ID   FEPA_ECOLI              Reviewed;         746 AA.
AC   P05825; P75722; P76821; P77093;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   24-JAN-2024, entry version 195.
DE   RecName: Full=Ferrienterobactin receptor;
DE   AltName: Full=Enterobactin outer-membrane receptor;
DE   Flags: Precursor;
GN   Name=fepA; Synonyms=fep, feuB; OrderedLocusNames=b0584, JW5086;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3015941; DOI=10.1016/s0021-9258(18)67457-5;
RA   Lundrigan M.D., Kadner R.J.;
RT   "Nucleotide sequence of the gene for the ferrienterochelin receptor FepA in
RT   Escherichia coli. Homology among outer membrane receptors that interact
RT   with TonB.";
RL   J. Biol. Chem. 261:10797-10801(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
RX   PubMed=2974033; DOI=10.1016/s0021-9258(18)37361-7;
RA   Pettis G.S., Brickman T.J., McIntosh M.A.;
RT   "Transcriptional mapping and nucleotide sequence of the Escherichia coli
RT   fepA-fes enterobactin region. Identification of a unique iron-regulated
RT   bidirectional promoter.";
RL   J. Biol. Chem. 263:18857-18863(1988).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 735-746.
RX   PubMed=2526281; DOI=10.1111/j.1365-2958.1989.tb00224.x;
RA   Armstrong S.K., Pettis G.S., Forrester L.J., McIntosh M.A.;
RT   "The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide
RT   sequence and membrane localization of its protein product.";
RL   Mol. Microbiol. 3:757-766(1989).
RN   [8]
RP   MOLECULAR ANALYSIS.
RX   PubMed=2201687; DOI=10.1016/s0021-9258(18)77336-5;
RA   Armstrong S.K., Francis C.L., McIntosh M.A.;
RT   "Molecular analysis of the Escherichia coli ferric enterobactin receptor
RT   FepA.";
RL   J. Biol. Chem. 265:14536-14543(1990).
RN   [9]
RP   IDENTIFICATION BY 2D-GEL.
RX   PubMed=9298644; DOI=10.1002/elps.1150180805;
RA   VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT   "Escherichia coli proteome analysis using the gene-protein database.";
RL   Electrophoresis 18:1243-1251(1997).
RN   [10]
RP   INDUCTION BY HYDROXYUREA.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA   Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA   Walker G.C.;
RT   "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT   coli.";
RL   Mol. Cell 36:845-860(2009).
RN   [11]
RP   X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
RX   PubMed=9886293; DOI=10.1038/4931;
RA   Buchanan S.K., Smith B.S., Venkatramani L., Xia D., Esser L., Palnitkar M.,
RA   Chakraborty R., van der Helm D., Deisenhofer J.;
RT   "Crystal structure of the outer membrane active transporter FepA from
RT   Escherichia coli.";
RL   Nat. Struct. Biol. 6:56-63(1999).
CC   -!- FUNCTION: This protein is involved in the initial step of iron uptake
CC       by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore
CC       that allows E.coli to extract iron from the environment. FepA also acts
CC       as a receptor for colicins B and D.
CC   -!- INTERACTION:
CC       P05825; P02929: tonB; NbExp=2; IntAct=EBI-6400027, EBI-6399993;
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU01360}; Multi-pass membrane protein {ECO:0000255|PROSITE-
CC       ProRule:PRU01360}.
CC   -!- INDUCTION: Induced 1.9-fold by hydroxyurea.
CC       {ECO:0000269|PubMed:20005847}.
CC   -!- SIMILARITY: Belongs to the TonB-dependent receptor family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB40783.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; M13748; AAA65994.1; -; Genomic_DNA.
DR   EMBL; U82598; AAB40783.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC73685.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35225.1; -; Genomic_DNA.
DR   EMBL; J04216; AAA23756.1; -; Genomic_DNA.
DR   PIR; F64791; QRECFC.
DR   RefSeq; NP_415116.1; NC_000913.3.
DR   RefSeq; WP_001034943.1; NZ_LN832404.1.
DR   PDB; 1FEP; X-ray; 2.40 A; A=23-746.
DR   PDBsum; 1FEP; -.
