Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calbindin

Gene

CALB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi24 – 35121Add
BLAST
Calcium bindingi111 – 122122Add
BLAST
Calcium bindingi155 – 166123Add
BLAST
Calcium bindingi199 – 210124Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. vitamin D binding Source: UniProtKB-KW
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. cellular response to organic substance Source: Ensembl
  2. cytosolic calcium ion homeostasis Source: Ensembl
  3. locomotory behavior Source: Ensembl
  4. long-term memory Source: UniProtKB
  5. metanephric collecting duct development Source: Ensembl
  6. metanephric connecting tubule development Source: Ensembl
  7. metanephric distal convoluted tubule development Source: Ensembl
  8. metanephric part of ureteric bud development Source: Ensembl
  9. regulation of synaptic plasticity Source: Ensembl
  10. retina layer formation Source: Ensembl
  11. short-term memory Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding, Vitamin D

Names & Taxonomyi

Protein namesi
Recommended name:
Calbindin
Alternative name(s):
Calbindin D28
D-28K
Vitamin D-dependent calcium-binding protein, avian-type
Gene namesi
Name:CALB1
Synonyms:CAB27
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:1434. CALB1.

Subcellular locationi

GO - Cellular componenti

  1. axon Source: UniProtKB
  2. cytosol Source: Ensembl
  3. dendrite Source: UniProtKB
  4. extracellular vesicular exosome Source: UniProtKB
  5. neuronal cell body Source: UniProtKB
  6. nucleus Source: UniProtKB
  7. synapse Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26026.

Polymorphism and mutation databases

BioMutaiCALB1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 261260CalbindinPRO_0000073472Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP05937.
PaxDbiP05937.
PeptideAtlasiP05937.
PRIDEiP05937.

PTM databases

PhosphoSiteiP05937.

Expressioni

Gene expression databases

BgeeiP05937.
CleanExiHS_CALB1.
ExpressionAtlasiP05937. baseline and differential.
GenevestigatoriP05937.

Organism-specific databases

HPAiCAB002138.
HPA023099.
HPA056734.

Interactioni

Subunit structurei

Interacts with RANBP9.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
IKBKGQ9Y6K93EBI-4286943,EBI-81279
TRPV5Q9NQA54EBI-4286943,EBI-751281

Protein-protein interaction databases

BioGridi107244. 5 interactions.
IntActiP05937. 5 interactions.
MINTiMINT-2834520.
STRINGi9606.ENSP00000265431.

Structurei

3D structure databases

ProteinModelPortaliP05937.
SMRiP05937. Positions 1-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 4636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini53 – 8836EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini98 – 13336EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini142 – 17736EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Domaini186 – 22136EF-hand 5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 76Interaction with RANBP9

Domaini

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

Sequence similaritiesi

Belongs to the calbindin family.Curated
Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG299305.
GeneTreeiENSGT00390000013118.
HOGENOMiHOG000231866.
HOVERGENiHBG000855.
InParanoidiP05937.
KOiK14757.
OMAiMLQVFDA.
OrthoDBiEOG71G9VB.
PhylomeDBiP05937.
TreeFamiTF325083.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR029634. Calbindin.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR19972:SF3. PTHR19972:SF3. 1 hit.
PfamiPF13405. EF-hand_6. 2 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P05937-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESHLQSSL ITASQFFEIW LHFDADGSGY LEGKELQNLI QELQQARKKA
60 70 80 90 100
GLELSPEMKT FVDQYGQRDD GKIGIVELAH VLPTEENFLL LFRCQQLKSC
110 120 130 140 150
EEFMKTWRKY DTDHSGFIET EELKNFLKDL LEKANKTVDD TKLAEYTDLM
160 170 180 190 200
LKLFDSNNDG KLELTEMARL LPVQENFLLK FQGIKMCGKE FNKAFELYDQ
210 220 230 240 250
DGNGYIDENE LDALLKDLCE KNKQDLDINN ITTYKKNIMA LSDGGKLYRT
260
DLALILCAGD N
Length:261
Mass (Da):30,025
Last modified:January 23, 2007 - v2
Checksum:i8ED7A09BCC5FE5E4
GO
Isoform 2 (identifier: P05937-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.

Note: No experimental confirmation available.

Show »
Length:204
Mass (Da):23,609
Checksum:i50B8050A3B9210E3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5757Missing in isoform 2. 1 PublicationVSP_055508Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06661 mRNA. Translation: CAA29860.1.
AF068862 Genomic DNA. Translation: AAC62230.1.
AC004612 Genomic DNA. Translation: AAC14672.1.
AK054881 mRNA. Translation: BAG51438.1.
AK312491 mRNA. Translation: BAG35393.1.
AK315959 mRNA. Translation: BAH14330.1.
AF049895 Genomic DNA. No translation available.
AC123779 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91665.1.
CH471060 Genomic DNA. Translation: EAW91666.1.
BC006478 mRNA. Translation: AAH06478.1.
BC020864 mRNA. Translation: AAH20864.1.
M19878 Genomic DNA. Translation: AAA98991.1.
M19879 Genomic DNA. Translation: AAA98992.1.
CCDSiCCDS6251.1. [P05937-1]
PIRiS00234.
RefSeqiNP_004920.1. NM_004929.3. [P05937-1]
UniGeneiHs.65425.

