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P05825

- FEPA_ECOLI

UniProt

P05825 - FEPA_ECOLI

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Protein
Ferrienterobactin receptor
Gene
fepA, fep, feuB, b0584, JW5086
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This protein is involved in the initial step of iron uptake by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore that allows E.coli to extract iron from the environment. FepA also acts as a receptor for colicins B and D.

GO - Molecular functioni

  1. colicin transmembrane transporter activity Source: EcoCyc
  2. ferric-enterobactin transmembrane transporter activity Source: EcoCyc
  3. iron ion binding Source: InterPro
  4. ligand-gated channel activity Source: EcoCyc
  5. protein binding Source: IntAct
  6. receptor activity Source: InterPro

GO - Biological processi

  1. colicin transport Source: EcoCyc
  2. ferric-enterobactin transport Source: EcoCyc
  3. transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:EG10293-MONOMER.
ECOL316407:JW5086-MONOMER.
MetaCyc:EG10293-MONOMER.

Protein family/group databases

TCDBi1.B.14.1.22. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrienterobactin receptor
Alternative name(s):
Enterobactin outer-membrane receptor
Gene namesi
Name:fepA
Synonyms:fep, feuB
Ordered Locus Names:b0584, JW5086
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10293. fepA.

Subcellular locationi

GO - Cellular componenti

  1. integral component of cell outer membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222
Add
BLAST
Chaini23 – 746724Ferrienterobactin receptor
PRO_0000034747Add
BLAST

Proteomic databases

PaxDbiP05825.
PRIDEiP05825.

2D gel databases

SWISS-2DPAGEP05825.

Expressioni

Gene expression databases

GenevestigatoriP05825.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tonBP029292EBI-6400027,EBI-6399993

Protein-protein interaction databases

DIPiDIP-9592N.
IntActiP05825. 1 interaction.
STRINGi511145.b0584.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 445
Beta strandi50 – 545
Helixi55 – 606
Helixi68 – 714
Beta strandi77 – 826
Turni87 – 904
Beta strandi92 – 965
Helixi101 – 1033
Beta strandi104 – 1085
Helixi115 – 1184
Helixi138 – 1403
Beta strandi141 – 1488
Helixi150 – 1534
Beta strandi160 – 1689
Beta strandi176 – 18813
Beta strandi194 – 20411
Beta strandi206 – 22015
Turni225 – 2317
Helixi237 – 2393
Beta strandi249 – 26416
Beta strandi267 – 28115
Helixi294 – 2985
Beta strandi304 – 31815
Beta strandi324 – 33815
Beta strandi360 – 39738
Beta strandi426 – 44116
Beta strandi443 – 45715
Turni458 – 4603
Beta strandi461 – 47313
Beta strandi478 – 48912
Turni493 – 4964
Beta strandi501 – 5033
Beta strandi517 – 5193
Beta strandi527 – 54014
Beta strandi543 – 56018
Beta strandi573 – 59725
Beta strandi600 – 61415
Turni615 – 6173
Beta strandi626 – 63813
Beta strandi641 – 65010
Helixi667 – 6704
Beta strandi676 – 68611
Beta strandi688 – 69912
Beta strandi719 – 7235
Beta strandi737 – 74610

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FEPX-ray2.40A23-746[»]
ProteinModelPortaliP05825.
SMRiP05825. Positions 33-746.

Miscellaneous databases

EvolutionaryTraceiP05825.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 418TonB box
Motifi729 – 74618TonB C-terminal box
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiCOG4771.
HOGENOMiHOG000276827.
KOiK16089.
OMAiTNTYAQY.
OrthoDBiEOG68H83J.
PhylomeDBiP05825.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05825-1 [UniParc]FASTAAdd to Basket

« Hide

MNKKIHSLAL LVNLGIYGVA QAQEPTDTPV SHDDTIVVTA AEQNLQAPGV    50
STITADEIRK NPVARDVSKI IRTMPGVNLT GNSTSGQRGN NRQIDIRGMG 100
PENTLILIDG KPVSSRNSVR QGWRGERDTR GDTSWVPPEM IERIEVLRGP 150
AAARYGNGAA GGVVNIITKK GSGEWHGSWD AYFNAPEHKE EGATKRTNFS 200
LTGPLGDEFS FRLYGNLDKT QADAWDINQG HQSARAGTYA TTLPAGREGV 250
INKDINGVVR WDFAPLQSLE LEAGYSRQGN LYAGDTQNTN SDSYTRSKYG 300
DETNRLYRQN YALTWNGGWD NGVTTSNWVQ YEHTRNSRIP EGLAGGTEGK 350
FNEKATQDFV DIDLDDVMLH SEVNLPIDFL VNQTLTLGTE WNQQRMKDLS 400
SNTQALTGTN TGGAIDGVST TDRSPYSKAE IFSLFAENNM ELTDSTIVTP 450
GLRFDHHSIV GNNWSPALNI SQGLGDDFTL KMGIARAYKA PSLYQTNPNY 500
ILYSKGQGCY ASAGGCYLQG NDDLKAETSI NKEIGLEFKR DGWLAGVTWF 550
RNDYRNKIEA GYVAVGQNAV GTDLYQWDNV PKAVVEGLEG SLNVPVSETV 600
MWTNNITYML KSENKTTGDR LSIIPEYTLN STLSWQARED LSMQTTFTWY 650
GKQQPKKYNY KGQPAVGPET KEISPYSIVG LSATWDVTKN VSLTGGVDNL 700
FDKRLWRAGN AQTTGDLAGA NYIAGAGAYT YNEPGRTWYM SVNTHF 746
Length:746
Mass (Da):82,107
Last modified:November 1, 1997 - v2
Checksum:i09348AAB1C29710A
GO