DR   AlphaFoldDB; P05825; -.
DR   PCDDB; P05825; -.
DR   SMR; P05825; -.
DR   BioGRID; 4263074; 241.
DR   ComplexPortal; CPX-3578; Ferric-enterobactin outer membrane transporter complex.
DR   DIP; DIP-9592N; -.
DR   IntAct; P05825; 1.
DR   STRING; 511145.b0584; -.
DR   TCDB; 1.B.14.1.22; the outer membrane receptor (omr) family.
DR   jPOST; P05825; -.
DR   PaxDb; 511145-b0584; -.
DR   EnsemblBacteria; AAC73685; AAC73685; b0584.
DR   GeneID; 945193; -.
DR   KEGG; ecj:JW5086; -.
DR   KEGG; eco:b0584; -.
DR   PATRIC; fig|1411691.4.peg.1688; -.
DR   EchoBASE; EB0289; -.
DR   eggNOG; COG4771; Bacteria.
DR   HOGENOM; CLU_008287_18_2_6; -.
DR   InParanoid; P05825; -.
DR   OMA; NESGRTW; -.
DR   OrthoDB; 9764669at2; -.
DR   PhylomeDB; P05825; -.
DR   BioCyc; EcoCyc:EG10293-MONOMER; -.
DR   BioCyc; MetaCyc:EG10293-MONOMER; -.
DR   EvolutionaryTrace; P05825; -.
DR   PRO; PR:P05825; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0030313; C:cell envelope; IDA:CACAO.
DR   GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
DR   GO; GO:0016020; C:membrane; NAS:ComplexPortal.
DR   GO; GO:1902495; C:transmembrane transporter complex; NAS:ComplexPortal.
DR   GO; GO:0042912; F:colicin transmembrane transporter activity; IDA:EcoCyc.
DR   GO; GO:1903981; F:enterobactin binding; IDA:UniProtKB.
DR   GO; GO:0042931; F:enterobactin transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015620; F:ferric-enterobactin transmembrane transporter activity; IDA:EcoCyc.
DR   GO; GO:0022834; F:ligand-gated channel activity; IMP:EcoCyc.
DR   GO; GO:0019904; F:protein domain specific binding; IPI:CAFA.
DR   GO; GO:0015344; F:siderophore uptake transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0038023; F:signaling receptor activity; IEA:InterPro.
DR   GO; GO:0042914; P:colicin transport; IDA:EcoCyc.
DR   GO; GO:0042930; P:enterobactin transport; IBA:GO_Central.
DR   GO; GO:0015685; P:ferric-enterobactin import into cell; IDA:EcoCyc.
DR   GO; GO:0006879; P:intracellular iron ion homeostasis; NAS:ComplexPortal.
DR   GO; GO:0044718; P:siderophore transmembrane transport; IBA:GO_Central.
DR   GO; GO:0033214; P:siderophore-dependent iron import into cell; IMP:EcoCyc.
DR   CDD; cd01347; ligand_gated_channel; 1.
DR   Gene3D; 2.40.170.20; TonB-dependent receptor, beta-barrel domain; 1.
DR   Gene3D; 2.170.130.10; TonB-dependent receptor, plug domain; 1.
DR   InterPro; IPR012910; Plug_dom.
DR   InterPro; IPR037066; Plug_dom_sf.
DR   InterPro; IPR039426; TonB-dep_rcpt-like.
DR   InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR   InterPro; IPR010916; TonB_box_CS.
DR   InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR   InterPro; IPR010917; TonB_rcpt_CS.
DR   InterPro; IPR010105; TonB_sidphr_rcpt.
DR   NCBIfam; TIGR01783; TonB-siderophor; 1.
DR   PANTHER; PTHR30069:SF51; FERRIENTEROBACTIN RECEPTOR; 1.
DR   PANTHER; PTHR30069; TONB-DEPENDENT OUTER MEMBRANE RECEPTOR; 1.
DR   Pfam; PF07715; Plug; 1.
DR   Pfam; PF00593; TonB_dep_Rec; 1.
DR   SUPFAM; SSF56935; Porins; 1.
DR   PROSITE; PS00430; TONB_DEPENDENT_REC_1; 1.
