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Protein

Transposon Tn7 transposition protein TnsC

Gene

tnsC

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

TnsC binds non-specifically to DNA in the presence of ATP. It is required for Tn7 transposition.
TnsABC + TnsD promote high-frequency insertion of Tn7 into a specific target site known as att-Tn7 whereas TnsABC + TnsE promote low-frequency insertion into many different sites.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi136 – 143ATPSequence analysis8
DNA bindingi388 – 407H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA recombination, Transposition

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transposon Tn7 transposition protein TnsC
Short name:
Protein E
Gene namesi
Name:tnsC
Encoded oniPlasmid R7211 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726131 – 555Transposon Tn7 transposition protein TnsCAdd BLAST555

Proteomic databases

PRIDEiP05846.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP05846. 1 interactor.

Structurei

Secondary structure

1555
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi510 – 515Combined sources6
Helixi521 – 526Combined sources6
Helixi534 – 540Combined sources7
Helixi547 – 550Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T0FX-ray1.85C/D503-555[»]
ProteinModelPortaliP05846.
SMRiP05846.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05846.

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR021542. Tn7_TnsC.
[Graphical view]
PfamiPF13401. AAA_22. 1 hit.
PF11426. Tn7_TnsC_Int. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P05846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATRIQAVY RDTGVEAYRD NPFIEALPPL QESVNSAASL KSSLQLTSSD
60 70 80 90 100
LQKSRVIRAH TICRIPDDYF QPLGTHLLLS ERISVMIRGG YVGRNPKTGD
110 120 130 140 150
LQKHLQNGYE RVQTGELETF RFEEARSTAQ SLLLIGCSGS GKTTSLHRIL
160 170 180 190 200
ATYPQVIYHR ELNVEQVVYL KIDCSHNGSL KEICLNFFRA LDRALGSNYE
210 220 230 240 250
RRYGLKRHGI ETMLALMSQI ANAHALGLLV IDEIQHLSRS RSGGSQEMLN
260 270 280 290 300
FFVTMVNIIG VPVMLIGTPK AREIFEADLR SARRGAGFGA IFWDPIQQTQ
310 320 330 340 350
RGKPNQEWIA FTDNLWQLQL LQRKDALLSD EVRDVWYELS QGVMDIVVKL
360 370 380 390 400
FVLAQLRALA LGNERITAGL LRQVYQDELK PVHPMLEALR SGIPERIARY
410 420 430 440 450
SDLVVPEIDK RLIQLQLDIA AIQEQTPEEK ALQELDTEDQ RHLYLMLKED
460 470 480 490 500
YDSSLLIPTI KKAFSQNPTM TRQKLLPLVL QWLMEGETVV SELEKPSKSK
510 520 530 540 550
KVSAIKVVKP SDWDSLPDTD LRYIYSQRQP EKTMHERLKG KGVIVDMASL

FKQAG
Length:555
Mass (Da):62,995
Last modified:January 1, 1990 - v2
Checksum:i3CDADC9C9BB5E135
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17693 Genomic DNA. Translation: CAA35685.1.
AP002527 Genomic DNA. Translation: BAB12611.1.
X04492 Genomic DNA. Translation: CAA28180.1.
M37392 Genomic DNA. Translation: AAA24681.1.
PIRiS12639.
RefSeqiNP_065318.1. NC_002525.1.
WP_001276994.1. NZ_LXIU01000334.1.

Genome annotation databases

GeneIDi1238712.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17693 Genomic DNA. Translation: CAA35685.1.
AP002527 Genomic DNA. Translation: BAB12611.1.
X04492 Genomic DNA. Translation: CAA28180.1.
M37392 Genomic DNA. Translation: AAA24681.1.
PIRiS12639.
RefSeqiNP_065318.1. NC_002525.1.
WP_001276994.1. NZ_LXIU01000334.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T0FX-ray1.85C/D503-555[»]
ProteinModelPortaliP05846.
SMRiP05846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP05846. 1 interactor.

Proteomic databases

PRIDEiP05846.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1238712.

Miscellaneous databases

EvolutionaryTraceiP05846.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR021542. Tn7_TnsC.
[Graphical view]
PfamiPF13401. AAA_22. 1 hit.
PF11426. Tn7_TnsC_Int. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTNSC_ECOLX
AccessioniPrimary (citable) accession number: P05846
Secondary accession number(s): Q7AJH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 1, 1990
Last modified: November 30, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.