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P05937 (CALB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calbindin
Alternative name(s):
Calbindin D28
D-28K
Vitamin D-dependent calcium-binding protein, avian-type
Gene names
Name:CALB1
Synonyms:CAB27
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Subunit structure

Interacts with RANBP9. Ref.7

Domain

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

Sequence similarities

Belongs to the calbindin family.

Contains 5 EF-hand domains.

Ontologies

Keywords
   DomainRepeat
   LigandCalcium
Metal-binding
Vitamin D
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to organic substance

Inferred from electronic annotation. Source: Ensembl

cytosolic calcium ion homeostasis

Inferred from electronic annotation. Source: Ensembl

locomotory behavior

Inferred from electronic annotation. Source: Ensembl

long-term memory

Inferred from mutant phenotype PubMed 22796338. Source: UniProt

metanephric collecting duct development

Inferred from electronic annotation. Source: Ensembl

metanephric connecting tubule development

Inferred from electronic annotation. Source: Ensembl

metanephric distal convoluted tubule development

Inferred from electronic annotation. Source: Ensembl

metanephric part of ureteric bud development

Inferred from electronic annotation. Source: Ensembl

regulation of synaptic plasticity

Inferred from electronic annotation. Source: Ensembl

retina layer formation

Inferred from electronic annotation. Source: Ensembl

short-term memory

Inferred from mutant phenotype PubMed 22796338. Source: UniProt

   Cellular_componentaxon

Inferred from direct assay PubMed 22796338. Source: UniProt

cytosol

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from direct assay PubMed 22796338. Source: UniProt

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867PubMed 23376485. Source: UniProt

neuronal cell body

Inferred from direct assay PubMed 22796338. Source: UniProt

nucleus

Inferred from direct assay PubMed 16928804. Source: UniProtKB

synapse

Inferred from electronic annotation. Source: Ensembl

   Molecular_functioncalcium ion binding

Inferred from mutant phenotype PubMed 18359862. Source: UniProt

protein binding

Inferred from physical interaction PubMed 16763551PubMed 20098747. Source: IntAct

vitamin D binding

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from mutant phenotype PubMed 18359862. Source: UniProt

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 261260Calbindin
PRO_0000073472

Regions

Domain11 – 4636EF-hand 1
Domain53 – 8836EF-hand 2
Domain98 – 13336EF-hand 3
Domain142 – 17736EF-hand 4
Domain186 – 22136EF-hand 5
Calcium binding24 – 35121
Calcium binding111 – 122122
Calcium binding155 – 166123
Calcium binding199 – 210124
Region2 – 76Interaction with RANBP9

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Sequences

Sequence LengthMass (Da)Tools
P05937 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 8ED7A09BCC5FE5E4

FASTA26130,025
        10         20         30         40         50         60 
MAESHLQSSL ITASQFFEIW LHFDADGSGY LEGKELQNLI QELQQARKKA GLELSPEMKT 

        70         80         90        100        110        120 
FVDQYGQRDD GKIGIVELAH VLPTEENFLL LFRCQQLKSC EEFMKTWRKY DTDHSGFIET 

       130        140        150        160        170        180 
EELKNFLKDL LEKANKTVDD TKLAEYTDLM LKLFDSNNDG KLELTEMARL LPVQENFLLK 

       190        200        210        220        230        240 
FQGIKMCGKE FNKAFELYDQ DGNGYIDENE LDALLKDLCE KNKQDLDINN ITTYKKNIMA 

       250        260 
LSDGGKLYRT DLALILCAGD N 

« Hide

References

« Hide 'large scale' references
[1]"Human 27-kDa calbindin complementary DNA sequence. Evolutionary and functional implications."
Parmentier M., Lawson D.E.M., Vassart G.
Eur. J. Biochem. 170:207-215(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cerebellum and Substantia nigra.
[3]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney and Lung.
[6]"The human calbindin 27-kDa gene: structural organization of the 5' and 3' regions, chromosomal assignment, and restriction fragment length polymorphism."
Parmentier M., de Vijlder J.J.M., Muir E., Szpirer C., Islam M.Q., Geurts van Kessel A., Lawson D.E.M., Vassart G.
Genomics 4:309-319(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42 AND 201-261.
Tissue: Brain.
[7]"Calbindin D28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy."
Lutz W., Frank E.M., Craig T.A., Thompson R., Venters R.A., Kojetin D., Cavanagh J., Kumar R.
Biochem. Biophys. Res. Commun. 303:1186-1192(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RANBP9.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Web resources

Wikipedia

Calbindin entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06661 mRNA. Translation: CAA29860.1.
AF068862 Genomic DNA. Translation: AAC62230.1.
AC004612 Genomic DNA. Translation: AAC14672.1.
AK054881 mRNA. Translation: BAG51438.1.
AK312491 mRNA. Translation: BAG35393.1.
AF049895 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91666.1.
BC006478 mRNA. Translation: AAH06478.1.
BC020864 mRNA. Translation: AAH20864.1.
M19878 Genomic DNA. Translation: AAA98991.1.
M19879 Genomic DNA. Translation: AAA98992.1.
CCDSCCDS6251.1.
PIRS00234.
RefSeqNP_004920.1. NM_004929.2.
UniGeneHs.65425.

3D structure databases

ProteinModelPortalP05937.
SMRP05937. Positions 1-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107244. 5 interactions.
IntActP05937. 5 interactions.
MINTMINT-2834520.
STRING9606.ENSP00000265431.

PTM databases

PhosphoSiteP05937.

Proteomic databases

MaxQBP05937.
PaxDbP05937.
PeptideAtlasP05937.
PRIDEP05937.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265431; ENSP00000265431; ENSG00000104327.
GeneID793.
KEGGhsa:793.
UCSCuc003yel.1. human.

Organism-specific databases

CTD793.
GeneCardsGC08M091070.
HGNCHGNC:1434. CALB1.
HPACAB002138.
HPA023099.
MIM114050. gene.
neXtProtNX_P05937.
PharmGKBPA26026.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG299305.
HOGENOMHOG000231866.
HOVERGENHBG000855.
InParanoidP05937.
KOK14757.
OMAEENFLLF.
OrthoDBEOG71G9VB.
PhylomeDBP05937.
TreeFamTF325083.

Gene expression databases

ArrayExpressP05937.
BgeeP05937.
CleanExHS_CALB1.
GenevestigatorP05937.

Family and domain databases

Gene3D1.10.238.10. 3 hits.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF13405. EF-hand_6. 2 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 4 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi793.
NextBio3218.
PROP05937.
SOURCESearch...

Entry information

Entry nameCALB1_HUMAN
AccessionPrimary (citable) accession number: P05937
Secondary accession number(s): B2R696
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM