citable) accession number: P05964
Secondary accession number(s): Q9R2B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 104 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program