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Protein

Ferrienterobactin receptor

Gene

fepA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is involved in the initial step of iron uptake by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore that allows E.coli to extract iron from the environment. FepA also acts as a receptor for colicins B and D.

GO - Molecular functioni

  • colicin transmembrane transporter activity Source: EcoCyc
  • ferric-enterobactin transmembrane transporter activity Source: EcoCyc
  • iron ion binding Source: InterPro
  • ligand-gated channel activity Source: EcoCyc

GO - Biological processi

  • colicin transport Source: EcoCyc
  • ferric-enterobactin transport Source: EcoCyc
  • iron assimilation by chelation and transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:EG10293-MONOMER.
ECOL316407:JW5086-MONOMER.
MetaCyc:EG10293-MONOMER.

Protein family/group databases

TCDBi1.B.14.1.22. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrienterobactin receptor
Alternative name(s):
Enterobactin outer-membrane receptor
Gene namesi
Name:fepA
Synonyms:fep, feuB
Ordered Locus Names:b0584, JW5086
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10293. fepA.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of cell outer membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000003474723 – 746Ferrienterobactin receptorAdd BLAST724

Proteomic databases

EPDiP05825.
PaxDbiP05825.
PRIDEiP05825.

2D gel databases

SWISS-2DPAGEP05825.

Expressioni

Inductioni

Induced 1.9-fold by hydroxyurea.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tonBP029292EBI-6400027,EBI-6399993

Protein-protein interaction databases

BioGridi4263074. 237 interactors.
DIPiDIP-9592N.
IntActiP05825. 1 interactor.
STRINGi511145.b0584.

Structurei

Secondary structure

1746
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 44Combined sources5
Beta strandi50 – 54Combined sources5
Helixi55 – 60Combined sources6
Helixi68 – 71Combined sources4
Beta strandi77 – 82Combined sources6
Turni87 – 90Combined sources4
Beta strandi92 – 96Combined sources5
Helixi101 – 103Combined sources3
Beta strandi104 – 108Combined sources5
Helixi115 – 118Combined sources4
Helixi138 – 140Combined sources3
Beta strandi141 – 148Combined sources8
Helixi150 – 153Combined sources4
Beta strandi160 – 168Combined sources9
Beta strandi176 – 188Combined sources13
Beta strandi194 – 204Combined sources11
Beta strandi206 – 220Combined sources15
Turni225 – 231Combined sources7
Helixi237 – 239Combined sources3
Beta strandi249 – 264Combined sources16
Beta strandi267 – 281Combined sources15
Helixi294 – 298Combined sources5
Beta strandi304 – 318Combined sources15
Beta strandi324 – 338Combined sources15
Beta strandi360 – 397Combined sources38
Beta strandi426 – 441Combined sources16
Beta strandi443 – 457Combined sources15
Turni458 – 460Combined sources3
Beta strandi461 – 473Combined sources13
Beta strandi478 – 489Combined sources12
Turni493 – 496Combined sources4
Beta strandi501 – 503Combined sources3
Beta strandi517 – 519Combined sources3
Beta strandi527 – 540Combined sources14
Beta strandi543 – 560Combined sources18
Beta strandi573 – 597Combined sources25
Beta strandi600 – 614Combined sources15
Turni615 – 617Combined sources3
Beta strandi626 – 638Combined sources13
Beta strandi641 – 650Combined sources10
Helixi667 – 670Combined sources4
Beta strandi676 – 686Combined sources11
Beta strandi688 – 699Combined sources12
Beta strandi719 – 723Combined sources5
Beta strandi737 – 746Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FEPX-ray2.40A23-746[»]
ProteinModelPortaliP05825.
SMRiP05825.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05825.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 41TonB box8
Motifi729 – 746TonB C-terminal boxAdd BLAST18

