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P05825 (FEPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ferrienterobactin receptor
Alternative name(s):
Enterobactin outer-membrane receptor
Gene names
Name:fepA
Synonyms:fep, feuB
Ordered Locus Names:b0584, JW5086
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length746 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is involved in the initial step of iron uptake by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore that allows E.coli to extract iron from the environment. FepA also acts as a receptor for colicins B and D.

Subcellular location

Cell outer membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the TonB-dependent receptor family.

Sequence caution

The sequence AAB40783.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

tonBP029292EBI-6400027,EBI-6399993

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222
Chain23 – 746724Ferrienterobactin receptor
PRO_0000034747

Regions

Motif34 – 418TonB box
Motif729 – 74618TonB C-terminal box

Experimental info

Sequence conflict1521A → R in AAA65994. Ref.1
Sequence conflict4031Missing in AAA65994. Ref.1

Secondary structure

.................................................................................... 746
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P05825 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 09348AAB1C29710A

FASTA74682,107
        10         20         30         40         50         60 
MNKKIHSLAL LVNLGIYGVA QAQEPTDTPV SHDDTIVVTA AEQNLQAPGV STITADEIRK 

        70         80         90        100        110        120 
NPVARDVSKI IRTMPGVNLT GNSTSGQRGN NRQIDIRGMG PENTLILIDG KPVSSRNSVR 

       130        140        150        160        170        180 
QGWRGERDTR GDTSWVPPEM IERIEVLRGP AAARYGNGAA GGVVNIITKK GSGEWHGSWD 

       190        200        210        220        230        240 
AYFNAPEHKE EGATKRTNFS LTGPLGDEFS FRLYGNLDKT QADAWDINQG HQSARAGTYA 

       250        260        270        280        290        300 
TTLPAGREGV INKDINGVVR WDFAPLQSLE LEAGYSRQGN LYAGDTQNTN SDSYTRSKYG 

       310        320        330        340        350        360 
DETNRLYRQN YALTWNGGWD NGVTTSNWVQ YEHTRNSRIP EGLAGGTEGK FNEKATQDFV 

       370        380        390        400        410        420 
DIDLDDVMLH SEVNLPIDFL VNQTLTLGTE WNQQRMKDLS SNTQALTGTN TGGAIDGVST 

       430        440        450        460        470        480 
TDRSPYSKAE IFSLFAENNM ELTDSTIVTP GLRFDHHSIV GNNWSPALNI SQGLGDDFTL 

       490        500        510        520        530        540 
KMGIARAYKA PSLYQTNPNY ILYSKGQGCY ASAGGCYLQG NDDLKAETSI NKEIGLEFKR 

       550        560        570        580        590        600 
DGWLAGVTWF RNDYRNKIEA GYVAVGQNAV GTDLYQWDNV PKAVVEGLEG SLNVPVSETV 

       610        620        630        640        650        660 
MWTNNITYML KSENKTTGDR LSIIPEYTLN STLSWQARED LSMQTTFTWY GKQQPKKYNY 

       670        680        690        700        710        720 
KGQPAVGPET KEISPYSIVG LSATWDVTKN VSLTGGVDNL FDKRLWRAGN AQTTGDLAGA 

       730        740 
NYIAGAGAYT YNEPGRTWYM SVNTHF 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the gene for the ferrienterochelin receptor FepA in Escherichia coli. Homology among outer membrane receptors that interact with TonB."
Lundrigan M.D., Kadner R.J.
J. Biol. Chem. 261:10797-10801(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter."
Pettis G.S., Brickman T.J., McIntosh M.A.
J. Biol. Chem. 263:18857-18863(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
[7]"The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product."
Armstrong S.K., Pettis G.S., Forrester L.J., McIntosh M.A.
Mol. Microbiol. 3:757-766(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 735-746.
[8]"Molecular analysis of the Escherichia coli ferric enterobactin receptor FepA."
Armstrong S.K., Francis C.L., McIntosh M.A.
J. Biol. Chem. 265:14536-14543(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: MOLECULAR ANALYSIS.
[9]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[10]"Crystal structure of the outer membrane active transporter FepA from Escherichia coli."
Buchanan S.K., Smith B.S., Venkatramani L., Xia D., Esser L., Palnitkar M., Chakraborty R., van der Helm D., Deisenhofer J.
Nat. Struct. Biol. 6:56-63(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13748 Genomic DNA. Translation: AAA65994.1.
U82598 Genomic DNA. Translation: AAB40783.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73685.1.
AP009048 Genomic DNA. Translation: BAA35225.1.
J04216 Genomic DNA. Translation: AAA23756.1.
PIRQRECFC. F64791.
RefSeqNP_415116.1. NC_000913.3.
YP_488872.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FEPX-ray2.40A23-746[»]
ProteinModelPortalP05825.
SMRP05825. Positions 33-746.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9592N.
IntActP05825. 1 interaction.
STRING511145.b0584.

Protein family/group databases

TCDB1.B.14.1.22. the outer membrane receptor (omr) family.

2D gel databases

SWISS-2DPAGEP05825.

Proteomic databases

PaxDbP05825.
PRIDEP05825.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73685; AAC73685; b0584.
BAA35225; BAA35225; BAA35225.
GeneID12934080.
945193.
KEGGecj:Y75_p0572.
eco:b0584.
PATRIC32116338. VBIEscCol129921_0609.

Organism-specific databases

EchoBASEEB0289.
EcoGeneEG10293. fepA.

Phylogenomic databases

eggNOGCOG4771.
HOGENOMHOG000276827.
KOK16089.
OMATTMLTPA.
OrthoDBEOG68H83J.
PhylomeDBP05825.
ProtClustDBPRK13524.

Enzyme and pathway databases

BioCycEcoCyc:EG10293-MONOMER.
ECOL316407:JW5086-MONOMER.

Gene expression databases

GenevestigatorP05825.

Family and domain databases

Gene3D2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProIPR012910. Plug.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsTIGR01783. TonB-siderophor. 1 hit.
PROSITEPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP05825.
PROP05825.

Entry information

Entry nameFEPA_ECOLI
AccessionPrimary (citable) accession number: P05825
Secondary accession number(s): P75722, P76821, P77093
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene