P05847 (TTDA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L(+)-tartrate dehydratase subunit alpha
Short name=L-TTD alpha
EC=4.2.1.32

Gene names

Name:	ttdA
Synonyms:	ygjA
Ordered Locus Names:	b3061, JW3033

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(R,R)-tartrate = oxaloacetate + H2O. Ref.1
Cofactor	Iron-sulfur.
Subunit structure	Tetramer of two alpha and two beta subunits Probable. Ref.1
Induction	Induced by tartrate, via ttdR. Ref.5
Sequence similarities	Belongs to the class-I fumarase family.
Sequence caution	The sequence AAA72573.1 differs from that shown. Reason: Frameshift at position 198.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biofilm formation Inferred from mutant phenotype. Source: EcoCyc
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW L(+)-tartrate dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
glpR	P0ACL0	1	EBI-1113137,EBI-1123685
ompA	P0A910	1	EBI-1113137,EBI-371347

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	L(+)-tartrate dehydratase subunit alpha		PRO_0000195663
Sites
	Metal binding	71	1	Iron-sulfur By similarity
	Metal binding	190	1	Iron-sulfur By similarity
	Metal binding	277	1	Iron-sulfur By similarity
Experimental info
	Sequence conflict	11	1	V → L Ref.1
	Sequence conflict	11	1	V → L Ref.2
	Sequence conflict	231 – 232	2	EL → DV Ref.1
	Sequence conflict	231 – 232	2	EL → DV Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05847 [UniParc]. Last modified August 29, 2003. Version 3. Checksum: CBE29FEB6C47DB81	FASTA	303	32,734
	10 20 30 40 50 60 MMSESNKQQA VNKLTEIVAN FTAMISTRMP DDVVDKLKQL KDAETSSMGK IIYHTMFDNM 70 80 90 100 110 120 QKAIDLNRPA CQDTGEIMFF VKVGSRFPLL GELQSILKQA VEEATVKAPL RHNAVEIFDE 130 140 150 160 170 180 VNTGKNTGSG VPWVTWDIIP DNDDAEIEVY MAGGGCTLPG RSKVLMPSEG YEGVVKFVFE 190 200 210 220 230 240 NISTLAVNAC PPVLVGVGIA TSVETAAVLS RKAILRPIGS RHPNPKAAEL ELRLEEGLNR 250 260 270 280 290 300 LGIGPQGLTG NSSVMGVHIE SAARHPSTIG VAVSTGCWAH RRGTLLVHAD LTFENLSHTR SAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the L-tartrate dehydratase genes (ttdA and ttdB) of Escherichia coli and evolutionary relationship with the class I fumarase genes." Reaney S.K., Begg C., Bungard S.I., Guest J.R. J. Gen. Microbiol. 139:1523-1530(1993) [PubMed: 8371115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, SUBUNIT. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Possible new genes as revealed by molecular analysis of a 5-kb Escherichia coli chromosomal region 5' to the rpsU-dnaG-rpoD macromolecular-synthesis operon." Nesin M., Lupski J.R., Svec P., Godson G.N. Gene 51:149-161(1987) [PubMed: 3297921] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Functional identification of ygiP as a positive regulator of the ttdA-ttdB-ygjE operon." Oshima T., Biville F. Microbiology 152:2129-2135(2006) [PubMed: 16804186] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L14781 Unassigned DNA. Translation: AAA03061.1. M16194 Genomic DNA. Translation: AAA72573.1. Frameshift. U28379 Genomic DNA. Translation: AAA89141.1. U00096 Genomic DNA. Translation: AAC76097.1. AP009048 Genomic DNA. Translation: BAE77112.1.
PIR	QQECRT. C65094.
RefSeq	NP_417533.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P05847.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05847. 6 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001624; EBESCP00000001624; EBESCG00000001339. EBESCT00000018261; EBESCP00000017552; EBESCG00000017316.
GeneID	947565.
GenomeReviews	Gene locus JW3033 in contig AP009048_GR. Gene locus b3061 in contig U00096_GR.
KEGG	ecj:JW3033. eco:b3061.
PATRIC	32121536. VBIEscCol129921_3155.
Organism-specific databases
EchoBASE	EB1156.
EcoGene	EG11168. ttdA.
Phylogenomic databases
eggNOG	COG1951.
GeneTree	EBGT00050000010386.
HOGENOM	HBG548096.
OMA	ESSARHP.
PhylomeDB	P05847.
ProtClustDB	PRK08230.
Enzyme and pathway databases
BioCyc	EcoCyc:TTDA-MONOMER. MetaCyc:TTDA-MONOMER.
Gene expression databases
Genevestigator	P05847.
Family and domain databases
InterPro	IPR004646. Fe-S_hydro-lyase_TtdA-typ_cat. [Graphical view]
KO	K03779.
Pfam	PF05681. Fumerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00722. TtdA_fumA_fumB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TTDA_ECOLI

Accession

Primary (citable) accession number: P05847
Secondary accession number(s): P33130, Q2M9E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	August 29, 2003
Last modified:	January 25, 2012
This is version 99 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program