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4107RNH. Bacteria.
COG4771. LUCA.
HOGENOMiHOG000276827.
InParanoidiP05825.
KOiK19611.
OMAiYRQNYAV.
PhylomeDBiP05825.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKIHSLAL LVNLGIYGVA QAQEPTDTPV SHDDTIVVTA AEQNLQAPGV
60 70 80 90 100
STITADEIRK NPVARDVSKI IRTMPGVNLT GNSTSGQRGN NRQIDIRGMG
110 120 130 140 150
PENTLILIDG KPVSSRNSVR QGWRGERDTR GDTSWVPPEM IERIEVLRGP
160 170 180 190 200
AAARYGNGAA GGVVNIITKK GSGEWHGSWD AYFNAPEHKE EGATKRTNFS
210 220 230 240 250
LTGPLGDEFS FRLYGNLDKT QADAWDINQG HQSARAGTYA TTLPAGREGV
260 270 280 290 300
INKDINGVVR WDFAPLQSLE LEAGYSRQGN LYAGDTQNTN SDSYTRSKYG
310 320 330 340 350
DETNRLYRQN YALTWNGGWD NGVTTSNWVQ YEHTRNSRIP EGLAGGTEGK
360 370 380 390 400
FNEKATQDFV DIDLDDVMLH SEVNLPIDFL VNQTLTLGTE WNQQRMKDLS
410 420 430 440 450
SNTQALTGTN TGGAIDGVST TDRSPYSKAE IFSLFAENNM ELTDSTIVTP
460 470 480 490 500
GLRFDHHSIV GNNWSPALNI SQGLGDDFTL KMGIARAYKA PSLYQTNPNY
510 520 530 540 550
ILYSKGQGCY ASAGGCYLQG NDDLKAETSI NKEIGLEFKR DGWLAGVTWF
560 570 580 590 600
RNDYRNKIEA GYVAVGQNAV GTDLYQWDNV PKAVVEGLEG SLNVPVSETV
610 620 630 640 650
MWTNNITYML KSENKTTGDR LSIIPEYTLN STLSWQARED LSMQTTFTWY
660 670 680 690 700
GKQQPKKYNY KGQPAVGPET KEISPYSIVG LSATWDVTKN VSLTGGVDNL
710 720 730 740
FDKRLWRAGN AQTTGDLAGA NYIAGAGAYT YNEPGRTWYM SVNTHF
Length:746
Mass (Da):82,107
Last modified:November 1, 1997 - v2
Checksum:i09348AAB1C29710A
GO

Sequence cautioni

The sequence AAB40783 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152A → R in AAA65994 (PubMed:3015941).Curated1
Sequence conflicti403Missing in AAA65994 (PubMed:3015941).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13748 Genomic DNA. Translation: AAA65994.1.
U82598 Genomic DNA. Translation: AAB40783.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73685.1.
AP009048 Genomic DNA. Translation: BAA35225.1.
J04216 Genomic DNA. Translation: AAA23756.1.
PIRiF64791. QRECFC.
RefSeqiNP_415116.1. NC_000913.3.
WP_001034943.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73685; AAC73685; b0584.
BAA35225; BAA35225; BAA35225.
GeneIDi945193.
KEGGiecj:JW5086.
eco:b0584.
PATRICi32116338. VBIEscCol129921_0609.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13748 Genomic DNA. Translation: AAA65994.1.
U82598 Genomic DNA. Translation: AAB40783.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73685.1.
AP009048 Genomic DNA. Translation: BAA35225.1.
J04216 Genomic DNA. Translation: AAA23756.1.
PIRiF64791. QRECFC.
RefSeqiNP_415116.1. NC_000913.3.
WP_001034943.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FEPX-ray2.40A23-746[»]
ProteinModelPortaliP05825.
SMRiP05825.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263074. 237 interactors.
DIPiDIP-9592N.
IntActiP05825. 1 interactor.
STRINGi511145.b0584.

Protein family/group databases

TCDBi1.B.14.1.22. the outer membrane receptor (omr) family.

2D gel databases

SWISS-2DPAGEP05825.

Proteomic databases

EPDiP05825.
PaxDbiP05825.
PRIDEiP05825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73685; AAC73685; b0584.
BAA35225; BAA35225; BAA35225.
GeneIDi945193.
KEGGiecj:JW5086.
eco:b0584.
PATRICi32116338. VBIEscCol129921_0609.

Organism-specific databases

EchoBASEiEB0289.
EcoGeneiEG10293. fepA.

Phylogenomic databases

eggNOGiENOG4107RNH. Bacteria.
COG4771. LUCA.
HOGENOMiHOG000276827.
InParanoidiP05825.
KOiK19611.
OMAiYRQNYAV.
PhylomeDBiP05825.

Enzyme and pathway databases

BioCyciEcoCyc:EG10293-MONOMER.
ECOL316407:JW5086-MONOMER.
MetaCyc:EG10293-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP05825.
PROiP05825.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFEPA_ECOLI
AccessioniPrimary (citable) accession number: P05825
Secondary accession number(s): P75722, P76821, P77093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.