P05942 (S10A4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A4

Alternative name(s):
Metastasin
Nerve growth factor-induced protein 42A
P9K
Placental calcium-binding protein
S100 calcium-binding protein A4

Gene names

Name:

S100a4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subunit structure	Homodimer. Interacts with PPFIBP1 in a calcium-dependent mode By similarity.
Induction	By nerve growth factor.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium Metal-binding
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara
Cellular_component	neuron projection Inferred from direct assay PubMed 15101091. Source: RGD nucleus Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	actin binding Inferred from direct assay PubMed 8878885. Source: RGD calcium ion binding Inferred from direct assay PubMed 2357224. Source: RGD calcium-dependent protein binding Inferred from direct assay PubMed 8878885. Source: RGD protein heterodimerization activity Traceable author statement PubMed 15856021. Source: RGD protein homodimerization activity Traceable author statement PubMed 15856021. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 101	100	Protein S100-A4		PRO_0000143979
Regions
	Domain	12 – 47	36	EF-hand 1
	Domain	50 – 85	36	EF-hand 2
	Calcium binding	20 – 33	14	1; low affinity Potential
	Calcium binding	63 – 74	12	2; high affinity Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	35	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05942 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: EA0619CEE4F487C1	FASTA	101	11,776
	10 20 30 40 50 60 MARPLEEALD VIVSTFHKYS GNEGDKFKLN KTELKELLTR ELPSFLGRRT DEAAFQKLMN 70 80 90 100 NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGCPDKEPRK K

References

[1]	"Nerve growth factor induces the genes for two proteins related to a family of calcium-binding proteins in PC12 cells." Masiakowski P., Shooter E.M. Proc. Natl. Acad. Sci. U.S.A. 85:1277-1281(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and sequence of the gene for p9Ka. A cultured myoepithelial cell protein with strong homology to S-100, a calcium-binding protein." Barraclough R., Savin J., Dube S.K., Rudland P.S. J. Mol. Biol. 198:13-20(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Transformation of normal rat kidney cells by v-K-ras enhances expression of transin 2 and an S-100-related calcium-binding protein." de Vouge M.W., Mukherjee B.B. Oncogene 7:109-119(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 3-101.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06916 Genomic DNA. Translation: CAA30014.1. J03628 mRNA. Translation: AAA42098.1. X64022 mRNA. No translation available. X64023 mRNA. No translation available.
IPI	IPI00204748.
PIR	S01759.
RefSeq	NP_036750.1. NM_012618.2.
UniGene	Rn.504.
3D structure databases
ProteinModelPortal	P05942.
SMR	P05942. Positions 1-101.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000015958.
Proteomic databases
PaxDb	P05942.
PRIDE	P05942.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000015958; ENSRNOP00000015958; ENSRNOG00000011821.
GeneID	24615.
KEGG	rno:24615.
UCSC	RGD:3245. rat.
Organism-specific databases
CTD	6275.
RGD	3245. S100a4.
Phylogenomic databases
eggNOG	NOG40931.
GeneTree	ENSGT00690000102087.
HOGENOM	HOG000246968.
HOVERGEN	HBG001479.
InParanoid	P05942.
OMA	NEFFEGC.
OrthoDB	EOG4NS3D1.
Gene expression databases
Genevestigator	P05942.
GermOnline	ENSRNOG00000011821. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR001751. S100/CaBP-9k_CS. IPR013787. S100_Ca-bd_sub. [Graphical view]
Pfam	PF01023. S_100. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. PS00303. S100_CABP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603862.

Entry information

Entry name

S10A4_RAT

Accession

Primary (citable) accession number: P05942

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program