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P05825

- FEPA_ECOLI

UniProt

P05825 - FEPA_ECOLI

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Protein

Ferrienterobactin receptor

Gene

fepA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This protein is involved in the initial step of iron uptake by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore that allows E.coli to extract iron from the environment. FepA also acts as a receptor for colicins B and D.

GO - Molecular functioni

  1. colicin transmembrane transporter activity Source: EcoCyc
  2. ferric-enterobactin transmembrane transporter activity Source: EcoCyc
  3. iron ion binding Source: InterPro
  4. ligand-gated channel activity Source: EcoCyc
  5. receptor activity Source: InterPro

GO - Biological processi

  1. colicin transport Source: EcoCyc
  2. ferric-enterobactin transport Source: EcoCyc
  3. iron ion homeostasis Source: UniProtKB-KW
  4. transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:EG10293-MONOMER.
ECOL316407:JW5086-MONOMER.
MetaCyc:EG10293-MONOMER.

Protein family/group databases

TCDBi1.B.14.1.22. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrienterobactin receptor
Alternative name(s):
Enterobactin outer-membrane receptor
Gene namesi
Name:fepA
Synonyms:fep, feuB
Ordered Locus Names:b0584, JW5086
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10293. fepA.

Subcellular locationi

GO - Cellular componenti

  1. integral component of cell outer membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Add
BLAST
Chaini23 – 746724Ferrienterobactin receptorPRO_0000034747Add
BLAST

Proteomic databases

PaxDbiP05825.
PRIDEiP05825.

2D gel databases

SWISS-2DPAGEP05825.

Expressioni

Gene expression databases

GenevestigatoriP05825.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tonBP029292EBI-6400027,EBI-6399993

Protein-protein interaction databases

DIPiDIP-9592N.
IntActiP05825. 1 interaction.
STRINGi511145.b0584.

Structurei

Secondary structure

1
746
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 445Combined sources
Beta strandi50 – 545Combined sources
Helixi55 – 606Combined sources
Helixi68 – 714Combined sources
Beta strandi77 – 826Combined sources
Turni87 – 904Combined sources
Beta strandi92 – 965Combined sources
Helixi101 – 1033Combined sources
Beta strandi104 – 1085Combined sources
Helixi115 – 1184Combined sources
Helixi138 – 1403Combined sources
Beta strandi141 – 1488Combined sources
Helixi150 – 1534Combined sources
Beta strandi160 – 1689Combined sources
Beta strandi176 – 18813Combined sources
Beta strandi194 – 20411Combined sources
Beta strandi206 – 22015Combined sources
Turni225 – 2317Combined sources
Helixi237 – 2393Combined sources
Beta strandi249 – 26416Combined sources
Beta strandi267 – 28115Combined sources
Helixi294 – 2985Combined sources
Beta strandi304 – 31815Combined sources
Beta strandi324 – 33815Combined sources
Beta strandi360 – 39738Combined sources
Beta strandi426 – 44116Combined sources
Beta strandi443 – 45715Combined sources
Turni458 – 4603Combined sources
Beta strandi461 – 47313Combined sources
Beta strandi478 – 48912Combined sources
Turni493 – 4964Combined sources
Beta strandi501 – 5033Combined sources
Beta strandi517 – 5193Combined sources
Beta strandi527 – 54014Combined sources
Beta strandi543 – 56018Combined sources
Beta strandi573 – 59725Combined sources
Beta strandi600 – 61415Combined sources
Turni615 – 6173Combined sources
Beta strandi626 – 63813Combined sources
Beta strandi641 – 65010Combined sources
Helixi667 – 6704Combined sources
Beta strandi676 – 68611Combined sources
Beta strandi688 – 69912Combined sources
Beta strandi719 – 7235Combined sources
Beta strandi737 – 74610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FEPX-ray2.40A23-746[»]
ProteinModelPortaliP05825.
SMRiP05825. Positions 33-746.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05825.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 418TonB box
Motifi729 – 74618TonB C-terminal boxAdd
BLAST

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiCOG4771.
HOGENOMiHOG000276827.
InParanoidiP05825.
KOiK16089.
OMAiTNTYAQY.
OrthoDBiEOG68H83J.
PhylomeDBiP05825.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05825-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKKIHSLAL LVNLGIYGVA QAQEPTDTPV SHDDTIVVTA AEQNLQAPGV
60 70 80 90 100
STITADEIRK NPVARDVSKI IRTMPGVNLT GNSTSGQRGN NRQIDIRGMG
110 120 130 140 150
PENTLILIDG KPVSSRNSVR QGWRGERDTR GDTSWVPPEM IERIEVLRGP
160 170 180 190 200
AAARYGNGAA GGVVNIITKK GSGEWHGSWD AYFNAPEHKE EGATKRTNFS
210 220 230 240 250
LTGPLGDEFS FRLYGNLDKT QADAWDINQG HQSARAGTYA TTLPAGREGV
260 270 280 290 300
INKDINGVVR WDFAPLQSLE LEAGYSRQGN LYAGDTQNTN SDSYTRSKYG
310 320 330 340 350
DETNRLYRQN YALTWNGGWD NGVTTSNWVQ YEHTRNSRIP EGLAGGTEGK
360 370 380 390 400
FNEKATQDFV DIDLDDVMLH SEVNLPIDFL VNQTLTLGTE WNQQRMKDLS
410 420 430 440 450
SNTQALTGTN TGGAIDGVST TDRSPYSKAE IFSLFAENNM ELTDSTIVTP
460 470 480 490 500
GLRFDHHSIV GNNWSPALNI SQGLGDDFTL KMGIARAYKA PSLYQTNPNY
510 520 530 540 550
ILYSKGQGCY ASAGGCYLQG NDDLKAETSI NKEIGLEFKR DGWLAGVTWF
560 570 580 590 600
RNDYRNKIEA GYVAVGQNAV GTDLYQWDNV PKAVVEGLEG SLNVPVSETV
610 620 630 640 650
MWTNNITYML KSENKTTGDR LSIIPEYTLN STLSWQARED LSMQTTFTWY
660 670 680 690 700
GKQQPKKYNY KGQPAVGPET KEISPYSIVG LSATWDVTKN VSLTGGVDNL
710 720 730 740
FDKRLWRAGN AQTTGDLAGA NYIAGAGAYT YNEPGRTWYM SVNTHF
Length:746
Mass (Da):82,107
Last modified:November 1, 1997 - v2
Checksum:i09348AAB1C29710A
GO

Sequence cautioni

The sequence AAB40783.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521A → R in AAA65994. (PubMed:3015941)Curated
Sequence conflicti403 – 4031Missing in AAA65994. (PubMed:3015941)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13748 Genomic DNA. Translation: AAA65994.1.
U82598 Genomic DNA. Translation: AAB40783.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73685.1.
AP009048 Genomic DNA. Translation: BAA35225.1.
J04216 Genomic DNA. Translation: AAA23756.1.
PIRiF64791. QRECFC.
RefSeqiNP_415116.1. NC_000913.3.
YP_488872.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73685; AAC73685; b0584.
BAA35225; BAA35225; BAA35225.
GeneIDi12934080.
945193.
KEGGiecj:Y75_p0572.
eco:b0584.
PATRICi32116338. VBIEscCol129921_0609.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13748 Genomic DNA. Translation: AAA65994.1 .
U82598 Genomic DNA. Translation: AAB40783.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC73685.1 .
AP009048 Genomic DNA. Translation: BAA35225.1 .
J04216 Genomic DNA. Translation: AAA23756.1 .
PIRi F64791. QRECFC.
RefSeqi NP_415116.1. NC_000913.3.
YP_488872.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FEP X-ray 2.40 A 23-746 [» ]
ProteinModelPortali P05825.
SMRi P05825. Positions 33-746.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9592N.
IntActi P05825. 1 interaction.
STRINGi 511145.b0584.

Protein family/group databases

TCDBi 1.B.14.1.22. the outer membrane receptor (omr) family.

2D gel databases

SWISS-2DPAGE P05825.

Proteomic databases

PaxDbi P05825.
PRIDEi P05825.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73685 ; AAC73685 ; b0584 .
BAA35225 ; BAA35225 ; BAA35225 .
GeneIDi 12934080.
945193.
KEGGi ecj:Y75_p0572.
eco:b0584.
PATRICi 32116338. VBIEscCol129921_0609.

Organism-specific databases

EchoBASEi EB0289.
EcoGenei EG10293. fepA.

Phylogenomic databases

eggNOGi COG4771.
HOGENOMi HOG000276827.
InParanoidi P05825.
KOi K16089.
OMAi TNTYAQY.
OrthoDBi EOG68H83J.
PhylomeDBi P05825.

Enzyme and pathway databases

BioCyci EcoCyc:EG10293-MONOMER.
ECOL316407:JW5086-MONOMER.
MetaCyc:EG10293-MONOMER.

Miscellaneous databases

EvolutionaryTracei P05825.
PROi P05825.

Gene expression databases

Genevestigatori P05825.

Family and domain databases

Gene3Di 2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProi IPR012910. Plug.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view ]
Pfami PF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01783. TonB-siderophor. 1 hit.
PROSITEi PS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the gene for the ferrienterochelin receptor FepA in Escherichia coli. Homology among outer membrane receptors that interact with TonB."
    Lundrigan M.D., Kadner R.J.
    J. Biol. Chem. 261:10797-10801(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter."
    Pettis G.S., Brickman T.J., McIntosh M.A.
    J. Biol. Chem. 263:18857-18863(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
  7. "The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product."
    Armstrong S.K., Pettis G.S., Forrester L.J., McIntosh M.A.
    Mol. Microbiol. 3:757-766(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 735-746.
  8. "Molecular analysis of the Escherichia coli ferric enterobactin receptor FepA."
    Armstrong S.K., Francis C.L., McIntosh M.A.
    J. Biol. Chem. 265:14536-14543(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: MOLECULAR ANALYSIS.
  9. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  10. "Crystal structure of the outer membrane active transporter FepA from Escherichia coli."
    Buchanan S.K., Smith B.S., Venkatramani L., Xia D., Esser L., Palnitkar M., Chakraborty R., van der Helm D., Deisenhofer J.
    Nat. Struct. Biol. 6:56-63(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Entry informationi

Entry nameiFEPA_ECOLI
AccessioniPrimary (citable) accession number: P05825
Secondary accession number(s): P75722, P76821, P77093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3