Sequence cautioni

The sequence AAB40783.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521A → R in AAA65994. 1 Publication
Sequence conflicti403 – 4031Missing in AAA65994. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13748 Genomic DNA. Translation: AAA65994.1.
U82598 Genomic DNA. Translation: AAB40783.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73685.1.
AP009048 Genomic DNA. Translation: BAA35225.1.
J04216 Genomic DNA. Translation: AAA23756.1.
PIRiF64791. QRECFC.
RefSeqiNP_415116.1. NC_000913.3.
YP_488872.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73685; AAC73685; b0584.
BAA35225; BAA35225; BAA35225.
GeneIDi12934080.
945193.
KEGGiecj:Y75_p0572.
eco:b0584.
PATRICi32116338. VBIEscCol129921_0609.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13748 Genomic DNA. Translation: AAA65994.1 .
U82598 Genomic DNA. Translation: AAB40783.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC73685.1 .
AP009048 Genomic DNA. Translation: BAA35225.1 .
J04216 Genomic DNA. Translation: AAA23756.1 .
PIRi F64791. QRECFC.
RefSeqi NP_415116.1. NC_000913.3.
YP_488872.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FEP X-ray 2.40 A 23-746 [» ]
ProteinModelPortali P05825.
SMRi P05825. Positions 33-746.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9592N.
IntActi P05825. 1 interaction.
STRINGi 511145.b0584.

Protein family/group databases

TCDBi 1.B.14.1.22. the outer membrane receptor (omr) family.

2D gel databases

SWISS-2DPAGE P05825.

Proteomic databases

PaxDbi P05825.
PRIDEi P05825.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73685 ; AAC73685 ; b0584 .
BAA35225 ; BAA35225 ; BAA35225 .
GeneIDi 12934080.
945193.
KEGGi ecj:Y75_p0572.
eco:b0584.
PATRICi 32116338. VBIEscCol129921_0609.

Organism-specific databases

EchoBASEi EB0289.
EcoGenei EG10293. fepA.

Phylogenomic databases

eggNOGi COG4771.
HOGENOMi HOG000276827.
KOi K16089.
OMAi TNTYAQY.
OrthoDBi EOG68H83J.
PhylomeDBi P05825.

Enzyme and pathway databases

BioCyci EcoCyc:EG10293-MONOMER.
ECOL316407:JW5086-MONOMER.
MetaCyc:EG10293-MONOMER.

Miscellaneous databases

EvolutionaryTracei P05825.
PROi P05825.

Gene expression databases

Genevestigatori P05825.

Family and domain databases

Gene3Di 2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProi IPR012910. Plug.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view ]
Pfami PF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01783. TonB-siderophor. 1 hit.
PROSITEi PS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the gene for the ferrienterochelin receptor FepA in Escherichia coli. Homology among outer membrane receptors that interact with TonB."
    Lundrigan M.D., Kadner R.J.
    J. Biol. Chem. 261:10797-10801(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter."
    Pettis G.S., Brickman T.J., McIntosh M.A.
    J. Biol. Chem. 263:18857-18863(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
  7. "The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product."
    Armstrong S.K., Pettis G.S., Forrester L.J., McIntosh M.A.
    Mol. Microbiol. 3:757-766(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 735-746.
  8. "Molecular analysis of the Escherichia coli ferric enterobactin receptor FepA."
    Armstrong S.K., Francis C.L., McIntosh M.A.
    J. Biol. Chem. 265:14536-14543(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: MOLECULAR ANALYSIS.
  9. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  10. "Crystal structure of the outer membrane active transporter FepA from Escherichia coli."
    Buchanan S.K., Smith B.S., Venkatramani L., Xia D., Esser L., Palnitkar M., Chakraborty R., van der Helm D., Deisenhofer J.
    Nat. Struct. Biol. 6:56-63(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Entry informationi

Entry nameiFEPA_ECOLI
AccessioniPrimary (citable) accession number: P05825
Secondary accession number(s): P75722, P76821, P77093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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