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Protein

DNA repair protein RecN

Gene

recN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in recombinational repair of damaged DNA.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 368ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • double-strand break repair Source: EcoCyc
  • double-strand break repair via homologous recombination Source: EcoCyc
  • SOS response Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10831-MONOMER.
ECOL316407:JW5416-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RecN
Alternative name(s):
Recombination protein N
Gene namesi
Name:recN
Synonyms:radB
Ordered Locus Names:b2616, JW5416
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10831. recN.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553DNA repair protein RecNPRO_0000188017Add
BLAST

Proteomic databases

PaxDbiP05824.

Expressioni

Inductioni

Induced by hydroxyurea.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ydcPP761042EBI-548098,EBI-556974

Protein-protein interaction databases

BioGridi4263399. 117 interactions.
DIPiDIP-10655N.
IntActiP05824. 2 interactions.
MINTiMINT-1269449.
STRINGi511145.b2616.

Structurei

3D structure databases

ProteinModelPortaliP05824.
SMRiP05824. Positions 2-552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecN family.Curated

Phylogenomic databases

eggNOGiENOG4105C4F. Bacteria.
COG0497. LUCA.
HOGENOMiHOG000009327.
InParanoidiP05824.
KOiK03631.
OMAiQVICVTH.
OrthoDBiEOG6WQD34.
PhylomeDBiP05824.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR004604. DNA_recomb/repair_RecN.
IPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
[Graphical view]
PANTHERiPTHR11059. PTHR11059. 1 hit.
PfamiPF02463. SMC_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003128. RecN. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00634. recN. 1 hit.

Sequencei

Sequence statusi: Complete.

P05824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAQLTISNF AIVRELEIDF HSGMTVITGE TGAGKSIAID ALGLCLGGRA
60 70 80 90 100
EADMVRTGAA RADLCARFSL KDTPAALRWL EENQLEDGHE CLLRRVISSD
110 120 130 140 150
GRSRGFINGT AVPLSQLREL GQLLIQIHGQ HAHQLLTKPE HQKFLLDGYA
160 170 180 190 200
NETSLLQEMT ARYQLWHQSC RDLAHHQQLS QERAARAELL QYQLKELNEF
210 220 230 240 250
NPQPGEFEQI DEEYKRLANS GQLLTTSQNA LALMADGEDA NLQSQLYTAK
260 270 280 290 300
QLVSELIGMD SKLSGVLDML EEATIQIAEA SDELRHYCDR LDLDPNRLFE
310 320 330 340 350
LEQRISKQIS LARKHHVSPE ALPQYYQSLL EEQQQLDDQA DSQETLALAV
360 370 380 390 400
TKHHQQALEI ARALHQQRQQ YAEELAQLIT DSMHALSMPH GQFTIDVKFD
410 420 430 440 450
EHHLGADGAD RIEFRVTTNP GQPMQPIAKV ASGGELSRIA LAIQVITARK
460 470 480 490 500
METPALIFDE VDVGISGPTA AVVGKLLRQL GESTQVMCVT HLPQVAGCGH
510 520 530 540 550
QHYFVSKETD GAMTETHMQS LNKKARLQEL ARLLGGSEVT RNTLANAKEL

LAA
Length:553
Mass (Da):61,396
Last modified:October 1, 1996 - v2
Checksum:i0048034F3515EDDB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 602AA → C in CAA68435 (PubMed:3037486).Curated
Sequence conflicti76 – 761A → R in CAA68435 (PubMed:3037486).Curated
Sequence conflicti535 – 55319GGSEV…ELLAA → VAVKSHVIHWRMRKNCLQRK LFSCFTVRVNSKTP in CAA68435 (PubMed:3037486).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00357 Genomic DNA. Translation: CAA68435.1.
U36840 Genomic DNA. Translation: AAA79786.1.
U00096 Genomic DNA. Translation: AAT48145.1.
AP009048 Genomic DNA. Translation: BAA16501.1.
PIRiC65040. RQECN.
RefSeqiWP_000880910.1. NZ_LN832404.1.
YP_026172.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48145; AAT48145; b2616.
BAA16501; BAA16501; BAA16501.
GeneIDi947105.
KEGGiecj:JW5416.
eco:b2616.
PATRICi32120631. VBIEscCol129921_2714.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00357 Genomic DNA. Translation: CAA68435.1.
U36840 Genomic DNA. Translation: AAA79786.1.
U00096 Genomic DNA. Translation: AAT48145.1.
AP009048 Genomic DNA. Translation: BAA16501.1.
PIRiC65040. RQECN.
RefSeqiWP_000880910.1. NZ_LN832404.1.
YP_026172.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP05824.
SMRiP05824. Positions 2-552.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263399. 117 interactions.
DIPiDIP-10655N.
IntActiP05824. 2 interactions.
MINTiMINT-1269449.
STRINGi511145.b2616.

Proteomic databases

PaxDbiP05824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48145; AAT48145; b2616.
BAA16501; BAA16501; BAA16501.
GeneIDi947105.
KEGGiecj:JW5416.
eco:b2616.
PATRICi32120631. VBIEscCol129921_2714.

Organism-specific databases

EchoBASEiEB0824.
EcoGeneiEG10831. recN.

Phylogenomic databases

eggNOGiENOG4105C4F. Bacteria.
COG0497. LUCA.
HOGENOMiHOG000009327.
InParanoidiP05824.
KOiK03631.
OMAiQVICVTH.
OrthoDBiEOG6WQD34.
PhylomeDBiP05824.

Enzyme and pathway databases

BioCyciEcoCyc:EG10831-MONOMER.
ECOL316407:JW5416-MONOMER.

Miscellaneous databases

PROiP05824.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR004604. DNA_recomb/repair_RecN.
IPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
[Graphical view]
PANTHERiPTHR11059. PTHR11059. 1 hit.
PfamiPF02463. SMC_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003128. RecN. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00634. recN. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and LexA regulation of the Escherichia coli recN gene."
    Rostas K., Morton S.J., Picksley S.M., Lloyd R.G.
    Nucleic Acids Res. 15:5041-5049(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: SEQUENCE REVISION TO 426.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia coli."
    Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J., Walker G.C.
    Mol. Cell 36:845-860(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HYDROXYUREA.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  7. Cited for: SUBCELLULAR LOCATION.
    Strain: K12.

Entry informationi

Entry nameiRECN_ECOLI
AccessioniPrimary (citable) accession number: P05824
Secondary accession number(s): P76602
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 1, 1996
Last modified: April 13, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.