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P05825

- FEPA_ECOLI

UniProt

P05825 - FEPA_ECOLI

Protein

Ferrienterobactin receptor

Gene

fepA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    This protein is involved in the initial step of iron uptake by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore that allows E.coli to extract iron from the environment. FepA also acts as a receptor for colicins B and D.

    GO - Molecular functioni

    1. colicin transmembrane transporter activity Source: EcoCyc
    2. ferric-enterobactin transmembrane transporter activity Source: EcoCyc
    3. iron ion binding Source: InterPro
    4. ligand-gated channel activity Source: EcoCyc
    5. protein binding Source: IntAct
    6. receptor activity Source: InterPro

    GO - Biological processi

    1. colicin transport Source: EcoCyc
    2. ferric-enterobactin transport Source: EcoCyc
    3. transmembrane transport Source: GOC

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Ion transport, Iron transport, Transport

    Keywords - Ligandi

    Iron

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10293-MONOMER.
    ECOL316407:JW5086-MONOMER.
    MetaCyc:EG10293-MONOMER.

    Protein family/group databases

    TCDBi1.B.14.1.22. the outer membrane receptor (omr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ferrienterobactin receptor
    Alternative name(s):
    Enterobactin outer-membrane receptor
    Gene namesi
    Name:fepA
    Synonyms:fep, feuB
    Ordered Locus Names:b0584, JW5086
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10293. fepA.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of cell outer membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Add
    BLAST
    Chaini23 – 746724Ferrienterobactin receptorPRO_0000034747Add
    BLAST

    Proteomic databases

    PaxDbiP05825.
    PRIDEiP05825.

    2D gel databases

    SWISS-2DPAGEP05825.

    Expressioni

    Gene expression databases

    GenevestigatoriP05825.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    tonBP029292EBI-6400027,EBI-6399993

    Protein-protein interaction databases

    DIPiDIP-9592N.
    IntActiP05825. 1 interaction.
    STRINGi511145.b0584.

    Structurei

    Secondary structure

    1
    746
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi40 – 445
    Beta strandi50 – 545
    Helixi55 – 606
    Helixi68 – 714
    Beta strandi77 – 826
    Turni87 – 904
    Beta strandi92 – 965
    Helixi101 – 1033
    Beta strandi104 – 1085
    Helixi115 – 1184
    Helixi138 – 1403
    Beta strandi141 – 1488
    Helixi150 – 1534
    Beta strandi160 – 1689
    Beta strandi176 – 18813
    Beta strandi194 – 20411
    Beta strandi206 – 22015
    Turni225 – 2317
    Helixi237 – 2393
    Beta strandi249 – 26416
    Beta strandi267 – 28115
    Helixi294 – 2985
    Beta strandi304 – 31815
    Beta strandi324 – 33815
    Beta strandi360 – 39738
    Beta strandi426 – 44116
    Beta strandi443 – 45715
    Turni458 – 4603
    Beta strandi461 – 47313
    Beta strandi478 – 48912
    Turni493 – 4964
    Beta strandi501 – 5033
    Beta strandi517 – 5193
    Beta strandi527 – 54014
    Beta strandi543 – 56018
    Beta strandi573 – 59725
    Beta strandi600 – 61415
    Turni615 – 6173
    Beta strandi626 – 63813
    Beta strandi641 – 65010
    Helixi667 – 6704
    Beta strandi676 – 68611
    Beta strandi688 – 69912
    Beta strandi719 – 7235
    Beta strandi737 – 74610

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FEPX-ray2.40A23-746[»]
    ProteinModelPortaliP05825.
    SMRiP05825. Positions 33-746.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05825.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi34 – 418TonB box
    Motifi729 – 74618TonB C-terminal boxAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TonB-dependent receptor family.Curated

    Keywords - Domaini

    Signal, TonB box, Transmembrane, Transmembrane beta strand

    Phylogenomic databases

    eggNOGiCOG4771.
    HOGENOMiHOG000276827.
    KOiK16089.
    OMAiTNTYAQY.
    OrthoDBiEOG68H83J.
    PhylomeDBiP05825.

    Family and domain databases

    Gene3Di2.170.130.10. 1 hit.
    2.40.170.20. 1 hit.
    InterProiIPR012910. Plug.
    IPR000531. TonB-dep_rcpt_b-brl.
    IPR010916. TonB_box_CS.
    IPR010917. TonB_rcpt_CS.
    IPR010105. TonB_sidphr_rcpt.
    [Graphical view]
    PfamiPF07715. Plug. 1 hit.
    PF00593. TonB_dep_Rec. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
    PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
    PS01156. TONB_DEPENDENT_REC_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05825-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKKIHSLAL LVNLGIYGVA QAQEPTDTPV SHDDTIVVTA AEQNLQAPGV    50
    STITADEIRK NPVARDVSKI IRTMPGVNLT GNSTSGQRGN NRQIDIRGMG 100
    PENTLILIDG KPVSSRNSVR QGWRGERDTR GDTSWVPPEM IERIEVLRGP 150
    AAARYGNGAA GGVVNIITKK GSGEWHGSWD AYFNAPEHKE EGATKRTNFS 200
    LTGPLGDEFS FRLYGNLDKT QADAWDINQG HQSARAGTYA TTLPAGREGV 250
    INKDINGVVR WDFAPLQSLE LEAGYSRQGN LYAGDTQNTN SDSYTRSKYG 300
    DETNRLYRQN YALTWNGGWD NGVTTSNWVQ YEHTRNSRIP EGLAGGTEGK 350
    FNEKATQDFV DIDLDDVMLH SEVNLPIDFL VNQTLTLGTE WNQQRMKDLS 400
    SNTQALTGTN TGGAIDGVST TDRSPYSKAE IFSLFAENNM ELTDSTIVTP 450
    GLRFDHHSIV GNNWSPALNI SQGLGDDFTL KMGIARAYKA PSLYQTNPNY 500
    ILYSKGQGCY ASAGGCYLQG NDDLKAETSI NKEIGLEFKR DGWLAGVTWF 550
    RNDYRNKIEA GYVAVGQNAV GTDLYQWDNV PKAVVEGLEG SLNVPVSETV 600
    MWTNNITYML KSENKTTGDR LSIIPEYTLN STLSWQARED LSMQTTFTWY 650
    GKQQPKKYNY KGQPAVGPET KEISPYSIVG LSATWDVTKN VSLTGGVDNL 700
    FDKRLWRAGN AQTTGDLAGA NYIAGAGAYT YNEPGRTWYM SVNTHF 746
    Length:746
    Mass (Da):82,107
    Last modified:November 1, 1997 - v2
    Checksum:i09348AAB1C29710A
    GO

