P05937 (CALB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calbindin

Alternative name(s):
Calbindin D28
D-28K
Vitamin D-dependent calcium-binding protein, avian-type

Gene names

Name:	CALB1
Synonyms:	CAB27

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Subunit structure	Interacts with RANBP9. Ref.7
Domain	This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
Sequence similarities	Belongs to the calbindin family. Contains 5 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium Metal-binding Vitamin D
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to organic substance Inferred from electronic annotation. Source: Compara learning or memory Inferred from electronic annotation. Source: Compara locomotory behavior Inferred from electronic annotation. Source: Compara metanephric collecting duct development Inferred from electronic annotation. Source: Compara metanephric connecting tubule development Inferred from electronic annotation. Source: Compara metanephric distal convoluted tubule development Inferred from electronic annotation. Source: Compara metanephric part of ureteric bud development Inferred from electronic annotation. Source: Compara regulation of synaptic plasticity Inferred from electronic annotation. Source: Compara retina layer formation Inferred from electronic annotation. Source: Compara
Cellular_component	axon Inferred from sequence or structural similarity. Source: BHF-UCL cytosol Inferred from electronic annotation. Source: Compara dendrite Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 16928804. Source: UniProtKB
Molecular_function	calcium ion binding Traceable author statement Ref.1. Source: ProtInc vitamin D binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IKBKG	Q9Y6K9	3	EBI-4286943,EBI-81279

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 261	260	Calbindin		PRO_0000073472
Regions
	Domain	11 – 46	36	EF-hand 1
	Domain	53 – 88	36	EF-hand 2
	Domain	98 – 133	36	EF-hand 3
	Domain	142 – 177	36	EF-hand 4
	Domain	186 – 221	36	EF-hand 5
	Calcium binding	24 – 35	12	1
	Calcium binding	111 – 122	12	2
	Calcium binding	155 – 166	12	3
	Calcium binding	199 – 210	12	4
	Region	2 – 7	6	Interaction with RANBP9
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05937 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8ED7A09BCC5FE5E4	FASTA	261	30,025
	10 20 30 40 50 60 MAESHLQSSL ITASQFFEIW LHFDADGSGY LEGKELQNLI QELQQARKKA GLELSPEMKT 70 80 90 100 110 120 FVDQYGQRDD GKIGIVELAH VLPTEENFLL LFRCQQLKSC EEFMKTWRKY DTDHSGFIET 130 140 150 160 170 180 EELKNFLKDL LEKANKTVDD TKLAEYTDLM LKLFDSNNDG KLELTEMARL LPVQENFLLK 190 200 210 220 230 240 FQGIKMCGKE FNKAFELYDQ DGNGYIDENE LDALLKDLCE KNKQDLDINN ITTYKKNIMA 250 260 LSDGGKLYRT DLALILCAGD N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human 27-kDa calbindin complementary DNA sequence. Evolutionary and functional implications." Parmentier M., Lawson D.E.M., Vassart G. Eur. J. Biochem. 170:207-215(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum and Substantia nigra.
[3]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Lung.
[6]	"The human calbindin 27-kDa gene: structural organization of the 5' and 3' regions, chromosomal assignment, and restriction fragment length polymorphism." Parmentier M., de Vijlder J.J.M., Muir E., Szpirer C., Islam M.Q., Geurts van Kessel A., Lawson D.E.M., Vassart G. Genomics 4:309-319(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42 AND 201-261. Tissue: Brain.
[7]	"Calbindin D28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy." Lutz W., Frank E.M., Craig T.A., Thompson R., Venters R.A., Kojetin D., Cavanagh J., Kumar R. Biochem. Biophys. Res. Commun. 303:1186-1192(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RANBP9.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

Wikipedia

Calbindin entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06661 mRNA. Translation: CAA29860.1. AF068862 Genomic DNA. Translation: AAC62230.1. AC004612 Genomic DNA. Translation: AAC14672.1. AK054881 mRNA. Translation: BAG51438.1. AK312491 mRNA. Translation: BAG35393.1. AF049895 Genomic DNA. No translation available. CH471060 Genomic DNA. Translation: EAW91666.1. BC006478 mRNA. Translation: AAH06478.1. BC020864 mRNA. Translation: AAH20864.1. M19878 Genomic DNA. Translation: AAA98991.1. M19879 Genomic DNA. Translation: AAA98992.1.
IPI	IPI00220361.
PIR	S00234.
RefSeq	NP_004920.1. NM_004929.2.
UniGene	Hs.65425.
3D structure databases
ProteinModelPortal	P05937.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05937. 2 interactions.
MINT	MINT-2834520.
STRING	9606.ENSP00000265431.
PTM databases
PhosphoSite	P05937.
Polymorphism databases
DMDM	115394.
Proteomic databases
PaxDb	P05937.
PeptideAtlas	P05937.
PRIDE	P05937.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265431; ENSP00000265431; ENSG00000104327.
GeneID	793.
KEGG	hsa:793.
UCSC	uc003yel.1. human.
Organism-specific databases
CTD	793.
GeneCards	GC08M091070.
HGNC	HGNC:1434. CALB1.
HPA	CAB002138. HPA023099.
MIM	114050. gene.
neXtProt	NX_P05937.
PharmGKB	PA26026.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG299305.
HOGENOM	HOG000231866.
HOVERGEN	HBG000855.
InParanoid	P05937.
KO	K14757.
OMA	EENFLLF.
OrthoDB	EOG48SGTQ.
Gene expression databases
ArrayExpress	P05937.
Bgee	P05937.
CleanEx	HS_CALB1.
Genevestigator	P05937.
GermOnline	ENSG00000104327. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 3 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13405. EF_hand_4. 2 hits. PF13499. EF_hand_5. 1 hit. [Graphical view]
SMART	SM00054. EFh. 4 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 4 hits. PS50222. EF_HAND_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	793.
NextBio	3218.
SOURCE	Search...

Entry information

Entry name

CALB1_HUMAN

Accession

Primary (citable) accession number: P05937
Secondary accession number(s): B2R696

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.