Genome annotation databases

EnsembliENST00000265431; ENSP00000265431; ENSG00000104327. [P05937-1]
ENST00000518457; ENSP00000429602; ENSG00000104327. [P05937-2]
GeneIDi793.
KEGGihsa:793.
UCSCiuc003yel.1. human. [P05937-1]

Polymorphism and mutation databases

BioMutaiCALB1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Wikipedia

Calbindin entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06661 mRNA. Translation: CAA29860.1.
AF068862 Genomic DNA. Translation: AAC62230.1.
AC004612 Genomic DNA. Translation: AAC14672.1.
AK054881 mRNA. Translation: BAG51438.1.
AK312491 mRNA. Translation: BAG35393.1.
AK315959 mRNA. Translation: BAH14330.1.
AF049895 Genomic DNA. No translation available.
AC123779 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91665.1.
CH471060 Genomic DNA. Translation: EAW91666.1.
BC006478 mRNA. Translation: AAH06478.1.
BC020864 mRNA. Translation: AAH20864.1.
M19878 Genomic DNA. Translation: AAA98991.1.
M19879 Genomic DNA. Translation: AAA98992.1.
CCDSiCCDS6251.1. [P05937-1]
PIRiS00234.
RefSeqiNP_004920.1. NM_004929.3. [P05937-1]
UniGeneiHs.65425.

3D structure databases

ProteinModelPortaliP05937.
SMRiP05937. Positions 1-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107244. 5 interactions.
IntActiP05937. 5 interactions.
MINTiMINT-2834520.
STRINGi9606.ENSP00000265431.

PTM databases

PhosphoSiteiP05937.

Polymorphism and mutation databases

BioMutaiCALB1.

Proteomic databases

MaxQBiP05937.
PaxDbiP05937.
PeptideAtlasiP05937.
PRIDEiP05937.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265431; ENSP00000265431; ENSG00000104327. [P05937-1]
ENST00000518457; ENSP00000429602; ENSG00000104327. [P05937-2]
GeneIDi793.
KEGGihsa:793.
UCSCiuc003yel.1. human. [P05937-1]

Organism-specific databases

CTDi793.
GeneCardsiGC08M091070.
HGNCiHGNC:1434. CALB1.
HPAiCAB002138.
HPA023099.
HPA056734.
MIMi114050. gene.
neXtProtiNX_P05937.
PharmGKBiPA26026.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG299305.
GeneTreeiENSGT00390000013118.
HOGENOMiHOG000231866.
HOVERGENiHBG000855.
InParanoidiP05937.
KOiK14757.
OMAiMLQVFDA.
OrthoDBiEOG71G9VB.
PhylomeDBiP05937.
TreeFamiTF325083.

Miscellaneous databases

GenomeRNAii793.
NextBioi3218.
PROiP05937.
SOURCEiSearch...

Gene expression databases

BgeeiP05937.
CleanExiHS_CALB1.
ExpressionAtlasiP05937. baseline and differential.
GenevestigatoriP05937.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR029634. Calbindin.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR19972:SF3. PTHR19972:SF3. 1 hit.
PfamiPF13405. EF-hand_6. 2 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human 27-kDa calbindin complementary DNA sequence. Evolutionary and functional implications."
    Parmentier M., Lawson D.E.M., Vassart G.
    Eur. J. Biochem. 170:207-215(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Amygdala, Cerebellum and Substantia nigra.
  3. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney and Lung.
  6. "The human calbindin 27-kDa gene: structural organization of the 5' and 3' regions, chromosomal assignment, and restriction fragment length polymorphism."
    Parmentier M., de Vijlder J.J.M., Muir E., Szpirer C., Islam M.Q., Geurts van Kessel A., Lawson D.E.M., Vassart G.
    Genomics 4:309-319(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42 AND 201-261.
    Tissue: Brain.
  7. "Calbindin D28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy."
    Lutz W., Frank E.M., Craig T.A., Thompson R., Venters R.A., Kojetin D., Cavanagh J., Kumar R.
    Biochem. Biophys. Res. Commun. 303:1186-1192(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RANBP9.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCALB1_HUMAN
AccessioniPrimary (citable) accession number: P05937
Secondary accession number(s): B2R696, B7Z9J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.