DR   PROSITE; PS01156; TONB_DEPENDENT_REC_2; 1.
DR   PROSITE; PS52016; TONB_DEPENDENT_REC_3; 1.
DR   SWISS-2DPAGE; P05825; -.
PE   1: Evidence at protein level;
KW   3D-structure; Cell outer membrane; Ion transport; Iron; Iron transport;
KW   Membrane; Receptor; Reference proteome; Signal; TonB box; Transmembrane;
KW   Transmembrane beta strand; Transport.
FT   SIGNAL          1..22
FT   CHAIN           23..746
FT                   /note="Ferrienterobactin receptor"
FT                   /id="PRO_0000034747"
FT   DOMAIN          42..169
FT                   /note="TBDR plug"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01360"
FT   DOMAIN          174..746
FT                   /note="TBDR beta-barrel"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01360"
FT   REGION          76..96
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           34..41
FT                   /note="TonB box"
FT   MOTIF           729..746
FT                   /note="TonB C-terminal box"
FT   COMPBIAS        76..92
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        152
FT                   /note="A -> R (in Ref. 1; AAA65994)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        403
FT                   /note="Missing (in Ref. 1; AAA65994)"
FT                   /evidence="ECO:0000305"
FT   HELIX           40..44
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          50..54
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   HELIX           55..60
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   HELIX           68..71
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          77..82
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   TURN            87..90
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          92..96
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   HELIX           101..103
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          104..108
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   HELIX           115..118
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   HELIX           138..140
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          141..148
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   HELIX           150..153
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          160..168
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          176..188
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          194..204
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          206..220
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   TURN            225..231
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   HELIX           237..239
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          249..264
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          267..281
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   HELIX           294..298
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          304..318
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          324..338
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          360..397
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          426..441
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          443..457
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   TURN            458..460
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          461..473
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          478..489
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   TURN            493..496
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          501..503
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          517..519
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          527..540
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          543..560
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          573..597
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          600..614
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   TURN            615..617
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          626..638
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          641..650
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   HELIX           667..670
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          676..686
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          688..699
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          719..723
FT                   /evidence="ECO:0007829|PDB:1FEP"
FT   STRAND          737..746
FT                   /evidence="ECO:0007829|PDB:1FEP"
SQ   SEQUENCE   746 AA;  82107 MW;  09348AAB1C29710A CRC64;
     MNKKIHSLAL LVNLGIYGVA QAQEPTDTPV SHDDTIVVTA AEQNLQAPGV STITADEIRK
     NPVARDVSKI IRTMPGVNLT GNSTSGQRGN NRQIDIRGMG PENTLILIDG KPVSSRNSVR
     QGWRGERDTR GDTSWVPPEM IERIEVLRGP AAARYGNGAA GGVVNIITKK GSGEWHGSWD
     AYFNAPEHKE EGATKRTNFS LTGPLGDEFS FRLYGNLDKT QADAWDINQG HQSARAGTYA
     TTLPAGREGV INKDINGVVR WDFAPLQSLE LEAGYSRQGN LYAGDTQNTN SDSYTRSKYG
     DETNRLYRQN YALTWNGGWD NGVTTSNWVQ YEHTRNSRIP EGLAGGTEGK FNEKATQDFV
     DIDLDDVMLH SEVNLPIDFL VNQTLTLGTE WNQQRMKDLS SNTQALTGTN TGGAIDGVST
     TDRSPYSKAE IFSLFAENNM ELTDSTIVTP GLRFDHHSIV GNNWSPALNI SQGLGDDFTL
     KMGIARAYKA PSLYQTNPNY ILYSKGQGCY ASAGGCYLQG NDDLKAETSI NKEIGLEFKR
     DGWLAGVTWF RNDYRNKIEA GYVAVGQNAV GTDLYQWDNV PKAVVEGLEG SLNVPVSETV
     MWTNNITYML KSENKTTGDR LSIIPEYTLN STLSWQARED LSMQTTFTWY GKQQPKKYNY
     KGQPAVGPET KEISPYSIVG LSATWDVTKN VSLTGGVDNL FDKRLWRAGN AQTTGDLAGA
     NYIAGAGAYT YNEPGRTWYM SVNTHF
//