    Sequence cautioni

    The sequence AAB40783.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti152 – 1521A → R in AAA65994. (PubMed:3015941)Curated
    Sequence conflicti403 – 4031Missing in AAA65994. (PubMed:3015941)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13748 Genomic DNA. Translation: AAA65994.1.
    U82598 Genomic DNA. Translation: AAB40783.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC73685.1.
    AP009048 Genomic DNA. Translation: BAA35225.1.
    J04216 Genomic DNA. Translation: AAA23756.1.
    PIRiF64791. QRECFC.
    RefSeqiNP_415116.1. NC_000913.3.
    YP_488872.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73685; AAC73685; b0584.
    BAA35225; BAA35225; BAA35225.
    GeneIDi12934080.
    945193.
    KEGGiecj:Y75_p0572.
    eco:b0584.
    PATRICi32116338. VBIEscCol129921_0609.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13748 Genomic DNA. Translation: AAA65994.1 .
    U82598 Genomic DNA. Translation: AAB40783.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC73685.1 .
    AP009048 Genomic DNA. Translation: BAA35225.1 .
    J04216 Genomic DNA. Translation: AAA23756.1 .
    PIRi F64791. QRECFC.
    RefSeqi NP_415116.1. NC_000913.3.
    YP_488872.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FEP X-ray 2.40 A 23-746 [» ]
    ProteinModelPortali P05825.
    SMRi P05825. Positions 33-746.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9592N.
    IntActi P05825. 1 interaction.
    STRINGi 511145.b0584.

    Protein family/group databases

    TCDBi 1.B.14.1.22. the outer membrane receptor (omr) family.

    2D gel databases

    SWISS-2DPAGE P05825.

    Proteomic databases

    PaxDbi P05825.
    PRIDEi P05825.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73685 ; AAC73685 ; b0584 .
    BAA35225 ; BAA35225 ; BAA35225 .
    GeneIDi 12934080.
    945193.
    KEGGi ecj:Y75_p0572.
    eco:b0584.
    PATRICi 32116338. VBIEscCol129921_0609.

    Organism-specific databases

    EchoBASEi EB0289.
    EcoGenei EG10293. fepA.

    Phylogenomic databases

    eggNOGi COG4771.
    HOGENOMi HOG000276827.
    KOi K16089.
    OMAi TNTYAQY.
    OrthoDBi EOG68H83J.
    PhylomeDBi P05825.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10293-MONOMER.
    ECOL316407:JW5086-MONOMER.
    MetaCyc:EG10293-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P05825.
    PROi P05825.

    Gene expression databases

    Genevestigatori P05825.

    Family and domain databases

    Gene3Di 2.170.130.10. 1 hit.
    2.40.170.20. 1 hit.
    InterProi IPR012910. Plug.
    IPR000531. TonB-dep_rcpt_b-brl.
    IPR010916. TonB_box_CS.
    IPR010917. TonB_rcpt_CS.
    IPR010105. TonB_sidphr_rcpt.
    [Graphical view ]
    Pfami PF07715. Plug. 1 hit.
    PF00593. TonB_dep_Rec. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01783. TonB-siderophor. 1 hit.
    PROSITEi PS00430. TONB_DEPENDENT_REC_1. 1 hit.
    PS01156. TONB_DEPENDENT_REC_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the gene for the ferrienterochelin receptor FepA in Escherichia coli. Homology among outer membrane receptors that interact with TonB."
      Lundrigan M.D., Kadner R.J.
      J. Biol. Chem. 261:10797-10801(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter."
      Pettis G.S., Brickman T.J., McIntosh M.A.
      J. Biol. Chem. 263:18857-18863(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
    7. "The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product."
      Armstrong S.K., Pettis G.S., Forrester L.J., McIntosh M.A.
      Mol. Microbiol. 3:757-766(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 735-746.
    8. "Molecular analysis of the Escherichia coli ferric enterobactin receptor FepA."
      Armstrong S.K., Francis C.L., McIntosh M.A.
      J. Biol. Chem. 265:14536-14543(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: MOLECULAR ANALYSIS.
    9. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    10. "Crystal structure of the outer membrane active transporter FepA from Escherichia coli."
      Buchanan S.K., Smith B.S., Venkatramani L., Xia D., Esser L., Palnitkar M., Chakraborty R., van der Helm D., Deisenhofer J.
      Nat. Struct. Biol. 6:56-63(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

    Entry informationi

    Entry nameiFEPA_ECOLI
    AccessioniPrimary (citable) accession number: P05825
    Secondary accession number(s): P75722, P76821, P77093
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3